旨在证明miR-324-3p可以通过调控其预测的靶基因MC1R及其下游基因的表达从而对羊驼皮肤黑色素的合成产生影响。本研究在体外培养的羊驼皮肤黑色素细胞中转染miR-324-3p过表达载体,应用qRT-PCR与Western blotting分析比较各试验组中MC1R...旨在证明miR-324-3p可以通过调控其预测的靶基因MC1R及其下游基因的表达从而对羊驼皮肤黑色素的合成产生影响。本研究在体外培养的羊驼皮肤黑色素细胞中转染miR-324-3p过表达载体,应用qRT-PCR与Western blotting分析比较各试验组中MC1R基因与毛色相关基因小眼畸形相关转录因子(Microphthalmia-associtated transcription factor,Mitf)、酪氨酸酶(Tyrosinase,Tyr)、酪氨酸相关蛋白2(Tyrosinase related protein 2,Tyrp2)的表达差异性,利用酶标仪检测黑色素产量的变化。结果显示:(1)miR-324-3p在棕色与白色羊驼皮肤中均有表达,且在棕色羊驼皮肤中极显著表达(P<0.01),其相对表达量是白色的1.64倍;(2)黑色素细胞被转染了miR-324-3p过表达载体后,处理组靶基因MC1R及其下游调控基因Mitf、Tyr和Tyrp2的表达量与黑色素产量较空白对照组均有下调,且以Mitf基因表达量极显著下调(P<0.01)。综上表明,羊驼皮肤中miR-324-3p可能通过调控MC1R基因的表达,顺势下调MC1R基因下游调控基因Mitf、Tyr与Tyrp2的表达,最终对羊驼皮肤黑色素细胞中黑色素的类型及合成量产生影响。展开更多
选取苏钟种母猪16头,在第2个发情期后,按发情前期、发情期、发情后期和间情期随机分成4组。用RT-PCR检测苏钟猪发情周期不同时期preproorexin和orexin 1受体(OX1R) m RNA在下丘脑-垂体-卵巢轴中的表达。结果显示:发情周期不同时期prepro...选取苏钟种母猪16头,在第2个发情期后,按发情前期、发情期、发情后期和间情期随机分成4组。用RT-PCR检测苏钟猪发情周期不同时期preproorexin和orexin 1受体(OX1R) m RNA在下丘脑-垂体-卵巢轴中的表达。结果显示:发情周期不同时期preproorexin m RNA在下丘脑、垂体和卵巢中变化趋势一致,preproorexin m RNA在猪的发情前期表达最高,随后其表达量开始下降,在发情后期其表达最少,在间情期时又开始上升。下丘脑中OX1R m RNA在发情前期开始上升,在发情后期达到最高,随后在间情期又开始下降。垂体和卵巢中OX1 R m RNA的变化与下丘脑中OX1R m RNA的变化趋势一致。上述结果表明:orexin可能参与调控动物生殖过程。展开更多
The interactions between several peptides with low molecular weight (guest, NH2 Arg Arg Trp Trp H 2; NH2 Arg Trp Arg Trp H 3; NH2 Trp Arg Arg Trp H 4; NH2 Arg Arg Trp Trp Trp Trp H 5; NH2 Trp Trp Arg Arg Trp Trp H 6; ...The interactions between several peptides with low molecular weight (guest, NH2 Arg Arg Trp Trp H 2; NH2 Arg Trp Arg Trp H 3; NH2 Trp Arg Arg Trp H 4; NH2 Arg Arg Trp Trp Trp Trp H 5; NH2 Trp Trp Arg Arg Trp Trp H 6; NH2 Arg Arg Trp Trp Trp Trp Trp Trp H 7; NH2 Arg Arg Trp Trp Trp Trp Trp Trp Trp Trp H 8) and β cyclodextrin dimer (host, 1) bridged with the derivative of (1R, 3R) 1 aminocyclobutane cis 1,3 dicar boxylic acid were investigated by using fluorescence polarization method in buffer aqueous solution (pH 7.4) at 298K. The binding constants of the cyclodextrin dimer 1 to the guests 2 8 were determined. It was shown that there was a cooperative action of the two cavities of a cyclodextrin dimer in the binding of large substrates, and that the structure and properties of amino acid in the peptides played very important roles in the synergic complexation between host and guest.展开更多
文摘旨在证明miR-324-3p可以通过调控其预测的靶基因MC1R及其下游基因的表达从而对羊驼皮肤黑色素的合成产生影响。本研究在体外培养的羊驼皮肤黑色素细胞中转染miR-324-3p过表达载体,应用qRT-PCR与Western blotting分析比较各试验组中MC1R基因与毛色相关基因小眼畸形相关转录因子(Microphthalmia-associtated transcription factor,Mitf)、酪氨酸酶(Tyrosinase,Tyr)、酪氨酸相关蛋白2(Tyrosinase related protein 2,Tyrp2)的表达差异性,利用酶标仪检测黑色素产量的变化。结果显示:(1)miR-324-3p在棕色与白色羊驼皮肤中均有表达,且在棕色羊驼皮肤中极显著表达(P<0.01),其相对表达量是白色的1.64倍;(2)黑色素细胞被转染了miR-324-3p过表达载体后,处理组靶基因MC1R及其下游调控基因Mitf、Tyr和Tyrp2的表达量与黑色素产量较空白对照组均有下调,且以Mitf基因表达量极显著下调(P<0.01)。综上表明,羊驼皮肤中miR-324-3p可能通过调控MC1R基因的表达,顺势下调MC1R基因下游调控基因Mitf、Tyr与Tyrp2的表达,最终对羊驼皮肤黑色素细胞中黑色素的类型及合成量产生影响。
文摘选取苏钟种母猪16头,在第2个发情期后,按发情前期、发情期、发情后期和间情期随机分成4组。用RT-PCR检测苏钟猪发情周期不同时期preproorexin和orexin 1受体(OX1R) m RNA在下丘脑-垂体-卵巢轴中的表达。结果显示:发情周期不同时期preproorexin m RNA在下丘脑、垂体和卵巢中变化趋势一致,preproorexin m RNA在猪的发情前期表达最高,随后其表达量开始下降,在发情后期其表达最少,在间情期时又开始上升。下丘脑中OX1R m RNA在发情前期开始上升,在发情后期达到最高,随后在间情期又开始下降。垂体和卵巢中OX1 R m RNA的变化与下丘脑中OX1R m RNA的变化趋势一致。上述结果表明:orexin可能参与调控动物生殖过程。
文摘The interactions between several peptides with low molecular weight (guest, NH2 Arg Arg Trp Trp H 2; NH2 Arg Trp Arg Trp H 3; NH2 Trp Arg Arg Trp H 4; NH2 Arg Arg Trp Trp Trp Trp H 5; NH2 Trp Trp Arg Arg Trp Trp H 6; NH2 Arg Arg Trp Trp Trp Trp Trp Trp H 7; NH2 Arg Arg Trp Trp Trp Trp Trp Trp Trp Trp H 8) and β cyclodextrin dimer (host, 1) bridged with the derivative of (1R, 3R) 1 aminocyclobutane cis 1,3 dicar boxylic acid were investigated by using fluorescence polarization method in buffer aqueous solution (pH 7.4) at 298K. The binding constants of the cyclodextrin dimer 1 to the guests 2 8 were determined. It was shown that there was a cooperative action of the two cavities of a cyclodextrin dimer in the binding of large substrates, and that the structure and properties of amino acid in the peptides played very important roles in the synergic complexation between host and guest.
