Escherichia coli O157 : H7 is a foodborne pathogen that poses a major threat to public health. Epidemiologic investigations have identified dairy cows, especially calves, are the principal reservoir of E. coli O157 : ...Escherichia coli O157 : H7 is a foodborne pathogen that poses a major threat to public health. Epidemiologic investigations have identified dairy cows, especially calves, are the principal reservoir of E. coli O157 : H7. In this study, based on the results, E. coli O157 : H7 was the main cause of E. coli disease outbreak in late October, 2015, and more than 90% of newborn calves died of serious diarrhea. Through further experiments, the drug sensitivity and resistance of the strain, the expression of the virulence gene and virulence pathogenicity were studied. E. coli O157 : H7 isolates were resistant to 12 antibiotics including penicillin, tetracycline and ampicillin, and were sensitive to eight antibiotics including cefoperazone, ceftazidime and amikacin. Resistance genes included tetB, strB, aadB, aphA, floR, TEM and virulence genes included stx1, eaeA and hlyA. Using specific pathogen free mice, the result showed that the isolate was pathogenic with a median lethal dose of 7.9×107 CFU · mL-1. This study described the pathogenesis and clinical manifestations of E. coli O157 : H7 infection. These results guided the use of antibiotics in prevent and control of bacterial infections in the future.展开更多
目的:分析江苏省不同地区和年代分离的EscherichiacoliO157:H7(E.coliO157:H7)之间的同源性。方法:用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分析法对113株E.coliO157:H7菌株进行分子分型,采用软件BioNumeric...目的:分析江苏省不同地区和年代分离的EscherichiacoliO157:H7(E.coliO157:H7)之间的同源性。方法:用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分析法对113株E.coliO157:H7菌株进行分子分型,采用软件BioNumerics Version4.0对分型数据进行处理和分析。结果:113株E.coliO157:H7共分37个型别,不同来源的菌株存在相同的基因型别,不同的地区和物种之间存在着相互传播。结论:AFLP可以用于对不同来源的E.coliO157:H7进行分子分型。展开更多
文摘Escherichia coli O157 : H7 is a foodborne pathogen that poses a major threat to public health. Epidemiologic investigations have identified dairy cows, especially calves, are the principal reservoir of E. coli O157 : H7. In this study, based on the results, E. coli O157 : H7 was the main cause of E. coli disease outbreak in late October, 2015, and more than 90% of newborn calves died of serious diarrhea. Through further experiments, the drug sensitivity and resistance of the strain, the expression of the virulence gene and virulence pathogenicity were studied. E. coli O157 : H7 isolates were resistant to 12 antibiotics including penicillin, tetracycline and ampicillin, and were sensitive to eight antibiotics including cefoperazone, ceftazidime and amikacin. Resistance genes included tetB, strB, aadB, aphA, floR, TEM and virulence genes included stx1, eaeA and hlyA. Using specific pathogen free mice, the result showed that the isolate was pathogenic with a median lethal dose of 7.9×107 CFU · mL-1. This study described the pathogenesis and clinical manifestations of E. coli O157 : H7 infection. These results guided the use of antibiotics in prevent and control of bacterial infections in the future.
文摘目的:分析江苏省不同地区和年代分离的EscherichiacoliO157:H7(E.coliO157:H7)之间的同源性。方法:用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分析法对113株E.coliO157:H7菌株进行分子分型,采用软件BioNumerics Version4.0对分型数据进行处理和分析。结果:113株E.coliO157:H7共分37个型别,不同来源的菌株存在相同的基因型别,不同的地区和物种之间存在着相互传播。结论:AFLP可以用于对不同来源的E.coliO157:H7进行分子分型。
