To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subseq...To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.展开更多
文摘To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.
文摘为了通过动物实验评价植物乳杆菌(Lactiplantibacillus plantarum)CCFM8661的免疫调节功能,作者采用120只雄性BALB/c小鼠,分别灌胃植物乳杆菌CCFM8661(低、中、高3个剂量)或安慰剂30 d。之后采用以下方法综合评估植物乳杆菌CCFM8661调节小鼠免疫力的功效:小鼠脾淋巴细胞转化实验及迟发型变态反应(delayed type hypersensitivity,DTH)实验评价小鼠细胞免疫功能;血清溶血素生成实验及抗体生成细胞检测实验评价小鼠体液免疫功能;碳廓清实验及腹腔巨噬细胞吞噬荧光微球实验评价小鼠单核-巨噬细胞功能;自然杀伤(nature-killer,NK)细胞活性检测评估小鼠NK细胞功能。结果显示,植物乳杆菌CCFM8661可明显增强小鼠迟发型变态反应的耳肿胀程度、提升小鼠脾淋巴细胞的增殖水平、促进小鼠血清溶血素的生成、提高小鼠腹腔巨噬细胞的吞噬能力及机体碳处理能力。研究结果表明植物乳杆菌CCFM8661具有增强小鼠免疫力的作用,为其实际应用提供理论依据。
