The ultrastructure of porcine kidney(PK)-15 cells was examined after lipofectamine-aided transfection of the molecular clone of the P1 agent.PK-15 cells transfected with the tandem dimer of the P1molecular DNA clone h...The ultrastructure of porcine kidney(PK)-15 cells was examined after lipofectamine-aided transfection of the molecular clone of the P1 agent.PK-15 cells transfected with the tandem dimer of the P1molecular DNA clone had numbers of intracytoplasmic inclusions,and a few cells had intranuclear inclusions.Intracytoplasmic inclusions were round to oval and 0.1-0.3μm in diameter,and intranuclear inclusions,which were more electron dense,were of two general types:the first were round and small(0.1μm approximately)and the second were hexagonal and larger(0.4-0.8μm in diameter).Cells transfected with the tandem dimer of the P1 molecular DNA clone tested positive for P1 DNA at passage 5.This is the first report that the P1 molecular clone has infectivity in vitro and it will provide fundamental materials for further study of the biological characterization of P1.展开更多
Combining the clonal selection mechanism of the immune system with the evolution equations of particle swarm optimization, an advanced algorithm was introduced for functions optimization. The advantages of this algori...Combining the clonal selection mechanism of the immune system with the evolution equations of particle swarm optimization, an advanced algorithm was introduced for functions optimization. The advantages of this algorithm lies in two aspects. Via immunity operation, the diversity of the antibodies was maintained, and the speed of convergent was improved by using particle swarm evolution equations. Simulation programme and three functions were used to check the effect of the algorithm. The advanced algorithm were compared with clonal selection algorithm and particle swarm algorithm. The results show that this advanced algorithm can converge to the global optimum at a great rate in a given range, the performance of optimization is improved effectively.展开更多
The objective of this study was to construct the infectious clone of encephalomyocarditis virus (EMCV) BJC3 strain.The genomic cDNA of the virus was amplified by three overlapping segments using RT-PCR,and cloned into...The objective of this study was to construct the infectious clone of encephalomyocarditis virus (EMCV) BJC3 strain.The genomic cDNA of the virus was amplified by three overlapping segments using RT-PCR,and cloned into low-copy plasmid pWSK29 to construct the full-length cDNA clone pWSKBJC3/ w.The pWSKBJC3/w was in vitro transcribed and transfected into BHK-21 cells to rescue the virus.The results showed that the full-length cDNA clone was infectious and the virus could be rescued in BHK-21 cells.The rescued virus designated RvBJC3W was identified by RT-PCR and indirect immunofluorescence assay(IFA).The rescued virus had similar growth characteristics to its parental virus BJC3 and retained pathogenicity for mice.Our results indicate that the first infectious cDNA clone of EMCV in China has been successfully established and provides an essential tool for investigating the molecular basis of pathogenicity of EMCV.展开更多
TDF1(transcription-drived fragment) was homologous to the predicted S. lycopersicum nonspecific lipid-transfer protein,nsLTP 2-like(91%), and it was significantly upregulated in response to C. fulvum(cladosporium fulv...TDF1(transcription-drived fragment) was homologous to the predicted S. lycopersicum nonspecific lipid-transfer protein,nsLTP 2-like(91%), and it was significantly upregulated in response to C. fulvum(cladosporium fulvum) infection in tomato plants.In this experiment, the full-length cDNA of nsLTP 2-like was cloned using RACE technology based on the sequence of TDF1(GenBank: JZ717725). A full-length, 625 bp(GenBank: KU366289), cDNA sequence, which with 98% similarity to nsLTP 2-like gene(GenBank: XM015233692) was obtained. This cDNA contains an ORF(open reading frame) with full-length of 345 bp, coding of 114 amino acids, including 12.3% Ala and Gly. Protein molecular weight was 11.51 ku, the isoelectric point(pI) was 8.99, and average overall hydrophilicity was 0.412, with one phosphorylation sites, belonging to volatile acidic nuclear protein. Secondary structure prediction showed that α-Helix accounts for 30.7%, extension chain for 12.28%, β-corner for 9.65%, and random coil for 47.37%. Through comparative analysis of the homology among species, it was found that the amino acid sequence of tomato nsLTP 2-like protein had a high similarity with other plants, and with a specific conserved sequence which might related features in nsLTP 2-like protein. It also be analyzed the gene expression pattern of tomato in different parts and under different stress conditions.The results showed that nsLTP 2-like gene was up-regulated in varying degrees, under the condition of cold stress, exogenous hormone spraying and cladosporium fulvum infection. Therefore, it was speculated that the gene played a role in response to abiotic and biotic stress in tomato.展开更多
Construction of infectious clones by full-length cDNA is basic and key for recovering RNA virus and is core of reverse genetics.In this article,basic consideration and key technology were viewed and factors affecting ...Construction of infectious clones by full-length cDNA is basic and key for recovering RNA virus and is core of reverse genetics.In this article,basic consideration and key technology were viewed and factors affecting infectivity of clones were also summarized.Some research advances were briefly introduced about positive-strand RNA viruses infectious clones.Finally,this article also reviewed the application of infectious clones.展开更多
The research screening of adoptive elite tea clones was conducted at NTRI, Mansehra during 2011-2012. Nine clones 101Aa, 105aa, 108aa, 561aa, ll7aa, 219ab, 470bb and 180bd were evaluated for seedling performance. Rand...The research screening of adoptive elite tea clones was conducted at NTRI, Mansehra during 2011-2012. Nine clones 101Aa, 105aa, 108aa, 561aa, ll7aa, 219ab, 470bb and 180bd were evaluated for seedling performance. Randomized complete block design was used with three replications. Data was recorded on various morphological characters after 8 months. The results showed that high survival percentage, shoot length, number of roots plant-1, number of leaves plant1 and root length were observed in clone 105aa. While the highest fresh weight and dry weight of leaves were observed in clones 117aa and 105aa. The clone 105aa was drought resistant, high survival percentage and root growth. On the basis of the results, clone 105aa was recommended for cultivation through cuttings in the hilly areas of Pakistan where unequal rainfall distribution was a major hitch.展开更多
The growth, morphology, and wind resistance of 21 Eucalyptus clones were investigated and analyzed in Leizhou Peninsula, Zhanjiang city. The results showed that coefficient of variation of each index phenotype varied ...The growth, morphology, and wind resistance of 21 Eucalyptus clones were investigated and analyzed in Leizhou Peninsula, Zhanjiang city. The results showed that coefficient of variation of each index phenotype varied from 15.21%-42.46%. There were significant differences among the clones in growth indexes. The top five clones with high individual volume were JJ144, 2#, 28#, 9#, and JJ197. The morphological indexes were significantly different among the clones. There were also significant differences among the clones in wind resistance index, and the clones with good wind resistance were 28#, JJ162, JJ195 and JJ215, their preservation rate were more than 80%. It was found that tree height and DBH significantly correlated to individual volume, and stem form was significantly related to branch. Using the principal component analysis, it was determined that JJ144, 2#, 28#, 9# and JJ162 were the excellent clones with good performance in the Leizhou area.展开更多
基金supported by the State Key Basic Research Project(973 project)of China(Grant No.2007CB116308)Planned Projects for Postdoctoral Research Funds of Jiangsu Province,China(Grant No.5910602)Postdoctoral Funds of Jiangsu Academy of Agricultural Sciences,China(Grant No.6510501)
文摘The ultrastructure of porcine kidney(PK)-15 cells was examined after lipofectamine-aided transfection of the molecular clone of the P1 agent.PK-15 cells transfected with the tandem dimer of the P1molecular DNA clone had numbers of intracytoplasmic inclusions,and a few cells had intranuclear inclusions.Intracytoplasmic inclusions were round to oval and 0.1-0.3μm in diameter,and intranuclear inclusions,which were more electron dense,were of two general types:the first were round and small(0.1μm approximately)and the second were hexagonal and larger(0.4-0.8μm in diameter).Cells transfected with the tandem dimer of the P1 molecular DNA clone tested positive for P1 DNA at passage 5.This is the first report that the P1 molecular clone has infectivity in vitro and it will provide fundamental materials for further study of the biological characterization of P1.
基金Project(A1420060159) supported by the National Basic Research of China projects(60234030, 60404021) supported by the National Natural Science Foundation of China
文摘Combining the clonal selection mechanism of the immune system with the evolution equations of particle swarm optimization, an advanced algorithm was introduced for functions optimization. The advantages of this algorithm lies in two aspects. Via immunity operation, the diversity of the antibodies was maintained, and the speed of convergent was improved by using particle swarm evolution equations. Simulation programme and three functions were used to check the effect of the algorithm. The advanced algorithm were compared with clonal selection algorithm and particle swarm algorithm. The results show that this advanced algorithm can converge to the global optimum at a great rate in a given range, the performance of optimization is improved effectively.
基金supported by the Key Project of National Natural Science Foundation of China(30530550)
文摘The objective of this study was to construct the infectious clone of encephalomyocarditis virus (EMCV) BJC3 strain.The genomic cDNA of the virus was amplified by three overlapping segments using RT-PCR,and cloned into low-copy plasmid pWSK29 to construct the full-length cDNA clone pWSKBJC3/ w.The pWSKBJC3/w was in vitro transcribed and transfected into BHK-21 cells to rescue the virus.The results showed that the full-length cDNA clone was infectious and the virus could be rescued in BHK-21 cells.The rescued virus designated RvBJC3W was identified by RT-PCR and indirect immunofluorescence assay(IFA).The rescued virus had similar growth characteristics to its parental virus BJC3 and retained pathogenicity for mice.Our results indicate that the first infectious cDNA clone of EMCV in China has been successfully established and provides an essential tool for investigating the molecular basis of pathogenicity of EMCV.
基金Supported by the National Key R&D Program of China(2017YFD0101900)China Agriculture Research System(CARS-23-A-16)the Science Foundation of Heilongjiang Province(C2017024)
文摘TDF1(transcription-drived fragment) was homologous to the predicted S. lycopersicum nonspecific lipid-transfer protein,nsLTP 2-like(91%), and it was significantly upregulated in response to C. fulvum(cladosporium fulvum) infection in tomato plants.In this experiment, the full-length cDNA of nsLTP 2-like was cloned using RACE technology based on the sequence of TDF1(GenBank: JZ717725). A full-length, 625 bp(GenBank: KU366289), cDNA sequence, which with 98% similarity to nsLTP 2-like gene(GenBank: XM015233692) was obtained. This cDNA contains an ORF(open reading frame) with full-length of 345 bp, coding of 114 amino acids, including 12.3% Ala and Gly. Protein molecular weight was 11.51 ku, the isoelectric point(pI) was 8.99, and average overall hydrophilicity was 0.412, with one phosphorylation sites, belonging to volatile acidic nuclear protein. Secondary structure prediction showed that α-Helix accounts for 30.7%, extension chain for 12.28%, β-corner for 9.65%, and random coil for 47.37%. Through comparative analysis of the homology among species, it was found that the amino acid sequence of tomato nsLTP 2-like protein had a high similarity with other plants, and with a specific conserved sequence which might related features in nsLTP 2-like protein. It also be analyzed the gene expression pattern of tomato in different parts and under different stress conditions.The results showed that nsLTP 2-like gene was up-regulated in varying degrees, under the condition of cold stress, exogenous hormone spraying and cladosporium fulvum infection. Therefore, it was speculated that the gene played a role in response to abiotic and biotic stress in tomato.
文摘Construction of infectious clones by full-length cDNA is basic and key for recovering RNA virus and is core of reverse genetics.In this article,basic consideration and key technology were viewed and factors affecting infectivity of clones were also summarized.Some research advances were briefly introduced about positive-strand RNA viruses infectious clones.Finally,this article also reviewed the application of infectious clones.
文摘The research screening of adoptive elite tea clones was conducted at NTRI, Mansehra during 2011-2012. Nine clones 101Aa, 105aa, 108aa, 561aa, ll7aa, 219ab, 470bb and 180bd were evaluated for seedling performance. Randomized complete block design was used with three replications. Data was recorded on various morphological characters after 8 months. The results showed that high survival percentage, shoot length, number of roots plant-1, number of leaves plant1 and root length were observed in clone 105aa. While the highest fresh weight and dry weight of leaves were observed in clones 117aa and 105aa. The clone 105aa was drought resistant, high survival percentage and root growth. On the basis of the results, clone 105aa was recommended for cultivation through cuttings in the hilly areas of Pakistan where unequal rainfall distribution was a major hitch.
基金National key research and development project(2016YFD0600500)Science and technology project of Guangdong province(2014B020202013)
文摘The growth, morphology, and wind resistance of 21 Eucalyptus clones were investigated and analyzed in Leizhou Peninsula, Zhanjiang city. The results showed that coefficient of variation of each index phenotype varied from 15.21%-42.46%. There were significant differences among the clones in growth indexes. The top five clones with high individual volume were JJ144, 2#, 28#, 9#, and JJ197. The morphological indexes were significantly different among the clones. There were also significant differences among the clones in wind resistance index, and the clones with good wind resistance were 28#, JJ162, JJ195 and JJ215, their preservation rate were more than 80%. It was found that tree height and DBH significantly correlated to individual volume, and stem form was significantly related to branch. Using the principal component analysis, it was determined that JJ144, 2#, 28#, 9# and JJ162 were the excellent clones with good performance in the Leizhou area.
