The experiment was conducted to determine effects of different dietary cation-anion difference（DCAD） in diets on ruminal fluid pH and fiber degradation in rumen of Laoshan dairy goats. A 4×4 latin square design...The experiment was conducted to determine effects of different dietary cation-anion difference（DCAD） in diets on ruminal fluid pH and fiber degradation in rumen of Laoshan dairy goats. A 4×4 latin square design was adopted. DCAD in different groups was 0, 50, 100, 200 mEq·kg^-1 of DM, respectively. The results of ruminal pH indicated that different DCAD could significantly influence the ruminal pH （P〈0.05） and ruminal fluid pH increased as DCAD increased from 0 to 200 mEq·kg^-1 of DM at different sampling time-points. There was no effect of DCAD on carboxymethyl cellulase in ruminal fluid at 4 h and 8 h postfeeding （P〉0.05）. Degradation ofNDF, ADF, CF peaked at a DCAD of 100 mEq·kg^-1 of DM. It could be concluded that DCAD of 100 mEq·kg^-1 of DM was advantage to non-pregnancy, non-lactication Laoshan dairy goat.展开更多
The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways wer...The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways were involved in the regulation of milk protein synthesis in the dairy goat mammary gland. Total 36 primiparous Guanzhong dairy goats were allotted in 12 groups according to their mammary development stages： days 90 and 150 of virgin, days 30, 90, and 150 of pregnancy, days 1, 10, 35, and 60 of lactation and days 3, 7, and 21 of involution （three animals per group）. Mammary tissue RNA was isolated for quantitative real- time RT-PCR of four casein genes alpha-s 1 casein （CSN 1S 1 ）, alpha-s2 casein （CSN 1 S2）, beta-casein （CSN2） and casein kappa （CSN3）, four whey protein genes lactoglobulin （LGB）, laetalbumin （LALBA）, laetofarrin （LTF）, and Whey acidic protein （WAP） and the genes which were potentially to regulate dairy goat milk protein synthesis at the level of transcription or translation [prolactin receptor （PRLR）, AKT1, signal transducers and activators of transcription 5 （STAT5）, E74-Like Factor 5 （ELF5）, eukaryotic translation initiation factor 4E binding protein 1 （EIF4E-BP1）, S6kinase （S6K） and caveolin 1]. The results showed that all genes were up-regulated in lactation period. The expressions of PRLR, AKT1, STAT5, ELF5, and S6K were similar to mRNA expressions of milk proteins. Our results indicated that milk protein synthesis in dairy goat mammary gland was possibly regulated by these genes.展开更多
RT-PCR and RACE techniques were used to clone 3＇ end real sequence of RPL11 gene from a goat. The sequences included some non-structural protein （3D） genes, and 3D gene immediately downstream 3＇ non-coding region ...RT-PCR and RACE techniques were used to clone 3＇ end real sequence of RPL11 gene from a goat. The sequences included some non-structural protein （3D） genes, and 3D gene immediately downstream 3＇ non-coding region （UTR） and poly （A） tail. The results showed that the length of specific amplified fragment was 566 bp （not including polyA tail）. RPL11 gene encoded 188 amino acids, amino acid sequence was conducted for homology analysis by NCBI BLAST tool. The highest homology was Mus （RPL11, mRNA homology 99%）, followed by Bos （RPL11, mRNA homology 96%）, and the next was Hs （RPL11, mRNA homology of 93%）.展开更多
基金Supported by Grant of Qingdao Agricultural University
文摘The experiment was conducted to determine effects of different dietary cation-anion difference（DCAD） in diets on ruminal fluid pH and fiber degradation in rumen of Laoshan dairy goats. A 4×4 latin square design was adopted. DCAD in different groups was 0, 50, 100, 200 mEq·kg^-1 of DM, respectively. The results of ruminal pH indicated that different DCAD could significantly influence the ruminal pH （P〈0.05） and ruminal fluid pH increased as DCAD increased from 0 to 200 mEq·kg^-1 of DM at different sampling time-points. There was no effect of DCAD on carboxymethyl cellulase in ruminal fluid at 4 h and 8 h postfeeding （P〉0.05）. Degradation ofNDF, ADF, CF peaked at a DCAD of 100 mEq·kg^-1 of DM. It could be concluded that DCAD of 100 mEq·kg^-1 of DM was advantage to non-pregnancy, non-lactication Laoshan dairy goat.
基金Supported by the National Basic Research Program of China(973 Program,2011CB100804)the National Natural Science Foundation of China(31101784)Funds for Young Researchers from Northeast Agricultural University(14QC43)
文摘The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways were involved in the regulation of milk protein synthesis in the dairy goat mammary gland. Total 36 primiparous Guanzhong dairy goats were allotted in 12 groups according to their mammary development stages： days 90 and 150 of virgin, days 30, 90, and 150 of pregnancy, days 1, 10, 35, and 60 of lactation and days 3, 7, and 21 of involution （three animals per group）. Mammary tissue RNA was isolated for quantitative real- time RT-PCR of four casein genes alpha-s 1 casein （CSN 1S 1 ）, alpha-s2 casein （CSN 1 S2）, beta-casein （CSN2） and casein kappa （CSN3）, four whey protein genes lactoglobulin （LGB）, laetalbumin （LALBA）, laetofarrin （LTF）, and Whey acidic protein （WAP） and the genes which were potentially to regulate dairy goat milk protein synthesis at the level of transcription or translation [prolactin receptor （PRLR）, AKT1, signal transducers and activators of transcription 5 （STAT5）, E74-Like Factor 5 （ELF5）, eukaryotic translation initiation factor 4E binding protein 1 （EIF4E-BP1）, S6kinase （S6K） and caveolin 1]. The results showed that all genes were up-regulated in lactation period. The expressions of PRLR, AKT1, STAT5, ELF5, and S6K were similar to mRNA expressions of milk proteins. Our results indicated that milk protein synthesis in dairy goat mammary gland was possibly regulated by these genes.
基金Supported by National High-tech Research and Development Program of China (863 Program) (2006AA10Z1A4)International Cooperation Projects of Heilongjiang Province (WB07A06)Innovation Team Project of Northeast Agricultural University (CXT005-1-1)
文摘RT-PCR and RACE techniques were used to clone 3＇ end real sequence of RPL11 gene from a goat. The sequences included some non-structural protein （3D） genes, and 3D gene immediately downstream 3＇ non-coding region （UTR） and poly （A） tail. The results showed that the length of specific amplified fragment was 566 bp （not including polyA tail）. RPL11 gene encoded 188 amino acids, amino acid sequence was conducted for homology analysis by NCBI BLAST tool. The highest homology was Mus （RPL11, mRNA homology 99%）, followed by Bos （RPL11, mRNA homology 96%）, and the next was Hs （RPL11, mRNA homology of 93%）.
