Vacuum precision seed metering is the key part of vacuum seed planter. Planting performance of planter is affected by vacuum and air flow which are important parameters for choosing fan. Effects of qualification perce...Vacuum precision seed metering is the key part of vacuum seed planter. Planting performance of planter is affected by vacuum and air flow which are important parameters for choosing fan. Effects of qualification percent and miss percent on air chamber vacuum 3, 4, 5 and 6 kPa were studied at different operating speeds. The results showed that operating performance of the seed metering was excellent when air chamber vacuum was 5 and 6 kPa, which air flow was 7.4-8.0 m3·s-1 and 8.0-8.8 m3·s-1 , respectively.展开更多
[目的]为Pleurotus eryngii—Co60-7木质素降解酶的分离纯化和综合利用提供试验依据。[方法]采用DEAE—Sepharose^TM Fast Flow离子交换介质，分别考察缓冲液pH值、流速和洗脱方式等对刺芹侧耳木质素降解酶分离纯化的影响，确定了最佳...[目的]为Pleurotus eryngii—Co60-7木质素降解酶的分离纯化和综合利用提供试验依据。[方法]采用DEAE—Sepharose^TM Fast Flow离子交换介质，分别考察缓冲液pH值、流速和洗脱方式等对刺芹侧耳木质素降解酶分离纯化的影响，确定了最佳分离纯化层析条件。[结果]DEAE-Sephalose^TM Fast Flow分离纯化Pleurotus eryngii-Co60-7木质素降解酶的最佳层析条件为：选择20mmol／L，pH值为5．0醋酸钠一醋酸缓冲体系，3ml／min的流速，进行分步洗脱（100、200～300和1000mmoL／L NaCl的三步洗脱），可较好地实现刺芹侧耳发酵液木质素降解酶初分，该纯化操作目标蛋白回收率达85％，纯化分离因素为2．71。[结论]该技术在分离纯化刺芹侧耳木质素降解酶上可行，具有潜在的工业应用价值。展开更多
基金Supported by the Youth Science Foundation of Heilongjiang(QC2010119,QC2010028)the Science and Technology Innovation Person with Ability Study Special Foundation Project of Harbin(2011RFQXN054)+1 种基金the Graduate Student Innovative Scientific Research Project of Heilongjiang(YJSCX2011-064JHL)Division of Soybean Machinery,CARS(nycytx-004)
文摘Vacuum precision seed metering is the key part of vacuum seed planter. Planting performance of planter is affected by vacuum and air flow which are important parameters for choosing fan. Effects of qualification percent and miss percent on air chamber vacuum 3, 4, 5 and 6 kPa were studied at different operating speeds. The results showed that operating performance of the seed metering was excellent when air chamber vacuum was 5 and 6 kPa, which air flow was 7.4-8.0 m3·s-1 and 8.0-8.8 m3·s-1 , respectively.
文摘[目的]为Pleurotus eryngii—Co60-7木质素降解酶的分离纯化和综合利用提供试验依据。[方法]采用DEAE—Sepharose^TM Fast Flow离子交换介质，分别考察缓冲液pH值、流速和洗脱方式等对刺芹侧耳木质素降解酶分离纯化的影响，确定了最佳分离纯化层析条件。[结果]DEAE-Sephalose^TM Fast Flow分离纯化Pleurotus eryngii-Co60-7木质素降解酶的最佳层析条件为：选择20mmol／L，pH值为5．0醋酸钠一醋酸缓冲体系，3ml／min的流速，进行分步洗脱（100、200～300和1000mmoL／L NaCl的三步洗脱），可较好地实现刺芹侧耳发酵液木质素降解酶初分，该纯化操作目标蛋白回收率达85％，纯化分离因素为2．71。[结论]该技术在分离纯化刺芹侧耳木质素降解酶上可行，具有潜在的工业应用价值。
