Object detection models based on convolutional neural networks(CNN)have achieved state-of-the-art performance by heavily rely on large-scale training samples.They are insufficient when used in specific applications,su...Object detection models based on convolutional neural networks(CNN)have achieved state-of-the-art performance by heavily rely on large-scale training samples.They are insufficient when used in specific applications,such as the detection of military objects,as in these instances,a large number of samples is hard to obtain.In order to solve this problem,this paper proposes the use of Gabor-CNN for object detection based on a small number of samples.First of all,a feature extraction convolution kernel library composed of multi-shape Gabor and color Gabor is constructed,and the optimal Gabor convolution kernel group is obtained by means of training and screening,which is convolved with the input image to obtain feature information of objects with strong auxiliary function.Then,the k-means clustering algorithm is adopted to construct several different sizes of anchor boxes,which improves the quality of the regional proposals.We call this regional proposal process the Gabor-assisted Region Proposal Network(Gabor-assisted RPN).Finally,the Deeply-Utilized Feature Pyramid Network(DU-FPN)method is proposed to strengthen the feature expression of objects in the image.A bottom-up and a topdown feature pyramid is constructed in ResNet-50 and feature information of objects is deeply utilized through the transverse connection and integration of features at various scales.Experimental results show that the method proposed in this paper achieves better results than the state-of-art contrast models on data sets with small samples in terms of accuracy and recall rate,and thus has a strong application prospect.展开更多
OBJECTIVE Tissue plasminogen activator(tPA) is the only approved pharmaco.logical therapy for acute brain ischaemia;however,a major limitation of tPA is the haemorrhagic trans.formation that follows tPA treatment.Here...OBJECTIVE Tissue plasminogen activator(tPA) is the only approved pharmaco.logical therapy for acute brain ischaemia;however,a major limitation of tPA is the haemorrhagic trans.formation that follows tPA treatment.Here,we determined whether nicotinamide mononucleotide(NMN),a key intermediate of nicotinamide adenine dinucleotide biosynthesis,affects tPA-induced haemorrhagic transformation.METHODS Middle cerebral artery occlusion(MCAO) was achieved in CD1 mice by introducing a filament to the left MCA for 5 h.When the filament was removed for reperfusion,tPA was infused via the tail vein.A single dose of NMN was injected i.p.(300 mg·kg^(-1)).Mice were killed at 24 h post ischaemia,and their brains were evaluated for brain infarction,oedema,haemoglobin content,apoptosis,neuroinflammation,blood-brain barrier(BBB) permeability,the expression of tight junction proteins(TJPs) and the activity/expression of MMPs.RESULTS In the mice infused with tPA at 5 h post ischaemia,there were significant increases in mortality,brain infarction,brain oedema,brain haemoglobin level,neural apoptosis,Iba-1 staining(microglia activation) and myeloperoxidase staining(neutrophil infiltration).All these tPA-induced alterations were significantly prevented by NMN administration.Mechanistically,the delayed tPA treatment increased BBB permeability by downregulating TJPs,including claudin-1,occludin and zonula occludens-1,and enhancing the activities and protein expression of MMP9 and MMP2.Similarly,NMN administration partly blocked these tPAinduced molecular changes.CONCLUSION Our results demonstrate that NMN ameliorates tPAinduced haemorrhagic transformation in brain ischaemia by maintaining the integrity of the BBB.展开更多
基金supported by the National Natural Science Foundation of China(grant number:61671470)the National Key Research and Development Program of China(grant number:2016YFC0802904)the Postdoctoral Science Foundation Funded Project of China(grant number:2017M623423).
文摘Object detection models based on convolutional neural networks(CNN)have achieved state-of-the-art performance by heavily rely on large-scale training samples.They are insufficient when used in specific applications,such as the detection of military objects,as in these instances,a large number of samples is hard to obtain.In order to solve this problem,this paper proposes the use of Gabor-CNN for object detection based on a small number of samples.First of all,a feature extraction convolution kernel library composed of multi-shape Gabor and color Gabor is constructed,and the optimal Gabor convolution kernel group is obtained by means of training and screening,which is convolved with the input image to obtain feature information of objects with strong auxiliary function.Then,the k-means clustering algorithm is adopted to construct several different sizes of anchor boxes,which improves the quality of the regional proposals.We call this regional proposal process the Gabor-assisted Region Proposal Network(Gabor-assisted RPN).Finally,the Deeply-Utilized Feature Pyramid Network(DU-FPN)method is proposed to strengthen the feature expression of objects in the image.A bottom-up and a topdown feature pyramid is constructed in ResNet-50 and feature information of objects is deeply utilized through the transverse connection and integration of features at various scales.Experimental results show that the method proposed in this paper achieves better results than the state-of-art contrast models on data sets with small samples in terms of accuracy and recall rate,and thus has a strong application prospect.
基金supported by National Natural Science Foundation of China(8142204981473208+3 种基金816734858137341481130061) National 863 Plan Young Scientist Program of China(2015AA020943)
文摘OBJECTIVE Tissue plasminogen activator(tPA) is the only approved pharmaco.logical therapy for acute brain ischaemia;however,a major limitation of tPA is the haemorrhagic trans.formation that follows tPA treatment.Here,we determined whether nicotinamide mononucleotide(NMN),a key intermediate of nicotinamide adenine dinucleotide biosynthesis,affects tPA-induced haemorrhagic transformation.METHODS Middle cerebral artery occlusion(MCAO) was achieved in CD1 mice by introducing a filament to the left MCA for 5 h.When the filament was removed for reperfusion,tPA was infused via the tail vein.A single dose of NMN was injected i.p.(300 mg·kg^(-1)).Mice were killed at 24 h post ischaemia,and their brains were evaluated for brain infarction,oedema,haemoglobin content,apoptosis,neuroinflammation,blood-brain barrier(BBB) permeability,the expression of tight junction proteins(TJPs) and the activity/expression of MMPs.RESULTS In the mice infused with tPA at 5 h post ischaemia,there were significant increases in mortality,brain infarction,brain oedema,brain haemoglobin level,neural apoptosis,Iba-1 staining(microglia activation) and myeloperoxidase staining(neutrophil infiltration).All these tPA-induced alterations were significantly prevented by NMN administration.Mechanistically,the delayed tPA treatment increased BBB permeability by downregulating TJPs,including claudin-1,occludin and zonula occludens-1,and enhancing the activities and protein expression of MMP9 and MMP2.Similarly,NMN administration partly blocked these tPAinduced molecular changes.CONCLUSION Our results demonstrate that NMN ameliorates tPAinduced haemorrhagic transformation in brain ischaemia by maintaining the integrity of the BBB.
