研究了藏药无茎芥[Pegaeophyton scapiflorum(Hook. f. et Thoms.) Marq. et Shaw]挥发油的化学成分。采用同时蒸馏萃取法(SDE)提取无茎芥中的挥发油,使用气质联用技术获得总离子流图,各色谱峰相应的质谱图经过NIST2011标准谱库检索定性...研究了藏药无茎芥[Pegaeophyton scapiflorum(Hook. f. et Thoms.) Marq. et Shaw]挥发油的化学成分。采用同时蒸馏萃取法(SDE)提取无茎芥中的挥发油,使用气质联用技术获得总离子流图,各色谱峰相应的质谱图经过NIST2011标准谱库检索定性,并采用峰面积归一化法进行定量分析,计算各成分的相对百分含量。从无茎芥挥发油中鉴定出82种物质,主要成分为异硫氰酸酯(26.35%)、有机多硫化物(16.35%)、芳香类化合物(15.78%)、醛酮类(13.27%)、脂肪酸类(7.14%)、腈类(3.71%)、烃类(3.51%)和醇类(3.08%)等。结论:藏药无茎芥挥发油中富含异硫氰酸酯,具有很好的开发利用价值。展开更多
Slug,a member of the Snail family of transcriptional repressors,plays a key role in cancer progression,cellular plasticity,and epithelial to mesenchymal transition(EMT).Slug is a fast turnover protein and its stabilty...Slug,a member of the Snail family of transcriptional repressors,plays a key role in cancer progression,cellular plasticity,and epithelial to mesenchymal transition(EMT).Slug is a fast turnover protein and its stabilty is controlled by post-translational modifications.Here,we identified that Slug is acetylated by acetyltransferase CREB-binding protein(CBP)in breast cancer cells.CBP directly interacts with the C-terminal domain of Slug through its catalytic histone acetyltransferase(HAT)domain,leading to acetylation of Slug at lysines 166 and 211.展开更多
Kindlin-2 is widely expressed in striated and smooth muscle cells(SMC).However,the function of Kindlin-2 in smooth muscle remains elusive.Here,we demonstrate that depletion of Kindlin-2 encoding gene F ermt2 in smooth...Kindlin-2 is widely expressed in striated and smooth muscle cells(SMC).However,the function of Kindlin-2 in smooth muscle remains elusive.Here,we demonstrate that depletion of Kindlin-2 encoding gene F ermt2 in smooth muscle leads to apoptosis,downregulates the key components of SMC,impairs smooth muscle development and finally embryonic death at E14.5.Tamoxif en-induced Kindlin-2 knockout in adult mice show ed decreased blood pressure,intestinal hypoperistalsis,and eventully died of intestinal obstruction.Loss of Kindlin-2 also leads to shortened myofilament,decreased Myh1l,a-SMA and CNN,damaged ultrastructure and jeopardized contractility of the smooth muscle in iKO mice.Mechanistically,loss of Kindlin-2 impairs Ca+influx in mouse primary vascular smooth muscle cells(PVSMC)by downregulating the expression of calcium bi nding protein S100A14 and STIM1.展开更多
文摘研究了藏药无茎芥[Pegaeophyton scapiflorum(Hook. f. et Thoms.) Marq. et Shaw]挥发油的化学成分。采用同时蒸馏萃取法(SDE)提取无茎芥中的挥发油,使用气质联用技术获得总离子流图,各色谱峰相应的质谱图经过NIST2011标准谱库检索定性,并采用峰面积归一化法进行定量分析,计算各成分的相对百分含量。从无茎芥挥发油中鉴定出82种物质,主要成分为异硫氰酸酯(26.35%)、有机多硫化物(16.35%)、芳香类化合物(15.78%)、醛酮类(13.27%)、脂肪酸类(7.14%)、腈类(3.71%)、烃类(3.51%)和醇类(3.08%)等。结论:藏药无茎芥挥发油中富含异硫氰酸酯,具有很好的开发利用价值。
文摘Slug,a member of the Snail family of transcriptional repressors,plays a key role in cancer progression,cellular plasticity,and epithelial to mesenchymal transition(EMT).Slug is a fast turnover protein and its stabilty is controlled by post-translational modifications.Here,we identified that Slug is acetylated by acetyltransferase CREB-binding protein(CBP)in breast cancer cells.CBP directly interacts with the C-terminal domain of Slug through its catalytic histone acetyltransferase(HAT)domain,leading to acetylation of Slug at lysines 166 and 211.
文摘Kindlin-2 is widely expressed in striated and smooth muscle cells(SMC).However,the function of Kindlin-2 in smooth muscle remains elusive.Here,we demonstrate that depletion of Kindlin-2 encoding gene F ermt2 in smooth muscle leads to apoptosis,downregulates the key components of SMC,impairs smooth muscle development and finally embryonic death at E14.5.Tamoxif en-induced Kindlin-2 knockout in adult mice show ed decreased blood pressure,intestinal hypoperistalsis,and eventully died of intestinal obstruction.Loss of Kindlin-2 also leads to shortened myofilament,decreased Myh1l,a-SMA and CNN,damaged ultrastructure and jeopardized contractility of the smooth muscle in iKO mice.Mechanistically,loss of Kindlin-2 impairs Ca+influx in mouse primary vascular smooth muscle cells(PVSMC)by downregulating the expression of calcium bi nding protein S100A14 and STIM1.
