OBJECTIVE The plant of Anchusa italicahas been traditionally used in Uighur medicine for the treatment of cardiovascular and cerebrovascular diseases in China.Our previous study showed that total flavonoids from Anchu...OBJECTIVE The plant of Anchusa italicahas been traditionally used in Uighur medicine for the treatment of cardiovascular and cerebrovascular diseases in China.Our previous study showed that total flavonoids from Anchusa italica(TFAI)exhibited potent cardioprotection on acute ischemia/reperfusion injured rats.This study was undertaken to investigate the effects of TFAI on chronic myocardial infarction in mice and the underlying mechanism.METHODS Total flavonoids were extracted from the whole herb of Anchusa italica and were characterized using HPLC-MS analysis.The left anterior descending branch of coronary artery was ligated to induce myocardial infarction in mice.After surgery,the mice were orally fed with TFAI at the doses of 10,30 and 50 mg·kg-1 body mass per day for a total of four weeks.Cardiac function and infarct size were measured,and the levels of inflammatory mediators were detected.Hematoxylin and eosin(HE)stain and Masson Trichrome stain were performed.The apoptotic factors such as Bax,Bcl-2 and cleaved caspase 3 as well as the key proteins in the PI3K/Akt/mTOR signaling pathway were examined by Western blotting.RESULTS The content of total flavonoids in TFAI was 56.2%.Four weeks following the MI surgery,TFAI enhanced the survival rate in post-MI mice.TFAI administration at the doses of 30 and 50 mg·kg-1 significantly reduced the infarct size and improved cardiac function indicated by elevated EF and FS.Assay of inflammation factors showed that the sera levels of TNF-α,IL-1β and IL-6 were significantly decreased by TFAI treatment as compared to the MI group.HE stain and Masson Trichrome stain demonstrated that TFAI suppressed myocyte hypertrophy and cardiac fibrosis indicated by decreased cross-section area and collagen volume.Western blot analysis showed that cleaved caspase 3 and Bax/Bcl-2 were signifi⁃cantly downregulated following TFAI treatment.Additionally,TFAI treatment significantly suppressed the activation of the PI3K/Akt/mTOR signaling pathway.CONCLUSION TFAI exerts a protective effect against chronic myocardial infarction and its beneficial effects on cardiac function and cardiac remodeling might be at least attributable to anti-inflammation and suppression of the PI3K/Akt/mTOR signaling pathway.展开更多
OBJECTIVE To evaluate the effects of flavonoids from Xindakang(Hippophae Fructus flavone)on myocardial systolic and diastolic functions of isolated frog hearts and explore the possible mechanism,and provide experiment...OBJECTIVE To evaluate the effects of flavonoids from Xindakang(Hippophae Fructus flavone)on myocardial systolic and diastolic functions of isolated frog hearts and explore the possible mechanism,and provide experimental basis for improving the effect and efficacy of Xindakang on cardiac function.METHODS The isolated frog heart perfusion specimens were prepared by Yagi′s method,and the effects of different concentrations of Xindakang on myocardial contractility(0.0125,0.025,0.05,0.1 and 0.2 g·L^(-1)),heart rate and cardiac output of isolated frog heart were studied.Acetylcholine,atropine and epinephrine were administered successively to analyze the effects of Xindakang on cardiac systolic function of isolated frogs under the action of different drugs,and compared with propranolol.The effect of extracellular calcium ion concentration on the action of Xindakang was studied by using low calcium concentration,high calcium concentration and normal Ren′s solution.To study the effect and possible mechanism of Xindakang on cardiac systolic function of frog.RESULTS The concentration of Xindakang in the range of 0.0125-0.1 g·L^(-1)could weaken the contractility of isolated frog heart and increase the concentration of Xindakang.The inhibitory effect of Xindakang on contractility of isolated frog heart was enhanced,and showed obvious dose-effect relationship.Cardiac output was significantly decreased by Xindakang(P<0.01),slow heart rate(P<0.05);M receptor blocker atropine could not antagonize the contractile effect of Xindakang,and Xindakang could not completely antagonize the contractile effect of adrenalin.Xindakang could inhibit the isolated frog heart in low calcium concentration,high calcium concentration and normal Ren′s solution,and increased with the increase of extracellular calcium concentration(P<0.01).CONCLUSION Xindakang has inhibitory effect on isolated frog heart,which may be achieved by blocking the calcium channel on myocardial cell membrane and reducing the calcium concentration in myocardial cells.展开更多
为探索有氧运动与心血管疾病患者心脏功能、脂质代谢和炎症关联性,通过检索PubMed、Embase、Scopus和中国知网(CNKI)数据库中有氧运动与心血管疾病患者心脏功能、脂质代谢和炎性因子影响的相关研究,利用RevMan5.4和R软件进行Meta和关联...为探索有氧运动与心血管疾病患者心脏功能、脂质代谢和炎症关联性,通过检索PubMed、Embase、Scopus和中国知网(CNKI)数据库中有氧运动与心血管疾病患者心脏功能、脂质代谢和炎性因子影响的相关研究,利用RevMan5.4和R软件进行Meta和关联性分析。结果表明:有氧运动显著降低了B型利钠肽(B-type natriuretic peptide,BNP)[标准化均数差(standardized mean difference,SMD)=-0.84,95%CI(-1.34,-0.34),P=0.001]、收缩压(SBP)[SMD=-0.55,95%CI(-0.86,-0.25),P=0.0004]和舒张压(DBP)[SMD=-0.99,95%CI(-1.67,-0.32),P=0.004]、LDL[SMD=-0.53,95%CI(-0.89,-0.18),P=0.003]和C-反应蛋白(CRP)[SMD=-0.53,95%CI(-0.90,-0.16),P=0.005]。CRP和HDL、LDL、DBP呈正相关,相关系数分别为0.35、0.26和0.28;CRP与SBP呈负相关,相关系数为-0.31。由此可见,心血管疾病患者参与有氧运动能够在一定程度改善心脏功能、脂质代谢和炎症因子水平,并且心脏功能和脂质代谢、炎症之间存在相关性。展开更多
目的:回顾性分析脊柱胸椎前凸患者及正常人群术前影像学及超声心动图资料,探讨并分析胸椎前凸与患者心脏结构及功能的关系。方法:收集并分析2013年1月~2023年12月期间胸椎前凸患者和正常人群的影像学及超声心动图资料。根据胸椎角度将...目的:回顾性分析脊柱胸椎前凸患者及正常人群术前影像学及超声心动图资料,探讨并分析胸椎前凸与患者心脏结构及功能的关系。方法:收集并分析2013年1月~2023年12月期间胸椎前凸患者和正常人群的影像学及超声心动图资料。根据胸椎角度将纳入患者分为两组,A组为胸椎前凸(TL)组(T5-T12≤0°),27例;B组为胸椎后凸(TK)减小组(0°<T5-T12≤20°),29例;另设正常TK(20°<T5-T12≤40°)的C组为对照组,29例。收集三组人群术前临床、影像学及超声心动图资料,包括一般资料[性别、年龄、身高、体重、体重指数(BMI)、体表面积(BSA)],X线片影像学资料(胸椎冠、矢状面Cobb角),顶椎区CT影像学资料[脊柱穿透指数(spinal penetration index volume,SPIV)、顶椎区心脏容积比(cardiac volume ratio in apical vertebra region,CVRA)],超声心动图资料[左心室舒张末期内径(LVDd)、右心室舒张末期内径(RVDd)、左心房内径(LAD)、右心房内径(RAD)、右心室流出道(RVOT)、主动脉内径(AO)、升主动脉内径(AAO)、主肺动脉内径(MPA)、舒张末期室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、射血分数(EF)、缩短分数(FS)、每搏输出量(SV)、心脏输出量(CO)、心脏指数(CI)、肺动脉收缩压(SPAP)]。采用SPSS 25.0统计分析软件对三组人群的上述资料进行统计、比较及相关性分析。结果:SPIV与矢状面Cobb角负相关,与冠状面Cobb角无相关性;SPIV与LVDd、RVDd、LAD、RAD、RVOT、AO、AAO、MPA、IVST、LVPWT呈负相关;SPIV与EF、FS、SV、CO、CI、SPAP无相关性;CVRA与矢状面Cobb角负相关,与冠状面Cobb角无相关性;CVRA与RVDd、IVST、LVPWT负相关;CVRA与LVDd、LAD、RAD、RVOT、AO、AAO、MPA之间无相关性;CVRA与EF、FS、SV、CO、CI、SPAP无相关性。结论:SPIV较CVRA更能反映胸椎前凸对心脏结构和功能的影响,胸椎后凸角度的减小可能加剧心脏血管负担。在胸椎前凸患者矫形手术过程中,由于全麻、俯卧位、胸骨及胸廓受压和肌松药物等因素,胸腔和心腔结构及功能参数可能发生显著变化,应特别关注气道阻塞和血流动力学不稳定的风险。展开更多
基金CAMS Innovation Fund for Medical Sciences(CIFMS)318(2016-I2M-3-007)National Natural Science Foundation of China(81673422and 81202538)
文摘OBJECTIVE The plant of Anchusa italicahas been traditionally used in Uighur medicine for the treatment of cardiovascular and cerebrovascular diseases in China.Our previous study showed that total flavonoids from Anchusa italica(TFAI)exhibited potent cardioprotection on acute ischemia/reperfusion injured rats.This study was undertaken to investigate the effects of TFAI on chronic myocardial infarction in mice and the underlying mechanism.METHODS Total flavonoids were extracted from the whole herb of Anchusa italica and were characterized using HPLC-MS analysis.The left anterior descending branch of coronary artery was ligated to induce myocardial infarction in mice.After surgery,the mice were orally fed with TFAI at the doses of 10,30 and 50 mg·kg-1 body mass per day for a total of four weeks.Cardiac function and infarct size were measured,and the levels of inflammatory mediators were detected.Hematoxylin and eosin(HE)stain and Masson Trichrome stain were performed.The apoptotic factors such as Bax,Bcl-2 and cleaved caspase 3 as well as the key proteins in the PI3K/Akt/mTOR signaling pathway were examined by Western blotting.RESULTS The content of total flavonoids in TFAI was 56.2%.Four weeks following the MI surgery,TFAI enhanced the survival rate in post-MI mice.TFAI administration at the doses of 30 and 50 mg·kg-1 significantly reduced the infarct size and improved cardiac function indicated by elevated EF and FS.Assay of inflammation factors showed that the sera levels of TNF-α,IL-1β and IL-6 were significantly decreased by TFAI treatment as compared to the MI group.HE stain and Masson Trichrome stain demonstrated that TFAI suppressed myocyte hypertrophy and cardiac fibrosis indicated by decreased cross-section area and collagen volume.Western blot analysis showed that cleaved caspase 3 and Bax/Bcl-2 were signifi⁃cantly downregulated following TFAI treatment.Additionally,TFAI treatment significantly suppressed the activation of the PI3K/Akt/mTOR signaling pathway.CONCLUSION TFAI exerts a protective effect against chronic myocardial infarction and its beneficial effects on cardiac function and cardiac remodeling might be at least attributable to anti-inflammation and suppression of the PI3K/Akt/mTOR signaling pathway.
基金Natural Science Foundation of Tibet Autonomous Region(2015ZR-13-16)。
文摘OBJECTIVE To evaluate the effects of flavonoids from Xindakang(Hippophae Fructus flavone)on myocardial systolic and diastolic functions of isolated frog hearts and explore the possible mechanism,and provide experimental basis for improving the effect and efficacy of Xindakang on cardiac function.METHODS The isolated frog heart perfusion specimens were prepared by Yagi′s method,and the effects of different concentrations of Xindakang on myocardial contractility(0.0125,0.025,0.05,0.1 and 0.2 g·L^(-1)),heart rate and cardiac output of isolated frog heart were studied.Acetylcholine,atropine and epinephrine were administered successively to analyze the effects of Xindakang on cardiac systolic function of isolated frogs under the action of different drugs,and compared with propranolol.The effect of extracellular calcium ion concentration on the action of Xindakang was studied by using low calcium concentration,high calcium concentration and normal Ren′s solution.To study the effect and possible mechanism of Xindakang on cardiac systolic function of frog.RESULTS The concentration of Xindakang in the range of 0.0125-0.1 g·L^(-1)could weaken the contractility of isolated frog heart and increase the concentration of Xindakang.The inhibitory effect of Xindakang on contractility of isolated frog heart was enhanced,and showed obvious dose-effect relationship.Cardiac output was significantly decreased by Xindakang(P<0.01),slow heart rate(P<0.05);M receptor blocker atropine could not antagonize the contractile effect of Xindakang,and Xindakang could not completely antagonize the contractile effect of adrenalin.Xindakang could inhibit the isolated frog heart in low calcium concentration,high calcium concentration and normal Ren′s solution,and increased with the increase of extracellular calcium concentration(P<0.01).CONCLUSION Xindakang has inhibitory effect on isolated frog heart,which may be achieved by blocking the calcium channel on myocardial cell membrane and reducing the calcium concentration in myocardial cells.
文摘为探索有氧运动与心血管疾病患者心脏功能、脂质代谢和炎症关联性,通过检索PubMed、Embase、Scopus和中国知网(CNKI)数据库中有氧运动与心血管疾病患者心脏功能、脂质代谢和炎性因子影响的相关研究,利用RevMan5.4和R软件进行Meta和关联性分析。结果表明:有氧运动显著降低了B型利钠肽(B-type natriuretic peptide,BNP)[标准化均数差(standardized mean difference,SMD)=-0.84,95%CI(-1.34,-0.34),P=0.001]、收缩压(SBP)[SMD=-0.55,95%CI(-0.86,-0.25),P=0.0004]和舒张压(DBP)[SMD=-0.99,95%CI(-1.67,-0.32),P=0.004]、LDL[SMD=-0.53,95%CI(-0.89,-0.18),P=0.003]和C-反应蛋白(CRP)[SMD=-0.53,95%CI(-0.90,-0.16),P=0.005]。CRP和HDL、LDL、DBP呈正相关,相关系数分别为0.35、0.26和0.28;CRP与SBP呈负相关,相关系数为-0.31。由此可见,心血管疾病患者参与有氧运动能够在一定程度改善心脏功能、脂质代谢和炎症因子水平,并且心脏功能和脂质代谢、炎症之间存在相关性。
文摘目的:回顾性分析脊柱胸椎前凸患者及正常人群术前影像学及超声心动图资料,探讨并分析胸椎前凸与患者心脏结构及功能的关系。方法:收集并分析2013年1月~2023年12月期间胸椎前凸患者和正常人群的影像学及超声心动图资料。根据胸椎角度将纳入患者分为两组,A组为胸椎前凸(TL)组(T5-T12≤0°),27例;B组为胸椎后凸(TK)减小组(0°<T5-T12≤20°),29例;另设正常TK(20°<T5-T12≤40°)的C组为对照组,29例。收集三组人群术前临床、影像学及超声心动图资料,包括一般资料[性别、年龄、身高、体重、体重指数(BMI)、体表面积(BSA)],X线片影像学资料(胸椎冠、矢状面Cobb角),顶椎区CT影像学资料[脊柱穿透指数(spinal penetration index volume,SPIV)、顶椎区心脏容积比(cardiac volume ratio in apical vertebra region,CVRA)],超声心动图资料[左心室舒张末期内径(LVDd)、右心室舒张末期内径(RVDd)、左心房内径(LAD)、右心房内径(RAD)、右心室流出道(RVOT)、主动脉内径(AO)、升主动脉内径(AAO)、主肺动脉内径(MPA)、舒张末期室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、射血分数(EF)、缩短分数(FS)、每搏输出量(SV)、心脏输出量(CO)、心脏指数(CI)、肺动脉收缩压(SPAP)]。采用SPSS 25.0统计分析软件对三组人群的上述资料进行统计、比较及相关性分析。结果:SPIV与矢状面Cobb角负相关,与冠状面Cobb角无相关性;SPIV与LVDd、RVDd、LAD、RAD、RVOT、AO、AAO、MPA、IVST、LVPWT呈负相关;SPIV与EF、FS、SV、CO、CI、SPAP无相关性;CVRA与矢状面Cobb角负相关,与冠状面Cobb角无相关性;CVRA与RVDd、IVST、LVPWT负相关;CVRA与LVDd、LAD、RAD、RVOT、AO、AAO、MPA之间无相关性;CVRA与EF、FS、SV、CO、CI、SPAP无相关性。结论:SPIV较CVRA更能反映胸椎前凸对心脏结构和功能的影响,胸椎后凸角度的减小可能加剧心脏血管负担。在胸椎前凸患者矫形手术过程中,由于全麻、俯卧位、胸骨及胸廓受压和肌松药物等因素,胸腔和心腔结构及功能参数可能发生显著变化,应特别关注气道阻塞和血流动力学不稳定的风险。
