The dynamic changes in the malondialdehyde（MDA）, superoxide dismutase（SOD）, soluble sugar, proline, and soluble protein contents, as well as the relative electrolyte conductivity and the corresponding cold resista...The dynamic changes in the malondialdehyde（MDA）, superoxide dismutase（SOD）, soluble sugar, proline, and soluble protein contents, as well as the relative electrolyte conductivity and the corresponding cold resistance, of Cinnamomum bodinieri Level., C. camphora L., and C. caudiferum Kisterm were investigated during the winter months of October 2009 to April 2010. During the short period of temperature decline that lasted until mid-December, the changes in the relative electrolyte conductivity and MDA content with temperature were insignificant. In January, SOD activity continued to increase and then peaked as a result of rapid increases in soluble sugar, proline, soluble protein, as well as the inhibition of the relative electrolyte conductivity and decrease in MDA content. These physiological changes protected the camphor trees from cold damage during winter. From February to March, SOD activity and the soluble protein and proline contents increased with the increase in temperature. However, the relative electrolyte conductivity and MDA content decreased, indicating that the cell membrane damaged by low temperature was gradually being repaired. The cold dip in April led to slight increases in the relative electrolyte conductivity and MDA content. Using a fuzzy mathematics method, the cold resistance adaptability of the camphor trees was divided into three periods namely, the enhancement setting stage, the vigorous stage, and the reducing stage. The cold tolerance abilities were ranked as the following order： C. bodinieri Level〉C. camphora L.〉C. caudiferum Kisterm.展开更多
Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive ...Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water composition and content, and the changes in cell viability in the crowns, which contain the growing point, were studied during the period from cold acclimation to the deep freezing. The results showed that electrical conductivity which reflects the degree of plasma membrane damaged under low temperature was significantly correlated with the free water and the total water content. The malondialdehyde （MDA） content, which reflects the degree of membrane peroxidation, was very significantly correlated with superoxide dismutase （SOD）, peroxidase, and ascorbic acid. During the deep freezing period, the SOD activity and glutathione （GSH） content of the winter wheat varieties were relative to their cold resistance. During this period, the MDA stability, SOD, GSH, and the total water and the free water content might be used to identify the cold resistance of winter wheat varieties.展开更多
基金Supported by Youth Science Foundation from Henan University of Science and Technology(2013)
文摘The dynamic changes in the malondialdehyde（MDA）, superoxide dismutase（SOD）, soluble sugar, proline, and soluble protein contents, as well as the relative electrolyte conductivity and the corresponding cold resistance, of Cinnamomum bodinieri Level., C. camphora L., and C. caudiferum Kisterm were investigated during the winter months of October 2009 to April 2010. During the short period of temperature decline that lasted until mid-December, the changes in the relative electrolyte conductivity and MDA content with temperature were insignificant. In January, SOD activity continued to increase and then peaked as a result of rapid increases in soluble sugar, proline, soluble protein, as well as the inhibition of the relative electrolyte conductivity and decrease in MDA content. These physiological changes protected the camphor trees from cold damage during winter. From February to March, SOD activity and the soluble protein and proline contents increased with the increase in temperature. However, the relative electrolyte conductivity and MDA content decreased, indicating that the cell membrane damaged by low temperature was gradually being repaired. The cold dip in April led to slight increases in the relative electrolyte conductivity and MDA content. Using a fuzzy mathematics method, the cold resistance adaptability of the camphor trees was divided into three periods namely, the enhancement setting stage, the vigorous stage, and the reducing stage. The cold tolerance abilities were ranked as the following order： C. bodinieri Level〉C. camphora L.〉C. caudiferum Kisterm.
基金Supported by the Postdoctoral Science Foundation of Heilongjiang Province(2010RCB17)Doctoral Research Startup Foundation of Northeast Agricultural University(LBH-Z10266)+2 种基金New Rural Project of Heilongjiang Provincial Department of EducationProject of Education Department in Heilongjiang Province(12521035)Postgraduate Innovation Project of Education Department in Heilongjiang Province (YJSCX2012-036HLJ)
文摘Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water composition and content, and the changes in cell viability in the crowns, which contain the growing point, were studied during the period from cold acclimation to the deep freezing. The results showed that electrical conductivity which reflects the degree of plasma membrane damaged under low temperature was significantly correlated with the free water and the total water content. The malondialdehyde （MDA） content, which reflects the degree of membrane peroxidation, was very significantly correlated with superoxide dismutase （SOD）, peroxidase, and ascorbic acid. During the deep freezing period, the SOD activity and glutathione （GSH） content of the winter wheat varieties were relative to their cold resistance. During this period, the MDA stability, SOD, GSH, and the total water and the free water content might be used to identify the cold resistance of winter wheat varieties.
