OBJECTIVE GubenyiliuⅡ(GYⅡ),a traditional Chinese medicine(TCM)formula used in our hospital,has shown beneficial effects in cancer patients.In this study,we investigated the molecular mechanisms underlying the benefi...OBJECTIVE GubenyiliuⅡ(GYⅡ),a traditional Chinese medicine(TCM)formula used in our hospital,has shown beneficial effects in cancer patients.In this study,we investigated the molecular mechanisms underlying the beneficial effects of GYⅡon murine breast cancer models.METHODS Inhibition of tumor growth and metastasis was evaluated by assessment of tumor weight and analysis of bioluminescent signal after a homograft inoculation.Viability of cultured breast cancer cells was determined using MTT assay andreal-time cell analysis(RTCA).Cell migratory ability was evaluated by Transwell?assay and wound healing assay.Subsequently,the potential anti-tumor and anti-metastatic mechanism was investigated by Western blotting and Immunohistochemistry.RESULTS GYⅡshowed significant inhibitory effects on tumor growth and metastasis in the murine breast cancer model.And GYⅡsuppressed theproliferation of 4T1 and MCF-7 cells in a dose-dependent manner.A better inhibitory effect on 4T1 cells proliferation and migration was found in sub-fractions(SF)of GYⅡ.Moreover,heparanase expression and degree of angiogenesis were reduced in tumor tissues.Western blotting analysis showed decreased expression of heparanase and growth factors in the cells treated with GYⅡand its sub-fractions(SF2 and SF3),there by a reduction in phosphorylation of ERK and AKT.CONCLUSION GYⅡexerts anti-tumor growth and anti-metastatic effects on murine breast cancer model.Sub-fractions 2 and 3 exhibits higher potency of the anti-tumor activity that is,at least partly,associated with decreased heparanase and growth factor sexpression,which subsequently sup-pressed activation of ERK and AKT pathways.展开更多
The p53 and CD44 expression of 10 cases in canine breast tumor were examined utilizing immunohistochemical assay with rabbit anti-mouse polyclonal antibodies against p53 or CD44, respectively. The p53 expression was s...The p53 and CD44 expression of 10 cases in canine breast tumor were examined utilizing immunohistochemical assay with rabbit anti-mouse polyclonal antibodies against p53 or CD44, respectively. The p53 expression was significantly higher in malignant than in benign breast tumor. The expression of CD44 was not significantly different in malignant breast cancer and benign breast tumor. This suggests that p53 can be used as an indicator for animal prognosis.展开更多
目的:基于临床及超声特点构建预测模型,以鉴别超声非平行位生长、乳腺影像报告和数据系统(Breast Imaging Reporting and Data System,BI⁃RADS)分为4A、4B类乳腺小肿块的良恶性,并评估其应用价值。方法:回顾性分析2020年6月—2024年5月...目的:基于临床及超声特点构建预测模型,以鉴别超声非平行位生长、乳腺影像报告和数据系统(Breast Imaging Reporting and Data System,BI⁃RADS)分为4A、4B类乳腺小肿块的良恶性,并评估其应用价值。方法:回顾性分析2020年6月—2024年5月在南京医科大学第一附属医院就诊的327例乳腺肿块患者,筛选超声诊断为BI⁃RADS 4A、4B类,非平行位生长、直径≤10 mm的肿块,按7∶3的比例随机分为训练集(n=229)和验证集(n=98)。应用Logistic回归分析鉴别乳腺良、恶性肿块的风险因素并构建诊断预测模型。以受试者工作特征(receiver operating characteristic,ROC)曲线和决策曲线分析(decision curve analysis,DCA)评估模型的鉴别效能和临床意义。结果:327例患者中恶性比例为36.1%(118/327)。经单因素和多因素Logistic回归分析,年龄、边缘、弹性和超声BI⁃RADS分类为恶性肿块的独立危险因素。纳入这4个变量建立诊断模型,并以列线图的形式展现。训练集和验证集ROC的曲线下面积分别为0.846和0.798;DCA证实应用模型预测肿块良恶性风险可以使患者获益。此外,基于列线图计算的风险评分对所有患者进行危险分层,风险评分≥0.7(后20%)的患者被列为高危组;风险评分≤0.1(前20%)的患者被列为低危组,低、高危组恶性率分别为8.8%和82.1%。结论:根据临床及超声特点构建的预测模型,可有效鉴别超声BI⁃RADS 4A、4B类非平行位乳腺小肿块的良恶性。根据列线图计算风险评分进行危险分层,低危组的恶性率仅8.8%,而高危组的恶性率为82.1%,具有较高的临床价值。展开更多
三阴性乳腺癌(triple⁃negative breast cancer,TNBC),即雌激素受体、孕激素受体和人表皮生长因子受体2均阴性表达的一种乳腺癌亚型,其在病理和分子水平上具有广泛的肿瘤异质性,且具有较强的侵袭性和转移能力。目前的治疗效果均不理想,...三阴性乳腺癌(triple⁃negative breast cancer,TNBC),即雌激素受体、孕激素受体和人表皮生长因子受体2均阴性表达的一种乳腺癌亚型,其在病理和分子水平上具有广泛的肿瘤异质性,且具有较强的侵袭性和转移能力。目前的治疗效果均不理想,治疗后复发及转移可能性大。而肿瘤声动力疗法(sonodynamic therapy,SDT)因其无创性及较高的组织穿透能力,逐步被引入各种癌症治疗的研究中。文章简要介绍了SDT的基本原理及目前基于SDT治疗TNBC的前期研究进展。展开更多
基金The project supported by National Natural Science Foundation of China(81202840,81373815)Specialized Research Fund for the Doctoral Program of Higher Education of China(20131107110014)+1 种基金Beijing Natural Science Foundation(7162084)Swedish Cancer Foundation(150815)
文摘OBJECTIVE GubenyiliuⅡ(GYⅡ),a traditional Chinese medicine(TCM)formula used in our hospital,has shown beneficial effects in cancer patients.In this study,we investigated the molecular mechanisms underlying the beneficial effects of GYⅡon murine breast cancer models.METHODS Inhibition of tumor growth and metastasis was evaluated by assessment of tumor weight and analysis of bioluminescent signal after a homograft inoculation.Viability of cultured breast cancer cells was determined using MTT assay andreal-time cell analysis(RTCA).Cell migratory ability was evaluated by Transwell?assay and wound healing assay.Subsequently,the potential anti-tumor and anti-metastatic mechanism was investigated by Western blotting and Immunohistochemistry.RESULTS GYⅡshowed significant inhibitory effects on tumor growth and metastasis in the murine breast cancer model.And GYⅡsuppressed theproliferation of 4T1 and MCF-7 cells in a dose-dependent manner.A better inhibitory effect on 4T1 cells proliferation and migration was found in sub-fractions(SF)of GYⅡ.Moreover,heparanase expression and degree of angiogenesis were reduced in tumor tissues.Western blotting analysis showed decreased expression of heparanase and growth factors in the cells treated with GYⅡand its sub-fractions(SF2 and SF3),there by a reduction in phosphorylation of ERK and AKT.CONCLUSION GYⅡexerts anti-tumor growth and anti-metastatic effects on murine breast cancer model.Sub-fractions 2 and 3 exhibits higher potency of the anti-tumor activity that is,at least partly,associated with decreased heparanase and growth factor sexpression,which subsequently sup-pressed activation of ERK and AKT pathways.
基金Natural Science Foundation of Heilongjiang Province (C2004-32)
文摘The p53 and CD44 expression of 10 cases in canine breast tumor were examined utilizing immunohistochemical assay with rabbit anti-mouse polyclonal antibodies against p53 or CD44, respectively. The p53 expression was significantly higher in malignant than in benign breast tumor. The expression of CD44 was not significantly different in malignant breast cancer and benign breast tumor. This suggests that p53 can be used as an indicator for animal prognosis.
文摘三阴性乳腺癌(triple⁃negative breast cancer,TNBC),即雌激素受体、孕激素受体和人表皮生长因子受体2均阴性表达的一种乳腺癌亚型,其在病理和分子水平上具有广泛的肿瘤异质性,且具有较强的侵袭性和转移能力。目前的治疗效果均不理想,治疗后复发及转移可能性大。而肿瘤声动力疗法(sonodynamic therapy,SDT)因其无创性及较高的组织穿透能力,逐步被引入各种癌症治疗的研究中。文章简要介绍了SDT的基本原理及目前基于SDT治疗TNBC的前期研究进展。
