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基于竞争性等位基因特异性PCR技术对啤酒花进行纯度检测 被引量:2
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作者 蒋培基 王德良 +4 位作者 江伟 宋涛 李涛 蒲彪 栾春光 《食品与发酵工业》 CAS CSCD 北大核心 2018年第1期211-216,共6页
基于已知数据库中啤酒花的基因组信息和测序信息设计引物,筛选出了可以区分7个啤酒花品种的8个多态性良好的单核苷酸多态性(single nucleotide polymorphisms,SNP)位点。并基于竞争性等位基因特异性PCR(Kompetitive allele specific PCR... 基于已知数据库中啤酒花的基因组信息和测序信息设计引物,筛选出了可以区分7个啤酒花品种的8个多态性良好的单核苷酸多态性(single nucleotide polymorphisms,SNP)位点。并基于竞争性等位基因特异性PCR(Kompetitive allele specific PCR,KASP)技术,利用筛选到的特异SNP标记对8份预混样品与2份企业送检样品进行了检测。结果表明,该技术能对混杂程度低至5%的啤酒花样品进行有效鉴定,可以满足企业对实际啤酒花样品进行检测评价的要求。研究结果完善了啤酒花的SNP数据库,为啤酒企业对于原料质量的把控提供有效的技术手段。 展开更多
关键词 啤酒花 单核苷酸多态性 基因特异性PCR 真实性检测 纯度检测
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A case study on fine weather in Western Antarctic Peninsula
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作者 Yaorong Huang(黄耀荣) +5 位作者 Cong Xu(许淙) tao Yin(尹涛) Haiying Zhang(张海影) 《海洋预报》 北大核心 2001年第z1期78-85,共8页
Based on observational data and facsimile charts from Dec. 1994 to Nov. 1995 of Chinese Antarctic Great Wall Station, the local fine weather was studied. Pressure, temperature and humidity field, and aerological strat... Based on observational data and facsimile charts from Dec. 1994 to Nov. 1995 of Chinese Antarctic Great Wall Station, the local fine weather was studied. Pressure, temperature and humidity field, and aerological stratification were analyzed synoptically. Two kinds of fine weather with different physical characteristics were found; one was caused by the spreading of subtropical high with high tem- perature and high humidity, and another was connected with polar high with low temperature and low humidity. The research provide a synoptic background for the local fine weather forecast. 展开更多
关键词 GREAT Wall Station western ANTARCTIC Peninsula FINE weather.
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