研究野生动物栖息地空间分布格局及其与环境因素的关系,对了解该物种适宜生境的空间分布以及生境需求有重要意义。开展珍稀物种原麝(Moschus moschiferus)的空间分布格局研究,可为其栖息地管理和保护提供科学依据。利用MaxEnt模型对陕...研究野生动物栖息地空间分布格局及其与环境因素的关系,对了解该物种适宜生境的空间分布以及生境需求有重要意义。开展珍稀物种原麝(Moschus moschiferus)的空间分布格局研究,可为其栖息地管理和保护提供科学依据。利用MaxEnt模型对陕西黄龙山原麝潜在生境及其主要影响因子进行定量分析,并确定原麝在该区域的潜在分布范围和关键影响因子及其阈值。结果表明,模型预测结果达到优秀水平,原麝潜在分布关键限制因子及其阈值为最干季平均温度<-3.6℃,到道路的距离>4100 m,坡度>71.5°,降水量变化方差为81.5~85.0,最适为83.5,昼夜温差月均值<11.6℃,最适为9.5℃。原麝的适宜区主要分布在宜川县的东南部、黄龙县的东北部及与洛川交界处、韩城的西北部,中等适宜区主要分布在宜川县适宜区周边及县城的西南部、黄龙县适宜区周边及西北部和东部、洛川县的东北部、韩城的西北部适宜区周边。适宜区面积为677.75 km ^(2),中等适宜区为2797.75 km ^(2)。各县域适宜区面积从大到小依次为宜川、韩城、黄龙和洛川,中等适宜区为黄龙、宜川、洛川和韩城。建议加强原麝适宜区的重点保护以及中等适宜区的保护和管理,控制进山人员的数量并进行教育,减少对原麝的干扰和捕杀。同时,建议林业部门近期把宜川县的原麝适宜区调整到陕西黄龙山国家级自然保护区,远期在洛川、黄龙和宜川的交界处成立新的保护区。展开更多
OBJECTIVE SNPs in lnc RNAs may alter the expression or secondary structure of lnc RNAs and then impact their functions.Whether lnc RNA SNPs affect the prognosis of acute myeloid leukemia(AML)remains unknown.To search ...OBJECTIVE SNPs in lnc RNAs may alter the expression or secondary structure of lnc RNAs and then impact their functions.Whether lnc RNA SNPs affect the prognosis of acute myeloid leukemia(AML)remains unknown.To search the association between lnc RNA SNPs and AML outcomes,thirty tag SNPs in GAS5,H19,MALAT1,WT1-as and SRA were genotyped in313 AML patients.METHODS Survival analysis was performed in both AML patients recruited presently and GEO samples.The expression of GAS5 and TP63 was analyzed by real-time quantitative PCR.Dual-luciferase reporter gene assay was used to confirm the interactions between GAS5 rs55829688 and TP63.RESULTS Survival analysis indicated that rs55829688(T>C),located in GAS5 promoter,was significantly associated with the prognosis of AML.The average overall survival(OS)for patients with the rs55829688 CC genotype was significantly shorter than those carrying the rs55829688 T allele(P=0.018).Patients with rs55829688 CC genotype showed higher GAS5 expression in PBMCs than carriers of rs55829688T allele(P=0.025).Rs55829688 CC homozygotes also harbored a longer platelets recovery than those with rs55829688 T allele(P=0.040).In vitro study showed that GAS5 promoter harboring the rs55829688 C al ele showed marginal y increased reporter gene activity(P=0.054),and the promoter activity was increased by TP63 in a dose-dependent manner(P=0.001).Moreover,GAS5 expression was associated with AML OS in the GEO GSE12417 dataset,and GAS5 higher expression predict shorter OS(P=0.011).CONCLUSION Rs55829688 polymorphism could increase GAS5 expression by interacting with TP63 and was associated with worse OS in Chinese AML patients.展开更多
OBJECTIVE To evaluate the association between DNA(cytosine-5)-methyltransferase 3 alpha(DNMT3A)genetic polymorphisms and the disease prognosis of R882 mutation negative acute myeloid leukemia(AML)patients.METHODS DNMT...OBJECTIVE To evaluate the association between DNA(cytosine-5)-methyltransferase 3 alpha(DNMT3A)genetic polymorphisms and the disease prognosis of R882 mutation negative acute myeloid leukemia(AML)patients.METHODS DNMT3A 11 SNPs(rs11695471,rs2289195,rs734693,rs2276598,rs1465825,rs7590760,rs13401241,rs7581217,rs749131,rs41284843 and rs7560488)were genotyped using a MassA RRAY platform or Sanger sequencing method in 317 diagnostic non-FABM3 AML patients without R882 mutation from southern China.AML patients underwent combined chemotherapy with cytarabine and anthracyclines.Overall survival(OS)and Disease-free survival(DFS)as major end points were defined.The prognostic(median OS and DFS)evaluations were performed by Kaplan-Meier curve and Cox′s proportional hazard model.RESULTS We found that the rs2289195 G>A SNP could act as a poor prognostic predictor independently(HR=0.442,P=0.035 for OS;HR=0.431,P=0.031 for DFS),while the rs1465825 T>C SNP and rs7590760 G>C SNP appeared to predict independently poor prognosis for both OS(HR=1.453,P=0.037 for rs1465825;HR=1.584,P=0.063 for rs7590760)and DFS(HR=1.459,P=0.057 for rs1465825;HR=1.965,P=0.017for rs7590760).However,no significant associations between other DNMT3A polymorphisms and prognosis(OS in conjunction with DFS)were observed.CONCLUSION DNMT3A polymorphisms may be potential predictive markers for AML prognosis in R882 mutation negative patients,which might improve prognostic stratification of AML.展开更多
文摘研究野生动物栖息地空间分布格局及其与环境因素的关系,对了解该物种适宜生境的空间分布以及生境需求有重要意义。开展珍稀物种原麝(Moschus moschiferus)的空间分布格局研究,可为其栖息地管理和保护提供科学依据。利用MaxEnt模型对陕西黄龙山原麝潜在生境及其主要影响因子进行定量分析,并确定原麝在该区域的潜在分布范围和关键影响因子及其阈值。结果表明,模型预测结果达到优秀水平,原麝潜在分布关键限制因子及其阈值为最干季平均温度<-3.6℃,到道路的距离>4100 m,坡度>71.5°,降水量变化方差为81.5~85.0,最适为83.5,昼夜温差月均值<11.6℃,最适为9.5℃。原麝的适宜区主要分布在宜川县的东南部、黄龙县的东北部及与洛川交界处、韩城的西北部,中等适宜区主要分布在宜川县适宜区周边及县城的西南部、黄龙县适宜区周边及西北部和东部、洛川县的东北部、韩城的西北部适宜区周边。适宜区面积为677.75 km ^(2),中等适宜区为2797.75 km ^(2)。各县域适宜区面积从大到小依次为宜川、韩城、黄龙和洛川,中等适宜区为黄龙、宜川、洛川和韩城。建议加强原麝适宜区的重点保护以及中等适宜区的保护和管理,控制进山人员的数量并进行教育,减少对原麝的干扰和捕杀。同时,建议林业部门近期把宜川县的原麝适宜区调整到陕西黄龙山国家级自然保护区,远期在洛川、黄龙和宜川的交界处成立新的保护区。
基金The project supported by National Natural Science Foundation of China(81422052,81403017)Special Topic of the Major Subject of National Science and Technology(2012ZX09509-339107)+1 种基金Hunan Provincial Natural Science Foundation of China(13JJ1010)by Funds for Hunan Education Department Program(12K006)
文摘OBJECTIVE SNPs in lnc RNAs may alter the expression or secondary structure of lnc RNAs and then impact their functions.Whether lnc RNA SNPs affect the prognosis of acute myeloid leukemia(AML)remains unknown.To search the association between lnc RNA SNPs and AML outcomes,thirty tag SNPs in GAS5,H19,MALAT1,WT1-as and SRA were genotyped in313 AML patients.METHODS Survival analysis was performed in both AML patients recruited presently and GEO samples.The expression of GAS5 and TP63 was analyzed by real-time quantitative PCR.Dual-luciferase reporter gene assay was used to confirm the interactions between GAS5 rs55829688 and TP63.RESULTS Survival analysis indicated that rs55829688(T>C),located in GAS5 promoter,was significantly associated with the prognosis of AML.The average overall survival(OS)for patients with the rs55829688 CC genotype was significantly shorter than those carrying the rs55829688 T allele(P=0.018).Patients with rs55829688 CC genotype showed higher GAS5 expression in PBMCs than carriers of rs55829688T allele(P=0.025).Rs55829688 CC homozygotes also harbored a longer platelets recovery than those with rs55829688 T allele(P=0.040).In vitro study showed that GAS5 promoter harboring the rs55829688 C al ele showed marginal y increased reporter gene activity(P=0.054),and the promoter activity was increased by TP63 in a dose-dependent manner(P=0.001).Moreover,GAS5 expression was associated with AML OS in the GEO GSE12417 dataset,and GAS5 higher expression predict shorter OS(P=0.011).CONCLUSION Rs55829688 polymorphism could increase GAS5 expression by interacting with TP63 and was associated with worse OS in Chinese AML patients.
基金The project supported by National Naural Science Foundation of China(81422052)Special Topic of the Major Subject of National Science and Technology(2012ZX09509-107)Hunan Provincial Natural Science Foundation of China(13JJ1010)
文摘OBJECTIVE To evaluate the association between DNA(cytosine-5)-methyltransferase 3 alpha(DNMT3A)genetic polymorphisms and the disease prognosis of R882 mutation negative acute myeloid leukemia(AML)patients.METHODS DNMT3A 11 SNPs(rs11695471,rs2289195,rs734693,rs2276598,rs1465825,rs7590760,rs13401241,rs7581217,rs749131,rs41284843 and rs7560488)were genotyped using a MassA RRAY platform or Sanger sequencing method in 317 diagnostic non-FABM3 AML patients without R882 mutation from southern China.AML patients underwent combined chemotherapy with cytarabine and anthracyclines.Overall survival(OS)and Disease-free survival(DFS)as major end points were defined.The prognostic(median OS and DFS)evaluations were performed by Kaplan-Meier curve and Cox′s proportional hazard model.RESULTS We found that the rs2289195 G>A SNP could act as a poor prognostic predictor independently(HR=0.442,P=0.035 for OS;HR=0.431,P=0.031 for DFS),while the rs1465825 T>C SNP and rs7590760 G>C SNP appeared to predict independently poor prognosis for both OS(HR=1.453,P=0.037 for rs1465825;HR=1.584,P=0.063 for rs7590760)and DFS(HR=1.459,P=0.057 for rs1465825;HR=1.965,P=0.017for rs7590760).However,no significant associations between other DNMT3A polymorphisms and prognosis(OS in conjunction with DFS)were observed.CONCLUSION DNMT3A polymorphisms may be potential predictive markers for AML prognosis in R882 mutation negative patients,which might improve prognostic stratification of AML.
