The establishment of high frequency regeneration system is a foundation for Agrobacterium mediated genetic transformation. In this work, several important factors influencing the efficiency of regeneration of pla...The establishment of high frequency regeneration system is a foundation for Agrobacterium mediated genetic transformation. In this work, several important factors influencing the efficiency of regeneration of plants, such as concentration of plant growth regulators, leaf explant orientation, leaf growth sequence and leaf segment, were studied. The results indicated that the differentiation rate of adventitious shoots was 90% on basal MS medium only supplemented with 1 5?mg·L -1 BA (6 benzyladenine) and reached the highest level(95%) when 1 0?mg·L -1 BA and 0 3?mg·L -1 NAA (naphthaleneacetic acid) were added to MS medium. 90% of differentiation rate of adventitious roots were obtained when 0 3?mg·L -1 NAA was only added to MS medium. It was also found that more adventitious shoots were regenerated from the lower segment of leaf (with petiole) than the other segments, the number of adventitious shoots decreased from top to base of leaf growth sequence and the percentage of adventitious shoot induction with adaxial side downward was higher than that with adaxial side upward.展开更多
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “o...The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.展开更多
Expression of rol genes from Ri plasmid of Agrobacterium rhizogenes not only leads to the excessive formation of adventitious roots, but also exhibits various genetically modified characteristics that have bro...Expression of rol genes from Ri plasmid of Agrobacterium rhizogenes not only leads to the excessive formation of adventitious roots, but also exhibits various genetically modified characteristics that have broad prospects for the application of plant genetic improvement. Since the 1980s of the last century, much progress has been made in the studies of A. rhizogenes, in particular the agropine type Ri plasmid rol genes and their applications for plant genetic improvement, which involves the structure and function of Ri plasmid, the characters of rol genes, the influence of rol genes expression on plants growth and development, and the applications of rol genes for genetic improvement of forest tree. In this paper, the advances in this field are reviewed and the existing problems about the application of rol genes for genetic improvement of forest tree are also discussed.展开更多
Selection of fire resistant tree species for the southwestern China and the planting of those species can effectively prevent large area's fire damage. In this paper the components and flammability of leaves, twi...Selection of fire resistant tree species for the southwestern China and the planting of those species can effectively prevent large area's fire damage. In this paper the components and flammability of leaves, twigs and barks of 12 tree species in the mountain area of southwestern China have been tested and analyzed in the laboratory. The test and analysis indicate the results as follows:(1) for all the tree species, the fire resistance of leaves is much weaker than that of twigs and barks, and the broad leaves are stronger than those of conifers in fire resistance. (2) Heat value, moisture, ignition point and ash content are main indexes to affect fire resistance. Heat value relates to lignose content and benzene ethanol extractive content linearly.(3) Of all the 12 tree species, Schima superba,Castanopsis hystrix, Myrica rubra have the strongest resistance to fire; Machilus pauhoi, Michelia macclurei, Mytilaria laosensis, Camellia olifera and Manglietia tenuipes are relatively strong in fire resistance, and Lithocapus thalassica, Tsoongiodendron odorum, Cunninghamia lanceolata and Pinus massoniana are weak in fire resistance.展开更多
On the basis of natural conditions of lack of rainfall on the Loess Plateau, this paper presents a method of design and construction in rainwater harvesting system for afforestation, which is suitable to the regions o...On the basis of natural conditions of lack of rainfall on the Loess Plateau, this paper presents a method of design and construction in rainwater harvesting system for afforestation, which is suitable to the regions of 300 600?mm rainfall. The system consists of micro catchments of various forms and sizes that include planting areas, where trees are planted and trapped runoff, and contributing areas, where overland flow are produced by rainfall. The design is based on rainfall, runoff coefficients of contributing areas, evapotranspiration of trees and soil surface, water deficiency of soil, and environmental capacity of precipitation in the region, and so on. Runoff coefficient of contributing areas with YJG (organic silicon chemical treatment), compacted surface soil slope and natural slope micro catchments, are 0 8 0 89, 0 23 0 36, and 0 08 0 10 respectively. According to the Penman method, the soil water deficiency varies from 50?mm to 300?mm. In the region of 400?mm precipitation, the contributing area treated with YJG is 3 4?m 2 for timber forests, 8 10?m 2 for cash trees respectively; the contributing area treated with compacted soil surface is 6 8?m\+2 for timber forests, 10 12?m 2 for cash trees respectively; the contributing area of natural slope is 8 10?m 2 for timber forests, 12 15?m 2 for cash trees respectively. Transpiration from trees of micro catchment in YJG, compacted surface soil and natural slope treatment is by 47 65% 53 31%, 24 10% 36 93%, and 18 65% 29 55% of total rainfall (rainfall and harvested rainwater) respectively after the system was applied in the region. This system, which has been widely practising on the Loess Plateau, is now known as runoff forestry.展开更多
The property of major cellulases from the guts of Anoplophora glabripennis larvae have been characterized. The optimal temperatures of both β 1,4 glucosidase (β glucosidase) and endo β 1,4 endoglucanase (...The property of major cellulases from the guts of Anoplophora glabripennis larvae have been characterized. The optimal temperatures of both β 1,4 glucosidase (β glucosidase) and endo β 1,4 endoglucanase (endoglucanase, Cx) are 40℃. The β glucosidase was optimally active at pH 4\^8, while the optimal activity of the endoglucanase occurred at pH 4 4 5 6 The endoglucanase was active with a wide range of pH and temperature, the levels of activity from 25℃ to 50℃ were more than 80%, and the activity remained 60% between pH 3 2 and pH 7 2. The endoglucanase exhibited higher thermal stability than β glucosidase. Both enzymes lose their activities by heat treatment at 60℃. Two isozymes of endoglucanase were detected in sodium carboxymethylcellulose polyacrymide gels (CMC gel) by chemical colorization, and purified by elution from the gel slices. The molecular weights of the two isozymes were estimated as 26kD and 39kD respectively. Moreover molecular characteristics of the two isozymes are currently underway.展开更多
It is well known that, in most cases, soil water doesn't move in the form of laminar flow as described by Darcy law. Only when Reynolds number ( Re ) is no more than 10, does water movement follow Darcy law. A s...It is well known that, in most cases, soil water doesn't move in the form of laminar flow as described by Darcy law. Only when Reynolds number ( Re ) is no more than 10, does water movement follow Darcy law. A soil profile with 2 9 m long and 2 13 2 60 m deep was excavated on a lower slope located at Zigui County, Hubei Province, China. Field observation found that soil pipes were mainly distributed in the transient layer between horizon B with higher degree of granite weathering and horizon C with lower degree of granite weathering. At the foot of the slope, about 5 7 soil pipes per meter were observed along the vertical direction of the slope. The observed results, obtained by continuous observation of soil pipes and pipe flow processes at granite slope for many rainfall events, indicate that the relationship between velocity of pipe flow and hydraulic gradient along the pipe is parabolic rather than linear. Based on the investigated data of soil, landform, and land use etc., combined with observed data of pipe flow derived from many rainfall events, a pipe flow model was developed. For velocity V p, discharge Q p of pipe flow and radius r of soil pipe, great similarity was found between simulated and observed values. Particularly, the simulated length of soil pipes reflects the great difference among soil pipes as a result of its different position in the soil profile. The length values of 4 soil pipes were estimated to be 98 1%, 27 6%, 11 0% and 3 0% of the longest distance of the catchment, respectively. As a special case of water movement, soil pipe flow follows Darcy Weisbach law. Discharge of pipe flow is much greater than infiltration discharge in common. Only when the depth of groundwater is more than the diameter of soil pipe and water layer submerges soil pipes during rainfall, may pipe flow occur. Under these circumstances, discharge of pipe flow is directly proportional to the depth of groundwater.展开更多
Populus tomentosa cuttings were treated with 1mmol·L -1 , 5mmol·L -1 , 10mmol·L -1 or 15mmol·L -1 of CaCl 2 for 1\|7 d, respectively, for studying the effects of different conc...Populus tomentosa cuttings were treated with 1mmol·L -1 , 5mmol·L -1 , 10mmol·L -1 or 15mmol·L -1 of CaCl 2 for 1\|7 d, respectively, for studying the effects of different concentrations of CaCl 2 on freezing resistance. Results indicated that 10?mmol·L -1 of CaCl 2 has greater effect than other concentrations on the enhancement of freezing resistance, and the optimum time of pretreatment was 5?d. In addition, cuttings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 of CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 05?mmol·L -1 of CaM antagonist CPZ or 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3 The changes in CaM and freezing resistance of all cuttings were investigated. The results showed that cold acclimation at -3℃ increased CaM content and decreased the minimum temperature for 100% survival. The CaCl 2 pretreatment enhanced the effect of cold acclimation and obviously increased CaM content and decreased the minimum temperature for 100% survival, but this effect was strongly inhibited by the EGTA, CPZ or LaCl 3 It is concluded that the effect of CaCl 2 on freezing resistance is associated with its concentration and time of pretreatment, Ca 2+ CaM may be involved in the induction of freezing resistance of the cuttings.展开更多
Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ ...Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.展开更多
Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger...Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.展开更多
Through one hundred years of adaptation in China, The Acer negundo Linn. (as hlea f maple), originally grown in North America, has developed morphological charact er istics to cope the severe situation of its habitats...Through one hundred years of adaptation in China, The Acer negundo Linn. (as hlea f maple), originally grown in North America, has developed morphological charact er istics to cope the severe situation of its habitats. Two ecotypes from different vegetation belts in the ecotone of eastern Inner Mongolia Autonomous Region wer e detected. Although there was no macroscopic difference, contrasting micro-ch a racteristics had been observed between the two sources. The leaves from more dro ught and warmer zone were found with more trichomes, more and smaller stomata, l arger subsidiary cells, thicker palisade and flatter epidermal wax than those of leaves from the more humid and colder zone. Microstructures of the leaves from xeric sources can more effectively reduce leaf temperature and conserving water than those of mesic sources. Intra-species difference of the two ecotypes is th e result of adaptation in different environments. This should be considered when characteristics of foliage are used as characters of species in taxonomy.展开更多
The importance of water on rattan is described in the paper. Up to date, some researches have witnessed that rain determines the distribution and the number of species of rattan in wild, and is helpful to dispersal of...The importance of water on rattan is described in the paper. Up to date, some researches have witnessed that rain determines the distribution and the number of species of rattan in wild, and is helpful to dispersal of rattan seeds with slope. Rattan plays a role in intercepting the splash effect of rain and improves the water holding capacity of soil. The viability of rattan seed decreases quickly with the decrease of water content of seeds, hence to reduce the germination rate of seeds. With enough water, rattan grows more quickly in terms of leaf, leaf area, and stem. Water stress improves the ratio of root to shoot of rattan, and humidity plays a role in the distribution and growth of rattan.展开更多
Microclimate characteristics and related environmental energy mechanisms were examined based on the long term located observations in the mature, thinned and young Cunninghamia lanceolata plantation ecosystems in...Microclimate characteristics and related environmental energy mechanisms were examined based on the long term located observations in the mature, thinned and young Cunninghamia lanceolata plantation ecosystems in western Hunan Province, China. The results show that the mature plantation ecosystem can improve the microclimate significantly by regulating the amount and spatial distribution of environmental energy, which delineates the pattern of the microclimate in forest ecosystems in the process of ecological restoration. Compared with the young plantation, the mature plantation ecosystem decreased annual mean air temperature by 0 4℃. The maximum decrease in monthly mean air temperature was 2 3℃. The mature plantation ecosystem decreased annual mean ground temperature by 1 2℃ with a maximum decrease in monthly mean ground temperature of 2 3℃. Mainly due to the dense canopy, the mature forest ecosystem regulates the distribution of radiation energy, and expenditure ratios of heat budget and principal energy components to decrease temperature or make it even.展开更多
A multi functional wood dehumidification dryer has been developed recently. The dryer can carry out air dehumidification by using only one refrigeration system, in which heat supplying and moisture air are exhausted ...A multi functional wood dehumidification dryer has been developed recently. The dryer can carry out air dehumidification by using only one refrigeration system, in which heat supplying and moisture air are exhausted by changing the position of several air dampers. In this paper, the work principles and characteristics of the dehumidification dryer are introduced, and the testing results about the thermal engineering performance of the dehumidification dryer, as well as the experiment results of drying several species of lumbers are expounded.展开更多
Based on the definition of desertification adopted by United Nations Convention to Combat Desertification in Those Countries Experiencing Serious Drought and/or Desertification,particularly in Africa, first,the pat...Based on the definition of desertification adopted by United Nations Convention to Combat Desertification in Those Countries Experiencing Serious Drought and/or Desertification,particularly in Africa, first,the patterns of desertification in China are introduced.Second,desertification assessment indicator system that used in China is presented.It includes:(1)climate classification;(2)classification of land use;(3)classification of desertification patterns which include wind erosion,water erosion,salinization,and grassland degradation;(4)grading index of desertification that includes grading index of desertification caused by wind erosion,desertification caused by water erosion,desertification caused by salinization,indicators of grassland degradation classification.At last,some detailed factors that can be used as indicators in desertification assessment are discussed.展开更多
The CAR(Constant Allometric Ratio) and VAR(Variable Allometric Ratio) models wer e two basic biomass models most widely used in research and applications. Re\|sa mpling and sign test were employed in this paper to com...The CAR(Constant Allometric Ratio) and VAR(Variable Allometric Ratio) models wer e two basic biomass models most widely used in research and applications. Re\|sa mpling and sign test were employed in this paper to compare these two models for their parameters' stabilities and their predictions. Research showed that the C AR model would give more stable parameter and more accurate estimation than the VAR model.展开更多
A new species, Spirulina (Arthrospira) bayannurensis B. Sh. Li et C. Qiao sp. nov. was firstly collected from the Lake of Bayannur, Mu Us Sandy Land, Inner Mongolia Autonomous Region in China on April 30, 199...A new species, Spirulina (Arthrospira) bayannurensis B. Sh. Li et C. Qiao sp. nov. was firstly collected from the Lake of Bayannur, Mu Us Sandy Land, Inner Mongolia Autonomous Region in China on April 30, 1996 The morphological characteristics of the new species are described and illustrated by light micrographs, scanning and transmission electron micrographs. The type specimen of this new species is kept in Herbarium of Inner Mongolia Agricultural University.展开更多
Based on the measurement of monthly litterfall and their gross calor ic values, the seasonal dynamics of energy return through litterfall were determ ined in a pure and a mixed T. odorum (Tsoongiodendron odorum Chun) ...Based on the measurement of monthly litterfall and their gross calor ic values, the seasonal dynamics of energy return through litterfall were determ ined in a pure and a mixed T. odorum (Tsoongiodendron odorum Chun) forests with Ch inese fir (Cunninghamia lanceolata (Lamb.) Hook.) in Sanming, Fujian Provinc e. Annual ene rgy return through litterfall was estimated as 12.648×10 6J·m -2 for the mixed fo rest, being 4 2% higher than that of the pure forest, and a large proportion of the energy return comprised leaf litter. The conversion efficiency of solar rad i ation energy into litterfall was 0 56% for mixed forest and 0 54% for pure for es t, respectively. The monthly energy flux in litterfall of Chinese fir showed a t hree-apex curve, peaked in March, August and December, respectively, which was s imilar to that in various fractions of leaf, twig, flower and fruit litter. The consistency in monthly patterns among different litter fractions of Chinese fir was attributed to their solid connections all the while. The monthly energy flux in litterfall of T. odorum culminated in January, May and August, the same was true for its leaf and twig litter. However, energy flux in flower litter only oc curred during March to May and that in fruit litter appeared in January and Marc h. The monthly dynamics of energy flux through litterfall of the two forests wer e both determined by their respective litterfall pattern of Chinese fir. Seasona l energy flux in litterfall for both mixed and pure forests followed the sequenc e of spring>winter>summer>autumn, but fluctuations in the former were less disti nct than those in the latter.展开更多
This article summarized systematically the previous investigations on t he dielectric relaxation of wood, the main substances and extractives in wood at oven-dry state, and the dielectric relaxation based on the adso...This article summarized systematically the previous investigations on t he dielectric relaxation of wood, the main substances and extractives in wood at oven-dry state, and the dielectric relaxation based on the adsorbed water in w o od cell wall under equilibrium and non-equilibrium state. Moreover, some expect a tions for future research were proposed on this basis. The purpose of this artic le is to provide other researchers an overall understanding about the research i n this region, and further to promote the research onto a new and higher level.展开更多
The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress an...The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings.展开更多
文摘The establishment of high frequency regeneration system is a foundation for Agrobacterium mediated genetic transformation. In this work, several important factors influencing the efficiency of regeneration of plants, such as concentration of plant growth regulators, leaf explant orientation, leaf growth sequence and leaf segment, were studied. The results indicated that the differentiation rate of adventitious shoots was 90% on basal MS medium only supplemented with 1 5?mg·L -1 BA (6 benzyladenine) and reached the highest level(95%) when 1 0?mg·L -1 BA and 0 3?mg·L -1 NAA (naphthaleneacetic acid) were added to MS medium. 90% of differentiation rate of adventitious roots were obtained when 0 3?mg·L -1 NAA was only added to MS medium. It was also found that more adventitious shoots were regenerated from the lower segment of leaf (with petiole) than the other segments, the number of adventitious shoots decreased from top to base of leaf growth sequence and the percentage of adventitious shoot induction with adaxial side downward was higher than that with adaxial side upward.
文摘The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.
文摘Expression of rol genes from Ri plasmid of Agrobacterium rhizogenes not only leads to the excessive formation of adventitious roots, but also exhibits various genetically modified characteristics that have broad prospects for the application of plant genetic improvement. Since the 1980s of the last century, much progress has been made in the studies of A. rhizogenes, in particular the agropine type Ri plasmid rol genes and their applications for plant genetic improvement, which involves the structure and function of Ri plasmid, the characters of rol genes, the influence of rol genes expression on plants growth and development, and the applications of rol genes for genetic improvement of forest tree. In this paper, the advances in this field are reviewed and the existing problems about the application of rol genes for genetic improvement of forest tree are also discussed.
文摘Selection of fire resistant tree species for the southwestern China and the planting of those species can effectively prevent large area's fire damage. In this paper the components and flammability of leaves, twigs and barks of 12 tree species in the mountain area of southwestern China have been tested and analyzed in the laboratory. The test and analysis indicate the results as follows:(1) for all the tree species, the fire resistance of leaves is much weaker than that of twigs and barks, and the broad leaves are stronger than those of conifers in fire resistance. (2) Heat value, moisture, ignition point and ash content are main indexes to affect fire resistance. Heat value relates to lignose content and benzene ethanol extractive content linearly.(3) Of all the 12 tree species, Schima superba,Castanopsis hystrix, Myrica rubra have the strongest resistance to fire; Machilus pauhoi, Michelia macclurei, Mytilaria laosensis, Camellia olifera and Manglietia tenuipes are relatively strong in fire resistance, and Lithocapus thalassica, Tsoongiodendron odorum, Cunninghamia lanceolata and Pinus massoniana are weak in fire resistance.
文摘On the basis of natural conditions of lack of rainfall on the Loess Plateau, this paper presents a method of design and construction in rainwater harvesting system for afforestation, which is suitable to the regions of 300 600?mm rainfall. The system consists of micro catchments of various forms and sizes that include planting areas, where trees are planted and trapped runoff, and contributing areas, where overland flow are produced by rainfall. The design is based on rainfall, runoff coefficients of contributing areas, evapotranspiration of trees and soil surface, water deficiency of soil, and environmental capacity of precipitation in the region, and so on. Runoff coefficient of contributing areas with YJG (organic silicon chemical treatment), compacted surface soil slope and natural slope micro catchments, are 0 8 0 89, 0 23 0 36, and 0 08 0 10 respectively. According to the Penman method, the soil water deficiency varies from 50?mm to 300?mm. In the region of 400?mm precipitation, the contributing area treated with YJG is 3 4?m 2 for timber forests, 8 10?m 2 for cash trees respectively; the contributing area treated with compacted soil surface is 6 8?m\+2 for timber forests, 10 12?m 2 for cash trees respectively; the contributing area of natural slope is 8 10?m 2 for timber forests, 12 15?m 2 for cash trees respectively. Transpiration from trees of micro catchment in YJG, compacted surface soil and natural slope treatment is by 47 65% 53 31%, 24 10% 36 93%, and 18 65% 29 55% of total rainfall (rainfall and harvested rainwater) respectively after the system was applied in the region. This system, which has been widely practising on the Loess Plateau, is now known as runoff forestry.
文摘The property of major cellulases from the guts of Anoplophora glabripennis larvae have been characterized. The optimal temperatures of both β 1,4 glucosidase (β glucosidase) and endo β 1,4 endoglucanase (endoglucanase, Cx) are 40℃. The β glucosidase was optimally active at pH 4\^8, while the optimal activity of the endoglucanase occurred at pH 4 4 5 6 The endoglucanase was active with a wide range of pH and temperature, the levels of activity from 25℃ to 50℃ were more than 80%, and the activity remained 60% between pH 3 2 and pH 7 2. The endoglucanase exhibited higher thermal stability than β glucosidase. Both enzymes lose their activities by heat treatment at 60℃. Two isozymes of endoglucanase were detected in sodium carboxymethylcellulose polyacrymide gels (CMC gel) by chemical colorization, and purified by elution from the gel slices. The molecular weights of the two isozymes were estimated as 26kD and 39kD respectively. Moreover molecular characteristics of the two isozymes are currently underway.
文摘It is well known that, in most cases, soil water doesn't move in the form of laminar flow as described by Darcy law. Only when Reynolds number ( Re ) is no more than 10, does water movement follow Darcy law. A soil profile with 2 9 m long and 2 13 2 60 m deep was excavated on a lower slope located at Zigui County, Hubei Province, China. Field observation found that soil pipes were mainly distributed in the transient layer between horizon B with higher degree of granite weathering and horizon C with lower degree of granite weathering. At the foot of the slope, about 5 7 soil pipes per meter were observed along the vertical direction of the slope. The observed results, obtained by continuous observation of soil pipes and pipe flow processes at granite slope for many rainfall events, indicate that the relationship between velocity of pipe flow and hydraulic gradient along the pipe is parabolic rather than linear. Based on the investigated data of soil, landform, and land use etc., combined with observed data of pipe flow derived from many rainfall events, a pipe flow model was developed. For velocity V p, discharge Q p of pipe flow and radius r of soil pipe, great similarity was found between simulated and observed values. Particularly, the simulated length of soil pipes reflects the great difference among soil pipes as a result of its different position in the soil profile. The length values of 4 soil pipes were estimated to be 98 1%, 27 6%, 11 0% and 3 0% of the longest distance of the catchment, respectively. As a special case of water movement, soil pipe flow follows Darcy Weisbach law. Discharge of pipe flow is much greater than infiltration discharge in common. Only when the depth of groundwater is more than the diameter of soil pipe and water layer submerges soil pipes during rainfall, may pipe flow occur. Under these circumstances, discharge of pipe flow is directly proportional to the depth of groundwater.
文摘Populus tomentosa cuttings were treated with 1mmol·L -1 , 5mmol·L -1 , 10mmol·L -1 or 15mmol·L -1 of CaCl 2 for 1\|7 d, respectively, for studying the effects of different concentrations of CaCl 2 on freezing resistance. Results indicated that 10?mmol·L -1 of CaCl 2 has greater effect than other concentrations on the enhancement of freezing resistance, and the optimum time of pretreatment was 5?d. In addition, cuttings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 of CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 05?mmol·L -1 of CaM antagonist CPZ or 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3 The changes in CaM and freezing resistance of all cuttings were investigated. The results showed that cold acclimation at -3℃ increased CaM content and decreased the minimum temperature for 100% survival. The CaCl 2 pretreatment enhanced the effect of cold acclimation and obviously increased CaM content and decreased the minimum temperature for 100% survival, but this effect was strongly inhibited by the EGTA, CPZ or LaCl 3 It is concluded that the effect of CaCl 2 on freezing resistance is associated with its concentration and time of pretreatment, Ca 2+ CaM may be involved in the induction of freezing resistance of the cuttings.
文摘Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.
文摘Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.
文摘Through one hundred years of adaptation in China, The Acer negundo Linn. (as hlea f maple), originally grown in North America, has developed morphological charact er istics to cope the severe situation of its habitats. Two ecotypes from different vegetation belts in the ecotone of eastern Inner Mongolia Autonomous Region wer e detected. Although there was no macroscopic difference, contrasting micro-ch a racteristics had been observed between the two sources. The leaves from more dro ught and warmer zone were found with more trichomes, more and smaller stomata, l arger subsidiary cells, thicker palisade and flatter epidermal wax than those of leaves from the more humid and colder zone. Microstructures of the leaves from xeric sources can more effectively reduce leaf temperature and conserving water than those of mesic sources. Intra-species difference of the two ecotypes is th e result of adaptation in different environments. This should be considered when characteristics of foliage are used as characters of species in taxonomy.
文摘The importance of water on rattan is described in the paper. Up to date, some researches have witnessed that rain determines the distribution and the number of species of rattan in wild, and is helpful to dispersal of rattan seeds with slope. Rattan plays a role in intercepting the splash effect of rain and improves the water holding capacity of soil. The viability of rattan seed decreases quickly with the decrease of water content of seeds, hence to reduce the germination rate of seeds. With enough water, rattan grows more quickly in terms of leaf, leaf area, and stem. Water stress improves the ratio of root to shoot of rattan, and humidity plays a role in the distribution and growth of rattan.
文摘Microclimate characteristics and related environmental energy mechanisms were examined based on the long term located observations in the mature, thinned and young Cunninghamia lanceolata plantation ecosystems in western Hunan Province, China. The results show that the mature plantation ecosystem can improve the microclimate significantly by regulating the amount and spatial distribution of environmental energy, which delineates the pattern of the microclimate in forest ecosystems in the process of ecological restoration. Compared with the young plantation, the mature plantation ecosystem decreased annual mean air temperature by 0 4℃. The maximum decrease in monthly mean air temperature was 2 3℃. The mature plantation ecosystem decreased annual mean ground temperature by 1 2℃ with a maximum decrease in monthly mean ground temperature of 2 3℃. Mainly due to the dense canopy, the mature forest ecosystem regulates the distribution of radiation energy, and expenditure ratios of heat budget and principal energy components to decrease temperature or make it even.
文摘A multi functional wood dehumidification dryer has been developed recently. The dryer can carry out air dehumidification by using only one refrigeration system, in which heat supplying and moisture air are exhausted by changing the position of several air dampers. In this paper, the work principles and characteristics of the dehumidification dryer are introduced, and the testing results about the thermal engineering performance of the dehumidification dryer, as well as the experiment results of drying several species of lumbers are expounded.
文摘Based on the definition of desertification adopted by United Nations Convention to Combat Desertification in Those Countries Experiencing Serious Drought and/or Desertification,particularly in Africa, first,the patterns of desertification in China are introduced.Second,desertification assessment indicator system that used in China is presented.It includes:(1)climate classification;(2)classification of land use;(3)classification of desertification patterns which include wind erosion,water erosion,salinization,and grassland degradation;(4)grading index of desertification that includes grading index of desertification caused by wind erosion,desertification caused by water erosion,desertification caused by salinization,indicators of grassland degradation classification.At last,some detailed factors that can be used as indicators in desertification assessment are discussed.
文摘The CAR(Constant Allometric Ratio) and VAR(Variable Allometric Ratio) models wer e two basic biomass models most widely used in research and applications. Re\|sa mpling and sign test were employed in this paper to compare these two models for their parameters' stabilities and their predictions. Research showed that the C AR model would give more stable parameter and more accurate estimation than the VAR model.
文摘A new species, Spirulina (Arthrospira) bayannurensis B. Sh. Li et C. Qiao sp. nov. was firstly collected from the Lake of Bayannur, Mu Us Sandy Land, Inner Mongolia Autonomous Region in China on April 30, 1996 The morphological characteristics of the new species are described and illustrated by light micrographs, scanning and transmission electron micrographs. The type specimen of this new species is kept in Herbarium of Inner Mongolia Agricultural University.
基金SupportedbytheFoundationofPost doctoralResearchof China (2 0 0 0F0 0 4 )
文摘Based on the measurement of monthly litterfall and their gross calor ic values, the seasonal dynamics of energy return through litterfall were determ ined in a pure and a mixed T. odorum (Tsoongiodendron odorum Chun) forests with Ch inese fir (Cunninghamia lanceolata (Lamb.) Hook.) in Sanming, Fujian Provinc e. Annual ene rgy return through litterfall was estimated as 12.648×10 6J·m -2 for the mixed fo rest, being 4 2% higher than that of the pure forest, and a large proportion of the energy return comprised leaf litter. The conversion efficiency of solar rad i ation energy into litterfall was 0 56% for mixed forest and 0 54% for pure for es t, respectively. The monthly energy flux in litterfall of Chinese fir showed a t hree-apex curve, peaked in March, August and December, respectively, which was s imilar to that in various fractions of leaf, twig, flower and fruit litter. The consistency in monthly patterns among different litter fractions of Chinese fir was attributed to their solid connections all the while. The monthly energy flux in litterfall of T. odorum culminated in January, May and August, the same was true for its leaf and twig litter. However, energy flux in flower litter only oc curred during March to May and that in fruit litter appeared in January and Marc h. The monthly dynamics of energy flux through litterfall of the two forests wer e both determined by their respective litterfall pattern of Chinese fir. Seasona l energy flux in litterfall for both mixed and pure forests followed the sequenc e of spring>winter>summer>autumn, but fluctuations in the former were less disti nct than those in the latter.
文摘This article summarized systematically the previous investigations on t he dielectric relaxation of wood, the main substances and extractives in wood at oven-dry state, and the dielectric relaxation based on the adsorbed water in w o od cell wall under equilibrium and non-equilibrium state. Moreover, some expect a tions for future research were proposed on this basis. The purpose of this artic le is to provide other researchers an overall understanding about the research i n this region, and further to promote the research onto a new and higher level.
文摘The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings.
