The capability of recombinant human interleukin-2 ( rhIL-2) and lymphokine-activated killer (LAK) cells in the purging of normal human bone marrows contaminated with human myeloid leukemic cell lines was evaluated. Mi...The capability of recombinant human interleukin-2 ( rhIL-2) and lymphokine-activated killer (LAK) cells in the purging of normal human bone marrows contaminated with human myeloid leukemic cell lines was evaluated. Mixtures of normal human bone marrow mononuclear cells ( BMC) and K562 cells or HL-60 cells (at the BMCK562 ratio of 200:1, 100:1 or 20:1) were incubated with IL-2 with or without LAK cells at the BMC:LAK ratio of 1:1 for one or three days. The nubmers of residual K562 cells, BFU-E and CFU-GM were examined by clonogenic assays. In 200:1 mixture groups without LAK cells, the number of K562 colonies reduced by 50% with no loss of BFU-E and CFU-GM in one-day cultures, and no K562 colonies formed in three-day cultures with about 20% loss of BFU-E and CFU-GM. If the BMC.K562 ratios were 100:1 or 20:1 in the mktures, the leukemic cells could not be eliminated. When the mixtures were incubated with IL-2 and LAK cells, no leukemic cell colonies were detected in the 20:1 group following one-day展开更多
The volatile organic compounds in male and female urine were analyzed using P&T/GC-MS.More than 70 VOCs in samples were identified.Identification of compound were based on NIST05 library and relative references.Co...The volatile organic compounds in male and female urine were analyzed using P&T/GC-MS.More than 70 VOCs in samples were identified.Identification of compound were based on NIST05 library and relative references.Compounds in double urines included short-chain alcohols,ketones,aldehyde,sulfether,aliphatic hydrocarbon,aromatic hydrocarbon and chlorinated hydrocarbon.The nicotine in urine was identified.The source of VOCs in urine was briefly discussed in the article.展开更多
The pollution of VOCs was studied on both side of highway.With the determination method of purge and trap concentration coupled with gas chromatography-mass spectrometry,we found that the degree of pollution near the ...The pollution of VOCs was studied on both side of highway.With the determination method of purge and trap concentration coupled with gas chromatography-mass spectrometry,we found that the degree of pollution near the highway was not very bad.展开更多
近交衰退的清除程度是交配系统进化的主要决定因素,对作物性状的保持和作物改良也很重要。目前近交衰退清除的研究尚未在自交不亲和的植物中进行过。为此,来自加拿大麦吉尔大学的研究人员进行了研究,题为"Inbreeding depression is...近交衰退的清除程度是交配系统进化的主要决定因素,对作物性状的保持和作物改良也很重要。目前近交衰退清除的研究尚未在自交不亲和的植物中进行过。为此,来自加拿大麦吉尔大学的研究人员进行了研究,题为"Inbreeding depression is difficult to purge in self-incompatible populations ofLeavenworthia alabamica"的论文于2019年5月27日发表在《New Phytologist》上。展开更多
文摘The capability of recombinant human interleukin-2 ( rhIL-2) and lymphokine-activated killer (LAK) cells in the purging of normal human bone marrows contaminated with human myeloid leukemic cell lines was evaluated. Mixtures of normal human bone marrow mononuclear cells ( BMC) and K562 cells or HL-60 cells (at the BMCK562 ratio of 200:1, 100:1 or 20:1) were incubated with IL-2 with or without LAK cells at the BMC:LAK ratio of 1:1 for one or three days. The nubmers of residual K562 cells, BFU-E and CFU-GM were examined by clonogenic assays. In 200:1 mixture groups without LAK cells, the number of K562 colonies reduced by 50% with no loss of BFU-E and CFU-GM in one-day cultures, and no K562 colonies formed in three-day cultures with about 20% loss of BFU-E and CFU-GM. If the BMC.K562 ratios were 100:1 or 20:1 in the mktures, the leukemic cells could not be eliminated. When the mixtures were incubated with IL-2 and LAK cells, no leukemic cell colonies were detected in the 20:1 group following one-day
文摘The volatile organic compounds in male and female urine were analyzed using P&T/GC-MS.More than 70 VOCs in samples were identified.Identification of compound were based on NIST05 library and relative references.Compounds in double urines included short-chain alcohols,ketones,aldehyde,sulfether,aliphatic hydrocarbon,aromatic hydrocarbon and chlorinated hydrocarbon.The nicotine in urine was identified.The source of VOCs in urine was briefly discussed in the article.
文摘The pollution of VOCs was studied on both side of highway.With the determination method of purge and trap concentration coupled with gas chromatography-mass spectrometry,we found that the degree of pollution near the highway was not very bad.
文摘近交衰退的清除程度是交配系统进化的主要决定因素,对作物性状的保持和作物改良也很重要。目前近交衰退清除的研究尚未在自交不亲和的植物中进行过。为此,来自加拿大麦吉尔大学的研究人员进行了研究,题为"Inbreeding depression is difficult to purge in self-incompatible populations ofLeavenworthia alabamica"的论文于2019年5月27日发表在《New Phytologist》上。
