Proteome analysis of embryo and endosperm of Givotia moluccana,a promising angiosperm tree species was carried out using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionization time o...Proteome analysis of embryo and endosperm of Givotia moluccana,a promising angiosperm tree species was carried out using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionization time of flight to identify differentially abundant proteins and their possible functions.The study revealed the presence of 492 protein spots in the embryo and 432 in the endosperm,and the relative abundance of 44 spots varied significantly between the two.Of the 21 functionally characterized protein spots,14.3%were involved in metabolism and energy,28.6%in protein destination and storage,14.3%in defense and stress and 19%in cell growth and division.The embryo-specific protein ATPdependent zinc metalloprotease FtsH is predicted to be involved in chloroplast biogenesis and the endospermspecific protein hydroxyacyl-ACP dehydratase in fatty acid synthesis.These results suggested that the seeds have proteins required for germination and for stress responses.展开更多
In this study,we revealed the differential proteins from the wheat endosperms using proteomic analysis and investigated their surface properties.The pattern of the polypeptides obtained from the Yangmai-15 and Yangmai...In this study,we revealed the differential proteins from the wheat endosperms using proteomic analysis and investigated their surface properties.The pattern of the polypeptides obtained from the Yangmai-15 and Yangmai-16 wheat varieties were compared using two-dimensional polyacrylamide gels.In addition,we compared the characteristics of the grain such as grain hardness,protein content,wet gluten,dough development time,dough stability,gliadin and glutenin contents between Yangmai-15 and Yangmai-16,and the results were significantly different.Notably,216 and 197 protein spots were separated from Yangmai-15 and Yangmai-16,respectively.The isoelectric points of the identified proteins ranged from 4 to 10 and the molecular weights of proteins varied from 10 to 100 kDa.Further,21 and 8 specific differential protein spots were identified fromthe flour of Yangmai-15 and Yangmai-16,respectively.The surface properties of identified peptides consisted of hydrophobic or hydrophilic residues,as well as randomly scattered residues.The proteomic analysis of the wheat endosperms provides a novel insight into the biochemical basis for the differences in physicochemical properties between the soft and hard wheat varieties.展开更多
基金Durgeshwar Pullaiahgari gratefully thanks the University Grants Commission(UGC)for providing a Below Basic Level(BBL)fellowship.We thank the Officials of the Mulugu Forest Research Station for allowing us to collect the seeds.Special thanks to Monica Kannan,Scientific Officer of the proteomics facility for mass spectral analysis.
文摘Proteome analysis of embryo and endosperm of Givotia moluccana,a promising angiosperm tree species was carried out using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionization time of flight to identify differentially abundant proteins and their possible functions.The study revealed the presence of 492 protein spots in the embryo and 432 in the endosperm,and the relative abundance of 44 spots varied significantly between the two.Of the 21 functionally characterized protein spots,14.3%were involved in metabolism and energy,28.6%in protein destination and storage,14.3%in defense and stress and 19%in cell growth and division.The embryo-specific protein ATPdependent zinc metalloprotease FtsH is predicted to be involved in chloroplast biogenesis and the endospermspecific protein hydroxyacyl-ACP dehydratase in fatty acid synthesis.These results suggested that the seeds have proteins required for germination and for stress responses.
基金the financial support of the National Natural Science Foundation of China(No.31771897,31871852,and 31772023)
文摘In this study,we revealed the differential proteins from the wheat endosperms using proteomic analysis and investigated their surface properties.The pattern of the polypeptides obtained from the Yangmai-15 and Yangmai-16 wheat varieties were compared using two-dimensional polyacrylamide gels.In addition,we compared the characteristics of the grain such as grain hardness,protein content,wet gluten,dough development time,dough stability,gliadin and glutenin contents between Yangmai-15 and Yangmai-16,and the results were significantly different.Notably,216 and 197 protein spots were separated from Yangmai-15 and Yangmai-16,respectively.The isoelectric points of the identified proteins ranged from 4 to 10 and the molecular weights of proteins varied from 10 to 100 kDa.Further,21 and 8 specific differential protein spots were identified fromthe flour of Yangmai-15 and Yangmai-16,respectively.The surface properties of identified peptides consisted of hydrophobic or hydrophilic residues,as well as randomly scattered residues.The proteomic analysis of the wheat endosperms provides a novel insight into the biochemical basis for the differences in physicochemical properties between the soft and hard wheat varieties.
