With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a c...With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.展开更多
The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary ...The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary to make clear the molecular genetic characteristics,edible safety,planting,processing,and other aspects of the safety evaluation of GM crops.The safety problems existing in the cultivation of GM crops,safety evaluation and detection of GM crops were introduced in this paper,which provided the basis for safety evaluation and effective supervision of GM crops and their products.Commercial cultivation and reasonable supervision based on safety evaluation have far-reaching significance for ensuring consumer safety,enhancing the credibility of the national political system and enhancing citizens'confidence in the safety of GM crop products for consumption.展开更多
This paper presents an adaptive fuzzy control scheme based on modified genetic algorithm. In the control scheme, genetic algorithm is used to optimze the nonlinear quantization functions of the controller and some key...This paper presents an adaptive fuzzy control scheme based on modified genetic algorithm. In the control scheme, genetic algorithm is used to optimze the nonlinear quantization functions of the controller and some key parameters of the adaptive control algorithm. Simulation results show that this control scheme has satisfactory performance in MIMO systems, chaotic systems and delay systems.展开更多
In order to reduce both the weight of vehicles and the damage of occupants in a crash event simultaneously, it is necessary to perform a multi-objective optimal design of the automotive energy absorbing components. Mo...In order to reduce both the weight of vehicles and the damage of occupants in a crash event simultaneously, it is necessary to perform a multi-objective optimal design of the automotive energy absorbing components. Modified non-dominated sorting genetic algorithm II(NSGA II) was used for multi-objective optimization of automotive S-rail considering absorbed energy(E), peak crushing force(Fmax) and mass of the structure(W) as three conflicting objective functions. In the multi-objective optimization problem(MOP), E and Fmax are defined by polynomial models extracted using the software GEvo M based on train and test data obtained from numerical simulation of quasi-static crushing of the S-rail using ABAQUS. Finally, the nearest to ideal point(NIP)method and technique for ordering preferences by similarity to ideal solution(TOPSIS) method are used to find the some trade-off optimum design points from all non-dominated optimum design points represented by the Pareto fronts. Results represent that the optimum design point obtained from TOPSIS method exhibits better trade-off in comparison with that of optimum design point obtained from NIP method.展开更多
基金the Experimental Technology Research Project of Zhejiang University(SYB202138)National Natural Science Foundation of China(32000195)。
文摘With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants.
基金Supported by the China Postdoctoral Science Foundation(2023M730312)the Science and Technology Plan Projects of the State Administration for Market Regulation(2022MK002)the National Key Research and Development Program(2022YFF0606105)。
文摘The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary to make clear the molecular genetic characteristics,edible safety,planting,processing,and other aspects of the safety evaluation of GM crops.The safety problems existing in the cultivation of GM crops,safety evaluation and detection of GM crops were introduced in this paper,which provided the basis for safety evaluation and effective supervision of GM crops and their products.Commercial cultivation and reasonable supervision based on safety evaluation have far-reaching significance for ensuring consumer safety,enhancing the credibility of the national political system and enhancing citizens'confidence in the safety of GM crop products for consumption.
文摘This paper presents an adaptive fuzzy control scheme based on modified genetic algorithm. In the control scheme, genetic algorithm is used to optimze the nonlinear quantization functions of the controller and some key parameters of the adaptive control algorithm. Simulation results show that this control scheme has satisfactory performance in MIMO systems, chaotic systems and delay systems.
文摘In order to reduce both the weight of vehicles and the damage of occupants in a crash event simultaneously, it is necessary to perform a multi-objective optimal design of the automotive energy absorbing components. Modified non-dominated sorting genetic algorithm II(NSGA II) was used for multi-objective optimization of automotive S-rail considering absorbed energy(E), peak crushing force(Fmax) and mass of the structure(W) as three conflicting objective functions. In the multi-objective optimization problem(MOP), E and Fmax are defined by polynomial models extracted using the software GEvo M based on train and test data obtained from numerical simulation of quasi-static crushing of the S-rail using ABAQUS. Finally, the nearest to ideal point(NIP)method and technique for ordering preferences by similarity to ideal solution(TOPSIS) method are used to find the some trade-off optimum design points from all non-dominated optimum design points represented by the Pareto fronts. Results represent that the optimum design point obtained from TOPSIS method exhibits better trade-off in comparison with that of optimum design point obtained from NIP method.
