Hepatitis B virus(HBV)DNA and HBsAg were detected in 51 human paraf- fin-embedded sections of liver cirrhosis by the double labelling technique of in situ hybfidiza- tion and PAP method.The results showed that the pos...Hepatitis B virus(HBV)DNA and HBsAg were detected in 51 human paraf- fin-embedded sections of liver cirrhosis by the double labelling technique of in situ hybfidiza- tion and PAP method.The results showed that the positive sections were 21(41.2％)for HBV DNA,43(84.3％)for HBsAg and 19(37.3％)for both HBV DNA and HBsAg.HBV DNA-positive grains were localized predominantly in the cytoplasm of hepatic cells or in both nucleus and cytoplasm,a few only in the nucleus or the inner side of the cell membrane.In active cirrhosis and inactive cirrhosis,the positive rates of HBV DNA were 52.8％ and 13.3％,respectively,and for both HBV DNA and HBsAg,they were 47.2％ and 13.3％ restively.The positive rate is higher in active cirrhosis than in inactive cirrhosis(P<0.05).The results further indicated that the infection of HBV and the existence and persistent action of HBV DNA in the liver tissues are one of the important factors of the development of liver cirrhosis.展开更多
文摘Hepatitis B virus(HBV)DNA and HBsAg were detected in 51 human paraf- fin-embedded sections of liver cirrhosis by the double labelling technique of in situ hybfidiza- tion and PAP method.The results showed that the positive sections were 21(41.2％)for HBV DNA,43(84.3％)for HBsAg and 19(37.3％)for both HBV DNA and HBsAg.HBV DNA-positive grains were localized predominantly in the cytoplasm of hepatic cells or in both nucleus and cytoplasm,a few only in the nucleus or the inner side of the cell membrane.In active cirrhosis and inactive cirrhosis,the positive rates of HBV DNA were 52.8％ and 13.3％,respectively,and for both HBV DNA and HBsAg,they were 47.2％ and 13.3％ restively.The positive rate is higher in active cirrhosis than in inactive cirrhosis(P<0.05).The results further indicated that the infection of HBV and the existence and persistent action of HBV DNA in the liver tissues are one of the important factors of the development of liver cirrhosis.
