Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a...Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.展开更多
This study investigated the expression characteristics and clinical significance of gamma-aminobutyric acid A(GABAA)receptor subunit gamma1(GABRG1)in glioma by integrating cancer and normal tissue data from multiple d...This study investigated the expression characteristics and clinical significance of gamma-aminobutyric acid A(GABAA)receptor subunit gamma1(GABRG1)in glioma by integrating cancer and normal tissue data from multiple databases and combining them with experimental evidence.The research revealed that GABRG1 exhibited low expression levels in glioma,and its low expression was closely associated with unfavorable clinical outcomes.Furthermore,a prognostic model with strong decision-making ability was constructed using multivariate Cox regression analysis,incorporating age,isocitric dehydrogenase(IDH)mutation status,O6-methylguanine-DNA methyltransferase(MGMT)methylation status,World Health Organization(WHO)grade,and GABRG1 expression.Additionally,GABRG1 might be associated with the expression of tumor-associated macrophages and was significantly linked to immune regulatory processes.This study provided a new target for immunotherapy in glioma and offered a novel indicator for predicting and evaluating the clinical prognosis of patients.展开更多
To investigate the eco-economic thresholds of weeds and the critical period for their control,combining economic and ecological perspectives to achieve scientific weed management,four dominant weeds,Echinochloa crus-g...To investigate the eco-economic thresholds of weeds and the critical period for their control,combining economic and ecological perspectives to achieve scientific weed management,four dominant weeds,Echinochloa crus-galli(L.)P.Beauv,Chenopodium album L.,Digitaria sanguinalis(L.)Scop,and Commelina communis L.,were selected as experimental subjects,based on their common occurrence in spring maize planting areas in Northern China.A predictive model for maize yield loss caused by mixed weed populations was established.The study analyzed the eco-economic thresholds of weeds under different control measures and determined the optimal period for weed control by combining the critical control period.A logarithmic function model was developed to describe the relationship between mixed weed density and maize yield loss:y=5.9875ln(x)-6.5407(R^(2)=0.949,F=131.244,P=0.000).The optimal model for the critical period of competition between weeds and maize in maize fields was:y=-0.0027x^(2)+0.5624x-10.064(R2=0.968,F=30.513,P=0.032).When the weed density in maize fields reached 5.57 plants·m^(-2),manual weeding should be conducted promptly.When the weed density was 3.41 plants·m^(-2) or 3.48 plants·m^(-2),soil or foliar treatments should be applied,respectively.If the weed density reached 3.93 plants·m^(-2),a combination of soil and foliar treatment should be implemented.The critical period for manual weeding was 28.4 days after sowing,for soil treatment it was 19.9 days,for foliar treatment it was 21.8 days,and for the combined treatment of soil and foliar methods,it was 23.5 days after sowing.Retaining weeds for up to 15 days after maize sowing did not result in a yield loss and could even have a positive effect on maize yield.展开更多
In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E.coli)K88,microorganisms were screened from the feces of Min pigs,and their antibacterial and biological...In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E.coli)K88,microorganisms were screened from the feces of Min pigs,and their antibacterial and biological characteristics were studied.Using E.coli K88 as the indicator bacterium,the supernatant of the isolated strain from Min pigs was co-cultured with the indicator bacteria for the antibacterial test.The Bacillus velezensis(B.velezensis)strain MZ-78 showed stronger antimicrobial activity against E.coli K88 than other isolates,and its supernatant inhibited the growth of other pathogens including Salmonella typhimurium(S.typhimurium),E.coli,Staphylococcus aureus(S.aureus),and Pseudomonas aeruginosa(P.aeruginosa).Further studies showed that the selected strain MZ-78 exhibited a certain tolerance to sodium chloride conditions.The B.velezensis strain MZ-78 exhibited no hemolytic activity and was not susceptible to antibiotics.Additionally,during metabolism,it generated a certain quantity of short-chain fatty acids,including isobutyric acid and isovaleric acid.Furthermore,this strain demonstrated the capability to counteract the adhesion of E.coli K88 to porcine small intestinal epithelial cells(IPEC-J2),while also promoting their growth and proliferation.The B.velezensis strain MZ-78 isolated from Min pigs feces showed a series of probiotic characteristics and the potential to survive in the digestive tract of animals,which indicated its potential commercial application value as a probiotic alternative to antibiotics in animal husbandry.展开更多
To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province,a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the disea...To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province,a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the diseased samples collected from five Chinese herbal medicine planting areas in Heilongjiang Province between the years of 2021 and 2022.Repetitive extragenic palindromic polymerase chain reaction(Rep–PCR)was used to amplify 57 isolates of gray leaf spot pathogen on D.dasycarpus from different regions of Heilongjiang Province.The polymorphic bands amplified by three sets of primers accounted for more than 80%.Cluster analysis results showed that at a similarity coefficient of 0.67,the gray leaf spot pathogen on D.dasycarpus in Heilongjiang Province could be divided into five major genetic groups.Genetic diversity parameter analysis indicated that there were certain differences in genetic richness among the geographic populations of gray leaf spot pathogen on D.dasycarpus from different regions.Analysis of molecular variance(AMOVA)revealed that genetic variation among strains mainly originated within populations.The genetic differentiation and relationships of gray leaf spot pathogen on D.dasycarpus from different geographic regions of Heilongjiang Province indicated that genetic differentiation and kinship among populations were somewhat related to their geographic distance.The greater the geographic distance,the higher the genetic differentiation coefficient,and the lower the genetic uniformity among populations.展开更多
Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns ...Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was 25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure includedα-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca2+binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK)activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA)stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.展开更多
A machine learning-based APP may quickly and non-destructively evaluate the quality of parameters,such as hardness and anthocyanin content in blue honeysuckle berries(Lonicera caerulea L.,BHB),based on changes in peri...A machine learning-based APP may quickly and non-destructively evaluate the quality of parameters,such as hardness and anthocyanin content in blue honeysuckle berries(Lonicera caerulea L.,BHB),based on changes in pericarp color characteristics.The color feature information of the BHB pericarp was extracted,and the corresponding hardness and anthocyanin content were determined at various growing stages.Correlation analysis of BHB quality indexes was conducted by single and combined components of BHB epidermal color features.The results showed that fruit hardness had a significantly negative correlation with color feature parameter R-G,and its anthocyanin content had a significantly positive correlation with color feature parameter R.Comparing the eight models,random forest(RF)was established to evaluate the hardness and anthocyanin content of BHB according to the correlation between pericarp color features and hardness and anthocyanin content on BHB quality evaluation APP on the WeChat platform.The credibility of APP embedding RF model for evaluating hardness and anthocyanin content in BHB was validated with the determination coefficient of 0.89 and 0.93 in practice.This approach could efficiently and conveniently evaluate the quality indexes of BHB in real time and serve as a technical reference for the detection of quality indicators of other berries using smartphones.展开更多
Coronavirus is an RNA virus that can infect both humans and animals,posing a significant threat to agriculture and public health.Although coronaviruses are highly host-specific,their ability to infect multiple hosts,c...Coronavirus is an RNA virus that can infect both humans and animals,posing a significant threat to agriculture and public health.Although coronaviruses are highly host-specific,their ability to infect multiple hosts,combined with the structure of their genome,gives them a high probability of genetic recombination and mutation,leading to the creation of novel viruses.In recent years,with the establishment and development of reverse genetic manipulation techniques,substantial technical support has been provided for studying the structure and function of the coronavirus genome,the development of novel vaccines and drugs and the construction of viral expression vectors.This paper briefly described the progress in research on coronaviruses and their reverse genetic system construction strategies,aiming to provide some references for future coronavirus research.展开更多
Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a...Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.展开更多
Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a...Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.展开更多
Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a...Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.展开更多
The low-temperature spray drying technology was developed to process instant berry powder with high efficiency and higher anthocyanin retention.The maltodextrin,whey protein and inulin were selected as additives for i...The low-temperature spray drying technology was developed to process instant berry powder with high efficiency and higher anthocyanin retention.The maltodextrin,whey protein and inulin were selected as additives for instant properties formation in berry powder.The effects of inlet air temperature(40℃–80℃),vacuum degree(0.02–0.06 MPa)and additive amount on the physicochemical properties of berry powder were analyzed through solubility,anthocyanin retention and powder yield,based on moisture content and microstructure.The findings indicated that adding maltodextrin to berry enhanced the powder yield and instant solubility.Whey protein,as an additive,provided effective protection for the anthocyanins of berry powder,and the addition less than 10 g·100^(-1) g improved the powder yield.Inulin,as an additive,reduced moisture content of berry powder,which was conducive to the higher anthocyanin retention and solubility.Technique for order preference by similarity to ideal solution(TOPSIS)analysis was conducted to optimize the spray drying parameters for anthocyanin protection and solubility.The addition of 100%maltodextrin enhanced anthocyanin protection and solubility,while maintaining the desired moisture content and powder yield.This approach was used to evaluate the comprehensive quality of berry powder.This research can provide technical guidance for producing berry powder under low-temperature spray drying.展开更多
The objective of this incubation study was to comprehensively evaluate the effects of organic fertilizer and biochar,both individually and in combination,on soil respiration dynamics,key enzyme activities and the conc...The objective of this incubation study was to comprehensively evaluate the effects of organic fertilizer and biochar,both individually and in combination,on soil respiration dynamics,key enzyme activities and the concentrations of various organic carbon components in degraded mollisol.This study used a completely randomized factorial design with three application rates of organic fertilizer(M0:0,M1:13.64 g·kg^(-1),M2:27.27 g·kg^(-1))and biochar(C0:0,C1:1.36 g·kg^(-1),C2:2.73 g·kg^(-1)),resulting in nine treatments with four replicates each.Soil CO_(2) flux,organic carbon fractions including the total,active and microbial biomass carbon,and enzyme activities(sucrase,catalase and polyphenol oxidase)were measured over 120 days.Results indicated that the factors and their interactions significantly influenced all measured parameters.Soil respiration peaked at day 15,with M1C2 treatment exhibiting a rate of 10.90-fold higher than the control.The concentrations of the total,active and microbial biomass organic carbon reached maximum values on days 60,30 and 80,respectively.Notably,M2C1 treatment yielded the highest total organic carbon(86.35%increase)and microbial biomass carbon(17.84%increase).Enzyme activities were significantly enhanced,with the highest catalase activity observed for M0C2 treatment(10.14%increase).These results demonstrated that the co-application of organic fertilizer and biochar was a effective strategy for improving soil biological health and promoting carbon sequestration in degraded mollisol.The treatment combining a high rate of organic fertilizer with a low rate of biochar(M2C1)was identified as the most promising amendment strategy for fertility restoration.展开更多
In order to improve the quality of 3D printed raspberry preserves after post-processing,microwave ovens combining infrared and microwave methods were utilized.The effects of infrared heating temperature,infrared heati...In order to improve the quality of 3D printed raspberry preserves after post-processing,microwave ovens combining infrared and microwave methods were utilized.The effects of infrared heating temperature,infrared heating time,microwave power,microwave heating time on the center temperature,moisture content,the chroma(C*),the total color difference(ΔE*),shape fidelity,hardness,and the total anthocyanin content of 3D printed raspberry preserves were analyzed by response surface method(RSM).The results showed that under combining with the two methods,infrared heating improved the fidelity and quality degradation of printed products,while microwave heating enhanced the efficiency of infrared heating.Infrared-microwave combination cooking could maintain relatively stable color appearance and shape of 3D printed raspberry preserves.The AHP–CRITIC hybrid weighting method combined with the response surface test to determine the comprehensive weights of the evaluation indicators optimized the process parameters,and the optimal process parameters were obtained:infrared heating temperature of 190℃,infrared heating time of 10 min and 30 s,microwave power of 300 W,and microwave heating time of 2 min and 6 s.The 3D printed raspberry cooking methods obtained under the optimal conditions seldom had color variation,porous structure,uniform texture,and high shape fidelity,which retained the characteristics of personalized manufacturing by 3D printing.This study could provide a reference for the postprocessing and quality control of 3D cooking methods.展开更多
Esophageal cancer(EC)is one of the most common malignancies in the world,and there is no specific treatment drug for esophageal cancer yet.Doramectin(DRM)is a broad-spectrum anti-parasitic drug,and it plays an importa...Esophageal cancer(EC)is one of the most common malignancies in the world,and there is no specific treatment drug for esophageal cancer yet.Doramectin(DRM)is a broad-spectrum anti-parasitic drug,and it plays an important role in the treatment of animal diseases,while DRM has not been reported for the treatment of esophageal squamous cell carcinoma(ESCC).The purpose of this study was to investigate the anticancer effects and potential molecular mechanisms of DRM in ESCC.In the present study,the impact of DRM on the viability of ESCC was examined by methylthiazolyldiphenyl-tetrazolium bromide(MTT).Autophagy was measured by transmission electron microscopy(TEM),Western blot and immunohistochemistry.The apoptosis rate was measured by Western blot,flow cytometry and terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL).Meanwhile,autophagy inhibition was achieved by using chloroquine(CQ).After autophagy inhibition,cell proliferation and cloning ability were significantly inhibited,and the expression level of apoptotic protein was significantly changed compared with that of DRM alone.Additionally,Eca109-derived xenografts were established for testing the DRM-induced autophagy in vivo.It was found that DRM significantly inhibited the proliferation of Eca109 and EC9706 cells in vitro and in vivo in a dose-dependent manner by activating autophagy.DRM was able to significantly repress colony formation in Eca109 and EC9706 cells in vitro.At the same time,DRM could induce apoptosis of ESCC in vitro,it was also regulated through mitochondrial pathways.Meanwhile,DRM induced autophagy and inhibited the proliferation of ESCC,and exhibited little toxicity in organs in vivo.Moreover,DRM-induced autophagy could inhibit the apoptosis of EC in vitro and in vivo.Further experiment suggested that DRM might induce autophagy by the Akt/mTOR pathway.In conclusion,the present study was the first to clarify that DRM could inhibit Eca109 and EC9706 cells proliferation through activating autophagy by the Akt/mTOR pathway.DRM might be a potentially effective treatment for EC.展开更多
The purpose of this experiment was to investigate the effect and mechanism of quercetin on reproductive performance in perinatal sows.According to the similar principle of parity and body weight,24 perinatal sows were...The purpose of this experiment was to investigate the effect and mechanism of quercetin on reproductive performance in perinatal sows.According to the similar principle of parity and body weight,24 perinatal sows were randomly divided into four treatments with six replicates,including normal control and three treatments fed by a basal diet supplemented with 0,0.025%,0.050%,and 0.075%quercetin,respectively.The feeding trial was conducted from 100 days of gestation to 28 days post-partum.Reproductive performance,colostrum composition,serum indexes of antioxidation,inflammation,hormones,and the diversity and relative abundance of fecal microflora were determined in perinatal sows.Compared with the control,quercetin significantly reduced the rate of constipation,serum content of malondialdehyde(MDA),prolactin(PRL),and progesterone(PROG),and significantly increased the content of serum estradiol(E2)and insulin-like growth factor-1(IGF-1)in colostrum of perinatal sows(P<0.05).Meanwhile,0.025%quercetin significantly increased glutathione(GSH)content in serum(P<0.05),0.050%quercetin significantly reduced the rate of backfat loss(P<0.05),and 0.075%quercetin significantly increased immunoglobulin M(IgM)content in colostrum of perinatal sows(P<0.05).In addition,0.050%and 0.075%quercetin significantly shortened estrus interval(P<0.05)and significantly increased the content of IgA and IgG in colostrum(P<0.05)and dopamine(DA)content in serum of perinatal sows(P<0.05).Quercetin significantly decreased the content of nitric oxide(NO),IL-1β,IL-6,TNF-α,and MCP-1 in serum(P<0.05)and significantly improved microflora abundance and diversity in feces of perinatal sows(P<0.05).In conclusion,dietary supplementation of quercetin reduced oxidative stress and inflammatory responses and regulated intestinal microflora in perinatal sows,thus improved reproductive performance in perinatal sows.Under this experimental condition,the optimal supplementation with dietary quercetin was 0.075%in perinatal sows.展开更多
Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or C...Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or Cu polluted soils.Phytoextraction of heavy metal(HM)using tolerant plants along with organic chelators has gained global attention,and this study provided further insights into this issue.Pot experiments were performed to evaluate the effects of different types of chelators[ethylenediamine tetraacetic acid(EDTA),ethylenediamine disuccinic acid(EDDS)and citric acid(CA)]to improve the phytoextraction capacity of Ricinus communis L.for the metals Cd and Cu.Contaminated soil from a copper smelter was used in this study.A rhizon soil sampler was used to determine the metal concentrations in soil pore water.The results indicated that R.communis was an adequate candidate for chelator induced phytoextraction under the experimental conditions and that EDDS would be a good candidate chelator for the phytoextraction of Cu in soils.EDTA addition obviously improved the uptake of Cd and Cu in R.communis;however,it posed the greatest risk because the concentration of HMs in soil pore water was very high even after 40 days.Compared with EDTA and EDDS,CA had few effects on Cd or Cu uptake in R.communis.Linear relationships between the metal uptake in R.communis shoots and the maximum HM concentrations in soil pore water under HM,2.5,5,and 10 mmol·kg^(-1) treatments were typically observed.From the results of this study,it could be concluded that EDDS treatments played a promising role in increasing the uptake of Cd or Cu and reducing its phytotoxicity.EDDS application could be an effective approach for the phytoextraction of Cd or Cu from polluted soils by growing Ricinus communis L.展开更多
Soybean seed oil has always been the primary focus of researches on improving the quality of soybean.Triacylglycerol,which is composed of fatty acids and glycerol,serves as the main storage form of soybean seed oil.Th...Soybean seed oil has always been the primary focus of researches on improving the quality of soybean.Triacylglycerol,which is composed of fatty acids and glycerol,serves as the main storage form of soybean seed oil.Therefore,the biosynthesis of fatty acids and the assembly of triacylglycerol significantly affect the quality of soybean seed oil.The process of soybean seed oil synthesis was comprehensively analyzed by examining the synthesis and activation of acetyl-CoA,fatty acid biosynthesis and desaturation,triacylglycerol assembly,and other related processes,additionally,this analysis identified the key enzymes and transcription factors involved in each link of the oil synthesis process.It was found that the researches on the key enzymes of carbon source regulation,pyruvate dehydrogenase complex and acetyl-CoA carboxylase,in the genetic network of soybean seed oil synthesis,were somewhat limited.Analyzing their structures and functions would aid in further understanding the molecular mechanism of soybean fatty acid synthesis,which would be beneficial for enhancing soybean oil content and synthesis efficiency.Based on summarizing the results of genetic analysis of soybean oil and fatty acids,the prospects for mechanism analysis and advancements in biological breeding techniques for improving soybean oil quality at the molecular level were discussed.展开更多
This study aimed to investigate the effects of dietary guanidinoacetic acid on growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.Total 48042-day-old female ducks wer...This study aimed to investigate the effects of dietary guanidinoacetic acid on growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.Total 48042-day-old female ducks were randomly divided into four groups with six replicates and 20 ducks per replicate and fed the basal diet to the control group.The experimental groups were fed the basal diet with 400,600 and 800 mg·kg^(-1) guanidinoacetic acid,respectively.The trial lasted 48 days.Compared with the control group,(1)the body weight at 90 days and average daily gain were increased(P<0.05),the feed conservation ratio was decreased(P<0.05);(2)the dressing percentage and breast muscle percentage were increased(P<0.05);(3)the total amino acid content,polyunsaturated fatty acids to monounsaturated fatty acids ratio were increased(P<0.05)in breast and thigh muscles;(4)activities of superoxide dismutase,catalase and glutathione peroxidase enzyme were increased(P<0.05)in thigh muscles;(5)the relative expressions of superoxide dismutase 1,glutathione peroxidase 1,and catalase were significantly increased(P<0.05)in the liver.In this study,the optimum dosage of 600 mg·kg^(-1) guanidinoacetic acid improved the growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.展开更多
The trace element selenium(Se)occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se ...The trace element selenium(Se)occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se deficiency accusers more frequently in low-Se regions.Therefore,to investigate the Se status and inflammatory response of the mammary gland under marginal dietary Se levels,an lipopolysaccharide(LPS)induced mouse mastitis model was established.Mice were fed with moderate Se diet(0.087 mg•kg^(-1) Se),adequate Se diet(0.15 mg•kg^(-1) Se)or excessive Se diet(1.5 mg•kg^(-1) Se)for 60 days.Se status and inflammatory factors were investigated.Results showed that the Se status of mammary gland correlated with dietary Se levels.Marginal Se deficiency exacerbated mammary tissue histopathology;increased the mRNA level of inflammatory genes tumor necrosis factor alpha(TNF-α),interleukin-1β(IL-1β)and cyclooxygenase-2(COX-2);and enhanced the phosphorylation of NF-κB p65 in mammary gland tissues.Supplementation of Se in diet higher than recommended levels reduced the inflammatory reaction of mammary glands in LPS-induced mastitis model and provided a protective effect.展开更多
文摘Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.
基金Supported by the National Natural Science Foundation of China(82271340,82071368)the Outstanding Youth Funding of Harbin Medical University(HYD2020JQ0016)the Horizontal Subject(86010900)。
文摘This study investigated the expression characteristics and clinical significance of gamma-aminobutyric acid A(GABAA)receptor subunit gamma1(GABRG1)in glioma by integrating cancer and normal tissue data from multiple databases and combining them with experimental evidence.The research revealed that GABRG1 exhibited low expression levels in glioma,and its low expression was closely associated with unfavorable clinical outcomes.Furthermore,a prognostic model with strong decision-making ability was constructed using multivariate Cox regression analysis,incorporating age,isocitric dehydrogenase(IDH)mutation status,O6-methylguanine-DNA methyltransferase(MGMT)methylation status,World Health Organization(WHO)grade,and GABRG1 expression.Additionally,GABRG1 might be associated with the expression of tumor-associated macrophages and was significantly linked to immune regulatory processes.This study provided a new target for immunotherapy in glioma and offered a novel indicator for predicting and evaluating the clinical prognosis of patients.
基金Supported by the National Key Research and Development Program of China(2023YFD1400502)。
文摘To investigate the eco-economic thresholds of weeds and the critical period for their control,combining economic and ecological perspectives to achieve scientific weed management,four dominant weeds,Echinochloa crus-galli(L.)P.Beauv,Chenopodium album L.,Digitaria sanguinalis(L.)Scop,and Commelina communis L.,were selected as experimental subjects,based on their common occurrence in spring maize planting areas in Northern China.A predictive model for maize yield loss caused by mixed weed populations was established.The study analyzed the eco-economic thresholds of weeds under different control measures and determined the optimal period for weed control by combining the critical control period.A logarithmic function model was developed to describe the relationship between mixed weed density and maize yield loss:y=5.9875ln(x)-6.5407(R^(2)=0.949,F=131.244,P=0.000).The optimal model for the critical period of competition between weeds and maize in maize fields was:y=-0.0027x^(2)+0.5624x-10.064(R2=0.968,F=30.513,P=0.032).When the weed density in maize fields reached 5.57 plants·m^(-2),manual weeding should be conducted promptly.When the weed density was 3.41 plants·m^(-2) or 3.48 plants·m^(-2),soil or foliar treatments should be applied,respectively.If the weed density reached 3.93 plants·m^(-2),a combination of soil and foliar treatment should be implemented.The critical period for manual weeding was 28.4 days after sowing,for soil treatment it was 19.9 days,for foliar treatment it was 21.8 days,and for the combined treatment of soil and foliar methods,it was 23.5 days after sowing.Retaining weeds for up to 15 days after maize sowing did not result in a yield loss and could even have a positive effect on maize yield.
基金Supported by the Natural Science Foundation of Heilongjiang Province of China(LH2021C038)the Earmarked Fund for CARS-35the National Key R&D Program of China(2018YFD0501202)。
文摘In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E.coli)K88,microorganisms were screened from the feces of Min pigs,and their antibacterial and biological characteristics were studied.Using E.coli K88 as the indicator bacterium,the supernatant of the isolated strain from Min pigs was co-cultured with the indicator bacteria for the antibacterial test.The Bacillus velezensis(B.velezensis)strain MZ-78 showed stronger antimicrobial activity against E.coli K88 than other isolates,and its supernatant inhibited the growth of other pathogens including Salmonella typhimurium(S.typhimurium),E.coli,Staphylococcus aureus(S.aureus),and Pseudomonas aeruginosa(P.aeruginosa).Further studies showed that the selected strain MZ-78 exhibited a certain tolerance to sodium chloride conditions.The B.velezensis strain MZ-78 exhibited no hemolytic activity and was not susceptible to antibiotics.Additionally,during metabolism,it generated a certain quantity of short-chain fatty acids,including isobutyric acid and isovaleric acid.Furthermore,this strain demonstrated the capability to counteract the adhesion of E.coli K88 to porcine small intestinal epithelial cells(IPEC-J2),while also promoting their growth and proliferation.The B.velezensis strain MZ-78 isolated from Min pigs feces showed a series of probiotic characteristics and the potential to survive in the digestive tract of animals,which indicated its potential commercial application value as a probiotic alternative to antibiotics in animal husbandry.
基金Supported by the Green Plant Protection Project of Heilongjiang Province(2130108)Key R&D Program Project of Heilongjiang Province(2023ZX02B0502)Heilongjiang Province Rice Modern Agriculture Industry Technology Collaborative Innovation System Project(2025)。
文摘To ascertain the genetic diversity of gray leaf spot pathogen on Dictamnus dasycarpus popoulation in Heilongjiang Province,a total of 57 strains of Paracercospora dictamnicola were isolated and purified from the diseased samples collected from five Chinese herbal medicine planting areas in Heilongjiang Province between the years of 2021 and 2022.Repetitive extragenic palindromic polymerase chain reaction(Rep–PCR)was used to amplify 57 isolates of gray leaf spot pathogen on D.dasycarpus from different regions of Heilongjiang Province.The polymorphic bands amplified by three sets of primers accounted for more than 80%.Cluster analysis results showed that at a similarity coefficient of 0.67,the gray leaf spot pathogen on D.dasycarpus in Heilongjiang Province could be divided into five major genetic groups.Genetic diversity parameter analysis indicated that there were certain differences in genetic richness among the geographic populations of gray leaf spot pathogen on D.dasycarpus from different regions.Analysis of molecular variance(AMOVA)revealed that genetic variation among strains mainly originated within populations.The genetic differentiation and relationships of gray leaf spot pathogen on D.dasycarpus from different geographic regions of Heilongjiang Province indicated that genetic differentiation and kinship among populations were somewhat related to their geographic distance.The greater the geographic distance,the higher the genetic differentiation coefficient,and the lower the genetic uniformity among populations.
基金Supported by the National Key R&D Program(2022YFF1300500)the Spring Goose Support Program(CYQN24018)the National Natural Science Foundation of China(32572123)。
文摘Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was 25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure includedα-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca2+binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK)activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA)stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.
基金Supported by the National Natural Science Foundation of China(32072352)the National Key Research and Development Program Project of China(2022YFD1600500)。
文摘A machine learning-based APP may quickly and non-destructively evaluate the quality of parameters,such as hardness and anthocyanin content in blue honeysuckle berries(Lonicera caerulea L.,BHB),based on changes in pericarp color characteristics.The color feature information of the BHB pericarp was extracted,and the corresponding hardness and anthocyanin content were determined at various growing stages.Correlation analysis of BHB quality indexes was conducted by single and combined components of BHB epidermal color features.The results showed that fruit hardness had a significantly negative correlation with color feature parameter R-G,and its anthocyanin content had a significantly positive correlation with color feature parameter R.Comparing the eight models,random forest(RF)was established to evaluate the hardness and anthocyanin content of BHB according to the correlation between pericarp color features and hardness and anthocyanin content on BHB quality evaluation APP on the WeChat platform.The credibility of APP embedding RF model for evaluating hardness and anthocyanin content in BHB was validated with the determination coefficient of 0.89 and 0.93 in practice.This approach could efficiently and conveniently evaluate the quality indexes of BHB in real time and serve as a technical reference for the detection of quality indicators of other berries using smartphones.
基金Supported by the National Natural Science Foundation of China Joint Foundation Programme(U22A20527)。
文摘Coronavirus is an RNA virus that can infect both humans and animals,posing a significant threat to agriculture and public health.Although coronaviruses are highly host-specific,their ability to infect multiple hosts,combined with the structure of their genome,gives them a high probability of genetic recombination and mutation,leading to the creation of novel viruses.In recent years,with the establishment and development of reverse genetic manipulation techniques,substantial technical support has been provided for studying the structure and function of the coronavirus genome,the development of novel vaccines and drugs and the construction of viral expression vectors.This paper briefly described the progress in research on coronaviruses and their reverse genetic system construction strategies,aiming to provide some references for future coronavirus research.
文摘Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.
文摘Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.
文摘Aims and Scope Journal of Northeast Agricultural University(English Edition)is a comprehensive academic journal on agricultural sciences sponsored by Northeast Agricultural University and distributed worldwide.It is a peer reviewed journal published quarterly and mainly publishes review and research articles that reflect the latest achievements on crop science,horticulture,plant protection,resource and environment,animal science,veterinary medicine,agricultural engineering and technology,agricultural water conservancy,life science,biotechnology and food science.
基金Supported by the National Natural Science Foundation of China(32072352)。
文摘The low-temperature spray drying technology was developed to process instant berry powder with high efficiency and higher anthocyanin retention.The maltodextrin,whey protein and inulin were selected as additives for instant properties formation in berry powder.The effects of inlet air temperature(40℃–80℃),vacuum degree(0.02–0.06 MPa)and additive amount on the physicochemical properties of berry powder were analyzed through solubility,anthocyanin retention and powder yield,based on moisture content and microstructure.The findings indicated that adding maltodextrin to berry enhanced the powder yield and instant solubility.Whey protein,as an additive,provided effective protection for the anthocyanins of berry powder,and the addition less than 10 g·100^(-1) g improved the powder yield.Inulin,as an additive,reduced moisture content of berry powder,which was conducive to the higher anthocyanin retention and solubility.Technique for order preference by similarity to ideal solution(TOPSIS)analysis was conducted to optimize the spray drying parameters for anthocyanin protection and solubility.The addition of 100%maltodextrin enhanced anthocyanin protection and solubility,while maintaining the desired moisture content and powder yield.This approach was used to evaluate the comprehensive quality of berry powder.This research can provide technical guidance for producing berry powder under low-temperature spray drying.
基金Supported by the Special Fund for Agro-scientific Research in Public Interest in China(201503119-06-01)。
文摘The objective of this incubation study was to comprehensively evaluate the effects of organic fertilizer and biochar,both individually and in combination,on soil respiration dynamics,key enzyme activities and the concentrations of various organic carbon components in degraded mollisol.This study used a completely randomized factorial design with three application rates of organic fertilizer(M0:0,M1:13.64 g·kg^(-1),M2:27.27 g·kg^(-1))and biochar(C0:0,C1:1.36 g·kg^(-1),C2:2.73 g·kg^(-1)),resulting in nine treatments with four replicates each.Soil CO_(2) flux,organic carbon fractions including the total,active and microbial biomass carbon,and enzyme activities(sucrase,catalase and polyphenol oxidase)were measured over 120 days.Results indicated that the factors and their interactions significantly influenced all measured parameters.Soil respiration peaked at day 15,with M1C2 treatment exhibiting a rate of 10.90-fold higher than the control.The concentrations of the total,active and microbial biomass organic carbon reached maximum values on days 60,30 and 80,respectively.Notably,M2C1 treatment yielded the highest total organic carbon(86.35%increase)and microbial biomass carbon(17.84%increase).Enzyme activities were significantly enhanced,with the highest catalase activity observed for M0C2 treatment(10.14%increase).These results demonstrated that the co-application of organic fertilizer and biochar was a effective strategy for improving soil biological health and promoting carbon sequestration in degraded mollisol.The treatment combining a high rate of organic fertilizer with a low rate of biochar(M2C1)was identified as the most promising amendment strategy for fertility restoration.
基金Supported by the National Natural Science Foundation of China(32072352)。
文摘In order to improve the quality of 3D printed raspberry preserves after post-processing,microwave ovens combining infrared and microwave methods were utilized.The effects of infrared heating temperature,infrared heating time,microwave power,microwave heating time on the center temperature,moisture content,the chroma(C*),the total color difference(ΔE*),shape fidelity,hardness,and the total anthocyanin content of 3D printed raspberry preserves were analyzed by response surface method(RSM).The results showed that under combining with the two methods,infrared heating improved the fidelity and quality degradation of printed products,while microwave heating enhanced the efficiency of infrared heating.Infrared-microwave combination cooking could maintain relatively stable color appearance and shape of 3D printed raspberry preserves.The AHP–CRITIC hybrid weighting method combined with the response surface test to determine the comprehensive weights of the evaluation indicators optimized the process parameters,and the optimal process parameters were obtained:infrared heating temperature of 190℃,infrared heating time of 10 min and 30 s,microwave power of 300 W,and microwave heating time of 2 min and 6 s.The 3D printed raspberry cooking methods obtained under the optimal conditions seldom had color variation,porous structure,uniform texture,and high shape fidelity,which retained the characteristics of personalized manufacturing by 3D printing.This study could provide a reference for the postprocessing and quality control of 3D cooking methods.
基金Supported by the Academic Backbone Fund of Northeast Agricultural University(19XG20)the Excellent Young Scholars Fund of Harbin Medical University(HYD2020JQ0016)。
文摘Esophageal cancer(EC)is one of the most common malignancies in the world,and there is no specific treatment drug for esophageal cancer yet.Doramectin(DRM)is a broad-spectrum anti-parasitic drug,and it plays an important role in the treatment of animal diseases,while DRM has not been reported for the treatment of esophageal squamous cell carcinoma(ESCC).The purpose of this study was to investigate the anticancer effects and potential molecular mechanisms of DRM in ESCC.In the present study,the impact of DRM on the viability of ESCC was examined by methylthiazolyldiphenyl-tetrazolium bromide(MTT).Autophagy was measured by transmission electron microscopy(TEM),Western blot and immunohistochemistry.The apoptosis rate was measured by Western blot,flow cytometry and terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL).Meanwhile,autophagy inhibition was achieved by using chloroquine(CQ).After autophagy inhibition,cell proliferation and cloning ability were significantly inhibited,and the expression level of apoptotic protein was significantly changed compared with that of DRM alone.Additionally,Eca109-derived xenografts were established for testing the DRM-induced autophagy in vivo.It was found that DRM significantly inhibited the proliferation of Eca109 and EC9706 cells in vitro and in vivo in a dose-dependent manner by activating autophagy.DRM was able to significantly repress colony formation in Eca109 and EC9706 cells in vitro.At the same time,DRM could induce apoptosis of ESCC in vitro,it was also regulated through mitochondrial pathways.Meanwhile,DRM induced autophagy and inhibited the proliferation of ESCC,and exhibited little toxicity in organs in vivo.Moreover,DRM-induced autophagy could inhibit the apoptosis of EC in vitro and in vivo.Further experiment suggested that DRM might induce autophagy by the Akt/mTOR pathway.In conclusion,the present study was the first to clarify that DRM could inhibit Eca109 and EC9706 cells proliferation through activating autophagy by the Akt/mTOR pathway.DRM might be a potentially effective treatment for EC.
基金Supported by the National Natural Science Foundation of China(32072749)。
文摘The purpose of this experiment was to investigate the effect and mechanism of quercetin on reproductive performance in perinatal sows.According to the similar principle of parity and body weight,24 perinatal sows were randomly divided into four treatments with six replicates,including normal control and three treatments fed by a basal diet supplemented with 0,0.025%,0.050%,and 0.075%quercetin,respectively.The feeding trial was conducted from 100 days of gestation to 28 days post-partum.Reproductive performance,colostrum composition,serum indexes of antioxidation,inflammation,hormones,and the diversity and relative abundance of fecal microflora were determined in perinatal sows.Compared with the control,quercetin significantly reduced the rate of constipation,serum content of malondialdehyde(MDA),prolactin(PRL),and progesterone(PROG),and significantly increased the content of serum estradiol(E2)and insulin-like growth factor-1(IGF-1)in colostrum of perinatal sows(P<0.05).Meanwhile,0.025%quercetin significantly increased glutathione(GSH)content in serum(P<0.05),0.050%quercetin significantly reduced the rate of backfat loss(P<0.05),and 0.075%quercetin significantly increased immunoglobulin M(IgM)content in colostrum of perinatal sows(P<0.05).In addition,0.050%and 0.075%quercetin significantly shortened estrus interval(P<0.05)and significantly increased the content of IgA and IgG in colostrum(P<0.05)and dopamine(DA)content in serum of perinatal sows(P<0.05).Quercetin significantly decreased the content of nitric oxide(NO),IL-1β,IL-6,TNF-α,and MCP-1 in serum(P<0.05)and significantly improved microflora abundance and diversity in feces of perinatal sows(P<0.05).In conclusion,dietary supplementation of quercetin reduced oxidative stress and inflammatory responses and regulated intestinal microflora in perinatal sows,thus improved reproductive performance in perinatal sows.Under this experimental condition,the optimal supplementation with dietary quercetin was 0.075%in perinatal sows.
基金Supported by the Joint Funds of the National Natural Science Foundation of China(U2340219)。
文摘Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or Cu polluted soils.Phytoextraction of heavy metal(HM)using tolerant plants along with organic chelators has gained global attention,and this study provided further insights into this issue.Pot experiments were performed to evaluate the effects of different types of chelators[ethylenediamine tetraacetic acid(EDTA),ethylenediamine disuccinic acid(EDDS)and citric acid(CA)]to improve the phytoextraction capacity of Ricinus communis L.for the metals Cd and Cu.Contaminated soil from a copper smelter was used in this study.A rhizon soil sampler was used to determine the metal concentrations in soil pore water.The results indicated that R.communis was an adequate candidate for chelator induced phytoextraction under the experimental conditions and that EDDS would be a good candidate chelator for the phytoextraction of Cu in soils.EDTA addition obviously improved the uptake of Cd and Cu in R.communis;however,it posed the greatest risk because the concentration of HMs in soil pore water was very high even after 40 days.Compared with EDTA and EDDS,CA had few effects on Cd or Cu uptake in R.communis.Linear relationships between the metal uptake in R.communis shoots and the maximum HM concentrations in soil pore water under HM,2.5,5,and 10 mmol·kg^(-1) treatments were typically observed.From the results of this study,it could be concluded that EDDS treatments played a promising role in increasing the uptake of Cd or Cu and reducing its phytotoxicity.EDDS application could be an effective approach for the phytoextraction of Cd or Cu from polluted soils by growing Ricinus communis L.
基金Supported by the Heilongjiang Natural Science Foundation Joint Guidance Project(LH2021C028)。
文摘Soybean seed oil has always been the primary focus of researches on improving the quality of soybean.Triacylglycerol,which is composed of fatty acids and glycerol,serves as the main storage form of soybean seed oil.Therefore,the biosynthesis of fatty acids and the assembly of triacylglycerol significantly affect the quality of soybean seed oil.The process of soybean seed oil synthesis was comprehensively analyzed by examining the synthesis and activation of acetyl-CoA,fatty acid biosynthesis and desaturation,triacylglycerol assembly,and other related processes,additionally,this analysis identified the key enzymes and transcription factors involved in each link of the oil synthesis process.It was found that the researches on the key enzymes of carbon source regulation,pyruvate dehydrogenase complex and acetyl-CoA carboxylase,in the genetic network of soybean seed oil synthesis,were somewhat limited.Analyzing their structures and functions would aid in further understanding the molecular mechanism of soybean fatty acid synthesis,which would be beneficial for enhancing soybean oil content and synthesis efficiency.Based on summarizing the results of genetic analysis of soybean oil and fatty acids,the prospects for mechanism analysis and advancements in biological breeding techniques for improving soybean oil quality at the molecular level were discussed.
基金Supported by Hainan Provincial Natural Science Foundation of China(322MS134)。
文摘This study aimed to investigate the effects of dietary guanidinoacetic acid on growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.Total 48042-day-old female ducks were randomly divided into four groups with six replicates and 20 ducks per replicate and fed the basal diet to the control group.The experimental groups were fed the basal diet with 400,600 and 800 mg·kg^(-1) guanidinoacetic acid,respectively.The trial lasted 48 days.Compared with the control group,(1)the body weight at 90 days and average daily gain were increased(P<0.05),the feed conservation ratio was decreased(P<0.05);(2)the dressing percentage and breast muscle percentage were increased(P<0.05);(3)the total amino acid content,polyunsaturated fatty acids to monounsaturated fatty acids ratio were increased(P<0.05)in breast and thigh muscles;(4)activities of superoxide dismutase,catalase and glutathione peroxidase enzyme were increased(P<0.05)in thigh muscles;(5)the relative expressions of superoxide dismutase 1,glutathione peroxidase 1,and catalase were significantly increased(P<0.05)in the liver.In this study,the optimum dosage of 600 mg·kg^(-1) guanidinoacetic acid improved the growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.
基金Supported by the Natural Science Foundation of the Heilongjiang Province of China(C2016003)China Postdoctoral Science Foundation(2015M581415)Heilongjiang Postdoctoral Fund(LBH-Z15005)。
文摘The trace element selenium(Se)occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se deficiency accusers more frequently in low-Se regions.Therefore,to investigate the Se status and inflammatory response of the mammary gland under marginal dietary Se levels,an lipopolysaccharide(LPS)induced mouse mastitis model was established.Mice were fed with moderate Se diet(0.087 mg•kg^(-1) Se),adequate Se diet(0.15 mg•kg^(-1) Se)or excessive Se diet(1.5 mg•kg^(-1) Se)for 60 days.Se status and inflammatory factors were investigated.Results showed that the Se status of mammary gland correlated with dietary Se levels.Marginal Se deficiency exacerbated mammary tissue histopathology;increased the mRNA level of inflammatory genes tumor necrosis factor alpha(TNF-α),interleukin-1β(IL-1β)and cyclooxygenase-2(COX-2);and enhanced the phosphorylation of NF-κB p65 in mammary gland tissues.Supplementation of Se in diet higher than recommended levels reduced the inflammatory reaction of mammary glands in LPS-induced mastitis model and provided a protective effect.
