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基于表型性状和SSR分子标记的草地早熟禾遗传多样性分析和分子身份证构建 被引量:17
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作者 程林涛 孙晓阳 +5 位作者 谢福春 宋航 蒋佳 朱慧森 白小明 陈雅君 《中国草地学报》 CSCD 北大核心 2022年第9期1-10,共10页
为明确草地早熟禾种质资源的遗传背景,选择26份本土资源和54份国外引进种质进行表型特征和遗传多样性分析,结果表明:叶宽、株高和绿度3个表型性状存在一定变异,但仍有部分种质差异不显著,仅利用表型性状很难进行准确的种质鉴定。采用40... 为明确草地早熟禾种质资源的遗传背景,选择26份本土资源和54份国外引进种质进行表型特征和遗传多样性分析,结果表明:叶宽、株高和绿度3个表型性状存在一定变异,但仍有部分种质差异不显著,仅利用表型性状很难进行准确的种质鉴定。采用40对SSR引物序列筛选出多态性扩增效果好的16对引物,对80份草地早熟禾种质资源进行PCR扩增,共扩增出127条带。SSR标记数据显示,16对引物共检测到123个多态性位点,多态性比率96.85%,Nei’s基因多样性指数和Shannon’s多态性信息指数合度的平均值分别为0.34和0.52。遗传相关系数和聚类、主坐标分析结果表明,80份草地早熟禾种质可分为两大类7个亚类。结合表型性状和分子标记结果,构建了80份草地早熟禾种质的字符串、条形码和二维码分子身份证,可为草地早熟禾种质资源的鉴定、合理利用和有效保护提供参考。 展开更多
关键词 草地早熟禾 SSR分子标记 遗传多样性 表型性状
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基于组学技术的草坪草抗逆性研究进展 被引量:6
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作者 陈雅君 孙晓阳 +4 位作者 李勋 王鹏 孙华山 谢福春 崔国文 《中国草地学报》 CSCD 北大核心 2018年第6期99-106,共8页
综述了国内外有关后基因组时代的转录组学、蛋白质组学和代谢组学技术在草坪草抗逆领域研究的最新进展。分析了在不同逆境下,草坪草基因、蛋白和代谢物等分子学层面上的应答调控特性,总结了三种组学技术和相关方法在草坪草上应用的优势... 综述了国内外有关后基因组时代的转录组学、蛋白质组学和代谢组学技术在草坪草抗逆领域研究的最新进展。分析了在不同逆境下,草坪草基因、蛋白和代谢物等分子学层面上的应答调控特性,总结了三种组学技术和相关方法在草坪草上应用的优势。根据研究现状,揭示组学技术在草坪草抗逆研究中的不足。建议采用多组学互作综合研究草坪草的抗逆特性和代谢规律,结合生物信息学技术在草坪草抗逆机制上进行探索。 展开更多
关键词 草坪草 转录组学 蛋白质组学 代谢组学 抗逆性
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草坪植物持绿性及相关研究进展 被引量:3
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作者 刘立志 孙晓阳 +3 位作者 谢福春 秦立刚 蒋佳 陈雅君 《中国草地学报》 CSCD 北大核心 2022年第8期109-114,共6页
草坪是景观绿化的主要组成元素,具有重要的生态功能和美学价值。草坪草色泽和持绿性是衡量草坪质量的重要指标,选择和培育绿期长、叶色浓绿的草坪草品种对草坪生产有重要意义。对国内外植物持绿性的功能和机理、持绿功能基因调控草坪草... 草坪是景观绿化的主要组成元素,具有重要的生态功能和美学价值。草坪草色泽和持绿性是衡量草坪质量的重要指标,选择和培育绿期长、叶色浓绿的草坪草品种对草坪生产有重要意义。对国内外植物持绿性的功能和机理、持绿功能基因调控草坪草色泽的机制研究进展进行系统综述,并提出了研究建议。 展开更多
关键词 持绿性 草坪 生理代谢 分子调控 研究进展
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Research Progress on Nitrogen Use and Plant Growth 被引量:6
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作者 Cui Xin Yan Qing-wei +3 位作者 Sun Jia-lin Xiao Shuang xie fu-chun Chen Ya-jun 《Journal of Northeast Agricultural University(English Edition)》 CAS 2014年第2期68-74,共7页
The application of nitrogen (N) fertilizers in agriculture has been increasing dramatically since 1970s. However, the over-fertilization causes could cause environmental problems, as well as low N use efficiency (... The application of nitrogen (N) fertilizers in agriculture has been increasing dramatically since 1970s. However, the over-fertilization causes could cause environmental problems, as well as low N use efficiency (NUE). Promoting NUE in plants and minimizing the environmental impacts of N fertilizers had been the focus of the current research. We reviewed the importance of N, N metabolism and plant growth, plant N physiology and the molecular aspect of N metabolism in this paper. The future development of N use and NUE of plants was also discussed. 展开更多
关键词 N N metabolism plant growth N use efficiency
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Effect of Drought Stress on Growth and Water Physiological Characteris­tics of Poa sibirica 被引量:3
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作者 Zhang Cui-ting xie fu-chun +6 位作者 Yin Hang Zhang Gao-yun Guo Zhi-xin Zuo Yang Zhao Wei Shah Saud Chen Ya-jun 《Journal of Northeast Agricultural University(English Edition)》 CAS 2021年第1期24-30,共7页
In order to develop the resources of native turfgrass,the morphological traits and drought resistance of native Siberian bluegrass(Poa sibirica,abbreviated as PS)was evaluated using the introduced Kentucky bluegrass&#... In order to develop the resources of native turfgrass,the morphological traits and drought resistance of native Siberian bluegrass(Poa sibirica,abbreviated as PS)was evaluated using the introduced Kentucky bluegrass'Midnight'(Poa pratensis,abbreviated as PP)as a control.Two water schemes were imposed to plants in this pot culture study in greenhouse.One was with drought stress persistent limiting water supply for 20 days,the other was re-hydrated until 14 days after drought.The leaf shape,turf color,water status and cell plasma membrane permeability were evaluated.Similar changing trends with these parameters were shown for both species,and there were not significant differences with most evaluations during drought and re-water periods.The values leaf width and length of PS were higher while leaf color intensity was slightly lower than that of PP,but the greenness of PS leaf was still visually acceptable.There were not significant differences with cell membrane stability between the two species.In comparison,the native wild species PS possessed the potential for to be domesticated into a new cultivar for turf industry. 展开更多
关键词 TURFGRASS DROUGHT morphological characteristics water status cell membrane permeability
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Photosynthetic Characteristics of Clerodendrum trichotomum Thumb. Responses to Drought, Salt and Water-logging Stresses 被引量:1
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作者 xie fu-chun Wang Hua-tian Qin Dong 《Journal of Northeast Agricultural University(English Edition)》 CAS 2017年第3期1-9,共9页
In order to reveal the photosynthetic characteristics of C. trichotomum responses to drought, salt and water-logging stresses, one-year-old potted seedlings were taken as materials, and the several stresses including ... In order to reveal the photosynthetic characteristics of C. trichotomum responses to drought, salt and water-logging stresses, one-year-old potted seedlings were taken as materials, and the several stresses including natural drought, submergence stress, water-logging and different salt treatments (0.2%, 0.4%, 0.6% and 0.8% NaCl) were carried out on August 15, 2012. The morphological and photosynthetic characteristics were observed and determined. The results showed that adverse enviromental stress had a significant effect on the morphological changes and photosynthetic characteristics of C. trichotomum. On the 14th day after natural drought, the leaves wilted and could not recovery at night, and 60% of the seedlings could recover after re-watering. From the 7th day to the 10th day after submergence stress treatment, the 2nd and the 3rd leaves at the base of 60% seedling turned yellow and the lenticels were observed. At the early stage of water-logging stress, white lenticels appeared at the base of seedlings, and the leaves wilted, chlorina and fallen off on the 8th day. A large number of leaves fallen off under 0.6% NaCl or more salt stress, and even the whole plant died. The chlorophyll content, net photosynthetic rate (Pn) and transpiration rate (Tr) decreased gradually with the stress process, such as 8 days after natural drought, less than 0.4% salt stress and water-logging stress, but the changes were not significant compared with those of the control. With the increase of the stress intensity and the prolonged time, the changes of photosynthetic index were significant. All the results indicated that C. trichotomum had a certain degree of tolerance to drought, water and salt, but it was not suitable for living, in water-logging condition for a long time. 展开更多
关键词 Clerodendrum trichotomum Thumb. natural drought submergence stress water-logging salt stress photosyntheticcharacteristic
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Effects of Low Temperature on Physiological Responses of Two White Clover Cultivars 被引量:1
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作者 Li Xun Wang Peng +5 位作者 xie fu-chun Sun Hua-shan Liu Ke-meng Guan Tong Fu Zhen-xuan Chen Ya-jun 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第2期13-19,共7页
In order to explore the effects of low temperature on the two different genotypes of white clover, electrical conductivity (EC), soluble sugar, malondialdehyde (MDA) and some other physiological indexes of plants ... In order to explore the effects of low temperature on the two different genotypes of white clover, electrical conductivity (EC), soluble sugar, malondialdehyde (MDA) and some other physiological indexes of plants were compared under laboratory conditions. Seedlings of Longping No.1 and Koala were undergone a series of degrading temperatures set in turn 25℃, 10℃, 5℃, 0℃, –5℃ and –10℃. The results showed that the activities of superoxide dismutase (SOD), electrical conductivity, soluble sugar, free proline and MDA increased obviously with the decrease of stress temperature, while the content of starch decreased, and the content of soluble protein increased first and then decreased during the process. Comprehensive assessments on physiological responses to low temperature stress indicated that the cultivar Longping No.1 had stronger cold resistance than that of cultivar Koala. 展开更多
关键词 low temperature stress soluble sugar malondialdehyde (MDA) physiological adaptability
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Mowing Height and Mowing Frequency Interactions on Turf Performance of Kentucky Bluegrass
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作者 Sun Xiao-yang Wang Peng +5 位作者 xie fu-chun Sun Hua-shan Guan Tong Zuo Yang Li Hong Chen Ya-jun 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第3期10-15,共6页
The mowing height and mowing frequency are the key factors that affect the turf quality and turfgrass growing ability. The purpose of this study was to reveal the alternative effcient interaction between mowing height... The mowing height and mowing frequency are the key factors that affect the turf quality and turfgrass growing ability. The purpose of this study was to reveal the alternative effcient interaction between mowing height and mowing frequency in Kentucky bluegrass turf management. The Kentucky bluegrass cultivar 'Kenblue' (Poa pratensis L.) was used to evaluate the interactions of mowing heights (H1, H4 and H7) and mowing frequencies (F2, F5 and F8) on the performance of turfgrass and turf physiological responses under the feld condition. The results showed that the treatment of H7×F8 had perfect infuence on the turf texture, root elongation, and also enhanced soluble sugar and free proline content in plants. While, the turf quality, color, uniformity and the increasing tiller numbers were performed more superior in the treatment of H4×F5 than other treatments. In all the interacting treatments, the relation between leaf green index and number of shoots (dm2) was positively correlated, thus, it could be speculated that the proper mowing height with frequency had a strong impact on the increasing of turfgrass tillers. On the whole, the interaction of H4×F5 on turf quality was the most excellent treatment in this experiment. 展开更多
关键词 Kentucky bluegrass TURF MOWING HEIGHT FREQUENCY
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