Object To elucidate the physiological significance of the spiral flow in the arterial system from the viewpoint of atherogenic lipid transport,an ex vivo experimental comparative
Objective To investigate the structural changes of rat thoracic aorta and changes in expression levels of Bmal1 and cyclins in thoracic aorta endothelial cells following heat stress.Methods Twenty male SD rats were ra...Objective To investigate the structural changes of rat thoracic aorta and changes in expression levels of Bmal1 and cyclins in thoracic aorta endothelial cells following heat stress.Methods Twenty male SD rats were randomized equally into control group and heat stress group.After exposure to 32℃for 2 weeks in the latter group,the rats were examined for histopathological changes and Bmal1 expression in the thoracic aorta using HE staining and immunohistochemistry.In the cell experiments,cultured rat thoracic aortic endothelial cells(RTAECs)were incubated at 40℃for 12 h with or without prior transfection with a Bmal1-specific small interfering RNA(si-Bmal1)or a negative sequence.In both rat thoracic aorta and RTAECs,the expressions of Bmal1,the cell cycle proteins CDK1,CDK4,CDK6,and cyclin B1,and apoptosis-related proteins Bax and Bcl-2 were detected using Western blotting.TUNEL staining was used to detect cell apoptosis in rat thoracic aorta,and the changes in cell cycle distribution and apoptosis in RTAECs were analyzed with flow cytometry.Results Compared with the control rats,the rats exposed to heat stress showed significantly increased blood pressures and lowered heart rate with elastic fiber disruption and increased expressions of Bmal1,cyclin B1 and CDK1 in the thoracic aorta(P<0.05).In cultured RTAECs,heat stress caused significant increase of Bmal1,cyclin B1 and CDK1 protein expression levels,which were obviously lowered in cells with prior si-Bmal1 transfection.Bmal1 knockdown also inhibited heat stress-induced increase of apoptosis in RTAECs as evidenced by decreased expression of Bax and increased expression of Bcl-2.Conclusion Heat stress upregulates Bmal1 expression and causes alterations in expressions of cyclins to trigger apoptosis of rat thoracic aorta endothelial cells,which can be partly alleviated by suppressing Bmal1 expression.展开更多
Objective:To investigate the correlation of abdominal aorta CT value,renal artery CT value and renal cortex thickness with renal cortex CT value on contrast enhanced 64-slice CT images.Methods:96patients(50 men and 46...Objective:To investigate the correlation of abdominal aorta CT value,renal artery CT value and renal cortex thickness with renal cortex CT value on contrast enhanced 64-slice CT images.Methods:96patients(50 men and 46women;16~74years)with normal kidney function,which was confirmed by kidney function test were enrolled in this study,including bilateral kidneys of 92cases and unilateral kidney of 4cases(total of 188kidneys;92left,96right).After intravenous(IV)injection of contrast agent the kidneys of the selected patients were scanned by MDCT.The scans were performed in arterial,venous and 3min delayed phases.All statistical analyses were performed by using IBM SPSS 20.0.Graphs were generated using Graph Pad Prism 5software.Quantitative data were presented as mean±standard deviation,while qualitative data were presented as frequency(%).P<0.05was considered to be statistically significant.Results:The mean renal cortex thickness was(5.19±0.81)mm in all kidneys.In the arterial phase,a statistically significant positive correlation between renal cortex CT values and abdominal aortic CT values was showed(r=0.584;P<0.001).A statistically significant positive correlation between renal cortex CT values and renal cortex thickness was demonstrated(r=0.533,P<0.0001).Likewise,there was a positive correlation between renal cortex CT value and renal artery CT values(r=0.43,P<0.001).Conclusion:It is a promising approach to assess the individual kidney function by measuring abdominal aorta CT value,renal artery CT value,renal cortex CT value and renal cortex thickness using contrast MDCT.展开更多
It has been suggested that hypoxia may occur in the arterial system and is likely to involve in the localization of atherogenesis[1].In the present communication,we tested the hypothesis numerically that the spiral fl...It has been suggested that hypoxia may occur in the arterial system and is likely to involve in the localization of atherogenesis[1].In the present communication,we tested the hypothesis numerically that the spiral flow observed in the aorta may have a great impact on oxygen展开更多
Objective Acute Stanford type A aortic dissection is typically presented as antegrade dissection from a primary intimal tear in the proximal ascending aorta extending to the arch and the downstream distal aorta.Howeve...Objective Acute Stanford type A aortic dissection is typically presented as antegrade dissection from a primary intimal tear in the proximal ascending aorta extending to the arch and the downstream distal aorta.However,it may also develop in a retrograde fashion with an intimal tear located in descending aorta causing retrograde extension of aortic dissection into ascending aorta,namely,retrograde type A aortic dissection(RTAAD).Though open surgery remains the golden standard to treat type A dissection,as to RTAAD,the distal location of its primary entry tear in descending aorta warrants more extensive aortic repair with higher surgical risks of mortality or morbidity through a median sternotomy.Conversely,confining the surgical extent to the proximal aorta leaves the risks of complications related to the residual tear in the distal aorta untreated.More recently,thoracic endovascular aortic repair(TEVAR)is attempted for the treatment of RTAAD with favorable shortterm and long-term prognosis.To the best of our knowledge,this was the largest serial study to apply TEVAR in the treatment of RTAAD.展开更多
目的:回顾性分析脊柱胸椎前凸患者及正常人群术前影像学及超声心动图资料,探讨并分析胸椎前凸与患者心脏结构及功能的关系。方法:收集并分析2013年1月~2023年12月期间胸椎前凸患者和正常人群的影像学及超声心动图资料。根据胸椎角度将...目的:回顾性分析脊柱胸椎前凸患者及正常人群术前影像学及超声心动图资料,探讨并分析胸椎前凸与患者心脏结构及功能的关系。方法:收集并分析2013年1月~2023年12月期间胸椎前凸患者和正常人群的影像学及超声心动图资料。根据胸椎角度将纳入患者分为两组,A组为胸椎前凸(TL)组(T5-T12≤0°),27例;B组为胸椎后凸(TK)减小组(0°<T5-T12≤20°),29例;另设正常TK(20°<T5-T12≤40°)的C组为对照组,29例。收集三组人群术前临床、影像学及超声心动图资料,包括一般资料[性别、年龄、身高、体重、体重指数(BMI)、体表面积(BSA)],X线片影像学资料(胸椎冠、矢状面Cobb角),顶椎区CT影像学资料[脊柱穿透指数(spinal penetration index volume,SPIV)、顶椎区心脏容积比(cardiac volume ratio in apical vertebra region,CVRA)],超声心动图资料[左心室舒张末期内径(LVDd)、右心室舒张末期内径(RVDd)、左心房内径(LAD)、右心房内径(RAD)、右心室流出道(RVOT)、主动脉内径(AO)、升主动脉内径(AAO)、主肺动脉内径(MPA)、舒张末期室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、射血分数(EF)、缩短分数(FS)、每搏输出量(SV)、心脏输出量(CO)、心脏指数(CI)、肺动脉收缩压(SPAP)]。采用SPSS 25.0统计分析软件对三组人群的上述资料进行统计、比较及相关性分析。结果:SPIV与矢状面Cobb角负相关,与冠状面Cobb角无相关性;SPIV与LVDd、RVDd、LAD、RAD、RVOT、AO、AAO、MPA、IVST、LVPWT呈负相关;SPIV与EF、FS、SV、CO、CI、SPAP无相关性;CVRA与矢状面Cobb角负相关,与冠状面Cobb角无相关性;CVRA与RVDd、IVST、LVPWT负相关;CVRA与LVDd、LAD、RAD、RVOT、AO、AAO、MPA之间无相关性;CVRA与EF、FS、SV、CO、CI、SPAP无相关性。结论:SPIV较CVRA更能反映胸椎前凸对心脏结构和功能的影响,胸椎后凸角度的减小可能加剧心脏血管负担。在胸椎前凸患者矫形手术过程中,由于全麻、俯卧位、胸骨及胸廓受压和肌松药物等因素,胸腔和心腔结构及功能参数可能发生显著变化,应特别关注气道阻塞和血流动力学不稳定的风险。展开更多
基金supported by Grants-in-Aid from the National Natural Science Research Foundation of China,No.30670517,10632010
文摘Object To elucidate the physiological significance of the spiral flow in the arterial system from the viewpoint of atherogenic lipid transport,an ex vivo experimental comparative
文摘Objective To investigate the structural changes of rat thoracic aorta and changes in expression levels of Bmal1 and cyclins in thoracic aorta endothelial cells following heat stress.Methods Twenty male SD rats were randomized equally into control group and heat stress group.After exposure to 32℃for 2 weeks in the latter group,the rats were examined for histopathological changes and Bmal1 expression in the thoracic aorta using HE staining and immunohistochemistry.In the cell experiments,cultured rat thoracic aortic endothelial cells(RTAECs)were incubated at 40℃for 12 h with or without prior transfection with a Bmal1-specific small interfering RNA(si-Bmal1)or a negative sequence.In both rat thoracic aorta and RTAECs,the expressions of Bmal1,the cell cycle proteins CDK1,CDK4,CDK6,and cyclin B1,and apoptosis-related proteins Bax and Bcl-2 were detected using Western blotting.TUNEL staining was used to detect cell apoptosis in rat thoracic aorta,and the changes in cell cycle distribution and apoptosis in RTAECs were analyzed with flow cytometry.Results Compared with the control rats,the rats exposed to heat stress showed significantly increased blood pressures and lowered heart rate with elastic fiber disruption and increased expressions of Bmal1,cyclin B1 and CDK1 in the thoracic aorta(P<0.05).In cultured RTAECs,heat stress caused significant increase of Bmal1,cyclin B1 and CDK1 protein expression levels,which were obviously lowered in cells with prior si-Bmal1 transfection.Bmal1 knockdown also inhibited heat stress-induced increase of apoptosis in RTAECs as evidenced by decreased expression of Bax and increased expression of Bcl-2.Conclusion Heat stress upregulates Bmal1 expression and causes alterations in expressions of cyclins to trigger apoptosis of rat thoracic aorta endothelial cells,which can be partly alleviated by suppressing Bmal1 expression.
文摘Objective:To investigate the correlation of abdominal aorta CT value,renal artery CT value and renal cortex thickness with renal cortex CT value on contrast enhanced 64-slice CT images.Methods:96patients(50 men and 46women;16~74years)with normal kidney function,which was confirmed by kidney function test were enrolled in this study,including bilateral kidneys of 92cases and unilateral kidney of 4cases(total of 188kidneys;92left,96right).After intravenous(IV)injection of contrast agent the kidneys of the selected patients were scanned by MDCT.The scans were performed in arterial,venous and 3min delayed phases.All statistical analyses were performed by using IBM SPSS 20.0.Graphs were generated using Graph Pad Prism 5software.Quantitative data were presented as mean±standard deviation,while qualitative data were presented as frequency(%).P<0.05was considered to be statistically significant.Results:The mean renal cortex thickness was(5.19±0.81)mm in all kidneys.In the arterial phase,a statistically significant positive correlation between renal cortex CT values and abdominal aortic CT values was showed(r=0.584;P<0.001).A statistically significant positive correlation between renal cortex CT values and renal cortex thickness was demonstrated(r=0.533,P<0.0001).Likewise,there was a positive correlation between renal cortex CT value and renal artery CT values(r=0.43,P<0.001).Conclusion:It is a promising approach to assess the individual kidney function by measuring abdominal aorta CT value,renal artery CT value,renal cortex CT value and renal cortex thickness using contrast MDCT.
基金supported by Grants-in-Aid from the National Natural Science Foundation of China,No.10632010,30670517
文摘It has been suggested that hypoxia may occur in the arterial system and is likely to involve in the localization of atherogenesis[1].In the present communication,we tested the hypothesis numerically that the spiral flow observed in the aorta may have a great impact on oxygen
文摘Objective Acute Stanford type A aortic dissection is typically presented as antegrade dissection from a primary intimal tear in the proximal ascending aorta extending to the arch and the downstream distal aorta.However,it may also develop in a retrograde fashion with an intimal tear located in descending aorta causing retrograde extension of aortic dissection into ascending aorta,namely,retrograde type A aortic dissection(RTAAD).Though open surgery remains the golden standard to treat type A dissection,as to RTAAD,the distal location of its primary entry tear in descending aorta warrants more extensive aortic repair with higher surgical risks of mortality or morbidity through a median sternotomy.Conversely,confining the surgical extent to the proximal aorta leaves the risks of complications related to the residual tear in the distal aorta untreated.More recently,thoracic endovascular aortic repair(TEVAR)is attempted for the treatment of RTAAD with favorable shortterm and long-term prognosis.To the best of our knowledge,this was the largest serial study to apply TEVAR in the treatment of RTAAD.
文摘目的:回顾性分析脊柱胸椎前凸患者及正常人群术前影像学及超声心动图资料,探讨并分析胸椎前凸与患者心脏结构及功能的关系。方法:收集并分析2013年1月~2023年12月期间胸椎前凸患者和正常人群的影像学及超声心动图资料。根据胸椎角度将纳入患者分为两组,A组为胸椎前凸(TL)组(T5-T12≤0°),27例;B组为胸椎后凸(TK)减小组(0°<T5-T12≤20°),29例;另设正常TK(20°<T5-T12≤40°)的C组为对照组,29例。收集三组人群术前临床、影像学及超声心动图资料,包括一般资料[性别、年龄、身高、体重、体重指数(BMI)、体表面积(BSA)],X线片影像学资料(胸椎冠、矢状面Cobb角),顶椎区CT影像学资料[脊柱穿透指数(spinal penetration index volume,SPIV)、顶椎区心脏容积比(cardiac volume ratio in apical vertebra region,CVRA)],超声心动图资料[左心室舒张末期内径(LVDd)、右心室舒张末期内径(RVDd)、左心房内径(LAD)、右心房内径(RAD)、右心室流出道(RVOT)、主动脉内径(AO)、升主动脉内径(AAO)、主肺动脉内径(MPA)、舒张末期室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、射血分数(EF)、缩短分数(FS)、每搏输出量(SV)、心脏输出量(CO)、心脏指数(CI)、肺动脉收缩压(SPAP)]。采用SPSS 25.0统计分析软件对三组人群的上述资料进行统计、比较及相关性分析。结果:SPIV与矢状面Cobb角负相关,与冠状面Cobb角无相关性;SPIV与LVDd、RVDd、LAD、RAD、RVOT、AO、AAO、MPA、IVST、LVPWT呈负相关;SPIV与EF、FS、SV、CO、CI、SPAP无相关性;CVRA与矢状面Cobb角负相关,与冠状面Cobb角无相关性;CVRA与RVDd、IVST、LVPWT负相关;CVRA与LVDd、LAD、RAD、RVOT、AO、AAO、MPA之间无相关性;CVRA与EF、FS、SV、CO、CI、SPAP无相关性。结论:SPIV较CVRA更能反映胸椎前凸对心脏结构和功能的影响,胸椎后凸角度的减小可能加剧心脏血管负担。在胸椎前凸患者矫形手术过程中,由于全麻、俯卧位、胸骨及胸廓受压和肌松药物等因素,胸腔和心腔结构及功能参数可能发生显著变化,应特别关注气道阻塞和血流动力学不稳定的风险。
