植物硝酸盐转运蛋白(Nitrate transporter,NRT)可有效转运NO-3,提升氮素利用效率。为了解析草地早熟禾(Poa pratensis)适应不同浓度及不同形态氮素、干旱胁迫机理,本研究以草地早熟禾为试材,对NRT1/PTR FAMILY 8.3基因进行克隆、生物信...植物硝酸盐转运蛋白(Nitrate transporter,NRT)可有效转运NO-3,提升氮素利用效率。为了解析草地早熟禾(Poa pratensis)适应不同浓度及不同形态氮素、干旱胁迫机理,本研究以草地早熟禾为试材,对NRT1/PTR FAMILY 8.3基因进行克隆、生物信息学分析,并分析了不同浓度及不同形态氮素以及干旱下该基因表达情况。研究结果:草地早熟禾NRT1/PTR FAMILY 8.3基因包含典型的主要协同转运蛋白超家族(Major facilitator superfamily,MFS)结构域,与二穗短柄草(Brachypodium distachyon)高度同源;荧光定量PCR分析表明,该基因根部、叶部的表达量显著高于茎部;氮浓度为7.5 mM时,不同形态氮素诱导下,NaNO3处理组有利于该基因表达;无氮、高氮组(0,15 mM NaNO3)基因的表达量显著高于适氮组(7.5 mM NaNO3);干旱胁迫可促进其表达。研究结果为探究NRT1/PTR FAMILY 8.3在不同氮素环境中的调节机制,培育氮素利用率高的优质草种提供理论基础。展开更多
慢性病患者的长期管理是基层医疗的核心任务之一,患者的规律随访(TR)与疾病预后密切相关。近期Annals of Family Medicine刊发了一篇文章《Teamwork among Primary Care Staff to Achieve Regular Follow-Up of Chronic Patients》,该...慢性病患者的长期管理是基层医疗的核心任务之一,患者的规律随访(TR)与疾病预后密切相关。近期Annals of Family Medicine刊发了一篇文章《Teamwork among Primary Care Staff to Achieve Regular Follow-Up of Chronic Patients》,该文章指出慢性病患者的TR面临多重挑战,既往研究多聚焦于患者个体因素,而忽视了医疗团队动态对随访率的潜在影响。展开更多
Creutzfeldt-Jakob disease(CJD)is a rare neurodegenerative disorder characterized by abnormalities in the prion protein(PrP),the most common form of human prion disease.Although Genome-Wide Association Studies(GWAS)hav...Creutzfeldt-Jakob disease(CJD)is a rare neurodegenerative disorder characterized by abnormalities in the prion protein(PrP),the most common form of human prion disease.Although Genome-Wide Association Studies(GWAS)have identified numerous risk genes for CJD,the mechanisms underlying these risk loci remain poorly understood.This study aims to elucidate novel genetically prioritized candidate proteins associated with CJD in the human brain through an integrative analytical pipeline.Utilizing datasets from Protein Quantitative Trait Loci(pQTL)(NpQTL1=152,NpQTL2=376),expression QTL(eQTL)(N=452),and the CJD GWAS(NCJD=4110,NControls=13569),we implemented a systematic analytical pipeline.This pipeline included Proteome-Wide Association Study(PWAS),Mendelian randomization(MR),Bayesian colocalization,and Transcriptome-Wide Association Study(TWAS)to identify novel genetically prioritized candidate proteins implicated in CJD pathogenesis within the brain.Through PWAS,we identified that the altered abundance of six brain proteins was significantly associated with CJD.Two genes,STX6 and PDIA4,were established as lead causal genes for CJD,supported by robust evidence(False Discovery Rate<0.05 in MR analysis;PP4/(PP3+PP4)≥0.75 in Bayesian colocalization).Specifically,elevated levels of STX6 and PDIA4 were associated with an increased risk of CJD.Additionally,TWAS demonstrated that STX6 and PDIA4 were associated with CJD at the transcriptional level.展开更多
The Myb family of proteins is a group offunctionally diverse transcriptional activatorsfound in both plants and animals that ischaracterized by a conserved DNA bindingdomain of approximately 50 amino acids.Inplants,My...The Myb family of proteins is a group offunctionally diverse transcriptional activatorsfound in both plants and animals that ischaracterized by a conserved DNA bindingdomain of approximately 50 amino acids.Inplants,Myb proteins are involved in control展开更多
MicroRNA（miRNA） is a class of important regulating non-coding small molecular RNA. The gma-MIR166 gene family consists of 21 members and their expression patterns diversify widely. It is important to analyze the evo...MicroRNA（miRNA） is a class of important regulating non-coding small molecular RNA. The gma-MIR166 gene family consists of 21 members and their expression patterns diversify widely. It is important to analyze the evolution of gma-MIR166 gene family in order to understand the evolutionary mechanisms of miRNAs in soybean. In this study, we implemented soybean wide genome block analysis, the molecular phylogeny of gma-MIR166 and block analysis of gma-MIR166 family. The results showed that both chromosome big segmental duplications and tandem duplications were main reasons contributed to the expanding of gmaMIR166 gene family. These findings suggested that gma-MIR166 gene family might originate from one or two ancient miRNA genes. The results of research provided a support for evolutionary study of miRNAs in soybean and related species in Fabaceae.展开更多
Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to dam...Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to damage in cotton growth and a decrease in yields.Hyperosmolality-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration.Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants.However,the molecular nature of the corresponding calcium ion channels remains unclearly.In this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse genetics.Result:A total of 35,21 and 22 OSCA genes were identified in G.hirsutum,G.arboreum,and G.raimondii genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic relationship.Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) domain.Moreover,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress.Furthermore,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants.Conclusion:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton.These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes,thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions.展开更多
Product family(PF) is the most important part of product platform. A new method is proposed to mine PF based on multi-space product data in PLM database. Product structure tree(PST) and bill of material(BOM) are used ...Product family(PF) is the most important part of product platform. A new method is proposed to mine PF based on multi-space product data in PLM database. Product structure tree(PST) and bill of material(BOM) are used as the data source. A PF can be obtained by mining physics space, logic space and attribute space of product data. In this work, firstly, a PLM database is described, consisting of data organization form, data structure, and data characteristics. Then the PF mining method introduces the sequence alignment techniques used in bio-informatics, which mainly includes data pre-processing, regularization, mining algorithm and cluster analysis. Finally, the feasibility and effectiveness of the proposed method are verified by a case study of high and middle pressure valve, demonstrating a feasible method to obtain PF from PLM database.展开更多
This essay studies various family-related factors that influence a child’s academic achievement in school in P.R. China. The factors are illustrated under three categories: the family’s social class and education, e...This essay studies various family-related factors that influence a child’s academic achievement in school in P.R. China. The factors are illustrated under three categories: the family’s social class and education, early home environment and family material condition. The author discusses the relevant factors by way of relating the knowledge of social environment to his own experiences as a student and then teacher in China. The essay closes with a discussion directing the public’s attention to the concern about home-related factors influencing a child’s academic achievements in school.展开更多
Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However...Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However,there is no comprehensive characterization of this gene family in cotton.In this study,32,16,and 18 BLH genes were identified from the G.hirsutum,G.arboreum,and G.raimondii genome,respectively.In addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH genes.Results:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis.Among them,25 members were assigned to 15 chromosomes.Furthermore,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in G.hirsutum.Expression profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation period.The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not auxin.Exogenous application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 transcripts.Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.Conclusions:The BLH gene family plays a very important role in many biological processes during plant growth and development.This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.展开更多
Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previou...Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in cotton.Results: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) analysis.Conclusions: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to biotic and abiotic resistance for the improvement in cotton.展开更多
Under the pressure of marketization and urbanization,rural family reproduction mode has transformed from simple reproduction to expanded family reproduction,thus changing the family structure and family relationship.U...Under the pressure of marketization and urbanization,rural family reproduction mode has transformed from simple reproduction to expanded family reproduction,thus changing the family structure and family relationship.Under the mode of expanded family reproduction,family relationship is a highly rational and deeply mobilized interaction pattern.And the family element configuration profoundly reflects the practical form of family relationship.Under the mode of expanded family reproduction,the changes in rural family relations are mainly reflected in three aspects:the decline of the paternal authority and the increasing family status of the offspring;the increasing status of women along with a decisive role in family decision-making;the more independent,equal,competitive and rational relationship among brothers.These changes bring forward new requirements on rural governance system,which requires improvements on social security and public culture.展开更多
Type 2 diabetes and obesity in young adults青年2型糖尿病和肥胖·Glucose-lowering medicines for type 2 diabetes2型糖尿病降血糖药物·The introduction of insulin in type 2 diabetes mellitus2型糖尿病胰岛素介绍...Type 2 diabetes and obesity in young adults青年2型糖尿病和肥胖·Glucose-lowering medicines for type 2 diabetes2型糖尿病降血糖药物·The introduction of insulin in type 2 diabetes mellitus2型糖尿病胰岛素介绍·Continuous glucose monitoring and pumps持续的血糖监测和抽送·Diet and diabetes饮食和糖尿病·Residential aged care facility residents:training issues for Australian general展开更多
General practitioners and general practice registrars：a comparison of clinical activity执业全科医生与全科培训生：临床活动比较General practice registrar education beyond the practice：the public health role of general...General practitioners and general practice registrars：a comparison of clinical activity执业全科医生与全科培训生：临床活动比较General practice registrar education beyond the practice：the public health role of general practitioners全科培训生在行医之外的教育：全科医生的公众健康角色·Editorial：medical educators：the unsung heroes of general practice？社评：医疗教育者——全科医疗的幕后英雄，展开更多
Musculoskeletal injury in Australian general practice：2000to 20152000—2015年澳大利亚全科医生对肌肉骨骼损伤的治疗Paediatric sports injuries小儿运动损伤Concussive head injury in children and
Background and Objective Desminopathy is a largely heterogeneous group of conditions involving inherited or sporadic myofibrillar myopathy.In terms of its mode of inheritance,the autosomal dominant form is predominant...Background and Objective Desminopathy is a largely heterogeneous group of conditions involving inherited or sporadic myofibrillar myopathy.In terms of its mode of inheritance,the autosomal dominant form is predominant.Desmin gene(DES)mutations play a critical role among the pathogenic inherited factors associated with desminopathy.Desminopathy involves various phenotypes,mainly including different cardiomyopathies,skeletal myopathy and arrhythmia.The purposes of this study are characterization of a novel phenotype and identification of a DES splicing mutation in a Chinese family with desminnopathy.展开更多
General practice encounters with patients living in residential aged care facilities全科医学应用于居住在养老院的患者·Models of general practitioner services in residential aged care facilities养老院中的全科医学...General practice encounters with patients living in residential aged care facilities全科医学应用于居住在养老院的患者·Models of general practitioner services in residential aged care facilities养老院中的全科医学服务模型·Minimising psychotropic use for behavioural disturbance in residential aged展开更多
1.Actions speak louder than louds,but words are a start.(行胜于言,但言语,才是开始.)简介:本文为世界家庭医生组织(WONCA)现任主席Amanda Howe教授接受Family Medicine and Community Health(FMCH)的邀请,为2018年10月在韩国首尔...1.Actions speak louder than louds,but words are a start.(行胜于言,但言语,才是开始.)简介:本文为世界家庭医生组织(WONCA)现任主席Amanda Howe教授接受Family Medicine and Community Health(FMCH)的邀请,为2018年10月在韩国首尔举办的WONCA第22届世界会议中代表全世界全科医生发表的对《首尔宣言》的准官方解读以及行动呼吁。在全文的最后,Amanda教授呼吁全世界的全科医生团结起来,为帮助各国实现全民健康覆盖及加强初级健康保健而共同行动。展开更多
Background and Objective Marfan syndrome,a variable and heritable disorder of fibrous connective tissue,characterized by affecting skeletal,ocular and cardiovascular systems.With the research advancement of genetic me...Background and Objective Marfan syndrome,a variable and heritable disorder of fibrous connective tissue,characterized by affecting skeletal,ocular and cardiovascular systems.With the research advancement of genetic mechanism,the diagnosis of Marfan syndrome,based on clinical manifestations and genetic evidence,is more accurate.The aim of this study is identification of genetic pathogenesis in a Chinese family.展开更多
Many products always operate under various complex environment conditions. To describe the dynamic influence of environment factors on their reliability, a method of reliability sensitivity analysis is proposed. In th...Many products always operate under various complex environment conditions. To describe the dynamic influence of environment factors on their reliability, a method of reliability sensitivity analysis is proposed. In this method, the location parameter is assumed as a function of relevant environment variables while the scale parameter is assumed as an unknown positive constant. Then, the location parameter function is constructed by using the method of radial basis function. Using the varied environment test data, the log-likelihood function is transformed to a generalized linear expression by describing the indicator as Poisson variable. With the generalized linear model, the maximum likelihood estimations of the model coefficients are obtained. With the reliability model, the reliability sensitivity is obtained. An instance analysis shows that the method is feasible to analyze the dynamic variety characters of reliability along with environment factors and is straightforward for engineering application.展开更多
文摘植物硝酸盐转运蛋白(Nitrate transporter,NRT)可有效转运NO-3,提升氮素利用效率。为了解析草地早熟禾(Poa pratensis)适应不同浓度及不同形态氮素、干旱胁迫机理,本研究以草地早熟禾为试材,对NRT1/PTR FAMILY 8.3基因进行克隆、生物信息学分析,并分析了不同浓度及不同形态氮素以及干旱下该基因表达情况。研究结果:草地早熟禾NRT1/PTR FAMILY 8.3基因包含典型的主要协同转运蛋白超家族(Major facilitator superfamily,MFS)结构域,与二穗短柄草(Brachypodium distachyon)高度同源;荧光定量PCR分析表明,该基因根部、叶部的表达量显著高于茎部;氮浓度为7.5 mM时,不同形态氮素诱导下,NaNO3处理组有利于该基因表达;无氮、高氮组(0,15 mM NaNO3)基因的表达量显著高于适氮组(7.5 mM NaNO3);干旱胁迫可促进其表达。研究结果为探究NRT1/PTR FAMILY 8.3在不同氮素环境中的调节机制,培育氮素利用率高的优质草种提供理论基础。
文摘慢性病患者的长期管理是基层医疗的核心任务之一,患者的规律随访(TR)与疾病预后密切相关。近期Annals of Family Medicine刊发了一篇文章《Teamwork among Primary Care Staff to Achieve Regular Follow-Up of Chronic Patients》,该文章指出慢性病患者的TR面临多重挑战,既往研究多聚焦于患者个体因素,而忽视了医疗团队动态对随访率的潜在影响。
文摘Creutzfeldt-Jakob disease(CJD)is a rare neurodegenerative disorder characterized by abnormalities in the prion protein(PrP),the most common form of human prion disease.Although Genome-Wide Association Studies(GWAS)have identified numerous risk genes for CJD,the mechanisms underlying these risk loci remain poorly understood.This study aims to elucidate novel genetically prioritized candidate proteins associated with CJD in the human brain through an integrative analytical pipeline.Utilizing datasets from Protein Quantitative Trait Loci(pQTL)(NpQTL1=152,NpQTL2=376),expression QTL(eQTL)(N=452),and the CJD GWAS(NCJD=4110,NControls=13569),we implemented a systematic analytical pipeline.This pipeline included Proteome-Wide Association Study(PWAS),Mendelian randomization(MR),Bayesian colocalization,and Transcriptome-Wide Association Study(TWAS)to identify novel genetically prioritized candidate proteins implicated in CJD pathogenesis within the brain.Through PWAS,we identified that the altered abundance of six brain proteins was significantly associated with CJD.Two genes,STX6 and PDIA4,were established as lead causal genes for CJD,supported by robust evidence(False Discovery Rate<0.05 in MR analysis;PP4/(PP3+PP4)≥0.75 in Bayesian colocalization).Specifically,elevated levels of STX6 and PDIA4 were associated with an increased risk of CJD.Additionally,TWAS demonstrated that STX6 and PDIA4 were associated with CJD at the transcriptional level.
文摘The Myb family of proteins is a group offunctionally diverse transcriptional activatorsfound in both plants and animals that ischaracterized by a conserved DNA bindingdomain of approximately 50 amino acids.Inplants,Myb proteins are involved in control
基金Supported by Heilongjiang Postdoctoral Fund(LBH-Z12045)Northeast Agricultural University Innovation Foundation For Postgraduate(yjscx14053)
文摘MicroRNA（miRNA） is a class of important regulating non-coding small molecular RNA. The gma-MIR166 gene family consists of 21 members and their expression patterns diversify widely. It is important to analyze the evolution of gma-MIR166 gene family in order to understand the evolutionary mechanisms of miRNAs in soybean. In this study, we implemented soybean wide genome block analysis, the molecular phylogeny of gma-MIR166 and block analysis of gma-MIR166 family. The results showed that both chromosome big segmental duplications and tandem duplications were main reasons contributed to the expanding of gmaMIR166 gene family. These findings suggested that gma-MIR166 gene family might originate from one or two ancient miRNA genes. The results of research provided a support for evolutionary study of miRNAs in soybean and related species in Fabaceae.
基金funded by the National Natural Science Foundation of China(31530053/31621005)the National Key R&D Program(2016YFD0101401/2017YFD0101601)
文摘Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to damage in cotton growth and a decrease in yields.Hyperosmolality-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration.Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants.However,the molecular nature of the corresponding calcium ion channels remains unclearly.In this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse genetics.Result:A total of 35,21 and 22 OSCA genes were identified in G.hirsutum,G.arboreum,and G.raimondii genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic relationship.Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) domain.Moreover,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress.Furthermore,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants.Conclusion:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton.These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes,thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions.
基金Project(51275362)supported by the National Natural Science Foundation of ChinaProject(2014ZX04015021)supported by National Science and Technology Major Project,China
文摘Product family(PF) is the most important part of product platform. A new method is proposed to mine PF based on multi-space product data in PLM database. Product structure tree(PST) and bill of material(BOM) are used as the data source. A PF can be obtained by mining physics space, logic space and attribute space of product data. In this work, firstly, a PLM database is described, consisting of data organization form, data structure, and data characteristics. Then the PF mining method introduces the sequence alignment techniques used in bio-informatics, which mainly includes data pre-processing, regularization, mining algorithm and cluster analysis. Finally, the feasibility and effectiveness of the proposed method are verified by a case study of high and middle pressure valve, demonstrating a feasible method to obtain PF from PLM database.
文摘This essay studies various family-related factors that influence a child’s academic achievement in school in P.R. China. The factors are illustrated under three categories: the family’s social class and education, early home environment and family material condition. The author discusses the relevant factors by way of relating the knowledge of social environment to his own experiences as a student and then teacher in China. The essay closes with a discussion directing the public’s attention to the concern about home-related factors influencing a child’s academic achievements in school.
基金the State Key Laboratory of Cotton Biology Open Fund(CB2019A03,2019A09 and CB2020A12)the National Natural Science Foundation of China(31872175)+2 种基金the Fundamental Research Funds for the Central Universities(GK202002005 and GK202001004)Natural Science Basic Research Plan in Shaanxi Province of China(2018JZ3006 and 2019JQ-062),Shaanxi Youth Entrusted Talents Program(20190205),Shaanxi Postdoctoral Project(2018BSHYDZZ76)Young Elite Scientists Sponsorship Program by CAST(2019-2021QNRC001).
文摘Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However,there is no comprehensive characterization of this gene family in cotton.In this study,32,16,and 18 BLH genes were identified from the G.hirsutum,G.arboreum,and G.raimondii genome,respectively.In addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH genes.Results:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis.Among them,25 members were assigned to 15 chromosomes.Furthermore,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in G.hirsutum.Expression profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation period.The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not auxin.Exogenous application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 transcripts.Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.Conclusions:The BLH gene family plays a very important role in many biological processes during plant growth and development.This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.
基金supported by China Agriculture Research System of MOF and MOARAAgricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Science
文摘Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in cotton.Results: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) analysis.Conclusions: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to biotic and abiotic resistance for the improvement in cotton.
基金National Social Science Fund Project 2016(16BKS047)
文摘Under the pressure of marketization and urbanization,rural family reproduction mode has transformed from simple reproduction to expanded family reproduction,thus changing the family structure and family relationship.Under the mode of expanded family reproduction,family relationship is a highly rational and deeply mobilized interaction pattern.And the family element configuration profoundly reflects the practical form of family relationship.Under the mode of expanded family reproduction,the changes in rural family relations are mainly reflected in three aspects:the decline of the paternal authority and the increasing family status of the offspring;the increasing status of women along with a decisive role in family decision-making;the more independent,equal,competitive and rational relationship among brothers.These changes bring forward new requirements on rural governance system,which requires improvements on social security and public culture.
文摘Type 2 diabetes and obesity in young adults青年2型糖尿病和肥胖·Glucose-lowering medicines for type 2 diabetes2型糖尿病降血糖药物·The introduction of insulin in type 2 diabetes mellitus2型糖尿病胰岛素介绍·Continuous glucose monitoring and pumps持续的血糖监测和抽送·Diet and diabetes饮食和糖尿病·Residential aged care facility residents:training issues for Australian general
文摘General practitioners and general practice registrars：a comparison of clinical activity执业全科医生与全科培训生：临床活动比较General practice registrar education beyond the practice：the public health role of general practitioners全科培训生在行医之外的教育：全科医生的公众健康角色·Editorial：medical educators：the unsung heroes of general practice？社评：医疗教育者——全科医疗的幕后英雄，
文摘Musculoskeletal injury in Australian general practice：2000to 20152000—2015年澳大利亚全科医生对肌肉骨骼损伤的治疗Paediatric sports injuries小儿运动损伤Concussive head injury in children and
文摘Background and Objective Desminopathy is a largely heterogeneous group of conditions involving inherited or sporadic myofibrillar myopathy.In terms of its mode of inheritance,the autosomal dominant form is predominant.Desmin gene(DES)mutations play a critical role among the pathogenic inherited factors associated with desminopathy.Desminopathy involves various phenotypes,mainly including different cardiomyopathies,skeletal myopathy and arrhythmia.The purposes of this study are characterization of a novel phenotype and identification of a DES splicing mutation in a Chinese family with desminnopathy.
文摘General practice encounters with patients living in residential aged care facilities全科医学应用于居住在养老院的患者·Models of general practitioner services in residential aged care facilities养老院中的全科医学服务模型·Minimising psychotropic use for behavioural disturbance in residential aged
文摘1.Actions speak louder than louds,but words are a start.(行胜于言,但言语,才是开始.)简介:本文为世界家庭医生组织(WONCA)现任主席Amanda Howe教授接受Family Medicine and Community Health(FMCH)的邀请,为2018年10月在韩国首尔举办的WONCA第22届世界会议中代表全世界全科医生发表的对《首尔宣言》的准官方解读以及行动呼吁。在全文的最后,Amanda教授呼吁全世界的全科医生团结起来,为帮助各国实现全民健康覆盖及加强初级健康保健而共同行动。
文摘Background and Objective Marfan syndrome,a variable and heritable disorder of fibrous connective tissue,characterized by affecting skeletal,ocular and cardiovascular systems.With the research advancement of genetic mechanism,the diagnosis of Marfan syndrome,based on clinical manifestations and genetic evidence,is more accurate.The aim of this study is identification of genetic pathogenesis in a Chinese family.
文摘Many products always operate under various complex environment conditions. To describe the dynamic influence of environment factors on their reliability, a method of reliability sensitivity analysis is proposed. In this method, the location parameter is assumed as a function of relevant environment variables while the scale parameter is assumed as an unknown positive constant. Then, the location parameter function is constructed by using the method of radial basis function. Using the varied environment test data, the log-likelihood function is transformed to a generalized linear expression by describing the indicator as Poisson variable. With the generalized linear model, the maximum likelihood estimations of the model coefficients are obtained. With the reliability model, the reliability sensitivity is obtained. An instance analysis shows that the method is feasible to analyze the dynamic variety characters of reliability along with environment factors and is straightforward for engineering application.
