期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
MicroRNA-9影响涎腺腺样囊性癌侵袭能力的分子机制研究 被引量:3
1
作者 徐川 李亚楠 +4 位作者 谯丹 古欢 钟罗 吴键 于涛 《口腔医学研究》 CAS 北大核心 2019年第6期563-567,共5页
目的:探讨miR-9对人涎腺腺样囊性癌细胞侵袭的影响及其相关分子机制。方法:检测ACC-2,ACC-M细胞中miR-9表达,构建慢病毒miR-9过表达载体,转染ACC-2,ACC-M肿瘤细胞,实时荧光定量PCR检测细胞中 miR-9的表达水平。Transwell方法检测转染mi... 目的:探讨miR-9对人涎腺腺样囊性癌细胞侵袭的影响及其相关分子机制。方法:检测ACC-2,ACC-M细胞中miR-9表达,构建慢病毒miR-9过表达载体,转染ACC-2,ACC-M肿瘤细胞,实时荧光定量PCR检测细胞中 miR-9的表达水平。Transwell方法检测转染miR-9前后ACC-M细胞侵袭转移能力的变化,Western 免疫印迹检测转染前后ACC-M细胞中基质金属蛋白酶家族蛋白(MMP)及数个肿瘤相关信号通路蛋白的表达情况,采用 SPSS 16.0 软件包对数据进行统计学分析。结果:相比于ACC-2,ACC-M细胞中出现了显著的miR-9的表达下调( P <0.01)。携带miR-9的慢病毒转染细胞后均显著提高了2株细胞中miR-9的表达并显著降低了ACC-M细胞的侵袭能力( P <0.01)。同时,转染miR-9后显著降低了MMP-2及MMP-9蛋白表达及AKT蛋白的磷酸化水平。结论:上调miR-9 的表达可显著抑制ACC-M细胞的侵袭能力,其机制可能与AKT信号通路介导的MMP-2,9蛋白的表达下调密切相关。 展开更多
关键词 腺样囊性癌 微小RNA 基质金属蛋白酶 AKT信号通路
在线阅读 下载PDF
Stimuli-responsive nano-reserveriors drug delivery systems based on inorganic nanoparticles
2
作者 Kaiyong Cai zhong luo Yan Hu 《医用生物力学》 EI CAS CSCD 北大核心 2013年第S1期5-6,共2页
The convergence of nanotechnology,material science,biology and medicine promotes the rapid development of nano-biomedicine,which plays an important role in relieving the pain of patients and improving the health of hu... The convergence of nanotechnology,material science,biology and medicine promotes the rapid development of nano-biomedicine,which plays an important role in relieving the pain of patients and improving the health of human,in particular of cancer therapy.Construction of stimuli-responsive controlled release system for targeted drug delivery to specific cells is essentially important for cancer therapy since most chemotherapeutics are severely toxic<sup>[1]</sup>.During past decades,magnetic nanoparticles(MNPs)and mesoporous silica nanoparticles(MSNs)attracted much attention in the related field,due to their good biocompatibility。 展开更多
关键词 INORGANIC nanotechnology RESPONSIVE silica TARGETED HOLLOW CYCLODEXTRIN probes redox essentially
在线阅读 下载PDF
Microenvironmental biomaterials
3
作者 Kaiyong Cai Yan Hu zhong luo 《医用生物力学》 EI CAS CSCD 2009年第S1期49-49,共1页
Biomaterials play essential role in regenerative medicine and tissue engineering,which providing a provisional three-dimensional(3D)microenvironments to interact biophysically and/or biochemically with cells to guide ... Biomaterials play essential role in regenerative medicine and tissue engineering,which providing a provisional three-dimensional(3D)microenvironments to interact biophysically and/or biochemically with cells to guide cellular performance[1].It thus spatially and temporally regulates complex cellular process of tissue formation,function and regeneration. 展开更多
关键词 GENE Microenvironmental biomaterials
在线阅读 下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部