The total RNA is extracted from leaf and bulb of Lycoris radiata(L’Hér.)Herb.by an improved method of CsCl density gradient centrifugation.The suitable amount of initial sample is 1.0 g.In order to remove impuri...The total RNA is extracted from leaf and bulb of Lycoris radiata(L’Hér.)Herb.by an improved method of CsCl density gradient centrifugation.The suitable amount of initial sample is 1.0 g.In order to remove impurities and purify the total RNA,the crude extract of RNA dissolved in lysis buffer is extracted with the mixed solution of chloroform and isoamyl alcohol(V ∶V,24 ∶1),then the total RNA is precipitated.The purity,integrity and quality of the total RNA are detected by ultraviolet spectrophotometer,agarose gel electrophoresis and RT-PCR method,respectively.The results show that polysaccharide can be removed effectively from the total RNA extract by the improved method of CsCl density gradient centrifugation.The purity andintegrityof the total RNA of L.radiata are better,and the ratio of A260/A280 is 1.85-2.00.It is concluded that the total RNA of L.radiata bulb extracted by the improved method can satisfy the demand of molecular biology experiments.展开更多
护理学是一门实践性很强的学科,具有直观性和规范性强的特点,实验中心建设也具有其独特性。CSCL(Computer Support for Collaborative Learning,计算机支持的协同学习)的目标是为学生的自主学习、交流互动学习提供支持,这样将CSCL引入...护理学是一门实践性很强的学科,具有直观性和规范性强的特点,实验中心建设也具有其独特性。CSCL(Computer Support for Collaborative Learning,计算机支持的协同学习)的目标是为学生的自主学习、交流互动学习提供支持,这样将CSCL引入到护理实验教学示范中心建设中来,既可以满足学生个性化学习、模拟学习和协作学习的需求,又能实现实验室教学资源共享,对学生提高护理课程理论知识的理解和掌握及实践创新能力的培养都起着非常重要的作用。展开更多
文摘The total RNA is extracted from leaf and bulb of Lycoris radiata(L’Hér.)Herb.by an improved method of CsCl density gradient centrifugation.The suitable amount of initial sample is 1.0 g.In order to remove impurities and purify the total RNA,the crude extract of RNA dissolved in lysis buffer is extracted with the mixed solution of chloroform and isoamyl alcohol(V ∶V,24 ∶1),then the total RNA is precipitated.The purity,integrity and quality of the total RNA are detected by ultraviolet spectrophotometer,agarose gel electrophoresis and RT-PCR method,respectively.The results show that polysaccharide can be removed effectively from the total RNA extract by the improved method of CsCl density gradient centrifugation.The purity andintegrityof the total RNA of L.radiata are better,and the ratio of A260/A280 is 1.85-2.00.It is concluded that the total RNA of L.radiata bulb extracted by the improved method can satisfy the demand of molecular biology experiments.
文摘护理学是一门实践性很强的学科,具有直观性和规范性强的特点,实验中心建设也具有其独特性。CSCL(Computer Support for Collaborative Learning,计算机支持的协同学习)的目标是为学生的自主学习、交流互动学习提供支持,这样将CSCL引入到护理实验教学示范中心建设中来,既可以满足学生个性化学习、模拟学习和协作学习的需求,又能实现实验室教学资源共享,对学生提高护理课程理论知识的理解和掌握及实践创新能力的培养都起着非常重要的作用。
