For the study of the effect of Plagodent and Palladent noble alloy dentures (OJSC "SIC 'Supermetal'", Russia), the elemental compositions of the fluids obtained from gingival sulcus of abutment teeth...For the study of the effect of Plagodent and Palladent noble alloy dentures (OJSC "SIC 'Supermetal'", Russia), the elemental compositions of the fluids obtained from gingival sulcus of abutment teeth of metal ceramic dentures with frames made of the above-stated dental alloys, have been investigated. Response of white blood cells and fibroblasts in the gingival fluid and the mixed saliva of the patients a long time after prosthetic repair, relying on the content of proinflammatory interleukins IL-1 β and IL-6, anti-inflammatory interleukins IL-4 and IL-10, the factor of tumor necrosis TNF-α and lactoferrin, has been investigated. The results obtained have convincingly proved the biosafety of the Plagodent and Palladent noble alloys.展开更多
丙型肝炎病毒（hepatitis C virus,HCV）是导致慢性肝炎的主要病原之一^（[1]）,HCV感染后20%～30%患者能自主清除,近70%可发展为慢性感染^（[2]）。免疫应答过程中分泌的各种细胞因子在病毒性肝炎转归期起重要作用[3],白细胞介素10（in...丙型肝炎病毒（hepatitis C virus,HCV）是导致慢性肝炎的主要病原之一^（[1]）,HCV感染后20%～30%患者能自主清除,近70%可发展为慢性感染^（[2]）。免疫应答过程中分泌的各种细胞因子在病毒性肝炎转归期起重要作用[3],白细胞介素10（interleukin 10,IL-10）和IL-12在宿主防御、免疫稳态中起重要调节作用。展开更多
A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cros...A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cross-priming" of T cells by host antigenpresenting cells for MHC class I restricted tumor antigensis a major pathway for induction of tumor immunity andthat is enhanced by granulocyte-macrophage colony-stimulating factor (GM-CSF), we expressed this cytokinein J558L cells. GM-CSF secreting cells were not展开更多
Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess...Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess involves T cells. We have previously reporteddecreased tumorigenicity of the murine plasmacytomaJ558L (MHC class Ⅰ^+ and class Ⅱ^-) expressing IL-2 orB7. l. When systemic immunity was analyzed,展开更多
Tumor microenvironment is composed of the tumor cells,stromal cells,microvascular tissue fluid,constitute small amount of infiltrating cells and cytokines.In recent years,more and more evidence that tumor microenviron...Tumor microenvironment is composed of the tumor cells,stromal cells,microvascular tissue fluid,constitute small amount of infiltrating cells and cytokines.In recent years,more and more evidence that tumor microenvironment play an important role in tumorigenesis.Tumor cells,immune cells and other mesenchymal cells interact and create an immunosuppressive microenvironment through a variety of immunosuppressive factors which vascular endothelial growth factor（VEGF）,transformed growth factor-β<sub>1</sub>（TGF-β<sub>1</sub>）and interleukin-10（IL-10）),which suppress immunology functions and promote tumor cells to escape immune surveillance,ultimately leading to tumor growth and metastasis.Dendritic cells（DCs）,the most potent antigen presenting cell as now known,play a key role in the anti-tumor immune process.The pre-展开更多
The prognosis for patients with primarymalignant glioma remains poor, in spite of a varietyof different forms of treatments. Broad suppressionof humoral and cell-mediated immunity is found inpatients with malignant gl...The prognosis for patients with primarymalignant glioma remains poor, in spite of a varietyof different forms of treatments. Broad suppressionof humoral and cell-mediated immunity is found inpatients with malignant gliomas. Interleukin-2 (IL-2)production and IL-2 receptor expression are展开更多
The murine interleukin-2 gene was introduced intomurine melanoma B16 cells by retroviral vector. AfterG418 selection, a clone stably secreting high level ofinterleukin-2 (1038IU per 10~6 cells per 24 hours) wasobtaine...The murine interleukin-2 gene was introduced intomurine melanoma B16 cells by retroviral vector. AfterG418 selection, a clone stably secreting high level ofinterleukin-2 (1038IU per 10~6 cells per 24 hours) wasobtained. The tumorigenicity of this clone decreasedsignificantly as compared with parental tumor cells andtumor cells transferred neomycin gene. There was notumor developed after injection of 5×10~5 IL2-transfectedB16 cells into C57BL/6 mice. However when the展开更多
With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β conv...With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β converting enzyme (ICE) gene, ahomologue of the Caenorhabiditis elegans ce1l death geneced-3, has been identified as a key inducer of apoptosis inmammalian cells. However, no study of ICE genetherapy on cancer has been reported up to now. Weconstructed recombinant retroviral vectors with展开更多
Interleukin-15 (IL-15) is a novel cytokine thatstimulates the proliferation of T cells, B cells and NKcells. Several biological activities of interleukin-15overlap those of IL-2. Here, we report that lung cancercells....Interleukin-15 (IL-15) is a novel cytokine thatstimulates the proliferation of T cells, B cells and NKcells. Several biological activities of interleukin-15overlap those of IL-2. Here, we report that lung cancercells. which were modified by IL-15 cDNA, may serve astumor vaccine that plays an antitumor role in murinemodel. IL-15 cDNA was amplified by RT-PCR withmRNA templates that were extracted from展开更多
Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to...Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to treat melanoma, renal carcinoma,prostate carcinoma, colon carcinoma,展开更多
Tumor-initiating cells(TICs)are a highly tumorigenic subpopulation of solid tumor cells that play a critical role in the initiation of cancer~[1].These tumorigenic cells resist conventional chemotherapeutic drug treat...Tumor-initiating cells(TICs)are a highly tumorigenic subpopulation of solid tumor cells that play a critical role in the initiation of cancer~[1].These tumorigenic cells resist conventional chemotherapeutic drug treatment and are assumed to be playing major roles in cancer relapses after chemotherapy~[2].However,the notion of TICs has been rather controversial.A report shows that a high percentage(】25%)of human melanoma cells can generate a tumor in a NOD-SCID interleukin-2 receptor gamma chain null mouse~[3],suggesting that there is no clonal development of solid tumors,refuting the idea of TICs.We recently developed a method of isolating TICs from cancer cell lines by culturing single individual cells of B16-F1(a melanoma cell line)into 3D soft fibrin gels~[4].In addition to being able to generate local tumors in a展开更多
It’s well known that interleukin-2 (IL-2) plays animportant role in eliciting antitumor immunityparticularly mediated by T cells. In addition, theexpression of MHC can be enhanced by IL-2 genetransfection in tumor ce...It’s well known that interleukin-2 (IL-2) plays animportant role in eliciting antitumor immunityparticularly mediated by T cells. In addition, theexpression of MHC can be enhanced by IL-2 genetransfection in tumor cells. Recent studies have indicatedthat at least two signals are required for the activation ofnaive T cells by antigen-bearing target cells: an antigen-sopific signal and a crystimulatory signal. B7-l is展开更多
文摘For the study of the effect of Plagodent and Palladent noble alloy dentures (OJSC "SIC 'Supermetal'", Russia), the elemental compositions of the fluids obtained from gingival sulcus of abutment teeth of metal ceramic dentures with frames made of the above-stated dental alloys, have been investigated. Response of white blood cells and fibroblasts in the gingival fluid and the mixed saliva of the patients a long time after prosthetic repair, relying on the content of proinflammatory interleukins IL-1 β and IL-6, anti-inflammatory interleukins IL-4 and IL-10, the factor of tumor necrosis TNF-α and lactoferrin, has been investigated. The results obtained have convincingly proved the biosafety of the Plagodent and Palladent noble alloys.
文摘丙型肝炎病毒（hepatitis C virus,HCV）是导致慢性肝炎的主要病原之一^（[1]）,HCV感染后20%～30%患者能自主清除,近70%可发展为慢性感染^（[2]）。免疫应答过程中分泌的各种细胞因子在病毒性肝炎转归期起重要作用[3],白细胞介素10（interleukin 10,IL-10）和IL-12在宿主防御、免疫稳态中起重要调节作用。
文摘A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cross-priming" of T cells by host antigenpresenting cells for MHC class I restricted tumor antigensis a major pathway for induction of tumor immunity andthat is enhanced by granulocyte-macrophage colony-stimulating factor (GM-CSF), we expressed this cytokinein J558L cells. GM-CSF secreting cells were not
文摘Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess involves T cells. We have previously reporteddecreased tumorigenicity of the murine plasmacytomaJ558L (MHC class Ⅰ^+ and class Ⅱ^-) expressing IL-2 orB7. l. When systemic immunity was analyzed,
文摘Tumor microenvironment is composed of the tumor cells,stromal cells,microvascular tissue fluid,constitute small amount of infiltrating cells and cytokines.In recent years,more and more evidence that tumor microenvironment play an important role in tumorigenesis.Tumor cells,immune cells and other mesenchymal cells interact and create an immunosuppressive microenvironment through a variety of immunosuppressive factors which vascular endothelial growth factor（VEGF）,transformed growth factor-β<sub>1</sub>（TGF-β<sub>1</sub>）and interleukin-10（IL-10）),which suppress immunology functions and promote tumor cells to escape immune surveillance,ultimately leading to tumor growth and metastasis.Dendritic cells（DCs）,the most potent antigen presenting cell as now known,play a key role in the anti-tumor immune process.The pre-
文摘The prognosis for patients with primarymalignant glioma remains poor, in spite of a varietyof different forms of treatments. Broad suppressionof humoral and cell-mediated immunity is found inpatients with malignant gliomas. Interleukin-2 (IL-2)production and IL-2 receptor expression are
文摘The murine interleukin-2 gene was introduced intomurine melanoma B16 cells by retroviral vector. AfterG418 selection, a clone stably secreting high level ofinterleukin-2 (1038IU per 10~6 cells per 24 hours) wasobtained. The tumorigenicity of this clone decreasedsignificantly as compared with parental tumor cells andtumor cells transferred neomycin gene. There was notumor developed after injection of 5×10~5 IL2-transfectedB16 cells into C57BL/6 mice. However when the
文摘With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β converting enzyme (ICE) gene, ahomologue of the Caenorhabiditis elegans ce1l death geneced-3, has been identified as a key inducer of apoptosis inmammalian cells. However, no study of ICE genetherapy on cancer has been reported up to now. Weconstructed recombinant retroviral vectors with
基金Sponsored by 863 Program for Young Scientists in China to Professor Wei He and National foundation of Natural Science to Professor Denian Ba
文摘Interleukin-15 (IL-15) is a novel cytokine thatstimulates the proliferation of T cells, B cells and NKcells. Several biological activities of interleukin-15overlap those of IL-2. Here, we report that lung cancercells. which were modified by IL-15 cDNA, may serve astumor vaccine that plays an antitumor role in murinemodel. IL-15 cDNA was amplified by RT-PCR withmRNA templates that were extracted from
文摘Interleukin-2(IL-2) has been demonstrated to beone of the most effective target genes in cancerimmunogene therapy. There are more than 20 clinicalprotocols of cancer gene therapy introducing IL-2 intotumor patients to treat melanoma, renal carcinoma,prostate carcinoma, colon carcinoma,
基金supported by the funds from Huazhong University of Science and TechnologyUS NIH grant GM072744
文摘Tumor-initiating cells(TICs)are a highly tumorigenic subpopulation of solid tumor cells that play a critical role in the initiation of cancer~[1].These tumorigenic cells resist conventional chemotherapeutic drug treatment and are assumed to be playing major roles in cancer relapses after chemotherapy~[2].However,the notion of TICs has been rather controversial.A report shows that a high percentage(】25%)of human melanoma cells can generate a tumor in a NOD-SCID interleukin-2 receptor gamma chain null mouse~[3],suggesting that there is no clonal development of solid tumors,refuting the idea of TICs.We recently developed a method of isolating TICs from cancer cell lines by culturing single individual cells of B16-F1(a melanoma cell line)into 3D soft fibrin gels~[4].In addition to being able to generate local tumors in a
文摘It’s well known that interleukin-2 (IL-2) plays animportant role in eliciting antitumor immunityparticularly mediated by T cells. In addition, theexpression of MHC can be enhanced by IL-2 genetransfection in tumor cells. Recent studies have indicatedthat at least two signals are required for the activation ofnaive T cells by antigen-bearing target cells: an antigen-sopific signal and a crystimulatory signal. B7-l is
