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Research on multi-view collaborative detection system for UAV swarms based on Pix2Pix framework and BAM attention mechanism
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作者 Yan Ding Qingxin Cao +2 位作者 Bozhi Zhang Peilin Li Zhongjiao Shi 《Defence Technology(防务技术)》 2025年第4期213-226,共14页
Drone swarm systems,equipped with photoelectric imaging and intelligent target perception,are essential for reconnaissance and strike missions in complex and high-risk environments.They excel in information sharing,an... Drone swarm systems,equipped with photoelectric imaging and intelligent target perception,are essential for reconnaissance and strike missions in complex and high-risk environments.They excel in information sharing,anti-jamming capabilities,and combat performance,making them critical for future warfare.However,varied perspectives in collaborative combat scenarios pose challenges to object detection,hindering traditional detection algorithms and reducing accuracy.Limited angle-prior data and sparse samples further complicate detection.This paper presents the Multi-View Collaborative Detection System,which tackles the challenges of multi-view object detection in collaborative combat scenarios.The system is designed to enhance multi-view image generation and detection algorithms,thereby improving the accuracy and efficiency of object detection across varying perspectives.First,an observation model for three-dimensional targets through line-of-sight angle transformation is constructed,and a multi-view image generation algorithm based on the Pix2Pix network is designed.For object detection,YOLOX is utilized,and a deep feature extraction network,BA-RepCSPDarknet,is developed to address challenges related to small target scale and feature extraction challenges.Additionally,a feature fusion network NS-PAFPN is developed to mitigate the issue of deep feature map information loss in UAV images.A visual attention module(BAM)is employed to manage appearance differences under varying angles,while a feature mapping module(DFM)prevents fine-grained feature loss.These advancements lead to the development of BA-YOLOX,a multi-view object detection network model suitable for drone platforms,enhancing accuracy and effectively targeting small objects. 展开更多
关键词 Drone swarm systems Reconnaissance and strike Image generation Multi-view detection Pix2Pix framework Attention mechanism
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Multi-objective optimization framework in the modeling of belief rule-based systems with interpretability-accuracy trade-off
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作者 YOU Yaqian SUN Jianbin +1 位作者 TAN Yuejin JIANG Jiang 《Journal of Systems Engineering and Electronics》 2025年第2期423-435,共13页
The belief rule-based(BRB)system has been popular in complexity system modeling due to its good interpretability.However,the current mainstream optimization methods of the BRB systems only focus on modeling accuracy b... The belief rule-based(BRB)system has been popular in complexity system modeling due to its good interpretability.However,the current mainstream optimization methods of the BRB systems only focus on modeling accuracy but ignore the interpretability.The single-objective optimization strategy has been applied in the interpretability-accuracy trade-off by inte-grating accuracy and interpretability into an optimization objec-tive.But the integration has a greater impact on optimization results with strong subjectivity.Thus,a multi-objective optimiza-tion framework in the modeling of BRB systems with inter-pretability-accuracy trade-off is proposed in this paper.Firstly,complexity and accuracy are taken as two independent opti-mization goals,and uniformity as a constraint to give the mathe-matical description.Secondly,a classical multi-objective opti-mization algorithm,nondominated sorting genetic algorithm II(NSGA-II),is utilized as an optimization tool to give a set of BRB systems with different accuracy and complexity.Finally,a pipeline leakage detection case is studied to verify the feasibility and effectiveness of the developed multi-objective optimization.The comparison illustrates that the proposed multi-objective optimization framework can effectively avoid the subjectivity of single-objective optimization,and has capability of joint optimiz-ing the structure and parameters of BRB systems with inter-pretability-accuracy trade-off. 展开更多
关键词 belief rule-based(BRB)systems INTERPRETABILITY multi-objective optimization nondominated sorting genetic algo-rithm II(NSGA-II) pipeline leakage detection.
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Launch dynamics modeling and simulation of box-type multiple launch rocket system considering plane clearance contact
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作者 Jinxin Tang Guoping Wang +3 位作者 Genyang Wu Yutian Sun Lilin Gu Xiaoting Rui 《Defence Technology(防务技术)》 2025年第5期105-123,共19页
As the performance of the box-type multiple launch rocket system(BMLRS)improves,its mechanical structures,particularly the plane clearance design between the slider on the rocket and the guide inside the launch canist... As the performance of the box-type multiple launch rocket system(BMLRS)improves,its mechanical structures,particularly the plane clearance design between the slider on the rocket and the guide inside the launch canister,have grown increasingly complex.However,deficiencies still exist in the current launch modeling theory for BMLRS.In this study,a multi-rigid-flexible-body launch dynamics model coupling the launch platform and rocket was established using the multibody system transfer matrix method and the Newton-Euler formulation.Furthermore,considering the bending of the launch canister,a detection algorithm for slider-guide plane clearance contact was proposed.To quantify the contact force and friction effect between the slider and guide,the contact force model and modified Coulomb model were introduced.Both the modal and launch tests were conducted.Additionally,the modal convergence was verified.By comparing the modal experiments and simulation results,the maximum relative error of the eigenfrequency is 3.29%.thereby verifying the accuracy of the developed BMLRS dynamics model.Furthermore,the launch test validated the proposed plane clearance contact model.Moreover,the study investigated the influence of various model parameters on the dynamic characteristics of BMLRS,including launch canister bending stiffness,slider and guide material,slider-guide clearance,slider length and layout.This analysis of influencing factors provides a foundation for future optimization in BMLRS design. 展开更多
关键词 Box-type multiple launch rocket system Launch dynamics Plane clearance contact Contact detection algorithm Multibody system transfer matrix method(MSTMM)
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Industrial shape detecting system of cold rolling strip 被引量:9
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作者 杨利坡 于丙强 +1 位作者 丁栋 刘宏民 《Journal of Central South University》 SCIE EI CAS 2012年第4期994-1001,共8页
A high-precision shape detecting system of cold rolling strip is developed to meet industrial application, which mainly consists of the shape detecting roller, the collecting ring, the digital signal processing (DSP... A high-precision shape detecting system of cold rolling strip is developed to meet industrial application, which mainly consists of the shape detecting roller, the collecting ring, the digital signal processing (DSP) shape signal processing board and the shape control model. Based on the shape detecting principle, the shape detecting roller is designed with a new integral structure for improving the precision of shape detecting and avoiding scratching strip surface. Based on the DSP technology, the DSP shape signal processing circuit board is designed and embedded in the shape detecting system for the reliability and stability of shape signal processing. The shape detecting system was successfully used in Angang 1 250 mm HC 6-high reversible cold rolling mill. The precision of shape detecting is 0.2 I and the shape deviation is controlled within 6 1 after the close loop shape control is input. 展开更多
关键词 shape detecting digital signal processing (DSP) shape signal processing close loop shape control cold rolling strip
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Spectral Target-Detecting System Using Sine-Wave Modulation 被引量:1
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作者 DENG Wei ZHAO Chun-jiang +2 位作者 ZHANG Lu-da CHENG Li-ping Andrew Landers 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2011年第10期2771-2777,共7页
Target detection is one of the key technology of precision chemical application.Previously the digital coding modulation technique was commonly used to emit and receive the optical signal in the target detection syste... Target detection is one of the key technology of precision chemical application.Previously the digital coding modulation technique was commonly used to emit and receive the optical signal in the target detection systems previously in China.It was difficult to adjust the output power,and the anti-interference ability was weak in these systems.In order to resolve these problems,the target detection method based on analog sine-wave modulation was studied.The spectral detecting system was set up in the aspects of working principle,electric circuit,and optical path.Lab testing was performed.The results showed that the reflected signal from the target varied inversely with detection distances.It indicated that it was feasible to establish the target detection system using analog sine-wave modulation technology.Furthermore,quantitative measurement of the reflected optical signal for near-infrared and visible light could be achieved by using this system.The research laid the foundation for the future development of the corresponding instrument. 展开更多
关键词 Sine-wave modulation ANALOG Target detection Optical spectrum
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Fault diagnosis method of link control system for gravitational wave detection 被引量:1
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作者 GAO Ai XU Shengnan +2 位作者 ZHAO Zichen SHANG Haibin XU Rui 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第4期922-931,共10页
To maintain the stability of the inter-satellite link for gravitational wave detection,an intelligent learning monitoring and fast warning method of the inter-satellite link control system failure is proposed.Differen... To maintain the stability of the inter-satellite link for gravitational wave detection,an intelligent learning monitoring and fast warning method of the inter-satellite link control system failure is proposed.Different from the traditional fault diagnosis optimization algorithms,the fault intelligent learning method pro-posed in this paper is able to quickly identify the faults of inter-satellite link control system despite the existence of strong cou-pling nonlinearity.By constructing a two-layer learning network,the method enables efficient joint diagnosis of fault areas and fault parameters.The simulation results show that the average identification time of the system fault area and fault parameters is 0.27 s,and the fault diagnosis efficiency is improved by 99.8%compared with the traditional algorithm. 展开更多
关键词 large scale multi-satellite formation gravitational wave detection laser link monitoring fault diagnosis deep learning
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The concept of sUAS/DL-based system for detecting and classifying abandoned small firearms
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作者 Jungmok Ma Oleg A.Yakimenko 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2023年第12期23-31,共9页
Military object detection and identification is a key capability in surveillance and reconnaissance.It is a major factor in warfare effectiveness and warfighter survivability.Inexpensive,portable,and rapidly deployabl... Military object detection and identification is a key capability in surveillance and reconnaissance.It is a major factor in warfare effectiveness and warfighter survivability.Inexpensive,portable,and rapidly deployable small unmanned aerial systems(s UAS)in conjunction with powerful deep learning(DL)based object detection models are expected to play an important role for this application.To prove overall feasibility of this approach,this paper discusses some aspects of designing and testing of an automated detection system to locate and identify small firearms left at the training range or at the battlefield.Such a system is envisioned to involve an s UAS equipped with a modern electro-optical(EO)sensor and relying on a trained convolutional neural network(CNN).Previous study by the authors devoted to finding projectiles on the ground revealed certain challenges such as small object size,changes in aspect ratio and image scale,motion blur,occlusion,and camouflage.This study attempts to deal with these challenges in a realistic operational scenario and go further by not only detecting different types of firearms but also classifying them into different categories.This study used a YOLOv2CNN(Res Net-50 backbone network)to train the model with ground truth data and demonstrated a high mean average precision(m AP)of 0.97 to detect and identify not only small pistols but also partially occluded rifles. 展开更多
关键词 Small firearms Object detection Deep learning Small unmanned aerial systems
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Low-complexity signal detection for massive MIMO systems via trace iterative method
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作者 IMRAN A.Khoso ZHANG Xiaofei +2 位作者 ABDUL Hayee Shaikh IHSAN A.Khoso ZAHEER Ahmed Dayo 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期549-557,共9页
Linear minimum mean square error(MMSE)detection has been shown to achieve near-optimal performance for massive multiple-input multiple-output(MIMO)systems but inevitably involves complicated matrix inversion,which ent... Linear minimum mean square error(MMSE)detection has been shown to achieve near-optimal performance for massive multiple-input multiple-output(MIMO)systems but inevitably involves complicated matrix inversion,which entails high complexity.To avoid the exact matrix inversion,a considerable number of implicit and explicit approximate matrix inversion based detection methods is proposed.By combining the advantages of both the explicit and the implicit matrix inversion,this paper introduces a new low-complexity signal detection algorithm.Firstly,the relationship between implicit and explicit techniques is analyzed.Then,an enhanced Newton iteration method is introduced to realize an approximate MMSE detection for massive MIMO uplink systems.The proposed improved Newton iteration significantly reduces the complexity of conventional Newton iteration.However,its complexity is still high for higher iterations.Thus,it is applied only for first two iterations.For subsequent iterations,we propose a novel trace iterative method(TIM)based low-complexity algorithm,which has significantly lower complexity than higher Newton iterations.Convergence guarantees of the proposed detector are also provided.Numerical simulations verify that the proposed detector exhibits significant performance enhancement over recently reported iterative detectors and achieves close-to-MMSE performance while retaining the low-complexity advantage for systems with hundreds of antennas. 展开更多
关键词 signal detection LOW-COMPLEXITY linear minimum mean square error(MMSE) massive multiple-input multiple-output(MIMO) trace iterative method(TIM)
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Detection of geohazards caused by human disturbance activities based on convolutional neural networks
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作者 ZHANG Heng ZHANG Diandian +1 位作者 YUAN Da LIU Tao 《水利水电技术(中英文)》 北大核心 2025年第S1期731-738,共8页
Human disturbance activities is one of the main reasons for inducing geohazards.Ecological impact assessment metrics of roads are inconsistent criteria and multiple.From the perspective of visual observation,the envir... Human disturbance activities is one of the main reasons for inducing geohazards.Ecological impact assessment metrics of roads are inconsistent criteria and multiple.From the perspective of visual observation,the environment damage can be shown through detecting the uncovered area of vegetation in the images along road.To realize this,an end-to-end environment damage detection model based on convolutional neural network is proposed.A 50-layer residual network is used to extract feature map.The initial parameters are optimized by transfer learning.An example is shown by this method.The dataset including cliff and landslide damage are collected by us along road in Shennongjia national forest park.Results show 0.4703 average precision(AP)rating for cliff damage and 0.4809 average precision(AP)rating for landslide damage.Compared with YOLOv3,our model shows a better accuracy in cliff and landslide detection although a certain amount of speed is sacrificed. 展开更多
关键词 convolutional neural network DETECTION environment damage CLIFF LANDSLIDE
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MSFNet:A Network for Lunar Impact Crater Detection Based on Enhanced Feature Fusion with Digital Elevation Model
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作者 HE Weidong LAI Jialong +3 位作者 ZHONG Zhicheng CUI Feifei XU Yi ZHANG Xiaoping 《深空探测学报(中英文)》 北大核心 2025年第2期190-204,共15页
Lunar impact crater detection is crucial for lunar surface studies and spacecraft landing missions,yet deep learning still struggles with accurately detecting small craters,especially when relying on incomplete catalo... Lunar impact crater detection is crucial for lunar surface studies and spacecraft landing missions,yet deep learning still struggles with accurately detecting small craters,especially when relying on incomplete catalogs.In this work,we integrate Digital Elevation Model(DEM)data to construct a high-quality dataset enriched with slope information,enabling a detailed analysis of crater features and effectively improving detection performance in complex terrains and low-contrast areas.Based on this foundation,we propose a novel two-stage detection network,MSFNet,which leverages multi-scale adaptive feature fusion and multisize ROI pooling to enhance the recognition of craters across various scales.Experimental results demonstrate that MSFNet achieves an F1 score of 74.8%on Test Region1 and a recall rate of 87%for craters with diameters larger than 2 km.Moreover,it shows exceptional performance in detecting sub-kilometer craters by successfully identifying a large number of high-confidence,previously unlabeled targets with a low false detection rate confirmed through manual review.This approach offers an efficient and reliable deep learning solution for lunar impact crater detection. 展开更多
关键词 object detection deep learning impact crater DEM
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Recent Advances in Non-Enzymatic Electrochemical Sensors for Theophylline Detection
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作者 Ernis Gustria Putri Yulia M T A +5 位作者 Syauqi Muhammad Iqbal Jiwanti Prastika Krisma Hartati Yeni Wahyuni Kondo Takeshi Anjani Qonita Kurnia Gunlazuardi Jarnuzi 《电化学(中英文)》 北大核心 2025年第3期1-24,共24页
Detection of target analytes at low concentrations is significant in various fields,including pharmaceuticals,healthcare,and environmental protection.Theophylline(TP),a natural alkaloid used as a bronchodilator to tre... Detection of target analytes at low concentrations is significant in various fields,including pharmaceuticals,healthcare,and environmental protection.Theophylline(TP),a natural alkaloid used as a bronchodilator to treat respiratory disorders such as asthma,bronchitis,and emphysema,has a narrow therapeutic window with a safe plasma concentration ranging from 55.5-111.0μmol·L^(-1)in adults.Accurate monitoring of TP levels is essential because too low or too high can cause se-rious side effects.In this regard,non-enzymatic electrochemical sensors offer a practical solution with rapidity,portability,and high sensitivity.This article aims to provide a comprehensive review of the recent developments of non-enzymatic electrochemical sensors for TP detection,highlighting the basic principles,electro-oxidation mechanisms,catalytic effects,and the role of modifying materials on electrode performance.Carbon-based electrodes such as glassy carbon electrodes(GCEs),carbon paste electrodes(CPEs),and carbon screen-printed electrodes(SPCEs)have become the primary choices for non-enzymatic sensors due to their chemical stability,low cost,and flexibility in modification.This article identifies the sig-nificant contribution of various modifying materials,including nanomaterials such as carbon nanotubes(CNTs),graphene,metal oxides,and multi-element nanocomposites.These modifications enhance sensors’electron transfer,sensitivity,and selectivity in detecting TP at low concentrations in complex media such as blood plasma and pharmaceutical samples.The electro-oxidation mechanism of TP is also discussed in depth,emphasizing the hydroxyl and carbonyl reaction pathways strongly influenced by pH and electrode materials.These mechanisms guide the selection of the appropriate electrode ma-terial for a particular application.The main contribution of this article is to identify superior modifying materials that can improve the performance of non-enzymatic electrochemical sensors.In a recent study,the combination of multi-element nanocomposites based on titanium dioxide(TiO_(2)),CNTs,and gold nanoparticles(AuNPs)resulted in the lowest detection limit of 3×10^(-5)μmol·L^(-1),reflecting the great potential of these materials for developing high-performance electrochemical sensors.The main conclusion of this article is the importance of a multidisciplinary approach in electrode material design to support the sensitivity and selectivity of TP detection.In addition,there is still a research gap in understanding TP’s more detailed oxidation mechanism,especially under pH variations and complex environments.Therefore,further research on electrode modification and analysis of the TP oxidation mechanism are urgently needed to improve the accuracy and sta-bility of the sensor while expanding its applications in pharmaceutical monitoring and medical diagnostics.By integrating various innovative materials and technical approaches,this review is expected to be an essential reference for developing efficient and affordable non-enzymatic electrochemical sensors. 展开更多
关键词 Theophylline detection Non-enzymatic sensors Electrochemical sensors Modifier electrode Reaction mechanism
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An azo-based fluorescent probe for the detection of hypoxic tumor cells
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作者 ZHANG Lei HE Cheng JIAO Yang 《无机化学学报》 北大核心 2025年第6期1162-1172,共11页
The abnormal metabolic activity of the tumor can increase the oxygen consumption in tumor cells,and the poor blood perfusion often happens in tumor regions as well,which are the main reasons that result in a hypoxic s... The abnormal metabolic activity of the tumor can increase the oxygen consumption in tumor cells,and the poor blood perfusion often happens in tumor regions as well,which are the main reasons that result in a hypoxic situation in the tumor.A fluorescence probe,AQD,with selective response toward hypoxia was designed for the detection of hypoxic tumor cells,which was obtained by the covalent connection of a large planar conjugated fluorophore with good fluorescence stability and a N,N-dimethylaniline moiety via the azo bond.The introduction of the azo bond in AQD caused significant fluorescence emission quenching,and the probe was reduced under hypoxic conditions to release the fluorophore via breaking the azo bond,resulting in the gradual recovery of fluorescence emission.Probe AQD exhibited a remarkable fluorescence response in hypoxic conditions,high selectivity,and good biocompatibility,which was successfully used for the imaging of hypoxic tumor cells and realized the detection of hypoxic A549 cells. 展开更多
关键词 hypoxia response azo compound fluorescent probe fluorescence detection cell imaging
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Design,synthesis,and detection of Al^(3+)of two zinc complexes based on Schiff base ligands
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作者 YANG Yuanyu XUE Jianhua +3 位作者 BAI Yujia CUI Lulu YANG Dongdong MA Qi 《无机化学学报》 北大核心 2025年第6期1207-1216,共10页
Two new complexes,[Zn_(2)(L1)(HL1)(NO_(3))]·CH_(3)OH(1)and[Zn_(3)(L2)(L3)_(3)Cl]·CH_(3)OH(2),were successfully synthesized by‘one-pot’method based on cinnoline-3-ylhydrazine ligand and zinc with 2-hydroxy-... Two new complexes,[Zn_(2)(L1)(HL1)(NO_(3))]·CH_(3)OH(1)and[Zn_(3)(L2)(L3)_(3)Cl]·CH_(3)OH(2),were successfully synthesized by‘one-pot’method based on cinnoline-3-ylhydrazine ligand and zinc with 2-hydroxy-4-methoxybenzaldehyde and 2-hydroxy-3-methoxybenzaldehyde ligands,respectively,where H_(2)L1=5-methoxy-2-(phthalazin-1-ylhydrazonomethyl)-phenol,H_(2)L2=2-methoxy-6-(phthalazin-1-yl-hydrazonomethyl)-phenol,HL3=2-(1,8-dihydro-[1,2,4]triazolo[3,4-α]phthalazin-3-yl)-6-methoxy-phenol.Complexes 1 and 2 were characterized by infrared spectroscopy,elemental analysis,single-crystal X-ray diffraction,powder X-ray diffraction,etc.It is worth noting that the cinnolin-3-yl-hydrazine ligand and 2-hydroxy-3-methoxybenzaldehyde form two types of Schiff bases(H_(2)L2 and HL3)when in situ reacting and coordinating with Zn(Ⅱ),and HL3 also has two coordination modes.In addition,the fluorescence performance showed that complex 1 can achieve selective and sensitive sensing of Al^(3+)in water with a detection limit of 6.37μmol·L^(-1).CCDC:2413978,1;2413979,2. 展开更多
关键词 Zn-complex Schiff base FLUORESCENCE Al^(3+)detection
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Research on SAR Image Lightweight Detection Based on Improved YOLOV8
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作者 WANG Qing SI Zhan-jun 《印刷与数字媒体技术研究》 北大核心 2025年第1期93-100,共8页
In recent years,with the development of synthetic aperture radar(SAR)technology and the widespread application of deep learning,lightweight detection of SAR images has emerged as a research direction.The ultimate goal... In recent years,with the development of synthetic aperture radar(SAR)technology and the widespread application of deep learning,lightweight detection of SAR images has emerged as a research direction.The ultimate goal is to reduce computational and storage requirements while ensuring detection accuracy and reliability,making it an ideal choice for achieving rapid response and efficient processing.In this regard,a lightweight SAR ship target detection algorithm based on YOLOv8 was proposed in this study.Firstly,the C2f-Sc module was designed by fusing the C2f in the backbone network with the ScConv to reduce spatial redundancy and channel redundancy between features in convolutional neural networks.At the same time,the Ghost module was introduced into the neck network to effectively reduce model parameters and computational complexity.A relatively lightweight EMA attention mechanism was added to the neck network to promote the effective fusion of features at different levels.Experimental results showed that the Parameters and GFLOPs of the improved model are reduced by 8.5%and 7.0%when mAP@0.5 and mAP@0.5:0.95 are increased by 0.7%and 1.8%,respectively.It makes the model lightweight and improves the detection accuracy,which has certain application value. 展开更多
关键词 YOLOv8 Synthetic aperture radar image LIGHTWEIGHT Target detection
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Flatness detection method of splicing detector based on channel spectral dispersion
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作者 ZHAO Hong-chao ZHANG Xiao-qian AN Qi-chang 《中国光学(中英文)》 北大核心 2025年第4期889-898,共10页
For segmented detectors,surface flatness is critical as it directly influences both energy resolution and image clarity.Additionally,the limited adjustment range of the segmented detectors necessitates precise benchma... For segmented detectors,surface flatness is critical as it directly influences both energy resolution and image clarity.Additionally,the limited adjustment range of the segmented detectors necessitates precise benchmark construction.This paper proposes an architecture for detecting detector flatness based on channel spectral dispersion.By measuring the dispersion fringes for coplanar adjustment,the final adjustment residual is improved to better than 300 nm.This result validates the feasibility of the proposed technology and provides significant technical support for the development of next-generation large-aperture sky survey equipment. 展开更多
关键词 large aperture telescope segmented detector surface wavefront detection channel spectral dispersion
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Establishment of a field visualization detection method for multiplex recombinase polymerase amplification combined with CRISPR/Cas12a in genetically modified crops
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作者 YAN Jingying NI Liang +2 位作者 SHEN Xingyu LÜ Bingtao LI Yu 《浙江大学学报(农业与生命科学版)》 北大核心 2025年第3期391-401,共11页
With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a c... With the approval of more and more genetically modified(GM)crops in our country,GM safety management has become more important.Transgenic detection is a major approach for transgenic safety management.Nevertheless,a convenient and visual technique with low equipment requirements and high sensitivity for the field detection of GM plants is still lacking.On the basis of the existing recombinase polymerase amplification(RPA)technique,we developed a multiplex RPA(multi-RPA)method that can simultaneously detect three transgenic elements,including the cauliflower mosaic virus 35S gene(CaMV35S)promoter,neomycin phosphotransferaseⅡgene(NptⅡ)and hygromycin B phosphotransferase gene(Hyg),thus improving the detection rate.Moreover,we coupled this multi-RPA technique with the CRISPR/Cas12a reporter system,which enabled the detection results to be clearly observed by naked eyes under ultraviolet(UV)light(254 nm;which could be achieved by a portable UV flashlight),therefore establishing a multi-RPA visual detection technique.Compared with the traditional test strip detection method,this multi-RPA-CRISPR/Cas12a technique has the higher specificity,higher sensitivity,wider application range and lower cost.Compared with other polymerase chain reaction(PCR)techniques,it also has the advantages of low equipment requirements and visualization,making it a potentially feasible method for the field detection of GM plants. 展开更多
关键词 genetically modified crop recombinase polymerase amplification CRISPR/Cas12a field detection
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Pr/N co-doped biomass carbon dots with enhanced fluorescence for efficient detection of 2,4-dinitrophenylhydrazine
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作者 SUN Xuehua MA Min +4 位作者 LIU Jianting TIAN Rui CHAI Hongmei CUI Huali GAO Loujun 《无机化学学报》 北大核心 2025年第3期561-573,共13页
We used the natural product chamomile as a carbon source to synthesize praseodymium(Pr) and nitrogen(N) co-doped biomass carbon dots(Pr/N-BCDs) with remarkable luminescence properties by one-step hydrothermal method.C... We used the natural product chamomile as a carbon source to synthesize praseodymium(Pr) and nitrogen(N) co-doped biomass carbon dots(Pr/N-BCDs) with remarkable luminescence properties by one-step hydrothermal method.Compared with single N-doped BCDs(N-BCDs) and Pr-doped BCDs(Pr-BCDs),Pr/N-BCDs not only showed better fluorescence properties and stability but also achieved a significant increase in quantum yield of 12%.More importantly,under certain conditions,Pr/N-BCDs and 2,4-dinitrophenylhydrazide(2,4-DNPH) had significant fluorescence internal filtration effect(IFE) and dynamic quenching effect,and in the concentration range of0.50-20 μmol·L^(-1),the concentration of 2,4-DNPH had a good linear relationship with the fluorescence quenching signal,and the detection limit was as low as 2.1 nmol·L^(-1). 展开更多
关键词 co⁃doped carbon dots 2 4⁃DNPH fluorescence detection
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Ratiometric Fluorescence Detection Method for Calcium Ions and Manganese Ions Based on MXenes Quantum Dots
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作者 HUANG Wei ZHONG Shuang-yu +3 位作者 LI Chang LIN Chun-xiao RONG Ming-cong NIU Li 《分析测试学报》 北大核心 2025年第9期1889-1899,共11页
Calcium ions(Ca^(2+))and manganese ions(Mn^(2+))are essential for sustaining life activities and are key monitoring indicators in drinking water.Developing highly sensitive,selective,and portable detection methods for... Calcium ions(Ca^(2+))and manganese ions(Mn^(2+))are essential for sustaining life activities and are key monitoring indicators in drinking water.Developing highly sensitive,selective,and portable detection methods for Ca^(2+)and Mn^(2+)is significant for water quality monitoring and human health.In this paper,blue fluorescent Ti3C2 MXene-based quantum dots(MQDs,λ_(em)=445 nm)are prepared using Ti_(3)C_(2)MXene as the precursor.Through the chelation effect of ethylene diamine tetraacetic acid(EDTA),a blue and red dual-emission fluorescent probe,MQDs-EDTA-Eu^(3+)-DPA,was constructed.Herein,dipicolinic acid(DPA)acts as an absorbing ligand and significantly enhances the red fluorescence of europium ions(Eu^(3+))at 616 nm through the“antenna effect”.The blue fluorescence of MQDs serves as an internal reference signal.High concentrations of Ca^(2+)can quench the red fluorescence of Eu^(3+)-DPA;Mn^(2+)can be excited to emit purple fluorescence at 380 nm after coordinating with DPA,red fluorescence of Eu^(3+)-DPA serves as the internal reference signal.Based on the above two fluorescence intensity changes,ratiometric fluorescence detection methods for Ca^(2+)and Mn^(2+)are established.The fluorescence intensity ratio(IF_(616)/IF_(445))exhibits a linear relationship with Ca^(2+)in the range of 35-120μmol/L,with a detection limit of 5.98μmol/L.The fluorescence intensity ratio(IF_(380)/IF_(616))shows good linearity with Mn^(2+)in the range of 0-14μmol/L,with a detection limit of 28.6 nmol/L.This method was successfully applied to the quantitative analysis of Ca^(2+)and Mn^(2+)in commercially available mineral water(Nongfu Spring,Ganten,and Evergrande),with recovery rates of 80.6%-117%and relative standard deviations(RSD)of 0.76%-4.6%.Additionally,by preparing MQD-based fluorescent test strips,visual detections of Ca^(2+)and Mn^(2+)are achieved.This work demonstrates the application potential of MQDs in the field of visual fluorescence sensing of ions in water quality. 展开更多
关键词 calcium ion manganese ion Ti3C2 MXene-based quantum dots ratiometric fluorescence detection visual sensing
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Infrared small target detection algorithm via partial sum of the tensor nuclear norm and direction residual weighting
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作者 SUN Bin XIA Xing-Ling +1 位作者 FU Rong-Guo SHI Liang 《红外与毫米波学报》 北大核心 2025年第2期277-288,共12页
Aiming at the problem that infrared small target detection faces low contrast between the background and the target and insufficient noise suppression ability under the complex cloud background,an infrared small targe... Aiming at the problem that infrared small target detection faces low contrast between the background and the target and insufficient noise suppression ability under the complex cloud background,an infrared small target detection method based on the tensor nuclear norm and direction residual weighting was proposed.Based on converting the infrared image into an infrared patch tensor model,from the perspective of the low-rank nature of the background tensor,and taking advantage of the difference in contrast between the background and the target in different directions,we designed a double-neighborhood local contrast based on direction residual weighting method(DNLCDRW)combined with the partial sum of tensor nuclear norm(PSTNN)to achieve effective background suppression and recovery of infrared small targets.Experiments show that the algorithm is effective in suppressing the background and improving the detection ability of the target. 展开更多
关键词 infrared small target detection infrared patch tensor model partial sum of the tensor nuclear norm direction residual weighting
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Development of NIR Responsive Upconversion Nanosensor for Turn-on Detection of 4-Nonylphenol
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作者 HUANG Sili XU Kuncheng +5 位作者 YE Yiwen WEN Hongli CHEN Rihui SONG Wei CHEN Wei ABDUR Raheem Aleem 《发光学报》 北大核心 2025年第1期140-155,共16页
4-Nonylphenol(NP)is a kind of estrogen belonging to the endocrine disrupter,widely used in various agricultural and industrial goods.However,extensive use of NP with direct release to environment poses high risks to b... 4-Nonylphenol(NP)is a kind of estrogen belonging to the endocrine disrupter,widely used in various agricultural and industrial goods.However,extensive use of NP with direct release to environment poses high risks to both human health and ecosystems.Herein,for the first time,we developed near-infrared(NIR)responsive upconversion luminescence nanosensor for NP detection.The Förster resonance energy transfer based upconversion nanoparticles(UCNPs)-graphene oxide sensor offers highly selective and sensitive detection of NP in linear ranges of 5−200 ng/mL and 200−1000 ng/mL under 980 nm and 808 nm excitation,respectively,with LOD at 4.2 ng/mL.The sensors were successfully tested for NP detection in real liquid milk samples with excellent recovery results.The rare-earth fluoride based upconversion luminescence nanosensor with NIR excitation wavelength,holds promise for sensing food,environmental,and biological samples due to their high sensitivity,specific recognition,low LOD,negligible autofluorescence,along with the deep penetration of NIR excitation sources. 展开更多
关键词 Er^(3+)/Yb^(3+)/Nd^(3+) upconversion nanoparticles Förster resonance energy transfer ESTROGEN DETECTION
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