Titanium alloy has the advantages of high strength,strong corrosion resistance,excellent high and low temperature mechanical properties,etc.,and is widely used in aerospace,shipbuilding,weapons and equipment,and other...Titanium alloy has the advantages of high strength,strong corrosion resistance,excellent high and low temperature mechanical properties,etc.,and is widely used in aerospace,shipbuilding,weapons and equipment,and other fields.In recent years,with the continuous increase in demand for medium-thick plate titanium alloys,corresponding welding technologies have also continued to develop.Therefore,this article reviews the research progress of deep penetration welding technology for medium-thick plate titanium alloys,mainly covering traditional arc welding,high-energy beam welding,and other welding technologies.Among many methods,narrow gap welding,hybrid welding,and external energy field assistance welding all contribute to improving the welding efficiency and quality of medium-thick plate titanium alloys.Finally,the development trend of deep penetration welding technology for mediumthick plate titanium alloys is prospected.展开更多
OBJECTIVE Granulin A(GRN A),a cytokinesis protein,is derived from proteolysis of progranulin.The previous study in our laboratory has shown that GRN A is able to inhibit cancer cell growth significantly.This study aim...OBJECTIVE Granulin A(GRN A),a cytokinesis protein,is derived from proteolysis of progranulin.The previous study in our laboratory has shown that GRN A is able to inhibit cancer cell growth significantly.This study aimed to investigate the effect of combination of GRN A and cisplatin on in vitro and in vivo on the growth of hepatocellular carcinoma.METHODS The in vitro and in vivo antitumor effects of combination of GRN A and Cisplatin were evaluated with MTS assay and subcuta.neous transplantation tumor model.Chou-Talalay method was used to calculate the combination index(CI).Colony formation assay and flow cytometry were used to detect the effects of GRN A on apoptosis.The expression of apoptosis-related proteins were detected by Western blot.RESULTS MTS assay showed that GRN A significantly inhibit hepatocellular carcinoma cells growth with the IC50 of 5.6 μmol·L^(-1),and GRN A combined with cisplatin synergistically inhibit hepatocellular carcinoma proliferation,with the CI<1.The colony-formation assay showed that GRN A significantly enhanced the inhibitory effects of cisplatin on cellular anchorage-independent growth.Flow cytometry showed that GRN A combined with cisplatin synergistically induced apoptosis,with the apoptotic rates of 5.87%,32.74%,35.67% and 67.15% in control,GRN A,Cisplatin,and combination of GRN A and Cisplatin groups,respectively.Western blot confirmed that the two drugs synergistically changed the expressions of proteins related to apoptosis.In vivo experiment indicated that combination of GRN A and cisplatin significantly suppressed tumor growth compared with single drug treatment groups.CONCLUSION The combination of GRN A and cisplatin resulted in synergistic antitumor effects against hepatocellular carcinoma both in vitro and in vivo.展开更多
OBJECTIVE Zn-doped CuO nanocomposites(Zn-CuO NPs) are novel nanoparticles synthesized by our research group.In this study,we assessed the in vitro and in vivo antitumor effects of Zn-CuO NPS on pancreatic cancer cells...OBJECTIVE Zn-doped CuO nanocomposites(Zn-CuO NPs) are novel nanoparticles synthesized by our research group.In this study,we assessed the in vitro and in vivo antitumor effects of Zn-CuO NPS on pancreatic cancer cells,as well as the potential mechanisms.METHODS MTS assay was used to detect the effects of Zn-CuO NPS on proliferation pancreatic cancer cells(Panc-mia and Aspc-1).The in vivo antitumor effects of Zn-CuO NPs were detected by xenografts model in nude mice.The effects of Zn-CuO NPS on autophagy were detected bytransmission electron microscopy(TEM) andflow cytometry.Autophagy related proteins were detected by Western blotting.RESULTS Zn-CuO NPS significantly inhibited the proliferation of Panc-mia cells and Aspc-1 cells.In vivo experi.ments showed that Zn-CuO NPS significantly inhibited the tumor growth in nude mice without affecting the body weight of the mice.TEM and flow cytometry showed that Zn-CuO NPS induced autophagy,and significantly increased the number of autophagosome.Western Blot showed that Zn-CuO NPS alterd the expression of autophagy related proteins,such as AMPK,mTORand Beclin-1.Also,AMPK inhibitor could significantly reduce Zn-CuO NPS-induced autophagy pathwayas analyzed byWestern blotting.CONCLUSION The findings suggested that Zn-doped CuO nanocomposites inhibited the in vitro and in vivo growth of pancreatic cancer by inducing autophagy through AMPK/mTOR pathway.展开更多
Being as unique nonlinear components of block ciphers,substitution boxes（S-boxes） directly affect the security of the cryptographic systems.It is important and difficult to design cryptographically strong S-boxes th...Being as unique nonlinear components of block ciphers,substitution boxes（S-boxes） directly affect the security of the cryptographic systems.It is important and difficult to design cryptographically strong S-boxes that simultaneously meet with multiple cryptographic criteria such as bijection,non-linearity,strict avalanche criterion（SAC）,bits independence criterion（BIC）,differential probability（DP） and linear probability（LP）.To deal with this problem,a chaotic S-box based on the artificial bee colony algorithm（CSABC） is designed.It uses the S-boxes generated by the six-dimensional compound hyperchaotic map as the initial individuals and employs ABC to improve their performance.In addition,it considers the nonlinearity and differential uniformity as the fitness functions.A series of experiments have been conducted to compare multiple cryptographic criteria of this algorithm with other algorithms.Simulation results show that the new algorithm has cryptographically strong S-box while meeting multiple cryptographic criteria.展开更多
基金financially supported by the Key Research and Development Program of Ningbo(Grant No.2023Z098)Natural Science Foundation of Inner Mongolia(Grant No.2023MS05040)+1 种基金Shenyang Collaborative Innovation Center Project for Multiple Energy Fields Composite Processing of Special Materials(Grant No.JG210027)Shenyang Key Technology Special Project of The Open Competition Mechanism to Select the Best Solution(Grant Nos.2022210101000827,2022-0-43-048).
文摘Titanium alloy has the advantages of high strength,strong corrosion resistance,excellent high and low temperature mechanical properties,etc.,and is widely used in aerospace,shipbuilding,weapons and equipment,and other fields.In recent years,with the continuous increase in demand for medium-thick plate titanium alloys,corresponding welding technologies have also continued to develop.Therefore,this article reviews the research progress of deep penetration welding technology for medium-thick plate titanium alloys,mainly covering traditional arc welding,high-energy beam welding,and other welding technologies.Among many methods,narrow gap welding,hybrid welding,and external energy field assistance welding all contribute to improving the welding efficiency and quality of medium-thick plate titanium alloys.Finally,the development trend of deep penetration welding technology for mediumthick plate titanium alloys is prospected.
文摘OBJECTIVE Granulin A(GRN A),a cytokinesis protein,is derived from proteolysis of progranulin.The previous study in our laboratory has shown that GRN A is able to inhibit cancer cell growth significantly.This study aimed to investigate the effect of combination of GRN A and cisplatin on in vitro and in vivo on the growth of hepatocellular carcinoma.METHODS The in vitro and in vivo antitumor effects of combination of GRN A and Cisplatin were evaluated with MTS assay and subcuta.neous transplantation tumor model.Chou-Talalay method was used to calculate the combination index(CI).Colony formation assay and flow cytometry were used to detect the effects of GRN A on apoptosis.The expression of apoptosis-related proteins were detected by Western blot.RESULTS MTS assay showed that GRN A significantly inhibit hepatocellular carcinoma cells growth with the IC50 of 5.6 μmol·L^(-1),and GRN A combined with cisplatin synergistically inhibit hepatocellular carcinoma proliferation,with the CI<1.The colony-formation assay showed that GRN A significantly enhanced the inhibitory effects of cisplatin on cellular anchorage-independent growth.Flow cytometry showed that GRN A combined with cisplatin synergistically induced apoptosis,with the apoptotic rates of 5.87%,32.74%,35.67% and 67.15% in control,GRN A,Cisplatin,and combination of GRN A and Cisplatin groups,respectively.Western blot confirmed that the two drugs synergistically changed the expressions of proteins related to apoptosis.In vivo experiment indicated that combination of GRN A and cisplatin significantly suppressed tumor growth compared with single drug treatment groups.CONCLUSION The combination of GRN A and cisplatin resulted in synergistic antitumor effects against hepatocellular carcinoma both in vitro and in vivo.
文摘OBJECTIVE Zn-doped CuO nanocomposites(Zn-CuO NPs) are novel nanoparticles synthesized by our research group.In this study,we assessed the in vitro and in vivo antitumor effects of Zn-CuO NPS on pancreatic cancer cells,as well as the potential mechanisms.METHODS MTS assay was used to detect the effects of Zn-CuO NPS on proliferation pancreatic cancer cells(Panc-mia and Aspc-1).The in vivo antitumor effects of Zn-CuO NPs were detected by xenografts model in nude mice.The effects of Zn-CuO NPS on autophagy were detected bytransmission electron microscopy(TEM) andflow cytometry.Autophagy related proteins were detected by Western blotting.RESULTS Zn-CuO NPS significantly inhibited the proliferation of Panc-mia cells and Aspc-1 cells.In vivo experi.ments showed that Zn-CuO NPS significantly inhibited the tumor growth in nude mice without affecting the body weight of the mice.TEM and flow cytometry showed that Zn-CuO NPS induced autophagy,and significantly increased the number of autophagosome.Western Blot showed that Zn-CuO NPS alterd the expression of autophagy related proteins,such as AMPK,mTORand Beclin-1.Also,AMPK inhibitor could significantly reduce Zn-CuO NPS-induced autophagy pathwayas analyzed byWestern blotting.CONCLUSION The findings suggested that Zn-doped CuO nanocomposites inhibited the in vitro and in vivo growth of pancreatic cancer by inducing autophagy through AMPK/mTOR pathway.
基金supported by the National Natural Science Foundation of China(6060309260975042)
文摘Being as unique nonlinear components of block ciphers,substitution boxes（S-boxes） directly affect the security of the cryptographic systems.It is important and difficult to design cryptographically strong S-boxes that simultaneously meet with multiple cryptographic criteria such as bijection,non-linearity,strict avalanche criterion（SAC）,bits independence criterion（BIC）,differential probability（DP） and linear probability（LP）.To deal with this problem,a chaotic S-box based on the artificial bee colony algorithm（CSABC） is designed.It uses the S-boxes generated by the six-dimensional compound hyperchaotic map as the initial individuals and employs ABC to improve their performance.In addition,it considers the nonlinearity and differential uniformity as the fitness functions.A series of experiments have been conducted to compare multiple cryptographic criteria of this algorithm with other algorithms.Simulation results show that the new algorithm has cryptographically strong S-box while meeting multiple cryptographic criteria.
