PiggyBac transposon has demonstrated its long-term and stable transposition on genomes of various species but lacking of the evidence on farm animal genomes. In this study, we constructed a piggyBac transposon marked ...PiggyBac transposon has demonstrated its long-term and stable transposition on genomes of various species but lacking of the evidence on farm animal genomes. In this study, we constructed a piggyBac transposon marked with enhanced green fluorescent protein （eGFP） and showed efficient transposition in porcine somatic cells and cloned embryos. Our results demonstrated that piggyBac transposase could efficiently catalyze transposition in porcine fetal fibroblast cells, as well as in embryos. PiggyBac transposition generated 18-fold more eGFP-positive cell colonies compared to pEGFP-C1 random insertion mutagenesis, but excessive transposase might affect the transfection rate. Also piggyBac mediated 4-fold more eGFP expression than random insertion in cells and 17-fold in cloned embryos at mRNA level. When the mutagenized cells were used for somatic cell nuclear transfer （SCNT）, the cleavage rate and blastocyst rate of constructed embryos harboring piggyBac transposition had no difference with random insertion group. This study provides key information on the piggyBac transposon system as a tool for creating transgenic pigs.展开更多
Raising Min pigs(MPs)in northern China is challenging,because of harsh environmental conditions in winter.There is a hypothesis that MPs can tolerate cold stress better than Large White pigs(LWPs)and their oocyte comp...Raising Min pigs(MPs)in northern China is challenging,because of harsh environmental conditions in winter.There is a hypothesis that MPs can tolerate cold stress better than Large White pigs(LWPs)and their oocyte competence is particularly protected.Over a period of 3 years,multiple cohorts of MPs and LWPs under different environmental conditions were studied in this paper.Follistatin(FST)serum level was used as a marker for swine oocyte competence.Oocytes were collected from the swine and their maturation rate,cleavage rate and blastocyst rate were measured.Lastly,mRNA expression in ovary and longissimus dorsi for the genes FST,luteinizing hormone receptor(LHR)and cold-inducible RNA-binding protein(CIRP)was measured.Under cold stress,serum follistatin concentrations of both LWPs and MPs were markedly increased,and they were more in LWPs than in MPs.However,oocyte competence was found to be unaffected by cold stress in MPs.It was found that in a separate group of LWPs,oocyte competence was lower in samples collected in winter than that in other seasons.Interestingly,follistatin levels in the follicular fluid of MPs showed no change with cold stress,and serum follistatin levels were increased in sows.Gene expression analysis showed a significant reduction in FST,LHR and CIRP expressions under cold stress in longissimus dorsi,but not in the ovaries.It was found that MPs were better able to maintain oocyte competence under cold stress,which might be due to improved regulation of follistatin in follicular fluid,and MPs appeared likely an adaptation to the harsh conditions that they faced under domestication in northern China.展开更多
基于视频的生猪行为跟踪和识别对于实现精细化养殖具有重要价值。为了应对群养生猪多目标跟踪任务中由猪只外观相似、遮挡交互等因素带来的挑战,研究提出了基于PigsTrack跟踪器的群养生猪多目标跟踪方法。PigsTrack跟踪器利用高性能YOLO...基于视频的生猪行为跟踪和识别对于实现精细化养殖具有重要价值。为了应对群养生猪多目标跟踪任务中由猪只外观相似、遮挡交互等因素带来的挑战,研究提出了基于PigsTrack跟踪器的群养生猪多目标跟踪方法。PigsTrack跟踪器利用高性能YOLOX网络降低目标误检与漏检率,采用Transformer模型获取具有良好区分特性的目标外观特征;基于OC-SORT(observation-centric sort)的思想,通过集成特征匹配、IoU匹配和遮挡恢复匹配策略实现群养生猪的准确跟踪。基于PBVD(pigs behaviours video dataset)数据集的试验结果表明,PigsTrack跟踪器的HOTA(higher order tracking accuracy),MOTA(multiple object tracking accuracy)和IDF1得分(identification F1 score)分别为85.66%、98.59%和99.57%,相较于现有算法的最高精度,分别提高了3.71、0.03和2.05个百分点,证明了PigsTrack跟踪器在解决外观相似和遮挡交互引起的跟踪过程中身份跳变问题方面的有效性。随后,利用Slowfast网络对PigsTrack跟踪器的跟踪结果进行了典型行为统计,结果显示PigsTrack在群养生猪个体行为统计方面更准确。此外,通过在ABVD(aggressive-behavior video)数据集上的试验,PigsTrack跟踪器的HOTA、MOTA和IDF1得分分别为69.14%、94.82%和90.11%,相对于现有算法的最高精度,提高了5.33、0.57和8.60个百分点,验证了PigsTrack跟踪器在群养生猪跟踪任务中的有效性。总而言之,PigsTrack跟踪器能够有效应对外观相似和遮挡交互等挑战,实现了准确的生猪多目标跟踪,并在行为统计方面展现出更高的准确性,为生猪养殖领域的研究和实际应用提供了有价值的指导。展开更多
A total of 72 growing-finishing pigs（56 days old） were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs...A total of 72 growing-finishing pigs（56 days old） were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs were allotted to three groups（four pens/group and six pigs/pen）. Diet treatments were a corn-soybean meal（control group） and a corn-soybean meal with 1% probiotics（probiotics group） or 80 mg kg-1colistin sulfate（antibiotics group）. Pigs were slaughtered when pigs of the lightest averaged 90 kg（157 days of age）. There was no difference（p＆gt;0.05） in average daily gain or feed/gain, and the average daily feed intake of the antibiotics group was higher than that of the probiotics and the control groups（p＆lt;0.05）. Diet supplemented with probiotics reduced the drip loss and cooking loss of pork（p＆lt;0.05）. Dietary supplementation with probiotics significantly decreased the content of triglycerides, lowdensity lipoprotein and urea nitrogen in serum（p＆lt;0.05）, and increased the content of high-density lipoprotein in serum（p＆lt;0.05）. These results suggested improving effects of dietary probiotics on pork quality and serum metabolism in growing-finishing pigs.展开更多
The follicle stimulating hormone beta-subunit （FSHβ） gene plays an important role in piglets. Marker-assisted selection （MAS） in conjunction with traditional selection methods is most effective for improving the ...The follicle stimulating hormone beta-subunit （FSHβ） gene plays an important role in piglets. Marker-assisted selection （MAS） in conjunction with traditional selection methods is most effective for improving the piglets breeding traits. To find the new SNPs, the polymorphism of it in six pig breeds （Min, Landrace, Yorkshine, Duroc, wild boar, and wild boar × Landrace） was analyzed by using PCR-SSCP and was further compared with each other. Polymorphism was found and the sequencing results showed that there was one silent mutation on exon2 （C48T） and two mutations on exon3 （T422C and A514G）. Genotype distribution of FSHβ in Min and wild boar on exon3 was in accordance with Hardy-Weinberg Law.展开更多
The method of PCR RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I...The method of PCR RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I pigs.The results show that the ESR and FSH β genes are the major genes of litter size;the heterozygous genotype for ESR as well as for FSH β locus has a more litter size than others.However,the combined genotype ABDD means a more litter size than other genotypes,the frequencies of the heterozygous genotypes in Sanjiang and Junmu I are lower than in Min which maybe one of the most important reasons for the little litter sizes of those two breeds.展开更多
基金Supported by the National Projects of Genetic Modified Organism Breeding Technology (2008ZX08006-002)the State Transgenic Research Programme of China (2008ZX08006-002)
文摘PiggyBac transposon has demonstrated its long-term and stable transposition on genomes of various species but lacking of the evidence on farm animal genomes. In this study, we constructed a piggyBac transposon marked with enhanced green fluorescent protein （eGFP） and showed efficient transposition in porcine somatic cells and cloned embryos. Our results demonstrated that piggyBac transposase could efficiently catalyze transposition in porcine fetal fibroblast cells, as well as in embryos. PiggyBac transposition generated 18-fold more eGFP-positive cell colonies compared to pEGFP-C1 random insertion mutagenesis, but excessive transposase might affect the transfection rate. Also piggyBac mediated 4-fold more eGFP expression than random insertion in cells and 17-fold in cloned embryos at mRNA level. When the mutagenized cells were used for somatic cell nuclear transfer （SCNT）, the cleavage rate and blastocyst rate of constructed embryos harboring piggyBac transposition had no difference with random insertion group. This study provides key information on the piggyBac transposon system as a tool for creating transgenic pigs.
基金Supported by the National Natural Science Foundation of China(32172696,U20A2052,31872980)Heilongjiang Provincial Scientific Research Business Fund Project(CZKYF2020A004,CZKYF2021-2-C025)Heilongjiang Provincial Natural Science Foundation(LH2019C064)。
文摘Raising Min pigs(MPs)in northern China is challenging,because of harsh environmental conditions in winter.There is a hypothesis that MPs can tolerate cold stress better than Large White pigs(LWPs)and their oocyte competence is particularly protected.Over a period of 3 years,multiple cohorts of MPs and LWPs under different environmental conditions were studied in this paper.Follistatin(FST)serum level was used as a marker for swine oocyte competence.Oocytes were collected from the swine and their maturation rate,cleavage rate and blastocyst rate were measured.Lastly,mRNA expression in ovary and longissimus dorsi for the genes FST,luteinizing hormone receptor(LHR)and cold-inducible RNA-binding protein(CIRP)was measured.Under cold stress,serum follistatin concentrations of both LWPs and MPs were markedly increased,and they were more in LWPs than in MPs.However,oocyte competence was found to be unaffected by cold stress in MPs.It was found that in a separate group of LWPs,oocyte competence was lower in samples collected in winter than that in other seasons.Interestingly,follistatin levels in the follicular fluid of MPs showed no change with cold stress,and serum follistatin levels were increased in sows.Gene expression analysis showed a significant reduction in FST,LHR and CIRP expressions under cold stress in longissimus dorsi,but not in the ovaries.It was found that MPs were better able to maintain oocyte competence under cold stress,which might be due to improved regulation of follistatin in follicular fluid,and MPs appeared likely an adaptation to the harsh conditions that they faced under domestication in northern China.
文摘基于视频的生猪行为跟踪和识别对于实现精细化养殖具有重要价值。为了应对群养生猪多目标跟踪任务中由猪只外观相似、遮挡交互等因素带来的挑战,研究提出了基于PigsTrack跟踪器的群养生猪多目标跟踪方法。PigsTrack跟踪器利用高性能YOLOX网络降低目标误检与漏检率,采用Transformer模型获取具有良好区分特性的目标外观特征;基于OC-SORT(observation-centric sort)的思想,通过集成特征匹配、IoU匹配和遮挡恢复匹配策略实现群养生猪的准确跟踪。基于PBVD(pigs behaviours video dataset)数据集的试验结果表明,PigsTrack跟踪器的HOTA(higher order tracking accuracy),MOTA(multiple object tracking accuracy)和IDF1得分(identification F1 score)分别为85.66%、98.59%和99.57%,相较于现有算法的最高精度,分别提高了3.71、0.03和2.05个百分点,证明了PigsTrack跟踪器在解决外观相似和遮挡交互引起的跟踪过程中身份跳变问题方面的有效性。随后,利用Slowfast网络对PigsTrack跟踪器的跟踪结果进行了典型行为统计,结果显示PigsTrack在群养生猪个体行为统计方面更准确。此外,通过在ABVD(aggressive-behavior video)数据集上的试验,PigsTrack跟踪器的HOTA、MOTA和IDF1得分分别为69.14%、94.82%和90.11%,相对于现有算法的最高精度,提高了5.33、0.57和8.60个百分点,验证了PigsTrack跟踪器在群养生猪跟踪任务中的有效性。总而言之,PigsTrack跟踪器能够有效应对外观相似和遮挡交互等挑战,实现了准确的生猪多目标跟踪,并在行为统计方面展现出更高的准确性,为生猪养殖领域的研究和实际应用提供了有价值的指导。
基金Supported by China Agriculture Research System(CARS-36)Program for Innovative Research Team of Universities in Heilongjiang Province
文摘A total of 72 growing-finishing pigs（56 days old） were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs were allotted to three groups（four pens/group and six pigs/pen）. Diet treatments were a corn-soybean meal（control group） and a corn-soybean meal with 1% probiotics（probiotics group） or 80 mg kg-1colistin sulfate（antibiotics group）. Pigs were slaughtered when pigs of the lightest averaged 90 kg（157 days of age）. There was no difference（p＆gt;0.05） in average daily gain or feed/gain, and the average daily feed intake of the antibiotics group was higher than that of the probiotics and the control groups（p＆lt;0.05）. Diet supplemented with probiotics reduced the drip loss and cooking loss of pork（p＆lt;0.05）. Dietary supplementation with probiotics significantly decreased the content of triglycerides, lowdensity lipoprotein and urea nitrogen in serum（p＆lt;0.05）, and increased the content of high-density lipoprotein in serum（p＆lt;0.05）. These results suggested improving effects of dietary probiotics on pork quality and serum metabolism in growing-finishing pigs.
基金Supported by National Key Technology R&D Program in the 11th Five Year Plan of China (2008BADB2B01)
文摘The follicle stimulating hormone beta-subunit （FSHβ） gene plays an important role in piglets. Marker-assisted selection （MAS） in conjunction with traditional selection methods is most effective for improving the piglets breeding traits. To find the new SNPs, the polymorphism of it in six pig breeds （Min, Landrace, Yorkshine, Duroc, wild boar, and wild boar × Landrace） was analyzed by using PCR-SSCP and was further compared with each other. Polymorphism was found and the sequencing results showed that there was one silent mutation on exon2 （C48T） and two mutations on exon3 （T422C and A514G）. Genotype distribution of FSHβ in Min and wild boar on exon3 was in accordance with Hardy-Weinberg Law.
文摘The method of PCR RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I pigs.The results show that the ESR and FSH β genes are the major genes of litter size;the heterozygous genotype for ESR as well as for FSH β locus has a more litter size than others.However,the combined genotype ABDD means a more litter size than other genotypes,the frequencies of the heterozygous genotypes in Sanjiang and Junmu I are lower than in Min which maybe one of the most important reasons for the little litter sizes of those two breeds.
