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Micro LED表面缺陷的快速高精度检测 被引量:2
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作者 赵天元 董登峰 +2 位作者 王国名 王博 周维虎 《光学精密工程》 北大核心 2025年第9期1434-1445,共12页
为了满足Micro LED缺陷检测对实时性和高精度的要求,结合轻量化的设计和增强的特征提取能力,提出了一种快速高精度的检测算法LED-YOLO。该方法通过设计图像采集系统并结合多种数据增广策略,模拟工业环境中的实际干扰,增强了训练数据的... 为了满足Micro LED缺陷检测对实时性和高精度的要求,结合轻量化的设计和增强的特征提取能力,提出了一种快速高精度的检测算法LED-YOLO。该方法通过设计图像采集系统并结合多种数据增广策略,模拟工业环境中的实际干扰,增强了训练数据的多样性。针对Micro LED缺陷的低区分度问题,提出了轻量级动态融合模块(Lightweight Dynamic Fusion Module,LDFM),该模块结合动态卷积、深度卷积和通道混合操作,在保持模型轻量化的同时,提升了特征表达能力。为了进一步加强缺陷区域的关注,设计了增强协调注意力模块(Enhanced Coordinate Attention Module,ECAM),通过结合通道和空间注意力机制及残差连接,增强了特征提取的准确性。最后,考虑到Micro LED图像的纵横比变化较小,引入动态聚焦机制,提出了DIoU_W回归损失函数,显著提高了模型的收敛速度和稳定性。实验结果表明,LED-YOLO的检测准确率、召回率、mAP、F1值均优于目前最先进的YOLOv11s模型,在参数量减少1.6 M的情况下,检测速度和检测精度均有明显提升,可以有效满足实际Micro LED面板制造过程的质量检验需求。 展开更多
关键词 深度学习 micro LED 缺陷检测 动态卷积 注意力机制
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基于量子点颜色转换层的Micro LED研究进展
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作者 程旭东 陈祖康 +3 位作者 张针霖 朱艳青 徐刚 徐雪青 《新能源进展》 北大核心 2025年第1期107-120,共14页
微发光二极管(Micro LED)显示器是由微米级半导体发光像元阵列所组成的新型显示技术,是显示技术与LED技术复合集成的综合性技术。与液晶显示器和有机发光二极管显示器相比,Micro LED具有对比度高、功耗低、寿命长、响应时间短等优点。然... 微发光二极管(Micro LED)显示器是由微米级半导体发光像元阵列所组成的新型显示技术,是显示技术与LED技术复合集成的综合性技术。与液晶显示器和有机发光二极管显示器相比,Micro LED具有对比度高、功耗低、寿命长、响应时间短等优点。然而,由于LED芯片尺寸缩小至20μm以下,导致其吸收截面减小,使得传统的荧光粉颜色转换技术无法提供足够的亮度和产量,以满足高分辨率显示的需求。而量子点材料凭借其高量子产率、宽色域、颜色可调等优点,有望成为代替荧光粉的最佳材料。结合了量子点颜色转换技术的Micro LED光电器件具有高亮度、高效率和宽色域的优势,在显示领域具有广阔的应用前景。目前许多学界和产业界的研究者对全彩显示的Micro LED进行了深入研究,逐步实现了Micro LED的商业化。简要回顾了广泛应用于显示领域的量子点材料合成和优异性能的重要研究成果,然后以印刷技术、光刻技术、微流控技术、激光写入技术这四种效果突出的颜色转换层沉积工艺分类总结了基于量子点颜色转换技术Micro LED的全彩显示策略与性能优劣。最后,对基于量子点颜色转换层的Micro LED光电器件的应用前景进行了展望。 展开更多
关键词 量子点 micro LED 颜色转换层 全彩显示
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Numerical and experimental investigation on the formability of stainless steel-copper composites during micro deep drawing
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作者 QI Yan-yang MA Xiao-guang +6 位作者 JIANG Zheng-yi MA Li-nan WANG Zhi-hua ZHOU Cun-long HASAN Mahadi DOBRZAŃSKI Leszek A. ZHAO Jing-wei 《Journal of Central South University》 2025年第4期1237-1251,共15页
In the present study,two-layered stainless steel-copper composites with a thickness of 50μm were initially subjected to annealing at 800,900 and 1000℃for 5 min,respectively,to achieve diverse microstructural feature... In the present study,two-layered stainless steel-copper composites with a thickness of 50μm were initially subjected to annealing at 800,900 and 1000℃for 5 min,respectively,to achieve diverse microstructural features.Then the influence of annealing temperature on the formability of stainless steel-copper composites and the quality of micro composite cups manufactured by micro deep drawing(MDD)were investigated,and the underlying mechanism was analyzed.Three finite element(FE)models,including basic FE model,Voronoi FE model and surface morphological FE model,were developed to analyze the forming performance of stainless steel-copper composites during MDD.The results show that the stainless steel-copper composites annealed at 900℃possess the best plasticity owing to the homogeneous and refined microstructure in both stainless steel and copper matrixes,and the micro composite cup with specimen annealed at 900℃exhibits a uniform wall thickness as well as high surface quality with the fewest wrinkles.The results obtained from the surface morphological FE model considering material inhomogeneity and surface morphology of the composites are the closest to the experimental results compared to the basic and Voronoi FE model.During MDD process,the drawing forces decrease with increasing annealing temperature as a consequence of the strength reduction. 展开更多
关键词 micro deep drawing annealing temperature stainless steel-copper composites FORMABILITY WRINKLING finite element method
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Designing of optimized microstrip fractal antenna using hybrid metaheuristic framework for IoT applications
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作者 S KARUNAKAR Reddy ANITHA Guttavelli 《Journal of Systems Engineering and Electronics》 2025年第3期659-670,共12页
Nowadays,wireless communication devices turn out to be transportable owing to the execution of the current technologies.The antenna is the most important component deployed for communication purposes.The antenna plays... Nowadays,wireless communication devices turn out to be transportable owing to the execution of the current technologies.The antenna is the most important component deployed for communication purposes.The antenna plays an imperative role in receiving and transmitting the signals for any sensor network.Among varied antennas,micro strip fractal antenna(MFA)significantly contributes to increasing antenna gain.This study employs a hybrid optimization method known as the elephant clan updated grey wolf algorithm to introduce an optimized MFA design.This method optimizes antenna characteristics,including directivity and gain.Here,the factors,including length,width,ground plane length,height,and feed offset-X and feed offset-Y,are taken into account to achieve the best performance of gain and directivity.Ultimately,the superiority of the suggested technique over state-of-the-art strategies is calculated for various metrics such as cost and gain.The adopted model converges to a minimal value of 0.2872.Further,the spider monkey optimization(SMO)model accomplishes the worst performance over all other existing models like elephant herding optimization(EHO),grey wolf optimization(GWO),lion algorithm(LA),support vector regressor(SVR),bacterial foraging-particle swarm optimization(BF-PSO)and shark smell optimization(SSO).Effective MFA design is obtained using the suggested strategy regarding various parameters. 展开更多
关键词 micro strip fractal antenna(MFA)model gain DIRECTIVITY support vector regressor(SVR)approach elephant clan updated grey wolf algorithm(ECU-GWA)
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Micro LED车灯投影光学系统设计与优化 被引量:4
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作者 李香兰 金霞 +7 位作者 吕金光 郑凯丰 陈宇鹏 赵百轩 赵莹泽 秦余欣 王惟彪 梁静秋 《中国光学(中英文)》 EI CAS CSCD 北大核心 2024年第1期89-99,共11页
本文提出了一种基于Micro LED阵列的车灯投影方案,设计了以像素尺寸为80μm×80μm的200×150白光Micro LED阵列作为显示光源,视场角为16°×34°的车灯投影光学系统,并对物面倾斜角度和光学系统结构进行了优化。此... 本文提出了一种基于Micro LED阵列的车灯投影方案,设计了以像素尺寸为80μm×80μm的200×150白光Micro LED阵列作为显示光源,视场角为16°×34°的车灯投影光学系统,并对物面倾斜角度和光学系统结构进行了优化。此外,分别采用反向畸变处理方法和像素灰度调制方法用以解决车灯投影图像的梯形畸变和照度均匀性问题,并搭建了投影实验平台,对图像校正方法进行了验证。实验结果表明:校正后图像梯形畸变系数p1,p2分别从0.0932和0.3680下降至0.0835和0.0373,像面照度均匀性从83.2%提高到93.2%。本文通过对基于Micro LED的倾斜投影车灯光学系统进行优化设计及采用图像校正方法,实现了高光效、低畸变的车灯投影。 展开更多
关键词 车灯投影光学系统 光学设计 micro LED 照度均匀性 梯形畸变
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光子晶体Micro LED微显示阵列加工及光学特性分析 被引量:2
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作者 孟媛 肖秧 +4 位作者 冯晓雨 何龙振 张鹏喆 宁平凡 刘宏伟 《半导体技术》 CAS 北大核心 2024年第8期719-725,共7页
Micro LED器件具有高亮度、低功耗和高可靠性等优点,但Micro LED显示像素巨量转移和光提取效率低的问题为其应用带来挑战。开发了具有高转移效率和出光效率的单片64×64 Micro LED微显示阵列,提出了倒装型GaN基单片Micro LED微显示... Micro LED器件具有高亮度、低功耗和高可靠性等优点,但Micro LED显示像素巨量转移和光提取效率低的问题为其应用带来挑战。开发了具有高转移效率和出光效率的单片64×64 Micro LED微显示阵列,提出了倒装型GaN基单片Micro LED微显示阵列芯片和Si基驱动电路的设计方法及集成工艺。通过时域有限差分(FDTD)方法对Micro LED微显示阵列光学特性进行了建模分析,设计了一种提高Micro LED微显示阵列出光效率的光提取结构。结合仿真结果,开发了一种在Micro LED蓝宝石衬底表面制备光子晶体结构的聚焦离子束(FIB)微纳加工工艺,并进行了器件加工。测试结果表明,蓝宝石衬底上加工的光子晶体结构可以提高Micro LED器件的表面出光效率,光功率平均值提升了16.36%,对Micro LED微显示阵列加工及微显示像素光提取问题具有借鉴意义。 展开更多
关键词 micro LED 微显示阵列 光子晶体结构 聚焦离子束(FIB) 出光效率
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基于Micro CT的铜导线短路熔痕孔洞特征分析 被引量:1
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作者 陈克 郭宇航 +3 位作者 邓松华 王轩磊 张亮亮 张斌 《消防科学与技术》 CAS 北大核心 2024年第4期566-571,共6页
导线短路是造成电气火灾的重要原因之一。现行国家标准中将导线短路熔痕分为起火前发生的一次短路熔痕和起火后造成的二次短路熔痕,然而现行国家标准中的鉴定仅停留在定性判断的阶段。火灾现场铜导线短路熔痕特征的量化分析研究是国内... 导线短路是造成电气火灾的重要原因之一。现行国家标准中将导线短路熔痕分为起火前发生的一次短路熔痕和起火后造成的二次短路熔痕,然而现行国家标准中的鉴定仅停留在定性判断的阶段。火灾现场铜导线短路熔痕特征的量化分析研究是国内外研究人员主要关注的重点。本文应用Micro CT技术对铜导线短路熔痕进行断层扫描检测并重构铜导线短路熔痕的3D图像数据,在此基础上统计、归纳、总结铜导线短路熔痕内部孔洞形态及分布等孔洞特征。研究表明,Micro CT能够全面采集铜导线短路熔痕的整体形态和内部孔洞特征,一次短路熔痕与二次短路熔痕内部孔洞半径、表面积、紧密度的特征数据存在差别,可为短路熔痕定性和定量分析判据研究提供新的理论依据。 展开更多
关键词 micro CT 短路熔痕 孔洞特征 火灾物证鉴定
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Macroscopic and microscopic mechanical behavior and seepage characteristics of coal under hydro-mechanical coupling 被引量:1
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作者 GAO Ming-zhong GAO Zheng +6 位作者 YANG Ben-gao XIE Jing WANG Ming-yao HAO Hai-chun WU Yan ZHOU Lang WANG Jing-yu 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第8期2765-2779,共15页
Understanding the physical,mechanical behavior,and seepage characteristics of coal under hydro-mechanical coupling holds significant importance for ensuring the stability of surrounding rock formations and preventing ... Understanding the physical,mechanical behavior,and seepage characteristics of coal under hydro-mechanical coupling holds significant importance for ensuring the stability of surrounding rock formations and preventing gas outbursts.Scanning electron microscopy,uniaxial tests,and triaxial tests were conducted to comprehensively analyze the macroscopic and microscopic physical and mechanical characteristics of coal under different soaking times.Moreover,by restoring the stress path and water injection conditions of the protective layer indoors,we explored the coal mining dynamic behavior and the evolution of permeability.The results show that water causes the micro-surface of coal to peel off and cracks to expand and develop.With the increase of soaking time,the uniaxial and triaxial strengths were gradually decreased with nonlinear trend,and decreased by 63.31%and 30.95%after soaking for 240 h,respectively.Under different water injection pressure conditions,coal permeability undergoes three stages during the mining loading process and ultimately increases to higher values.The peak stress of coal,the deviatoric stress and strain at the permeability surge point all decrease with increasing water injection pressure.The results of this research can help improve the understanding of the coal mechanical properties and seepage evolution law under hydro-mechanical coupling. 展开更多
关键词 COAL water intrusion mechanical properties PERMEABILITY macro and micro features
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Microscopic defects formation and dynamic mechanical response analysis of Q345 steel plate subjected to explosive load 被引量:1
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作者 Zhengqing Zhou Zechen Du +6 位作者 Yulong Zhang Guili Yang Ruixiang Wang Yuzhe Liu Peize Zhang Yaxin Zhang Xiao Wang 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第2期430-442,共13页
As the basic protective element, steel plate had attracted world-wide attention because of frequent threats of explosive loads. This paper reports the relationships between microscopic defects of Q345 steel plate unde... As the basic protective element, steel plate had attracted world-wide attention because of frequent threats of explosive loads. This paper reports the relationships between microscopic defects of Q345 steel plate under the explosive load and its macroscopic dynamics simulation. Firstly, the defect characteristics of the steel plate were investigated by stereoscopic microscope(SM) and scanning electron microscope(SEM). At the macroscopic level, the defect was the formation of cave which was concentrated in the range of 0-3.0 cm from the explosion center, while at the microscopic level, the cavity and void formation were the typical damage characteristics. It also explains that the difference in defect morphology at different positions was the combining results of high temperature and high pressure. Secondly, the variation rules of mechanical properties of steel plate under explosive load were studied. The Arbitrary Lagrange-Euler(ALE) algorithm and multi-material fluid-structure coupling method were used to simulate the explosion process of steel plate. The accuracy of the method was verified by comparing the deformation of the simulation results with the experimental results, the pressure and stress at different positions on the surface of the steel plate were obtained. The simulation results indicated that the critical pressure causing the plate defects may be approximately 2.01 GPa. On this basis, it was found that the variation rules of surface pressure and microscopic defect area of the Q345 steel plate were strikingly similar, and the corresponding mathematical relationship between them was established. Compared with Monomolecular growth fitting models(MGFM) and Logistic fitting models(LFM), the relationship can be better expressed by cubic polynomial fitting model(CPFM). This paper illustrated that the explosive defect characteristics of metal plate at the microscopic level can be explored by analyzing its macroscopic dynamic mechanical response. 展开更多
关键词 Explosive load Q345 steel micro defect Finite element simulation Dynamic response Data fitting
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Micro defects formation and dynamic response analysis of steel plate of quasi-cracking area subjected to explosive load
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作者 Zheng-qing Zhou Ze-chen Du +5 位作者 Xiao Wang Hui-ling Jiang Qiang Zhou Yu-long Zhang Yu-zhe Liu Pei-ze Zhang 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第1期580-593,共14页
As the protective component,steel plate had attracted extensive attention because of frequently threats of explosive loads.In this paper,the evolution of microstructure and the mechanism of damage in the quasi-crackin... As the protective component,steel plate had attracted extensive attention because of frequently threats of explosive loads.In this paper,the evolution of microstructure and the mechanism of damage in the quasi-cracking area of steel plate subjected to explosive load were discussed and the relationships between micro defects and dynamic mechanical response were revealed.After the explosion experiment,five observation points were selected equidistant from the quasi-cracking area of the section of the steel plate along the thickness direction,and the characteristics of micro defects at the observation points were analyzed by optical microscope(OM),scanning electron microscope(SEM) and electron backscattered diffraction(EBSD).The observation result shows that many slip bands(SBs) appeared,and the grain orientation changed obviously in the steel plate,the two were the main damage types of micro defects.In addition,cracks,peeling pits,grooves and other lager micro defects were appeared in the lower area of the plate.The stress parameters of the observation points were obtained through an effective numerical model.The mechanism of damage generation and crack propagation in the quasicracking area were clarified by comparing the specific impulse of each observation point with the corresponding micro defects.The result shows that the generation and expansion of micro defects are related to the stress area(i.e.the upper compression area,the neutral plane area,and the lower tension area).The micro defects gather and expand at the grain boundary,and will become macroscopic damage under the continuous action of tensile stress.Besides,the micro defects at the midpoint of the section of the steel plate in the direction away from the explosion center(i.e.the horizontal direction) were also studied.It was found that the specific impulse at these positions were much smaller than that in the thickness direction,the micro defects were only SBs and a few micro cracks,and the those decreased with the increase of the distance from the explosion center. 展开更多
关键词 Explosive load Quasi-cracking area micro defects Steel plate Dynamic response Numerical simulation
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Ignition processes and characteristics of charring conductive polymers with a cavity geometry in precombustion chamber for applications in micro/nano satellite hybrid rocket motors
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作者 Zhiyuan Zhang Hanyu Deng +2 位作者 Wenhe Liao Bin Yu Zai Yu 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第2期55-66,共12页
The arc ignition system based on charring polymers has advantages of simple structure,low ignition power consumption and multiple ignitions,which bringing it broadly application prospect in hybrid propulsion system of... The arc ignition system based on charring polymers has advantages of simple structure,low ignition power consumption and multiple ignitions,which bringing it broadly application prospect in hybrid propulsion system of micro/nano satellite.However,charring polymers alone need a relatively high input voltage to achieve pyrolysis and ignition,which increases the burden and cost of the power system of micro/nano satellite in practical application.Adding conductive substance into charring polymers can effectively decrease the conducting voltage which can realize low voltage and low power consumption repeated ignition of arc ignition system.In this paper,a charring conductive polymer ignition grain with a cavity geometry in precombustion chamber,which is composed of PLA and multiwall carbon nanotubes(MWCNT)was proposed.The detailed ignition processes were analyzed and two different ignition mechanisms in the cavity of charring conductive polymers were revealed.The ignition characteristics of charring conductive polymers were also investigated at different input voltages,ignition grain structures,ignition locations and injection schemes in a visual ignition combustor.The results demonstrated that the ignition delay and external energy required for ignition were inversely correlated with the voltages applied to ignition grain.Moreover,the incremental depth of cavity shortened the ignition delay and external energy required for ignition while accelerated the propagation of flame.As the depth of cavity increased from 2 to 6 mm(at 50 V),the time of flame propagating out of ignition grain changed from 235.6 to 108 ms,and values of mean ignition delay time and mean external energy required for ignition decreased from 462.8 to 320 ms and 16.2 to 10.75 J,respectively.The rear side of the cavity was the ideal ignition position which had a shorter ignition delay and a faster flame propagation speed in comparison to other ignition positions.Compared to direct injection scheme,swirling injection provided a more favorable flow field environment in the cavity,which was beneficial to ignition and initial flame propagation,but the ignition position needed to be away from the outlet of swirling injector.At last,the repeated ignition characteristic of charring conductive polymers was also investigated.The ignition delay time and external energy required for ignition decreased with repeated ignition times but the variation was decreasing gradually. 展开更多
关键词 micro/nano satellite hybrid propulsion Arc ignition Charring conductive polymer Ignition mechanism Ignition characteristic Repeated ignition
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气动巨量转移机构刺晶减振控制 被引量:1
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作者 刘强 樊竞超 +2 位作者 牛萍娟 陈云喆 孙海威 《振动与冲击》 北大核心 2025年第11期157-162,共6页
针对气动针刺式巨量转机构在高速、高频刺晶过程横梁扭转振动影响转移精度问题,提出了一种基于动态平衡法的双边侧挂直线电机主动减振方案,基于同步反向运动的针刺系统和随动系统,建立了横梁扭转振动幅值的数学模型,并使用有限元法对运... 针对气动针刺式巨量转机构在高速、高频刺晶过程横梁扭转振动影响转移精度问题,提出了一种基于动态平衡法的双边侧挂直线电机主动减振方案,基于同步反向运动的针刺系统和随动系统,建立了横梁扭转振动幅值的数学模型,并使用有限元法对运动过程进行瞬态分析。直线电机电流控制上采用了BP-PID(back propagation-process identifier)方法调控参数,提高了电流函数收敛速度,降低了启动电流超调量,优化了电机推力的控制方法。试验表明,在不间断加速模式与高速启停模式下分别减小横梁振幅86.74%与84.47%,证明了动态平衡法主动减振结构对抑制气动针刺巨量转移装置横梁扭转振动效果明显,对巨量转移精度提升具有重要意义。 展开更多
关键词 巨量转移 Mini/micro LED 主动减振 有限元
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基于micro CT扫描技术的鲤骨骼和显微结构分析 被引量:8
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作者 张宁 苏胜彦 +2 位作者 董在杰 马庆男 袁新华 《南方水产科学》 CAS 北大核心 2012年第6期44-49,共6页
了解活体鲤(Cyprinus carpio)骨结构、形态、连接、空间分布以及骨骼矿物质组成对鲤的选育、行为学研究以及肉品加工有重要的意义。X射线显微CT技术(micro CT)对样品没有破坏,可实现活体检测,并能获取活体任意形态的结构参数。因此,文... 了解活体鲤(Cyprinus carpio)骨结构、形态、连接、空间分布以及骨骼矿物质组成对鲤的选育、行为学研究以及肉品加工有重要的意义。X射线显微CT技术(micro CT)对样品没有破坏,可实现活体检测,并能获取活体任意形态的结构参数。因此,文章通过这项技术来研究活体鲤的骨骼状况。结果显示,脊椎骨与头、尾相连,胸鳍位于头骨后,背鳍、腹鳍和尾鳍与整个脊椎骨并不相连。骨体积分数和骨小梁厚度在腹鳍较高,头尾较低;骨表面积体积比、骨小梁数量和骨小梁间隔在头部较高,腹鳍较低;骨矿物质含量、骨矿物质密度和组织矿物质含量在背鳍最高;骨体积分数在腹鳍最高,胸鳍、背鳍和尾鳍次之,头尾较低。骨小梁厚度、矿物质含量等在背鳍、腹鳍部位较高,这可能与鲤的运动有关,而头骨表面积体积比、骨小梁数量和骨小梁间隔较高可能与头部的复杂结构密切相关。 展开更多
关键词 micro CT 显微结构
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microRNA let-7a对人晶状体上皮细胞凋亡的调控及其在白内障中的作用 被引量:7
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作者 秦宇 赵江月 +6 位作者 闵晓洁 罗文婷 李晶 吴欣蔚 刘佳 阎启昌 张劲松 《中国医科大学学报》 CAS CSCD 北大核心 2015年第4期302-306,共5页
目的探讨micro RNA let-7a对人晶状体上皮细胞凋亡的调控作用及其在白内障中的作用。方法利用Real time q-PCR方法,检测年龄相关性白内障患者晶状体前囊膜和正常人眼晶状体前囊膜、人晶状体上皮细胞凋亡模型和正常人晶状体上皮细胞系let... 目的探讨micro RNA let-7a对人晶状体上皮细胞凋亡的调控作用及其在白内障中的作用。方法利用Real time q-PCR方法,检测年龄相关性白内障患者晶状体前囊膜和正常人眼晶状体前囊膜、人晶状体上皮细胞凋亡模型和正常人晶状体上皮细胞系let-7a的表达情况;利用Lipofectamine 2000瞬时转染let-7a mimic和let-7a inhibitor,分别上调和下调人晶状体上皮细胞中let-7a的表达,并利用Real time q-PCR方法验证转染效率,利用流式细胞仪检测细胞凋亡率的变化。结果年龄相关性白内障患者晶状体前囊膜组let-7a的表达显著低于正常对照组;人晶状体上皮细胞凋亡模型组let-7a的表达显著低于正常对照组;let-7a mimic转染组let-7a的表达显著高于对照组,let-7a inhibitor转染组let-7a的表达显著低于对照组;let-7a mimic转染组细胞凋亡率显著低于对照组,let-7a inhibitor转染组细胞凋亡率显著高于对照组,差异均有统计学意义(P<0.01)。结论 micro RNA let-7a能够调控人晶状体上皮细胞凋亡,从而在白内障发病过程中发挥重要作用,micro RNA let-7a可能成为白内障非手术治疗的新靶点。 展开更多
关键词 micro RNA let-7a 细胞凋亡 白内障
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农杆菌介导的番茄Micro-Tom遗传转化体系的建立 被引量:10
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作者 陈双臣 刘爱荣 +2 位作者 王凤华 王菲 周洲 《华北农学报》 CSCD 北大核心 2010年第2期112-115,共4页
Micro-Tom番茄植株矮小,生长密度高,生命周期短,转化效率高,成为功能基因组学研究的新型模式植物。对影响Micro-Tom遗传转化频率的共培养时间、AS的添加、工程菌液浓度和抑制农杆菌所用抗生素种类进行了分析,建立了Micro-Tom稳定高效的... Micro-Tom番茄植株矮小,生长密度高,生命周期短,转化效率高,成为功能基因组学研究的新型模式植物。对影响Micro-Tom遗传转化频率的共培养时间、AS的添加、工程菌液浓度和抑制农杆菌所用抗生素种类进行了分析,建立了Micro-Tom稳定高效的遗传转化体系。以bar基因设计引物对转化群体进行PCR检测,阳性率为72.3%,平均插入位点数为1.8个。遗传体系的建立为Micro-Tom在植物生物学研究中提供理论基础。 展开更多
关键词 micro—Tom番茄 模式植物 根癌农杆菌 遗传转化
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基于STM32F103XX微处理器的Micro SD卡读写 被引量:27
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作者 徐建功 赵捷 +1 位作者 李伟 田杰 《现代电子技术》 2010年第20期26-28,32,共4页
基于STM32F103XX微控制器的远程智能心脏检测仪采用Micro SD作为存储介质保存特定的心电信号。所存Micro SD卡中的存储数据要能够在计算机上直接存取,需要存储的数据以FAT32文件格式写入Micro SD卡。研究了使用STM32F103XX微处理器,采用... 基于STM32F103XX微控制器的远程智能心脏检测仪采用Micro SD作为存储介质保存特定的心电信号。所存Micro SD卡中的存储数据要能够在计算机上直接存取,需要存储的数据以FAT32文件格式写入Micro SD卡。研究了使用STM32F103XX微处理器,采用SPI模式与Micro SD接口,将数据写入Micro SD的软件方法及硬件设计。采用的FAT开源文件系统FATFS是专门为小型嵌入式系统而设计的,容易移值和使用,占用硬件资源相对较小而且功能强大。 展开更多
关键词 STM32F103XX micro SD SPI FATFS
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应用Micro CT观察洛伐他汀和秋水仙碱对CCl_4致肝损伤后小鼠骨代谢的影响 被引量:5
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作者 吕思敏 于琼 +2 位作者 夏海珊 崔燎 吴铁 《中国药理学通报》 CAS CSCD 北大核心 2014年第8期1116-1122,共7页
目的本实验应用Micro CT研究洛伐他汀和秋水仙碱对CCl4致肝损伤后小鼠骨代谢的影响,并比较二者的效果。方法体积分数40%的CCl4花生油皮下注射,建立小鼠肝损伤模型,同时,洛伐他汀按2.6 mg·kg-1,秋水仙碱按0.065 mg·kg-1每天1... 目的本实验应用Micro CT研究洛伐他汀和秋水仙碱对CCl4致肝损伤后小鼠骨代谢的影响,并比较二者的效果。方法体积分数40%的CCl4花生油皮下注射,建立小鼠肝损伤模型,同时,洛伐他汀按2.6 mg·kg-1,秋水仙碱按0.065 mg·kg-1每天1次灌胃给药,连续1个月,于实验结束后观察肝损伤血清学指标以及肝匀浆相关的抗氧化指标,并用Micro CT测定小鼠胫骨骨组织结构参数。结果单用CCl4小鼠肝指数明显增加,AST和ALT活性明显升高,肝匀浆中SOD活性和GSH-Px水平明显降低,MDA水平明显升高,且胫骨上段骨组织结构参数BVF、TMD值明显降低,SMI值明显升高,出现明显的骨质疏松症状。而洛伐他汀、秋水仙碱用药后对肝损伤没有明显影响,但洛伐他汀对肝损伤导致的骨丢失有明显的保护作用,秋水仙碱组则没有明显的保护作用。结论 CCl4致肝损伤后的小鼠出现骨质疏松症状,洛伐他汀用药后对CCl4致肝损伤的骨丢失有明显的预防作用。 展开更多
关键词 micro CT 洛伐他汀 秋水仙碱 CCl_4 肝损伤 骨质疏松
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7.0T Micro MRI观察丝裂霉素C预防硬膜外粘连的效果研究 被引量:6
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作者 陆圣华 曹晓建 +3 位作者 王立新 孙钰 滕皋军 臧凤超 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第10期1263-1266,共4页
目的:应用7.0T Micro MRI探讨预防椎板切除后硬膜外粘连实验研究中丝裂霉素C(MMC)的理想浓度及其使用安全性。方法:30只SD大鼠随机分成5组,每组6只。分别切除L1椎板,术中各组分别以棉片浸透生理盐水(A组)或0.1mg/ml(B组)、0.3mg/ml(C组)... 目的:应用7.0T Micro MRI探讨预防椎板切除后硬膜外粘连实验研究中丝裂霉素C(MMC)的理想浓度及其使用安全性。方法:30只SD大鼠随机分成5组,每组6只。分别切除L1椎板,术中各组分别以棉片浸透生理盐水(A组)或0.1mg/ml(B组)、0.3mg/ml(C组)、0.5mg/ml(D组)、0.7mg/ml(E组)的MMC,置于裸露的硬脊膜后5min。应用MMC前后分别对大鼠行体感诱发电位测定。术后4周处死大鼠取手术段脊柱,分别做MicroMRI扫描、瘢痕组织面积测定、肉眼观察硬脊膜与后方瘢痕组织粘连情况。结果:A组标本硬膜外瘢痕组织致密,瘢痕面积大,与硬脊膜形成紧密粘连。B组和C组标本硬膜外瘢痕组织不致密,瘢痕面积减小,与硬脊膜部分粘连。D组和E组标本硬膜外瘢痕组织不致密,瘢痕面积更小,与硬脊膜未形成明显粘连。大鼠应用MMC前后体感诱发电位无明显改变。结论:局部应用浓度为0.5mg/ml的MMC能有效减少硬膜外瘢痕组织增生,避免椎板切除术后硬膜外瘢痕粘连,并且安全。0.5mg/ml浓度的MMC可能是椎板切除后预防硬膜外粘连理想的浓度选择。 展开更多
关键词 椎板切除术 硬膜 粘连 丝裂霉素C 浓度 micro MRI
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Micro CT应用于犬即刻种植即刻加载后种植体-骨界面骨结合的研究 被引量:6
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作者 张亨国 刘向辉 +3 位作者 孙卫革 张磊 沈彬 叶长林 《安徽医科大学学报》 CAS 北大核心 2014年第4期455-459,共5页
目的应用Micro CT对即刻种植即刻加载后种植体-骨界面骨结合情况进行研究。方法 4只Beagle犬,拔除其双侧下颌第3、4前磨牙,在相应的拔牙窝选择最佳位置即刻植入DIO种植体(直径3.8 mm,长度10.0 mm)各1颗,同期连接基台(直径3.8 mm,长度6.0... 目的应用Micro CT对即刻种植即刻加载后种植体-骨界面骨结合情况进行研究。方法 4只Beagle犬,拔除其双侧下颌第3、4前磨牙,在相应的拔牙窝选择最佳位置即刻植入DIO种植体(直径3.8 mm,长度10.0 mm)各1颗,同期连接基台(直径3.8 mm,长度6.0 mm)。术后24 h内采用种植体垂直加载仪进行垂直加载,将每只犬下颌的4枚种植体按照加载力的不同分为:对照组(不加载),加载5、10、15N组,加载频率为2 Hz,加载时间为每天15 min。开始加载后第4、8周后各处死2只Beagle犬,制作离体犬下颌骨标本并用Micro CT扫描,以CTAn软件分析扫描后图像,取与种植体相邻的感兴趣区行骨密度(BMD)及骨计量学分析。结果①加载4周时,加载10 N组与加载5、15 N组以及对照组在BMD、骨小梁数目、厚度、形态等方面差异均有统计学意义(P<0.05)。②加载8周时,加载10 N组与加载5、15N组以及对照组在骨小梁数目、厚度、形态等方面差异均有统计学意义(P<0.05)。③加载8周时,在骨小梁数目、厚度、形态等方面,加载15 N组与加载5、10 N组及对照组差异均有统计学意义(P<0.05)。结论应用Micro CT对种植体-骨界面新生骨骨小梁情况进行分析,客观地对不同加载时期不同加载项下种植体-骨界面骨结合情况进行了评估,为牙即刻种植术及术后即刻加载提供了理论依据。 展开更多
关键词 micro CT 即刻种植 即刻加载 种植体骨界面 骨结合
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红景天苷通过micro RNA-370改善2型糖尿病小鼠糖代谢的作用机制 被引量:9
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作者 张新茹 于玲 +1 位作者 王冬雪 谢莉娜 《医药导报》 CAS 北大核心 2018年第3期279-284,共6页
目的观察红景天苷改善2型糖尿病小鼠血糖作用,探讨红景天苷改善2型糖尿病小鼠糖代谢紊乱的分子机制。方法采用高脂饮食联合腹腔注射链脲佐菌素(STZ)建立2型糖尿病小鼠模型,检测血糖相关指标、血清和肝脏micro RNA-370表达,以及肝组织糖... 目的观察红景天苷改善2型糖尿病小鼠血糖作用,探讨红景天苷改善2型糖尿病小鼠糖代谢紊乱的分子机制。方法采用高脂饮食联合腹腔注射链脲佐菌素(STZ)建立2型糖尿病小鼠模型,检测血糖相关指标、血清和肝脏micro RNA-370表达,以及肝组织糖异生关键酶(PEPCK/G6Pase)蛋白表达水平,观察红景天苷对2型糖尿病小鼠糖代谢紊乱的改善作用。分离培养小鼠原代肝细胞,采用瞬时转染技术将micro RNA-370沉默或过表达,观察micro RNA-370对糖代谢的影响及红景天苷调节糖代谢的分子机制。结果与模型对照组比较,红景天苷各剂量组小鼠血糖相关指标均明显改善(P<0.05);血清和肝组织micro RNA-370表达水平以及肝组织PEPCK/G6Pase蛋白相对表达水平均不同程度降低,且呈剂量依赖性,中、大剂量组降低较明显(P<0.05),小剂量组有降低趋势,但差异无统计学意义。细胞实验中,与空白对照组比较,红景天苷组和micro RNA抑制剂组PEPCK/G6Pase表达均被抑制(P<0.05),micro RNA-370激动剂组PEPCK/G6Pase表达明显促进(P<0.05),红景天苷与micro RNA-370激动剂联用能逆转micro RNA-370激动剂所致PEPCK/G6Pase蛋白表达增高(P<0.05)。结论红景天苷能明显改善2型糖尿病小鼠糖代谢紊乱,且该作用至少部分通过抑制micro RNA-370实现。 展开更多
关键词 红景天苷 micro RNA-370 2型糖尿病 糖代谢紊乱
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