Total nucleosides in sporophore of \%Ganoderma lucidum \%(Leyss. ex Fr.) Karst produced in different locality was determinated by the UV\|spectrophotometry. The contents of total nucleosides in sporophore of \%G. luci...Total nucleosides in sporophore of \%Ganoderma lucidum \%(Leyss. ex Fr.) Karst produced in different locality was determinated by the UV\|spectrophotometry. The contents of total nucleosides in sporophore of \%G. lucidum \%from Guangxi of China and Viet Nam were 0.33%and 0.34%, the higher contents from Taiwan, Jiangsu and Guangdong were 0.40%～0.46% and lower contents from Yunnan were 0.16%～0.21%. The research proposed a criterion on the quality of \%G. lucidum \%and a brief discussion about its utilization and development. The TLC identification of uracil, adenine, adenosine and uridine in sporophore of \%G. lucidum \%was carried out. The methodological study indicated that the method was simple, reliable and specific.展开更多
以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表...以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表明,野生灵芝(D)与自然单核灵芝(Mo)菌丝体的差异表达基因主要富集在转录调控及DNA模板化、tRNA氨酰化、组蛋白甲基化等过程;野生灵芝(D)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、L-丝氨酸代谢、硫化合物代谢等过程;自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、DNA模板转录、谷氨酸分解等过程。KEGG富集结果表明,自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体在代谢通路上有明显的差异,差异基因主要被注释到过氧化物酶、维生素代谢等通路。野生灵芝(D)与自然单核灵芝(Mo)菌丝体、野生灵芝(D)与双核灵芝(Mu)菌丝体的差异基因并没有KEGG富集的结果,说明其代谢通路没有明显差异。筛选出3个品系中表达量差异均显著的基因,共12个,并对基因的主要功能进行预测。12个基因中有5个基因可以匹配到GO库。GO注释结果表明,这5个基因主要被注释到氧化还原酶活性、金属离子结合、膜的调控与膜组成等方面。展开更多
文摘分别利用紫外线和超高压诱变漆酶产生菌灵芝(Ganoderma.lucidum Karst),发现超高压的诱变幅度较大.经初步筛选和发酵,获得了2株具有较大应用潜力的漆酶超高压突变株G1502和G2001,前者漆酶活力比出发菌株提高了2.83倍,发酵时间缩短了1 d;后者漆酶活力提高了0.89倍,发酵时间缩短了3 d.
文摘Total nucleosides in sporophore of \%Ganoderma lucidum \%(Leyss. ex Fr.) Karst produced in different locality was determinated by the UV\|spectrophotometry. The contents of total nucleosides in sporophore of \%G. lucidum \%from Guangxi of China and Viet Nam were 0.33%and 0.34%, the higher contents from Taiwan, Jiangsu and Guangdong were 0.40%～0.46% and lower contents from Yunnan were 0.16%～0.21%. The research proposed a criterion on the quality of \%G. lucidum \%and a brief discussion about its utilization and development. The TLC identification of uracil, adenine, adenosine and uridine in sporophore of \%G. lucidum \%was carried out. The methodological study indicated that the method was simple, reliable and specific.
文摘以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表明,野生灵芝(D)与自然单核灵芝(Mo)菌丝体的差异表达基因主要富集在转录调控及DNA模板化、tRNA氨酰化、组蛋白甲基化等过程;野生灵芝(D)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、L-丝氨酸代谢、硫化合物代谢等过程;自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、DNA模板转录、谷氨酸分解等过程。KEGG富集结果表明,自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体在代谢通路上有明显的差异,差异基因主要被注释到过氧化物酶、维生素代谢等通路。野生灵芝(D)与自然单核灵芝(Mo)菌丝体、野生灵芝(D)与双核灵芝(Mu)菌丝体的差异基因并没有KEGG富集的结果,说明其代谢通路没有明显差异。筛选出3个品系中表达量差异均显著的基因,共12个,并对基因的主要功能进行预测。12个基因中有5个基因可以匹配到GO库。GO注释结果表明,这5个基因主要被注释到氧化还原酶活性、金属离子结合、膜的调控与膜组成等方面。
