Granulocyte/macrophage colony-stimulatingfactor (GM-CSF) plays important roles in theregulation of hematogenesis and the immuneresponses. Vaccination with GM-CSF gene-modifiedtumor cells was demonstrated to be capable...Granulocyte/macrophage colony-stimulatingfactor (GM-CSF) plays important roles in theregulation of hematogenesis and the immuneresponses. Vaccination with GM-CSF gene-modifiedtumor cells was demonstrated to be capable ofinducing long-lasting, effective and specific antitumorimmune reaction. This protocol has entered展开更多
Patients with malignant glioma have a poorprognosis despite the combined use of surgery,irradiation, chemotherapy, and a variety ofimmunotherapies. These patient are known to showdecreased circulatory immune function ...Patients with malignant glioma have a poorprognosis despite the combined use of surgery,irradiation, chemotherapy, and a variety ofimmunotherapies. These patient are known to showdecreased circulatory immune function and suppression ofthe tumor-specific immune response. Interferon-γ(IFN-展开更多
The ultimate aim of cancer immunotherapy is theinduction of tumor-specific T-lymphocyte responseseffective in eradicating disseminated tumors. There aregenerally tumor infiltrating T lymphocytes in situ oftumor, but m...The ultimate aim of cancer immunotherapy is theinduction of tumor-specific T-lymphocyte responseseffective in eradicating disseminated tumors. There aregenerally tumor infiltrating T lymphocytes in situ oftumor, but most of which are inactive, one of the majorreasons of such Inactivation is that T lymphocytes areexposured to an antigen-specific TCR signal in theabsence of antigen-nonspecific or co-stimulatory signal.The co-stimulatory factors, B7-l and B7-2 on the surfaceof antigen presenting cells provide a key signal for thegeneration of T cell immunity. Sincc hepatoma展开更多
Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies...Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies is the introduction of herpes simplex virus type-1 thymidine kinase (HSV-tk) in the cells, followed bytreatment with the antiviral drug ganciclovir (GCV).展开更多
Several features of adenoviruses make them anattractive option as a vehicle to transfer genes intoprimary malignant neoplasms in vivo. These viruseshave low pathogenicity in humans and are notneurotoxic. In addition, ...Several features of adenoviruses make them anattractive option as a vehicle to transfer genes intoprimary malignant neoplasms in vivo. These viruseshave low pathogenicity in humans and are notneurotoxic. In addition, high titers of the virus can beachieved to allow higher levels of gene transfectionefficiency than the other vector systems. In vivotumorigenicity of G422 glioblastoma cells transfectedwith IL-2 and/or IL-3 genes decreased significantly inour privious report. In this study, recombinantadenoviruses were used to evaluate the therapeuticpotential of combined IL-2/IL-3 gene therapy in thetreatment of established subcutaneous tumor model ofG422 glioblastoma. Murine IL-2, IL-3 recombinantadenoviruses (2×10~8 pfu) were injected directly展开更多
Hepatocellular carcinoma (HCC) is one of the mostcommon cancers in the world, especially in East Asia.There is no standardized or effective strategy could beadapted routinely except of some early diagnosedpatients, an...Hepatocellular carcinoma (HCC) is one of the mostcommon cancers in the world, especially in East Asia.There is no standardized or effective strategy could beadapted routinely except of some early diagnosedpatients, and the prognosis is poor. In recent years, genetherapy has become a standard experimental approach fortreating cancers that have escaped conventionaltherapies. One such an approach is to confer the tumorcells with sensitivity to chemical reagents through展开更多
Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to...Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to treat melanoma, renal carcinoma,prostate carcinoma, colon carcinoma,展开更多
Interleukin-12, a heterodimer produced mainlyby antigen presenting cells (APCs), plays vital rolesin the induction of Thl-mediated immune responsesand has the potential to treat cancer and infectiousdiseases. Systemic...Interleukin-12, a heterodimer produced mainlyby antigen presenting cells (APCs), plays vital rolesin the induction of Thl-mediated immune responsesand has the potential to treat cancer and infectiousdiseases. Systemic administration of IL-12 wasobserved to be associated with severe toxicity. To takefull advantage of its immunoregulatory activities andminimize its toxicities, local administration of IL-12may be the ideal approach. Intratumoral injection展开更多
Tumor necrosis factor α (TNFα) and interleukin 2(IL-2) are cytokines that possess potent antitumor andimmunomodulatory activity such as enhancing monocyte-macrophage and neutrophil cytotoxic activities,increasing T ...Tumor necrosis factor α (TNFα) and interleukin 2(IL-2) are cytokines that possess potent antitumor andimmunomodulatory activity such as enhancing monocyte-macrophage and neutrophil cytotoxic activities,increasing T cell proliferation and IL-2 receptorexpression and augmenting cytotoxicity of cytotoxic Tlymphocyte. To investigate the therapeutic effect of thedirect cytokine gene transfer, recombinant展开更多
We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficienc...We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were展开更多
The progressive growth of tumors in the presence ofan intact immune system suggests that immunemechanisms may have failed to defend the host fromtumor cells. One therapeutic approach to cnhanceeffective immune respons...The progressive growth of tumors in the presence ofan intact immune system suggests that immunemechanisms may have failed to defend the host fromtumor cells. One therapeutic approach to cnhanceeffective immune responses is to introduce the immuno-regulatory genes that alter the local immunologicalmicroenvironment and increase immunogenecity of展开更多
In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Amo...In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.展开更多
To construct a recombinant adenovirus shuttle plasmid pDC315-H5HA-EGFP,the HA gene of A/Swine/Fujian/1/2001(H5N1) was amplified by RT-PCR and then inserted into adenovirus shuttle plasmid pDC315.A replication-defectiv...To construct a recombinant adenovirus shuttle plasmid pDC315-H5HA-EGFP,the HA gene of A/Swine/Fujian/1/2001(H5N1) was amplified by RT-PCR and then inserted into adenovirus shuttle plasmid pDC315.A replication-defective recombinant adenovirus expressing the HA gene(rAd-H5HA-EGFP) was generated by co-transfecting the recombinant shuttle plasmid pDC315-H5HA-EGFP and the genomic plasmid pBHGlox△E1,E3Cre in HEK293 cells.The recombinant adenovirus was confirmed by PCR,RT-PCR and Western blot assay.These results demonstrated that HA protein was properly expressed by the rAd-H5HA-EGFP in HEK293 cells and had natural biological activities.The TCID<sub>50</sub> of the rAd-H5HA- EGFP was assessed to be 2.26×10<sup>10</sup>/mL after propagation and purification.Immunization of BALB/ c mice indicated that rAd-H5HA-EGFP induced HI antibodies and protected mice from replication of the challenge virus in their lungs.展开更多
鸡包涵体肝炎（Inclusion Body Hepatitis,IBH）是由Ⅰ群禽腺病毒（Fowl adenovirusⅠ,FAV-Ⅰ）引起的一种急性传染病。该病最早发生在巴基斯坦卡拉奇市安卡拉地区,所以也称之为安卡拉病[1]。该病特征性病理变化为心包囊积液和肝炎。目...鸡包涵体肝炎（Inclusion Body Hepatitis,IBH）是由Ⅰ群禽腺病毒（Fowl adenovirusⅠ,FAV-Ⅰ）引起的一种急性传染病。该病最早发生在巴基斯坦卡拉奇市安卡拉地区,所以也称之为安卡拉病[1]。该病特征性病理变化为心包囊积液和肝炎。目前该病已在我国多地流行,可造成肉鸡、蛋鸡乃至水禽发病死亡,已严重影响养禽业健康发展[2-3]。展开更多
本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不...本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不同感染复数将Ad-eGFP、Ad-null或Ad-PDCD5转染人白血病细胞系,实时定量PCR检测PDCD5mRNA的相对表达水平;利用MTT法及Annexin-V-FITC/PI双染色流式细胞术观察依托泊甙对转染后K562细胞增殖与凋亡的影响。结果表明:Ad-eGFP腺病毒对白血病细胞系K562、Jurkat及CEM的转染效率可达60%-86%。Ad-PDCD5重组腺病毒能梯度增加K562细胞PDCD5 mRNA的相对表达水平,腺病毒介导的PDCD5基因转移促进依托泊甙诱导的K562细胞凋亡。结论:PDCD5重组腺病毒可能成为化疗药物的增敏剂。展开更多
文摘Granulocyte/macrophage colony-stimulatingfactor (GM-CSF) plays important roles in theregulation of hematogenesis and the immuneresponses. Vaccination with GM-CSF gene-modifiedtumor cells was demonstrated to be capable ofinducing long-lasting, effective and specific antitumorimmune reaction. This protocol has entered
文摘Patients with malignant glioma have a poorprognosis despite the combined use of surgery,irradiation, chemotherapy, and a variety ofimmunotherapies. These patient are known to showdecreased circulatory immune function and suppression ofthe tumor-specific immune response. Interferon-γ(IFN-
文摘The ultimate aim of cancer immunotherapy is theinduction of tumor-specific T-lymphocyte responseseffective in eradicating disseminated tumors. There aregenerally tumor infiltrating T lymphocytes in situ oftumor, but most of which are inactive, one of the majorreasons of such Inactivation is that T lymphocytes areexposured to an antigen-specific TCR signal in theabsence of antigen-nonspecific or co-stimulatory signal.The co-stimulatory factors, B7-l and B7-2 on the surfaceof antigen presenting cells provide a key signal for thegeneration of T cell immunity. Sincc hepatoma
文摘Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies is the introduction of herpes simplex virus type-1 thymidine kinase (HSV-tk) in the cells, followed bytreatment with the antiviral drug ganciclovir (GCV).
文摘Several features of adenoviruses make them anattractive option as a vehicle to transfer genes intoprimary malignant neoplasms in vivo. These viruseshave low pathogenicity in humans and are notneurotoxic. In addition, high titers of the virus can beachieved to allow higher levels of gene transfectionefficiency than the other vector systems. In vivotumorigenicity of G422 glioblastoma cells transfectedwith IL-2 and/or IL-3 genes decreased significantly inour privious report. In this study, recombinantadenoviruses were used to evaluate the therapeuticpotential of combined IL-2/IL-3 gene therapy in thetreatment of established subcutaneous tumor model ofG422 glioblastoma. Murine IL-2, IL-3 recombinantadenoviruses (2×10~8 pfu) were injected directly
文摘Hepatocellular carcinoma (HCC) is one of the mostcommon cancers in the world, especially in East Asia.There is no standardized or effective strategy could beadapted routinely except of some early diagnosedpatients, and the prognosis is poor. In recent years, genetherapy has become a standard experimental approach fortreating cancers that have escaped conventionaltherapies. One such an approach is to confer the tumorcells with sensitivity to chemical reagents through
文摘Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to treat melanoma, renal carcinoma,prostate carcinoma, colon carcinoma,
文摘Interleukin-12, a heterodimer produced mainlyby antigen presenting cells (APCs), plays vital rolesin the induction of Thl-mediated immune responsesand has the potential to treat cancer and infectiousdiseases. Systemic administration of IL-12 wasobserved to be associated with severe toxicity. To takefull advantage of its immunoregulatory activities andminimize its toxicities, local administration of IL-12may be the ideal approach. Intratumoral injection
文摘Tumor necrosis factor α (TNFα) and interleukin 2(IL-2) are cytokines that possess potent antitumor andimmunomodulatory activity such as enhancing monocyte-macrophage and neutrophil cytotoxic activities,increasing T cell proliferation and IL-2 receptorexpression and augmenting cytotoxicity of cytotoxic Tlymphocyte. To investigate the therapeutic effect of thedirect cytokine gene transfer, recombinant
文摘We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were
文摘The progressive growth of tumors in the presence ofan intact immune system suggests that immunemechanisms may have failed to defend the host fromtumor cells. One therapeutic approach to cnhanceeffective immune responses is to introduce the immuno-regulatory genes that alter the local immunologicalmicroenvironment and increase immunogenecity of
文摘In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.
基金supported by the Chinese National S&T Plan(2004BA519A55)Scientific Research Program of State Key Laboratory of Veterinary Biotechnology(NKLVBP200818)
文摘To construct a recombinant adenovirus shuttle plasmid pDC315-H5HA-EGFP,the HA gene of A/Swine/Fujian/1/2001(H5N1) was amplified by RT-PCR and then inserted into adenovirus shuttle plasmid pDC315.A replication-defective recombinant adenovirus expressing the HA gene(rAd-H5HA-EGFP) was generated by co-transfecting the recombinant shuttle plasmid pDC315-H5HA-EGFP and the genomic plasmid pBHGlox△E1,E3Cre in HEK293 cells.The recombinant adenovirus was confirmed by PCR,RT-PCR and Western blot assay.These results demonstrated that HA protein was properly expressed by the rAd-H5HA-EGFP in HEK293 cells and had natural biological activities.The TCID<sub>50</sub> of the rAd-H5HA- EGFP was assessed to be 2.26×10<sup>10</sup>/mL after propagation and purification.Immunization of BALB/ c mice indicated that rAd-H5HA-EGFP induced HI antibodies and protected mice from replication of the challenge virus in their lungs.
文摘鸡包涵体肝炎（Inclusion Body Hepatitis,IBH）是由Ⅰ群禽腺病毒（Fowl adenovirusⅠ,FAV-Ⅰ）引起的一种急性传染病。该病最早发生在巴基斯坦卡拉奇市安卡拉地区,所以也称之为安卡拉病[1]。该病特征性病理变化为心包囊积液和肝炎。目前该病已在我国多地流行,可造成肉鸡、蛋鸡乃至水禽发病死亡,已严重影响养禽业健康发展[2-3]。
文摘本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不同感染复数将Ad-eGFP、Ad-null或Ad-PDCD5转染人白血病细胞系,实时定量PCR检测PDCD5mRNA的相对表达水平;利用MTT法及Annexin-V-FITC/PI双染色流式细胞术观察依托泊甙对转染后K562细胞增殖与凋亡的影响。结果表明:Ad-eGFP腺病毒对白血病细胞系K562、Jurkat及CEM的转染效率可达60%-86%。Ad-PDCD5重组腺病毒能梯度增加K562细胞PDCD5 mRNA的相对表达水平,腺病毒介导的PDCD5基因转移促进依托泊甙诱导的K562细胞凋亡。结论:PDCD5重组腺病毒可能成为化疗药物的增敏剂。
