The rough crystal of exopolysaccharide produced by Enterococcus durans, a strain of lactic acid bacteria screened from the intestine of a cock in our laboratory, was purified by CM-cellulose column chromatography, DEA...The rough crystal of exopolysaccharide produced by Enterococcus durans, a strain of lactic acid bacteria screened from the intestine of a cock in our laboratory, was purified by CM-cellulose column chromatography, DEAE-Sephadex A-25 ionexchange and Sephadex G-100 gel chromatography to give EPS-Ⅰ. The EPS-Ⅰ was eluted as a single peak in HPLC analysis, indicating the homogeneity of EPS-Ⅰ and free from low-molecular-weight polysaccharides. The molecular weight of the EPS-Ⅰ was determined as 42 000 by the light scattering method. The result of its elemental analysis was C 41.08% and H 7.23% without the elements of N, P and S. Monosaccharide analysis showed that it was composed of Glc and Man in a molar ratio of 4∶1. Sugar composition analysis, methylation analysis and 1H and 13C NMR spectroscopy revealed that the EPS-Ⅰ was composed of pentasaccharide repeating units. The sequence of sugar residues was determined by using two-dimensional NMR, including heteronuclear multiple-bond correlation(HMBC) and nuclear overhauser effect spectroscopy(NOESY). The structure of the pentasaccharide repeating unit of EPS-Ⅰ was given, a new excellular polysaccharide from lactic acid bacterium compared with other EPSs was reported.展开更多
对采自天津潮间带的污泥进行富集培养,从混合菌群中筛选高效产絮凝细菌。经过5次平板分离,获得一株对高岭土悬浊液具有较高絮凝活性的产絮凝细菌W17。根据形态观察和16S r RNA基因序列分析,该菌株鉴定为Enterococcus sp.W17（Genebank号...对采自天津潮间带的污泥进行富集培养,从混合菌群中筛选高效产絮凝细菌。经过5次平板分离,获得一株对高岭土悬浊液具有较高絮凝活性的产絮凝细菌W17。根据形态观察和16S r RNA基因序列分析,该菌株鉴定为Enterococcus sp.W17（Genebank号KP198609）。在不同碳源（葡萄糖、果糖、木糖、乳糖、纤维素和淀粉）、起始p H值（5.0~10.0）和盐度（4‰~70‰）等培养条件下,分别测定菌株W17生长和产絮凝性质。结果表明,菌株W17能够利用多种碳源进行生长和产絮凝,其中葡萄糖是菌株W17生长和产絮凝的最适碳源。在起始p H值5.0~10.0范围内,菌株W17产絮凝的最适起始p H值是6.0,絮凝率可达到（91±5.83）%。菌株W17在设定盐度范围内,都能够生长并具有产絮凝性质,该菌株表现出较强的耐盐性。在淡水和海水培养条件下,菌株W17的产絮凝率分别是（94±5.50）%和（87±5.67）%。产絮凝菌株W17有望应该用于废水特别是高盐废水的处理。展开更多
文摘The rough crystal of exopolysaccharide produced by Enterococcus durans, a strain of lactic acid bacteria screened from the intestine of a cock in our laboratory, was purified by CM-cellulose column chromatography, DEAE-Sephadex A-25 ionexchange and Sephadex G-100 gel chromatography to give EPS-Ⅰ. The EPS-Ⅰ was eluted as a single peak in HPLC analysis, indicating the homogeneity of EPS-Ⅰ and free from low-molecular-weight polysaccharides. The molecular weight of the EPS-Ⅰ was determined as 42 000 by the light scattering method. The result of its elemental analysis was C 41.08% and H 7.23% without the elements of N, P and S. Monosaccharide analysis showed that it was composed of Glc and Man in a molar ratio of 4∶1. Sugar composition analysis, methylation analysis and 1H and 13C NMR spectroscopy revealed that the EPS-Ⅰ was composed of pentasaccharide repeating units. The sequence of sugar residues was determined by using two-dimensional NMR, including heteronuclear multiple-bond correlation(HMBC) and nuclear overhauser effect spectroscopy(NOESY). The structure of the pentasaccharide repeating unit of EPS-Ⅰ was given, a new excellular polysaccharide from lactic acid bacterium compared with other EPSs was reported.
