BACKGROUND:Hyperbaric oxygen(HBO)is an effective adjuvant therapy for ischemiareperfusion(I/R)injury of the brain,small intestine and testis in addition to crushing injury.Studies have shown that HBO increases the act...BACKGROUND:Hyperbaric oxygen(HBO)is an effective adjuvant therapy for ischemiareperfusion(I/R)injury of the brain,small intestine and testis in addition to crushing injury.Studies have shown that HBO increases the activity of villi of the ileum 30 minutes after I/R injury.The present study aimed to observe the effect of HBO on apoptosis of epithelial cells in the small intestine during different periods of I/R and to elucidate the potential mechanisms.METHODS:Rats were subjected to 60-minute ischemia by clamping the superior mesenteric artery and 60-minute reperfusion by removal of clamping.The rats were randomly divided into four groups:I/R group,HBO precondition or HBO treatment before ischemia(HBO-P),HBO treatment during ischemia period(HBO-I),and HBO treatment during reperfusion(HBO-R).After 60-minute reperfusion,samples of the small intestine were prepared to measure the level of ATP by using the colorimetric method and immunochemical expression of caspase-3.The levels of TNF-αin intestinal tissue were measured using the enzyme-linked immunosorbent assay method(Elisa).RESULTS:TNF-αlevels were significantly lower in the HBO-I group than in the HBO-P(P<0.05),HBO-R and I/R groups;there was no significant difference between the HBO-R and I/R groups(P>0.05).The expression of caspas-3 was significantly lower in the HBO-I group than in the HBO-P group(P<0.05);it was also significantly lower in the HBO-P group than in the I/R and HBO-R groups(P<0.05).ATP level was significantly lower in the HBO-I group than in the HBO-P group(P<0.05),and also it was significantly lower in the HBO-P group than in the I/R and HBO-R groups(P<0.05).CONCLUSIONS:There is an association between HBO,small intestinal I/R injury,and mucosa apoptosis.HBO maintains ATP and aerobic metabolism,inhibites TNF-αproduction,and thus prevents intestinal mucosa from apoptosis.Best results can be obtained when HBO is administered to patients in the period of ischemia,and no side effects are produced when HBO is given during the Period of Reperfusion.展开更多
A female patient at the age of 17 received dilator implant on the right part of her face to prepare skin flap for her facial scar.The skin flap was in good conditions before scar cutting,but showed poor blood circulat...A female patient at the age of 17 received dilator implant on the right part of her face to prepare skin flap for her facial scar.The skin flap was in good conditions before scar cutting,but showed poor blood circulation after being transferred.Hyperbaric oxygen therapy was given at 0.2 MPa(2ATA) in double pure oxygen cabins for 90 min every day for 10 d.The skin flap showed in sound viability and sound blood circulation after the treatment.Hyperbaric oxygen therapy can be used to treat blood circulation disorder in transferred skin flap.展开更多
This report describes for the first time the effects of hyperbaric oxygen therapy with 253kPa on patients(n=24) with post - trauma acute compartment syndrome, and the relationship be-tween the prognosis of the syndrom...This report describes for the first time the effects of hyperbaric oxygen therapy with 253kPa on patients(n=24) with post - trauma acute compartment syndrome, and the relationship be-tween the prognosis of the syndrome and the changes of plasma fibronectin. Plasma fibronectin wasmeasured using single radial immunodiffusion both before and after therapy with hyperbaric oxygen,with 30 healthy people as control for plasma fibronectin. The results showed that the clinical symp-toms and signs of all 16 patients with acute compartment syndromes in early stage were significantlyimproved after 3 times of hyperbaric oxygen therapy and disappeared after 5 times withoutfasciotomy. Six patients who were treated with hyperbaric oxygen after fasciotomy showed reductionin the infection, and, edema of the injured limb However, the effects were less favorable for 2 pa-tients with necrosis although their clinical symptoms and signs were partially improved. Besides,we found that these patients’ plasma fibronectin concentrations were significantly afterhyperbaric oxygen therapy. The mean increase was 43 (18. 9％; P【0. 01), 61( 24. 7%, P【0. 01),49 (17. 2%, P【0. 01), 57 (17. 4%; P【0. 05) and 55 mg/L ( 16. 6%; P【0. 05) after 1, 2, 3, 4and 5 times of therapy, respectively. Plasma fibronectin concentrations returned to the level of thecontrols after 3 times of therapy (P】0. 05). After fourth and fifth therapy plasma fibronectin lev-els of the patients rose higher than those of the controls (P【0.05). The results suggest that hyperbaric oxygen therapy has a beneficial effect on patients withacute compartment syndrome in the early stage Hyperbaric oxygen therapy can reduce edema andskeletal muscle necrosis The method can be used to treat patients with acute compartmentsyndrome in early stage and as an effective adjunctive treatment after fasciotomy.展开更多
The content of somatostatin(SS) in hippocampus,striatum and frontal cortex tissues of rats exposed to 600 kpa hyperbaric oxygen was determined by means of radioimmunoassay. Initial time of convulsion, severity of conv...The content of somatostatin(SS) in hippocampus,striatum and frontal cortex tissues of rats exposed to 600 kpa hyperbaric oxygen was determined by means of radioimmunoassay. Initial time of convulsion, severity of convulsion and survival time of rats with convulsion exposed to 700 kPa hyperbaric oxygen after intraperitoneal injection of cysteamine (CSH) or intracerebroventricular injection of anti-somatostatin serum(ASS) were also observed. The results showed that the content of SS in hippocampus and striatum tissues increased remarkably when rats were at near-convulsion ; by the time the rats developed convulsion,it had a significant increase in all brain areas observed. Intraperitoneal injection of CSH or intracerebroventricular injection of ASS could delay initial time of convulsion (ITC),prolong survival time (ST) and reduce severity of convulsion (SOC). These results suggest that SS might play a role in oxygen-induced convulsion and be one of the endogenous agents which caused oxygeninduced convulsion.展开更多
文摘BACKGROUND:Hyperbaric oxygen(HBO)is an effective adjuvant therapy for ischemiareperfusion(I/R)injury of the brain,small intestine and testis in addition to crushing injury.Studies have shown that HBO increases the activity of villi of the ileum 30 minutes after I/R injury.The present study aimed to observe the effect of HBO on apoptosis of epithelial cells in the small intestine during different periods of I/R and to elucidate the potential mechanisms.METHODS:Rats were subjected to 60-minute ischemia by clamping the superior mesenteric artery and 60-minute reperfusion by removal of clamping.The rats were randomly divided into four groups:I/R group,HBO precondition or HBO treatment before ischemia(HBO-P),HBO treatment during ischemia period(HBO-I),and HBO treatment during reperfusion(HBO-R).After 60-minute reperfusion,samples of the small intestine were prepared to measure the level of ATP by using the colorimetric method and immunochemical expression of caspase-3.The levels of TNF-αin intestinal tissue were measured using the enzyme-linked immunosorbent assay method(Elisa).RESULTS:TNF-αlevels were significantly lower in the HBO-I group than in the HBO-P(P<0.05),HBO-R and I/R groups;there was no significant difference between the HBO-R and I/R groups(P>0.05).The expression of caspas-3 was significantly lower in the HBO-I group than in the HBO-P group(P<0.05);it was also significantly lower in the HBO-P group than in the I/R and HBO-R groups(P<0.05).ATP level was significantly lower in the HBO-I group than in the HBO-P group(P<0.05),and also it was significantly lower in the HBO-P group than in the I/R and HBO-R groups(P<0.05).CONCLUSIONS:There is an association between HBO,small intestinal I/R injury,and mucosa apoptosis.HBO maintains ATP and aerobic metabolism,inhibites TNF-αproduction,and thus prevents intestinal mucosa from apoptosis.Best results can be obtained when HBO is administered to patients in the period of ischemia,and no side effects are produced when HBO is given during the Period of Reperfusion.
文摘A female patient at the age of 17 received dilator implant on the right part of her face to prepare skin flap for her facial scar.The skin flap was in good conditions before scar cutting,but showed poor blood circulation after being transferred.Hyperbaric oxygen therapy was given at 0.2 MPa(2ATA) in double pure oxygen cabins for 90 min every day for 10 d.The skin flap showed in sound viability and sound blood circulation after the treatment.Hyperbaric oxygen therapy can be used to treat blood circulation disorder in transferred skin flap.
文摘This report describes for the first time the effects of hyperbaric oxygen therapy with 253kPa on patients(n=24) with post - trauma acute compartment syndrome, and the relationship be-tween the prognosis of the syndrome and the changes of plasma fibronectin. Plasma fibronectin wasmeasured using single radial immunodiffusion both before and after therapy with hyperbaric oxygen,with 30 healthy people as control for plasma fibronectin. The results showed that the clinical symp-toms and signs of all 16 patients with acute compartment syndromes in early stage were significantlyimproved after 3 times of hyperbaric oxygen therapy and disappeared after 5 times withoutfasciotomy. Six patients who were treated with hyperbaric oxygen after fasciotomy showed reductionin the infection, and, edema of the injured limb However, the effects were less favorable for 2 pa-tients with necrosis although their clinical symptoms and signs were partially improved. Besides,we found that these patients’ plasma fibronectin concentrations were significantly afterhyperbaric oxygen therapy. The mean increase was 43 (18. 9％; P【0. 01), 61( 24. 7%, P【0. 01),49 (17. 2%, P【0. 01), 57 (17. 4%; P【0. 05) and 55 mg/L ( 16. 6%; P【0. 05) after 1, 2, 3, 4and 5 times of therapy, respectively. Plasma fibronectin concentrations returned to the level of thecontrols after 3 times of therapy (P】0. 05). After fourth and fifth therapy plasma fibronectin lev-els of the patients rose higher than those of the controls (P【0.05). The results suggest that hyperbaric oxygen therapy has a beneficial effect on patients withacute compartment syndrome in the early stage Hyperbaric oxygen therapy can reduce edema andskeletal muscle necrosis The method can be used to treat patients with acute compartmentsyndrome in early stage and as an effective adjunctive treatment after fasciotomy.
文摘The content of somatostatin(SS) in hippocampus,striatum and frontal cortex tissues of rats exposed to 600 kpa hyperbaric oxygen was determined by means of radioimmunoassay. Initial time of convulsion, severity of convulsion and survival time of rats with convulsion exposed to 700 kPa hyperbaric oxygen after intraperitoneal injection of cysteamine (CSH) or intracerebroventricular injection of anti-somatostatin serum(ASS) were also observed. The results showed that the content of SS in hippocampus and striatum tissues increased remarkably when rats were at near-convulsion ; by the time the rats developed convulsion,it had a significant increase in all brain areas observed. Intraperitoneal injection of CSH or intracerebroventricular injection of ASS could delay initial time of convulsion (ITC),prolong survival time (ST) and reduce severity of convulsion (SOC). These results suggest that SS might play a role in oxygen-induced convulsion and be one of the endogenous agents which caused oxygeninduced convulsion.
