@Szakacs G$Technical University of Budapest, Department of Agricultural Chemical Technology, 1111 Budapest, Gellert ter 4, Hungar@Tavaszi A$Technical University of Budapest, Department of Agricultural Chemical Techn...@Szakacs G$Technical University of Budapest, Department of Agricultural Chemical Technology, 1111 Budapest, Gellert ter 4, Hungar@Tavaszi A$Technical University of Budapest, Department of Agricultural Chemical Technology, 1111 Budapest, Gellert ter 4,展开更多
The relationship between taxonomic status of Trichoderma spp., chitinase production in solid substrate fermentation (SSF) on four media and mycoparasitism in dual culture (confrontation assay) against four plant patho...The relationship between taxonomic status of Trichoderma spp., chitinase production in solid substrate fermentation (SSF) on four media and mycoparasitism in dual culture (confrontation assay) against four plant pathogenic fungi was studied. Seventy five Trichoderma isolates belonging to 35 species have been screened. The plant pathogenic fungi used in confrontation assay were Botrytis cinerea, Fusarium oxysporum f.sp. dianthi, Rhizoctonia solani and Sclerotinia sclerotiorum . The SSF media contained wheat bran, crude chitin (from crab shells, SIGMA) and salt solutions. The best performing isolates in mycoparasitism tests were Trichoderma flavofuscum, T.harzianum, T.inhamatum, T.koningii and T.strigosum. Some isolates exhibiting good mycoparasitism produced chitinase in SSF only at low or medium level. In contrary there were isolates with excellent extracellular chitinase production but their biocontrol potential did not belong to the leading group. Statistical methods have been used to evaluate the data.展开更多
Culture collection of the Technical University of Budapest (TUB) comprises app ro x. 2800 microorganisms with special emphasis on lignocellulose degrading fungi a nd actinomycetes. For isolation and screening programs...Culture collection of the Technical University of Budapest (TUB) comprises app ro x. 2800 microorganisms with special emphasis on lignocellulose degrading fungi a nd actinomycetes. For isolation and screening programs, 460 soils are stored at +5 ℃. They were collected in 58 countries, including extreme environments. There are 860 Trichoderma strains in TUB collection, representing 45 count ries and 173 soils and/or tree barks as isolation sources. Trichoderma spp . selected from soils in the tundra region (Rocky Mountains, Colorado) grow at l ow temperatures such as 5-10 ℃. These strains practically do not grow at 30 ℃ . On the contrary, many Trichoderma strains were isolated from tropical or s ubtropical soils and decaying plant materials at 35-40 ℃ in Brazil, Peru, Cost a Rica, Guatemala, Hawaii, Tanzania, Thailand and Indonesia. Trichoderma str ains tolerating limited oxygen supply were selected from river muds and sediment s. Trichoderma spp. tolerating or partially degrading 2,4-dichlorophenol a nd 2,4,6-trichlorophenol in 20 mg/kg concentration were selected from polluted soils. As a part of an attempt to assess the global biodiversity of Trichoderma, researchers of Technical University of Vienna and Agriculture Canada, in collab oration with TUB collection, identified and characterized seven new assumed taxa of Trichoderma from Eurasia.展开更多
文摘@Szakacs G$Technical University of Budapest, Department of Agricultural Chemical Technology, 1111 Budapest, Gellert ter 4, Hungar@Tavaszi A$Technical University of Budapest, Department of Agricultural Chemical Technology, 1111 Budapest, Gellert ter 4,
文摘The relationship between taxonomic status of Trichoderma spp., chitinase production in solid substrate fermentation (SSF) on four media and mycoparasitism in dual culture (confrontation assay) against four plant pathogenic fungi was studied. Seventy five Trichoderma isolates belonging to 35 species have been screened. The plant pathogenic fungi used in confrontation assay were Botrytis cinerea, Fusarium oxysporum f.sp. dianthi, Rhizoctonia solani and Sclerotinia sclerotiorum . The SSF media contained wheat bran, crude chitin (from crab shells, SIGMA) and salt solutions. The best performing isolates in mycoparasitism tests were Trichoderma flavofuscum, T.harzianum, T.inhamatum, T.koningii and T.strigosum. Some isolates exhibiting good mycoparasitism produced chitinase in SSF only at low or medium level. In contrary there were isolates with excellent extracellular chitinase production but their biocontrol potential did not belong to the leading group. Statistical methods have been used to evaluate the data.
文摘Culture collection of the Technical University of Budapest (TUB) comprises app ro x. 2800 microorganisms with special emphasis on lignocellulose degrading fungi a nd actinomycetes. For isolation and screening programs, 460 soils are stored at +5 ℃. They were collected in 58 countries, including extreme environments. There are 860 Trichoderma strains in TUB collection, representing 45 count ries and 173 soils and/or tree barks as isolation sources. Trichoderma spp . selected from soils in the tundra region (Rocky Mountains, Colorado) grow at l ow temperatures such as 5-10 ℃. These strains practically do not grow at 30 ℃ . On the contrary, many Trichoderma strains were isolated from tropical or s ubtropical soils and decaying plant materials at 35-40 ℃ in Brazil, Peru, Cost a Rica, Guatemala, Hawaii, Tanzania, Thailand and Indonesia. Trichoderma str ains tolerating limited oxygen supply were selected from river muds and sediment s. Trichoderma spp. tolerating or partially degrading 2,4-dichlorophenol a nd 2,4,6-trichlorophenol in 20 mg/kg concentration were selected from polluted soils. As a part of an attempt to assess the global biodiversity of Trichoderma, researchers of Technical University of Vienna and Agriculture Canada, in collab oration with TUB collection, identified and characterized seven new assumed taxa of Trichoderma from Eurasia.
