[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of...[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of fish resources and the characteristics of their diversity is crucial for the ecological management of the Pinglu Canal.[Methods]During the spring and autumn in 2021 and 2022,a survey of fish resources and species diversity in the Pinglu Canal was conducted using multi-mesh gill nets.A total of 125 fish species were collected,belonging to 10 orders,34 families,and 89 genera.[Results]The result showed that the Pinglu Canal contained three nationally protected Class II species,two endemic species of the Qinjiang River,three anadromous/migratory species,and eight invasive species,accounting for 2.4%,1.6%,2.4%,and 6.4%of the total species,respectively.The fish community primarily consisted of mid-and bottom-dwelling,adhesive-egg-laying,and omnivorous species.The Shannon-Wiener,Simpson,Margalef,and Pielou indices of the fish community in the Pinglu Canal ranged from 2.347 to 2.757,0.081 to 0.151,3.493 to 4.382,and 0.812 to 0.892,respectively.These indices showed relatively uniform distribution across different river reaches.[Conclusion]The result indicate that the fish community structure in the Pinglu Canal is relatively uniform.The reach from the Yujiang River to the Shaping River shows higher stability,while other river reaches experience moderate or severe disturbances.This study provides supplementary baseline data on the fish community structure in the Pinglu Canal and explores the potential impact of inter-basin connectivity on fish resources,aiming to provide a scientific basis for habitat restoration assessments after the channel straightening project.展开更多
As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus s...As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography （HPLC） analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.展开更多
Microwave assisted-semi bionic extraction (MASBE) process for lignans from Fructus Forsythiae was studied. The influences of solvent pH value, microwave power, dosage of solvent and irradiation time were investigated....Microwave assisted-semi bionic extraction (MASBE) process for lignans from Fructus Forsythiae was studied. The influences of solvent pH value, microwave power, dosage of solvent and irradiation time were investigated. Optimum extracting parameters were determined by orthogonal experiments as follows: pH value of solvent at first extraction is 5.5-6.0, that at the second extraction is 7-8; microwave power is 700 W; mass ratio of Fructus Forsythiae to water is 1-12; irradiation time is 10 min, and extracting times is two. Under these optimal conditions, the yield of lignans reaches 0.364%. Compared with the conventional extraction methods, the MASBE process has the advantages of high extraction rate, high extraction selectivity.展开更多
Waste cellulosic biomass obtains various applications due to low-cost and eco-benign characteristics.A general strategy is proposed for waste cellulosic biomass to be modified with dialdehyde functional groups as inte...Waste cellulosic biomass obtains various applications due to low-cost and eco-benign characteristics.A general strategy is proposed for waste cellulosic biomass to be modified with dialdehyde functional groups as intermediates through periodate partial oxidation.Finally,aminothiourea-modified waste cellulosic biomass can be prepared through Schiff reaction.Waste corn stalk,cotton and paper as typical precursors,were used to prepare cellulosic biomass,abbreviated as AT-S,AT-C and AT-P,respectively,and their adsorption behaviors of Au(III)from the hydrochloric acid medium were investigated.The pseudo-second kinetics equation as well as the Langmuir isotherm equation can be used to depict the adsorption process,and the maximum adsorption capacities of Au(III)are21.4,19.0and3.28mol/kg for AT-S,AT-C and AT-P at298K,respectively.The adsorption capacities of Au(III)on aminothiourea modified corn stalk(AT-S)is almost357times greater than that of raw corn stalk.To the best of our knowledge,AT-S has the highest adsorption capacity towards Au(III).AT-S also displays a superior separation selectivity towards Au(III)in the presence of Cu(II),Ni(II),Co(II),Pt(VI),Pd(II)and Rh(III).Furthermore,the characterization analysis of XRD,TG,SEM,TEM and FTIR confirms that AuCl4– has been reduced to elemental Au nanoparticles and deposit onto the surface of the biomass.It shows a prospect for waste corn stalk to be used to adsorb Au(III)from liquid phase and the possible fabrication of gold nanoparticles by a general adsorption process without any reductant.展开更多
Acetobacteraceae has garnered significant attention because of its unique properties and the broad applications of the bacterial cellulose it produces.However,unlike model strains,Acetobacteraceae have few synthetic b...Acetobacteraceae has garnered significant attention because of its unique properties and the broad applications of the bacterial cellulose it produces.However,unlike model strains,Acetobacteraceae have few synthetic biology applications because they are difficult to manipulate genetically and have insufficient genetic regulatory elements,among other factors.To address this limitation,this study characterized the fundamental properties and synthetic biology elements of three commonly used bacterial cellulose-producing strains.First,the basic characteristics of the three strains,including their cellulose film production ability,division time,antibiotic susceptibility,and plasmid features,were analyzed.Two inducible promoters(pTrc and pLux101)were subsequently characterized within the three strains.The inducibility of the pTrc promoter was relatively low across the three strains(induction ratio:1.98–6.39),whereas the pLux101 promoter demonstrated a significantly greater level of inducibility within the three strains(induction ratio:87.28–216.71).Finally,through gene knockout experiments,this study identified four genes essential for bacterial cellulose film production in the genome of the Gluconacetobacter hansenii ATCC 5358 strain.This study not only enriches the library of synthetic biology elements in nonmodel strains,but also lays the foundation for the synthetic biology applications of Acetobacteraceae.展开更多
The process of bioleaching marmatite using moderately thermophilic bacteria was studied by comparing marmatite leaching performance of mesophiles and moderate thermophiles and valuating the effect of venting capacity ...The process of bioleaching marmatite using moderately thermophilic bacteria was studied by comparing marmatite leaching performance of mesophiles and moderate thermophiles and valuating the effect of venting capacity as well as pulp density on marmatite leaching performance of moderate thermophiles. The results show that moderate thermophiles have more advantages over mesophilies in bioleaching marmatite at 45 ℃ and the pulp density of 50 g/L, and the zinc extraction efficiency reaches 93.1% in 20 d. Aeration agitation can improve the transfer of O2 and CO2 in solution and promote the growth of bacteria and therefore, enhance the leaching efficiency. Under the venting levels of 50, 200 and 800 mL/min, the zinc extraction efficiencies by moderate thermophiles are 57.8%, 92.5% and 96.0%, respectively. With the increase of pulp density, the total leaching amount of valuable metals increases, however, the extraction efficiency decreases due to many reasons, such as increasing shear force leading to poorly growth condition for bacteria, etc. The zinc extraction decreases remarkably to 58.9% while the pulp density mounts up 20%.展开更多
文摘[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of fish resources and the characteristics of their diversity is crucial for the ecological management of the Pinglu Canal.[Methods]During the spring and autumn in 2021 and 2022,a survey of fish resources and species diversity in the Pinglu Canal was conducted using multi-mesh gill nets.A total of 125 fish species were collected,belonging to 10 orders,34 families,and 89 genera.[Results]The result showed that the Pinglu Canal contained three nationally protected Class II species,two endemic species of the Qinjiang River,three anadromous/migratory species,and eight invasive species,accounting for 2.4%,1.6%,2.4%,and 6.4%of the total species,respectively.The fish community primarily consisted of mid-and bottom-dwelling,adhesive-egg-laying,and omnivorous species.The Shannon-Wiener,Simpson,Margalef,and Pielou indices of the fish community in the Pinglu Canal ranged from 2.347 to 2.757,0.081 to 0.151,3.493 to 4.382,and 0.812 to 0.892,respectively.These indices showed relatively uniform distribution across different river reaches.[Conclusion]The result indicate that the fish community structure in the Pinglu Canal is relatively uniform.The reach from the Yujiang River to the Shaping River shows higher stability,while other river reaches experience moderate or severe disturbances.This study provides supplementary baseline data on the fish community structure in the Pinglu Canal and explores the potential impact of inter-basin connectivity on fish resources,aiming to provide a scientific basis for habitat restoration assessments after the channel straightening project.
基金Foundation item： Project（50925417） supported by the National Science Fund for Distinguished Young Scholars of China Project（50830301） supported by the National Natural Science Foundation of China Project（2011467062） supported by National Research Fund for Public Benefit （Environmental Protection） Industries, China
文摘As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography （HPLC） analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.
基金Project(06SK3042) supported by the Science and Technology Commission Foundation of Hunan Province, China
文摘Microwave assisted-semi bionic extraction (MASBE) process for lignans from Fructus Forsythiae was studied. The influences of solvent pH value, microwave power, dosage of solvent and irradiation time were investigated. Optimum extracting parameters were determined by orthogonal experiments as follows: pH value of solvent at first extraction is 5.5-6.0, that at the second extraction is 7-8; microwave power is 700 W; mass ratio of Fructus Forsythiae to water is 1-12; irradiation time is 10 min, and extracting times is two. Under these optimal conditions, the yield of lignans reaches 0.364%. Compared with the conventional extraction methods, the MASBE process has the advantages of high extraction rate, high extraction selectivity.
基金Projects(51504073,51404081,51672275)supported by the National Natural Science Foundation of ChinaProject(2012CBA01202)supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology,China+1 种基金Project(QianJiaoKeHe KY[2015]433)supported by the Research Program of the Education Department of Guizhou Province,ChinaProject(XJG20141104)supported by the Research Program of Talented Scholars of Guizhou Institute of Technology,China
文摘Waste cellulosic biomass obtains various applications due to low-cost and eco-benign characteristics.A general strategy is proposed for waste cellulosic biomass to be modified with dialdehyde functional groups as intermediates through periodate partial oxidation.Finally,aminothiourea-modified waste cellulosic biomass can be prepared through Schiff reaction.Waste corn stalk,cotton and paper as typical precursors,were used to prepare cellulosic biomass,abbreviated as AT-S,AT-C and AT-P,respectively,and their adsorption behaviors of Au(III)from the hydrochloric acid medium were investigated.The pseudo-second kinetics equation as well as the Langmuir isotherm equation can be used to depict the adsorption process,and the maximum adsorption capacities of Au(III)are21.4,19.0and3.28mol/kg for AT-S,AT-C and AT-P at298K,respectively.The adsorption capacities of Au(III)on aminothiourea modified corn stalk(AT-S)is almost357times greater than that of raw corn stalk.To the best of our knowledge,AT-S has the highest adsorption capacity towards Au(III).AT-S also displays a superior separation selectivity towards Au(III)in the presence of Cu(II),Ni(II),Co(II),Pt(VI),Pd(II)and Rh(III).Furthermore,the characterization analysis of XRD,TG,SEM,TEM and FTIR confirms that AuCl4– has been reduced to elemental Au nanoparticles and deposit onto the surface of the biomass.It shows a prospect for waste corn stalk to be used to adsorb Au(III)from liquid phase and the possible fabrication of gold nanoparticles by a general adsorption process without any reductant.
文摘Acetobacteraceae has garnered significant attention because of its unique properties and the broad applications of the bacterial cellulose it produces.However,unlike model strains,Acetobacteraceae have few synthetic biology applications because they are difficult to manipulate genetically and have insufficient genetic regulatory elements,among other factors.To address this limitation,this study characterized the fundamental properties and synthetic biology elements of three commonly used bacterial cellulose-producing strains.First,the basic characteristics of the three strains,including their cellulose film production ability,division time,antibiotic susceptibility,and plasmid features,were analyzed.Two inducible promoters(pTrc and pLux101)were subsequently characterized within the three strains.The inducibility of the pTrc promoter was relatively low across the three strains(induction ratio:1.98–6.39),whereas the pLux101 promoter demonstrated a significantly greater level of inducibility within the three strains(induction ratio:87.28–216.71).Finally,through gene knockout experiments,this study identified four genes essential for bacterial cellulose film production in the genome of the Gluconacetobacter hansenii ATCC 5358 strain.This study not only enriches the library of synthetic biology elements in nonmodel strains,but also lays the foundation for the synthetic biology applications of Acetobacteraceae.
基金Projects(50621063, 40646029) supported by the National Natural Science Foundation of ChinaProject(2004CB619204) supported by the Major State Basic Research Development Program of ChinaProject(NCET-06-0691) supported by the Program for New Century Excellent Talents in University
文摘The process of bioleaching marmatite using moderately thermophilic bacteria was studied by comparing marmatite leaching performance of mesophiles and moderate thermophiles and valuating the effect of venting capacity as well as pulp density on marmatite leaching performance of moderate thermophiles. The results show that moderate thermophiles have more advantages over mesophilies in bioleaching marmatite at 45 ℃ and the pulp density of 50 g/L, and the zinc extraction efficiency reaches 93.1% in 20 d. Aeration agitation can improve the transfer of O2 and CO2 in solution and promote the growth of bacteria and therefore, enhance the leaching efficiency. Under the venting levels of 50, 200 and 800 mL/min, the zinc extraction efficiencies by moderate thermophiles are 57.8%, 92.5% and 96.0%, respectively. With the increase of pulp density, the total leaching amount of valuable metals increases, however, the extraction efficiency decreases due to many reasons, such as increasing shear force leading to poorly growth condition for bacteria, etc. The zinc extraction decreases remarkably to 58.9% while the pulp density mounts up 20%.
