题名 高效安全的核酸染料在琼脂糖凝胶电泳中的应用
被引量：5
1
作者
杨萍 陆嘉伟 李翠环
机构
浙江农林大学省部共建亚热带森林培育国家重点实验室
出处
《实验室研究与探索》
CAS
北大核心
2022年第7期60-64,共5页
基金
浙江农林大学实验室研究重点项目(JS20190003)。
文摘
核酸染料的高效安全性一直是高校生物类实验室关注重点。从染色效果、灵敏度、染色方式、安全性以及性价比等方面探讨了溴化乙锭(EB)和9种新型核酸染料在琼脂糖凝胶电泳中的应用。结果表明,采用胶染法0.2×GelRed可以检测到5 ng DL5000DNA Marker所有DNA片段,显示的DNA电泳条带平整、清晰易辨;GelRed具有高灵敏性、无毒性且性价比合理,可作为EB替代染料在琼脂糖凝胶电泳中推广应用。
关键词
核酸染料 琼脂糖凝胶电泳 高效 安全性
Keywords
nucleic acid dye agarose gel electrophoresishigh efficiencysafety
分类号
TM132
[电气工程—电工理论与新技术]
题名 体外酶法水解制备小分子透明质酸
被引量：1
2
作者
原攀红 康振 金鹏 刘龙 堵国成 陈坚
机构
江南大学生物工程学院 江南大学食品科学与技术国家重点实验室
出处
《食品科学技术学报》
CAS
2016年第2期51-55,82,共6页
文摘
为了制备分子量分布集中的小分子透明质酸,在6 g/L的高分子量透明质酸水溶液中加入一定梯度重组水蛭透明质酸酶,并控制水解时间。结果表明,通过控制加入重组水蛭HAase的量和水解时间,根据水解分子量下降规律,可以制备出分子量分布集中的小分子HA,包括水解终产物透明质酸四糖和六糖。同时,通过高效体积排阻色谱和琼脂糖凝胶电泳,可以证明水解产物的分子量分布集中。
关键词
透明质酸 重组水蛭透明质酸酶 小分子透明质酸 高效体积排阻色谱 琼脂糖凝胶电泳
Keywords
hyaluronan recombinant leech hyaluronidase small molecular hyaluronan high perform-ance size exclusion chromatography agarose gel electrophoresis
分类号
TS202.1
[轻工技术与工程—食品科学]
题名 中韩野生软枣猕猴桃种质资源遗传多样性分析
被引量：13
3
作者
赵成日
机构
延边大学农学院
出处
《果树学报》
CAS
CSCD
北大核心
2018年第9期1043-1051,共9页
基金
国家自然科学基金(31660319)
文摘
【目的】利用RAPD分子标记技术研究国内长白山地区和韩国由来野生软枣猕猴桃种质资源的遗传多样性。【方法】以长白山地区和韩国由来的28个野生软枣猕猴桃的叶片为材料,利用RAPD分子标记技术进行了遗传多样性分析,并明确了它们之间的亲缘关系。【结果】利用131个随机引物进行PCR,从中筛选出了多态性高、重复性好且扩增条带清晰的24个引物。24个引物在28个野生软枣猕猴桃中共扩增出191条带,其中多态性条带为186条,占97.4%。平均每个引物产生7.75个多态性条带。应用NTSYSpc 2.10e软件进行遗传一致度和遗传距离分析后用UPGMA方法进行聚类分析。28个野生软枣猕猴桃种质资源之间的遗传距离为0.020 2~0.934 2。遗传距离0.58时可将28个野生种划分成2大类。在遗传距离最小的0.02处,有蛟河2号和3号2个野生种,其RAPD分子标记相似性为98%。【结论】来自不同地理区域的野生软枣猕猴桃之间存在较高的遗传多样性,而在同一地理区域内遗传多样性较低。韩国野生软枣猕猴桃之间的遗传多样性较低,且与二道白河、汪清、左家等地的野生软枣猕猴桃亲缘关系较近。即具有同一地理区域聚类趋势,且不同地理区域间存在较高的遗传多样性。
关键词
软枣猕猴桃 遗传多样性 RAPD 长白山地区 韩国
Keywords
Actinidia arguta Gendenaturation at 94 ℃ for 1 min annealing at 36 ℃for 1 min extension at 72 ℃ for 2 min reaction of 40 cycles last extension at 72 ℃ for 5 min. The same template was repeated twice. RAPD-PCR amplification products were analyzed by 0.8% agarose gel （0.5 ×TAE buffer） electrophoresis. [Results]The 24 primers had high genetic polymorphisms. A total of 191 bands were amplified of which 186 were polymorphic bands accounting for 97.4%. Average 7.75 polymorphic bands were produced by each primer. Genetic identity and genetic distance were calculated by NTSYSpc 2.10e software and cluster analysis was analyzed by UPGMA. The result showed that the genetic distances among the 28 wild species ofA. arguta germplasm resources were 0.020 2-0.934 2. When the genetic distance was 0.58 the 28 wild species could be divided into two groups. The first group included Antu coloring type Antu No.1-6 fromAntu County and Jiaohe No. 1-5 from Jiaohe. The second group include： ErdaobaiheNo. 1-4 from Erdaobaihe Wangqing No. 1-3 from Wangqing County and Wangqing Forestry Bureau Zuojia No. 1-3 from the Zuojia special production Institute of the Chinese Academy of Agricultural Sciences （CAAS） Liaoning Huanren and Helong Qingshan South Korea No. 1-4 from Chungbuk Jeon- ham and Gyeongnam in South Korea. The similarity of RAPD markers of Jiaohe 2 and 3 the two wild species reached 98 % when the genetic distance was 0.0202. Almost all the wild species from the same area belonged to the same category within a very small genetic distance. For example Antu No. 1-6 were within the 0.184 genetic distance Jiaohe No. 1-5 were within 0.18 genetic distance Wangqing No. 1-3 and Erdaobaihe No. 1 and No. 4 were within 0.315 genetic distance Zuojia No. 1-3 was within 0.25 genetic distance South Korea No. 1-4 were within 0.23 genetic distance Erdaobaihe No. 2 and No. 3 were within 0.41 genetic distance. [Conclusion]There was a high genetic diversity among wild A. arguta collected from different geographic regions but genetic diversity among those collected from the same geographical area was low. The genetic diversity of the wild A. arguta from South Korea was relatively low and was closely related to the wild A. arguta from Erdaobaihe Wang Qing and Zuo Jia. The species collected from the etic diversity RAPD Changbai mountion South Korea
分类号
S663.4
[农业科学—果树学]
