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Preparation of spherical HMX@PDA-based PBX by co-axial droplet microfluidic technology:Enhancing the interfacial effect and safety performance of composite microspheres 被引量:1
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作者 Yunyan Guo Yi Liu +6 位作者 Jiani Xie Jiawei Li Fan Wang Jinshan Lei Chongwei An Zhongliang Ma Bidong Wu 《Defence Technology(防务技术)》 2025年第3期73-83,共11页
Surface engineering plays a crucial role in improving the performance of high energy materials,and polydopamine(PDA)is widely used in the field of energetic materials for surface modification and functionalization.In ... Surface engineering plays a crucial role in improving the performance of high energy materials,and polydopamine(PDA)is widely used in the field of energetic materials for surface modification and functionalization.In order to obtain high-quality HMX@PDA-based PBX explosives with high sphericity and a narrow particle size distribution,composite microspheres were prepared using co-axial droplet microfluidic technology.The formation mechanism,thermal behavior,mechanical sensitivity,electrostatic spark sensitivity,compressive strength,and combustion performance of the microspheres were investigated.The results show that PDA can effectively enhance the interfacial interaction between the explosive particles and the binder under the synergistic effect of chemical bonds and the physical"mechanical interlocking"structure.Interface reinforcement causes the thermal decomposition temperature of the sample microspheres to move to a higher temperature,with the sensitivity to impact,friction,and electrostatic sparks(for S-1)increasing by 12.5%,31.3%,and 81.5%respectively,and the compressive strength also increased by 30.7%,effectively enhancing the safety performance of the microspheres.Therefore,this study provides an effective and universal strategy for preparing high-quality functional explosives,and also provides some reference for the safe use of energetic materials in practical applications. 展开更多
关键词 Droplet microfluidic technology Interfacial reinforcement Safety performance Surface modification POLYDOPAMINE HMX
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Advances in the Fabrication of Perovskite Solar Cells by Roll-to-Roll Technology
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作者 ZHAO Jiawei CHEN Haolin +1 位作者 LUO Ni LIU Zhenguo 《材料导报》 北大核心 2025年第1期98-114,共17页
In recent years,perovskite solar cells(PSCs)have garnered significant attention as a potential mainstream technology in the future photovol-taic(PV)market.This is primarily attributed to their salient advantages inclu... In recent years,perovskite solar cells(PSCs)have garnered significant attention as a potential mainstream technology in the future photovol-taic(PV)market.This is primarily attributed to their salient advantages including high efficiency,low cost,and ease of preparation.Nota-bly,the power conversion efficiency(PCE)of PSCs has experienced a remarkable increase from 3.8%in 2009 to over 26%at present.Conse-quently,the adoption of roll-to-roll(R2R)technology for PSCs is considered a crucial step towards their successful commercialization.This arti-de reviews the diverse substrates,scalable deposition techniques(such as solution-based knife-coating and spraying technology),and optimiza.tion procedures employed in recent years to enhance device performance within the R2R process.Additionally,novel perspectives are presented to enrich the existing knowledge in this field. 展开更多
关键词 perovskite solar cells roll-to-roll technology substrate scalable deposition technology performance optimization
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Adulteration Recognition Between Taoren and Xingren by Hyperspectral Non-destructive Technology with Mixed Metaheuristics RBF-SVM Model
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作者 Xu Hongzhao Zhao Qinghe +2 位作者 Liu Huaxi Zhang Zifang Fang Junlong 《Journal of Northeast Agricultural University(English Edition)》 2025年第2期66-81,共16页
Taoren and Xingren are commonly used herbs in East Asian medicine with different medication functions but huge economic differences,and there are cases of adulterated sales in market transactions.An effective adultera... Taoren and Xingren are commonly used herbs in East Asian medicine with different medication functions but huge economic differences,and there are cases of adulterated sales in market transactions.An effective adulteration recognition based on hyperspectral technology and machine learning was designed as a non-destructive testing method in this paper.A hyperspectral dataset comprising 500 Taoren and 500 Xingren samples was established;six feature selection methods were considered in the modeling of radial basis function-support vector machine(RBF-SVM),whose interaction between the two optimization methods was further researched.Two mixed metaheuristics modeling methods,Mixed-PSO and Mixed-SA,were designed,which fused both band selection and hyperparameter optimization from two-stage into one with detailed process analysis.The metrics of this mixed model were improved by comparing with traditional two-stage method.The accuracy of Mixed-PSO was 89.2%in five-floods crossvalidation that increased 4.818%than vanilla RBF-SVM;the accuracy of Mixed-SA was 88.7%which could reach the same as the traditional two-stage method,but it only relied on 48 crux bands in full 100 bands in RBF-SVM model fitting. 展开更多
关键词 hyperspectral technology adulteration recognition machine learning
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Complex adaptive system theory,agent-based modeling,and simulation in dominant technology formation 被引量:1
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作者 ZHANG Ruihan SUN Bing 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2024年第1期130-153,共24页
Dominant technology formation is the key for the hightech industry to“cross the chasm”and gain an established foothold in the market(and hence disrupt the regime).Therefore,a stimulus-response model is proposed to i... Dominant technology formation is the key for the hightech industry to“cross the chasm”and gain an established foothold in the market(and hence disrupt the regime).Therefore,a stimulus-response model is proposed to investigate the dominant technology by exploring its formation process and mechanism.Specifically,based on complex adaptive system theory and the basic stimulus-response model,we use a combination of agent-based modeling and system dynamics modeling to capture the interactions between dominant technology and the socio-technical landscape.The results indicate the following:(i)The dynamic interaction is“stimulus-reaction-selection”,which promotes the dominant technology’s formation.(ii)The dominant technology’s formation can be described as a dynamic process in which the adaptation intensity of technology standards increases continuously until it becomes the leading technology under the dual action of internal and external mechanisms.(iii)The dominant technology’s formation in the high-tech industry is influenced by learning ability,the number of adopting users and adaptability.Therein,a“critical scale”of learning ability exists to promote the formation of leading technology:a large number of adopting users can promote the dominant technology’s formation by influencing the adaptive response of technology standards to the socio-technical landscape and the choice of technology standards by the socio-technical landscape.There is a minimum threshold and a maximum threshold for the role of adaptability in the dominant technology’s formation.(iv)The socio-technical landscape can promote the leading technology’s shaping in the high-tech industry,and different elements have different effects.This study promotes research on the formation mechanism of dominant technology in the high-tech industry,presents new perspectives and methods for researchers,and provides essential enlightenment for managers to formulate technology strategies. 展开更多
关键词 complex adaptive system theory agent-based modeling and simulation dominant technology socio-technical landscape adaptation-choice
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基于Thermal Time模型预测高加索三叶草花序生长和花期发展
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作者 张慧敏 王明玖 +3 位作者 提忠慧 刘嘉伟 曹克璠 马一鸣 《草地学报》 北大核心 2025年第3期850-858,共9页
Thermal time模型认为植物需要高于基准温度(Base temperature,Tb)累积固定量的热效应(Thermal time,Tt)来完成特定的发育阶段,低于Tb或未达到固定量的Tt,不发生发育阶段的转化。本文在中国青藏高原、内蒙古高原、东北平原和新西兰坎特... Thermal time模型认为植物需要高于基准温度(Base temperature,Tb)累积固定量的热效应(Thermal time,Tt)来完成特定的发育阶段,低于Tb或未达到固定量的Tt,不发生发育阶段的转化。本文在中国青藏高原、内蒙古高原、东北平原和新西兰坎特伯雷平原设置5个试验区,以高加索三叶草(Trifolium ambiguum Bieb.)为研究对象,观测单个花序发育速率和种群开花进展速率。基于试验区温度,运用Thermal time模型预测花序生长和花期进展,旨在量化花序开放和花期推进所需的热量条件,以此指导高加索三叶草饲草利用和种子生产。结果表明,高加索三叶草从现蕾发育到最大花序尺寸约需在2℃以上累积25℃·d;从始花期到盛花期需在7.5℃以上累积635℃·d;从盛花期到结实期存在地形差异,高原地区需在8.7℃以上累积1813℃·d,平原地区需在3℃以上累积1290℃·d。在高原地区高加索三叶草更适合饲草利用,在平原地区有种子生产利用的潜质。 展开更多
关键词 高加索三叶草 温度 基温 花期 热效应
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A tensile wearable SHF antenna with efficient communication in defense beacon technology
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作者 Pooja Naresh Bhatt Rashmi Pandhare 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第11期198-210,共13页
The study projects a flexible and compact wearable pear-shaped Super High Frequency(SHF)antenna that can provide detailed location recognition and tracking applicable to defense beacon technology.This mini aperture wi... The study projects a flexible and compact wearable pear-shaped Super High Frequency(SHF)antenna that can provide detailed location recognition and tracking applicable to defense beacon technology.This mini aperture with electrical dimensions of 0.12λ_(0)×0.22λ_(0)×0.01λ_(0)attains a vast bandwidth over 3.1-34.5 GHz Super High Frequency(SHF)frequency band at S_(11)≤-10 dB,peak gain of 7.14 dBi and proportionately homogeneous radiation pattern.The fractional bandwidth(%BW)acquired is 168%that envelopes diversified frequency spectrum inclusive of X band specifically targeted to all kinds of defense and military operations.The proposed antenna can be worn on a soldier's uniform and hence the Specific Absorption Rate simulation is accomplished.The Peak SAR Value over 1 g of tissue is 1.48 W/kg and for 10 g of tissue is 0.27 W/kg well under the safety standards.The flexibility is proven by analyzing the full electromagnetic simulations for various bending conditions.Time response analysis is attained with its Fidelity Factor and Group Delay.Communication excellence is determined using Link Budget Analysis and it is seen that margin at 100 Mbps is 62 m and at 200 Mbps is 59 m.Prototype is fabricated along with experimental validation.All the results show harmony in shaping the antenna to provide critical situational awareness and data sharing capabilities required in defense beacon technology for location identification. 展开更多
关键词 COMPACT Defense beacon technology Link budget SAR time domain WEARABLE
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Precise time frequency synchronization technology for bistatic radar 被引量:6
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作者 Huang Yulin Yang Jianyu Wu Junjie Xiong Jintao 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2008年第5期929-933,共5页
A complete method of synchronization technology of bistatic radar using global position system (GPS) is presented. The pulse per second signal (1PPS) is elaborately modified to increase the time synchronization pr... A complete method of synchronization technology of bistatic radar using global position system (GPS) is presented. The pulse per second signal (1PPS) is elaborately modified to increase the time synchronization precision and keep loop locking. A very high time synchronization precision is achieved. Using the modified 1PPS to discipline the local OCXO, the reference frequency signal achieves both high long term stability (LTS) and short term stability (STS) properties. An algorithm, named phase abrupt change CFAR is presented to restrain the 1PPS phase abrupt change and keep loop locking. The experimental results indicate that this time and frequency synchronization method is effective and the time synchronization precision of the synchronization system can be improved from ±100 ns to ±25 ns. In addition, the phase noise is improved to 20 dB. 展开更多
关键词 bistatic radar GPS time synchronization frequency phase synchronization.
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Generic reconstruction technology based on RST for multivariate time series of complex process industries 被引量:1
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作者 孔玲爽 阳春华 +2 位作者 李建奇 朱红求 王雅琳 《Journal of Central South University》 SCIE EI CAS 2012年第5期1311-1316,共6页
In order to effectively analyse the multivariate time series data of complex process,a generic reconstruction technology based on reduction theory of rough sets was proposed,Firstly,the phase space of multivariate tim... In order to effectively analyse the multivariate time series data of complex process,a generic reconstruction technology based on reduction theory of rough sets was proposed,Firstly,the phase space of multivariate time series was originally reconstructed by a classical reconstruction technology.Then,the original decision-table of rough set theory was set up according to the embedding dimensions and time-delays of the original reconstruction phase space,and the rough set reduction was used to delete the redundant dimensions and irrelevant variables and to reconstruct the generic phase space,Finally,the input vectors for the prediction of multivariate time series were extracted according to generic reconstruction results to identify the parameters of prediction model.Verification results show that the developed reconstruction method leads to better generalization ability for the prediction model and it is feasible and worthwhile for application. 展开更多
关键词 complex process industry prediction model multivariate time series rough sets
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Application of Multimedia and Internet Technology in Learning Mould Design
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作者 Simon S P Shum 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期295-296,共2页
With the use of multimedia which combines the use o f text, sound, images, motion video and animation, it is more efficient for studen ts to learn mould design interactively. A program is created using several multi m... With the use of multimedia which combines the use o f text, sound, images, motion video and animation, it is more efficient for studen ts to learn mould design interactively. A program is created using several multi media software to simulate the mechanism of moulding processes in order to let s tudents understand the concept of mould design. In addition, students can even access the program through the Internet. Therefore, the software is defined as Multimedia and Internet Technology (MIT) program. The MIT program consists of four sections: (i) simulation of mould mechanisms, ( ii) cooling system, (iii) material information and (iv) games for tutorials. Sec tion One covers the basic operations of different types of moulds such as two-p late mould, three-plate mould, split mould, side-core mould and mould with und ercuts. Section Two introduces different types of cooling systems such as bubble r, baffle, cooling circuits, etc. Section Three provides some useful material in formation for mould design. Section Four contains games of matching mould compon ents, mould design problem finding and multiple choice questions to test student s how much they understand mould design concept. Multimedia is highly effective particularly in teaching and learning. It changes the nature of learning itself. It makes reading dynamic by giving words an impo rtant new dimension. It allows students to see, hear and do simultaneously, thus significantly reducing average learning time. Furthermore, through cooperative learning on Internet, students can access the program, share data or search info rmation anytime anywhere. Therefore, Multimedia and Internet Technology is one o f the vital aspects to speed up the realization of information age in society. 展开更多
关键词 Application of Multimedia and Internet technology in Learning Mould Design
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Impact time control guidance for moving-target considering velocity variation and field-of-view constraint
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作者 YANG Hao ZHANG Shifeng +1 位作者 BAI Xibin YANG Chengye 《Journal of Systems Engineering and Electronics》 2025年第2期552-568,共17页
In the existing impact time control guidance (ITCG) laws for moving-targets, the effects of time-varying velocity caused by aerodynamics and gravity cannot be effectively con-sidered. Therefore, an ITCG with field-of-... In the existing impact time control guidance (ITCG) laws for moving-targets, the effects of time-varying velocity caused by aerodynamics and gravity cannot be effectively con-sidered. Therefore, an ITCG with field-of-view (FOV) constraints based on biased proportional navigation guidance (PNG) is developed in this paper. The remaining flight time (time-to-go) estimation method is derived considering aerodynamic force and gravity. The number of differential equations is reduced and the integration step is increased by changing the integral variable, which makes it possible to obtain time-to-go through integration. An impact time controller with FOV constraints is proposed by analyzing the influence of the biased term on time-to-go and FOV constraint. Then, numerical simulations are performed to verify the correctness and superiority of the method. 展开更多
关键词 moving target time-varying velocity time-to-go esti-mation field-of-view(FOV)constraint flight time control.
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基于TimeGAN-Stacking的风电机组变桨系统故障诊断方法 被引量:1
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作者 潘美琪 贺兴 《太阳能学报》 北大核心 2025年第1期192-200,共9页
风电机组变桨系统的少量不均衡故障样本难以训练基于数据驱动的故障诊断模型,导致监测系统常常漏报或误报故障。针对上述问题,提出一种基于TimeGAN-Stacking的风电机组变桨系统故障诊断方法。在数据层面,由于原始样本类别不平衡,基于时... 风电机组变桨系统的少量不均衡故障样本难以训练基于数据驱动的故障诊断模型,导致监测系统常常漏报或误报故障。针对上述问题,提出一种基于TimeGAN-Stacking的风电机组变桨系统故障诊断方法。在数据层面,由于原始样本类别不平衡,基于时序生成对抗网络(TimeGAN)跟踪风电机组运行数据逐步概率分布的动态变化特征,同时优化生成样本的全局分布与局部分布,有效平衡且扩容风电机组多种故障综合样本集;在模型层面,建立Stacking集成模型,融合多个故障诊断器的优势,进一步提高故障诊断能力。最后,基于实际风场数据对所提方法进行测试,结果表明,所提出的TimeGAN-Stacking故障识别方法可有效诊断4种变桨故障。 展开更多
关键词 风电机组 数据挖掘 故障分析 深度学习 时序生成对抗网络 样本增强
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基于TL-TimeGAN的多维时间序列数据增强及其应用分析
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作者 智路平 汪万敏 《运筹与管理》 北大核心 2025年第5期177-184,I0060-I0064,共13页
针对部分场景下标签较少、样本不均衡的时序数据,为了更好的捕捉序列之间的逐步依赖关系,本文一方面使用具有因果关系属性的时域卷积网络构建生成对抗网络,另一方面使用长短期记忆网络构建嵌入网络和复现网络,以实现模型同时处理短期依... 针对部分场景下标签较少、样本不均衡的时序数据,为了更好的捕捉序列之间的逐步依赖关系,本文一方面使用具有因果关系属性的时域卷积网络构建生成对抗网络,另一方面使用长短期记忆网络构建嵌入网络和复现网络,以实现模型同时处理短期依存项和长期依存项,从而提出一种基于时域卷积网络和长短期记忆网络的时间序列生成对抗网络(A Time-series Generative Adversarial Network based on Temporal convolutional network and Long-short term memory network, TL-TimeGAN)。采用覆盖性、有用性和相似度检验的综合分析方法作为合成数据质量的评价指标,进一步全面地评价合成数据的覆盖性、预测程度和相似性。最终,基于以太坊欺诈检测数据集,使用Tabnet网络对扩增数据进行异常检测并获得局部特征重要性以及全局特征重要性,以增强扩增数据应用于实际工作的实践指导价值。 展开更多
关键词 时域卷积网络 长短期记忆网络 时间序列生成对抗网络 时序数据增强 多维时间序列
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基于拓展Volume-Time理论的串联空气间隙非同期击穿过程分析
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作者 姚晓飞 常晓雪 +5 位作者 丁健刚 陈佳莉 刘学 王建华 刘志远 耿英三 《高压电器》 北大核心 2025年第3期119-127,159,共10页
为了认识串联空气间隙的击穿过程,以球—球间隙为研究对象,设计了“非对称组合间隙”,即总间隙距离不变的条件下分别单独调整两个间隙的距离使上下间隙距离不一致。采用拓展Volume-Time理论计算了串联双间隙的U50%,并解释了雷电冲击电... 为了认识串联空气间隙的击穿过程,以球—球间隙为研究对象,设计了“非对称组合间隙”,即总间隙距离不变的条件下分别单独调整两个间隙的距离使上下间隙距离不一致。采用拓展Volume-Time理论计算了串联双间隙的U50%,并解释了雷电冲击电压下非对称组合空气间隙的非同期击穿物理过程。实验结果表明,上下间隙距离分布不均匀程度对串联空气间隙击穿电压和击穿延时的影响;不均匀程度较大时,较低电压下存在单个间隙放电、总体不击穿的现象,较高电压下两间隙将出现非同期击穿;不均匀程度较小时,两间隙同期击穿。为了解释这种现象,文中采用拓展Volume-Time理论,通过确定临界体积、临界面积等关键参数来计算串联双间隙的击穿电压。并把串联双空气间隙击穿的原因解释为,空气间隙电场的有效体积与有效面积超过临界值。因此,双间隙非同期击穿的产生是由于当一个间隙达到了击穿起始条件,而另一个间隙还未达到击穿起始条件。 展开更多
关键词 双断口开关 串联空气间隙 雷电冲击电压 非同期击穿特性 Volume-time理论
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《Agricultural Science & Technology》综合影响力分析
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作者 陈娟 张志转 +2 位作者 李杨 朱安 朱永和 《安徽农业科学》 CAS 北大核心 2010年第28期F0002-F0002,F0003,共2页
从引证指标、网络传播情况以及作者构成等方面阐述《Agricultural Science & Technology》改革后的综合影响力。
关键词 AGRICULTURAL SCIENCE & technology 引证指标 网络传播 作者构成
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英国国家临床技术研究院腹腔镜疝修补术技术指南(National Institute for Clinical Excellence,Technology Appraisal Guidance 83:Laparoscopic surgery for inguinal hernia repair) 被引量:16
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作者 储修峰 郭绍红 《中国微创外科杂志》 CSCD 2007年第12期1132-1134,共3页
关键词 腹腔镜疝修补术 TEP 开放手术 腹股沟疝修补术 National Institute for Clinical Excellence technology Appraisal Guidance 83 TAPP 腹腔镜手术 临床技术 英国 大不列颠及北爱尔兰联合王国 研究院
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设计未来技术发展蓝图的有效方法——Technology Roadmapping 被引量:6
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作者 王瑞祥 《科技进步与对策》 北大核心 2002年第7期117-119,共3页
Technology Roadmapping可译作“技术蓝图设计”,简称TRM。它是一种流行的技术规划方法,可在企业(或R&D组织)、产业两个不同层次上进行。其主要功能是从未来产品(或服务)的需求出发,由技术、市场等各方面的专家合作,选择适用技术并... Technology Roadmapping可译作“技术蓝图设计”,简称TRM。它是一种流行的技术规划方法,可在企业(或R&D组织)、产业两个不同层次上进行。其主要功能是从未来产品(或服务)的需求出发,由技术、市场等各方面的专家合作,选择适用技术并确定相应技术路线,描绘出未来技术发展蓝图(Technology Roadmaps)。 展开更多
关键词 设计 技术发展蓝图 技术预测 技术规划
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理解为残疾人学习的教育技术:福祉技术(Assistive Technology)——访谈约翰霍普金斯大学学者约翰·卡斯特兰尼 被引量:8
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作者 张宝辉 林苹苹 田党瑞 《现代远程教育研究》 CSSCI 2013年第4期19-24,共6页
随着技术的进步和社会的发展,为残疾人和弱势群体使用的教育技术——Assistive Technology也得到了较快的发展。访谈者参考日本学者后藤芳在其著作《福祉技术入门》中对辅助技术的翻译,在这里将Assistive Technology按照意译翻译为福祉... 随着技术的进步和社会的发展,为残疾人和弱势群体使用的教育技术——Assistive Technology也得到了较快的发展。访谈者参考日本学者后藤芳在其著作《福祉技术入门》中对辅助技术的翻译,在这里将Assistive Technology按照意译翻译为福祉技术。需要指出的是,有很多人将Assistive Technology翻译为辅助技术,为了能够体现新技术对残疾人和弱势群体的关爱和辅助,访谈者的翻译与此一致并考虑了技术使用目的与情境。访谈者通过面对面访谈约翰.霍普金斯大学的约翰.卡斯特兰尼副教授和之后的数次邮件和电话沟通,介绍了国际上福祉技术现状、相关研究、趋势、热点及相关政策;同时介绍了相关的学会、刊物、学者、流派、现状。我们希望本文能够引起更多中国教育技术及相关领域学者对福祉技术的关注、设计以及应用,关注和研究社会弱势群体教育问题。 展开更多
关键词 福祉技术 残疾人 国际现状 学者访谈 弱势群体学习
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Effect of Hot Processing Technology on Mechanical Properties and Structure of Interface of Ti/Steel Composite Sheet 被引量:6
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作者 关尚哲 刘润生 +1 位作者 范江峰 王小兵 《稀有金属快报》 CSCD 2005年第11期25-30,共6页
研究了热加工工艺对钛-钢复合板界面力学性能和显微组织的影响。测试了在A,B,C,D4种温度下热轧复合板界面的力学性能,用金相显微镜及扫描电镜观察了界面显微组织并分析了界面的成分。结果表明,在A,B2种温度下轧制的钛-钢复合板界... 研究了热加工工艺对钛-钢复合板界面力学性能和显微组织的影响。测试了在A,B,C,D4种温度下热轧复合板界面的力学性能,用金相显微镜及扫描电镜观察了界面显微组织并分析了界面的成分。结果表明,在A,B2种温度下轧制的钛-钢复合板界面机械性能良好,延伸率高,其剪切强度不但可保持坯料原有的水平,甚至还略有增加。在C,D2种温度下轧制的钛-钢复合板界面机械性能相对较低,延伸率较高,但剪切强度要比爆炸复合坯料低,尤其是D加热温度,轧制后界面剪切强度急剧下降。热轧的终轧温度也是影响钛-钢复合板界面结合性能的重要因素。在低于相转变温度的合适温区热轧,且终轧温度合适,获得的钛-钢复合板结合界面无爆炸波纹,没有污染,生产的脆性化合物极细小,组织类同于钛材完全退火的等轴组织。 展开更多
关键词 钛-钢复合板 热加工工艺 力学性能 金属间化合物
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4种植物源性成分多重real-time PCR检测方法的建立及其在食用淀粉中的应用 被引量:3
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作者 范维 高晓月 +4 位作者 董雨馨 刘虹宇 李贺楠 赵文涛 郭文萍 《食品科学》 EI CAS CSCD 北大核心 2024年第1期210-216,共7页
建立一种可同时快速检测红薯、木薯、马铃薯、玉米源性成分的多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法。分别以红薯g3pdh基因、木薯g3pdh基因、马铃薯UGPase基因、玉米zSSIIb基因为靶基因设计... 建立一种可同时快速检测红薯、木薯、马铃薯、玉米源性成分的多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法。分别以红薯g3pdh基因、木薯g3pdh基因、马铃薯UGPase基因、玉米zSSIIb基因为靶基因设计特异性引物和TaqMan探针,以18S rRNA基因为内参基因,建立多重real-time PCR方法,开展方法学验证,并对不同掺入比例模拟样品和实际淀粉样品进行检测。结果显示,该方法具有高通量、特异性强、灵敏度高等优点。与15种非目标源性均无交叉反应;对目标DNA的检测灵敏度可达到3×10^(-3) ng/μL,且具有良好的线性关系和扩增效率;对淀粉样品的检出限可达0.1%,对50份实际样品进行检测,结果与参比方法一致,说明建立的多重real-time PCR法可用于食用淀粉种类掺假鉴别检测。 展开更多
关键词 多重实时聚合酶链式反应 食用淀粉 木薯 红薯 马铃薯 玉米
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Incorporation of meat quality and use of ultrasound technology for swine genetic improvement programs 被引量:9
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作者 Tom J.BAAS 《华南农业大学学报》 CAS CSCD 北大核心 2005年第S1期88-98,共11页
Real-time ultrasound has been, and will continue to be, one of the most important tools in enhancing genetic improvement in the U.S. Recent advancements in the technology have allowed ultrasound to be used in evaluati... Real-time ultrasound has been, and will continue to be, one of the most important tools in enhancing genetic improvement in the U.S. Recent advancements in the technology have allowed ultrasound to be used in evaluating intramuscular fat in live animals. Together with other genetic improvement tools, ultrasound technology will offer seedstock producers the opportunity to select for improved IMF in potential breeding stock replacements and hence speed genetic progress for the improvement of this trait. After three generations of selection for IMF using real-time ultrasound in an Iowa State University study, the average EBV for select line pigs is 0.83% greater than for control line pigs. Selection for IMF has, however, resulted in slightly more backfat and less loin muscle area, and a trend toward more days to 114 kg in the select line compared to the control line. Carcass evaluation of a sample of pigs from each litter indicated a similar increase in IMF, increase in backfat, and reduction in loin muscle area for select line pigs. No differences were found for Hunter L* color, Minolta reflectance, and ultimate pH. 展开更多
关键词 SWINE meat quality intramuscular fat ultrasound technology
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