To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum ...To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.展开更多
文摘To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.
