Sprague-Dawley rats were subjected to traumatic spinal cord injury using Allen's method. Regional concentrations of excitatory amino acid (EAA) were assessed by high-performance liquid chromatography, and regional...Sprague-Dawley rats were subjected to traumatic spinal cord injury using Allen's method. Regional concentrations of excitatory amino acid (EAA) were assessed by high-performance liquid chromatography, and regional water and ion content were measured by the atomic absorption spectrophotometry at various times after intrathecal administration of different dosage of 3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP),a specific antagonist of N-methyl-D-aspartame (NMDA) receptor,to rat at 48 h posttrauma.The results showed that concentrations of glutamate and aspartate were elevated rapidly at 15, 30 min and were correlated positively with the severity of injury. In the injured segment there was a significant increase in water, Na+ ,Ca++ content and a decrease in K+ , Mg++. Whereas in CPP treated groups they had a statistically significant return, which was related to the dosage and time of administration. This effect of CPP suggests that ion and water content were related to overactivation of NMDA receptor. Excessive high levels of EAA may be involved in secondary tissue damage following spinal cord injury.展开更多
文摘Sprague-Dawley rats were subjected to traumatic spinal cord injury using Allen's method. Regional concentrations of excitatory amino acid (EAA) were assessed by high-performance liquid chromatography, and regional water and ion content were measured by the atomic absorption spectrophotometry at various times after intrathecal administration of different dosage of 3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP),a specific antagonist of N-methyl-D-aspartame (NMDA) receptor,to rat at 48 h posttrauma.The results showed that concentrations of glutamate and aspartate were elevated rapidly at 15, 30 min and were correlated positively with the severity of injury. In the injured segment there was a significant increase in water, Na+ ,Ca++ content and a decrease in K+ , Mg++. Whereas in CPP treated groups they had a statistically significant return, which was related to the dosage and time of administration. This effect of CPP suggests that ion and water content were related to overactivation of NMDA receptor. Excessive high levels of EAA may be involved in secondary tissue damage following spinal cord injury.
