以新疆石河子地区月季花为试材,采用Illumina(Mi Seq)平台进行26S r DNA高通量测序分析、α多样性分析、可培养酵母菌的分离纯化技术(稀释涂布法和孢子弹射法)、26S r DNA D1/D区测序鉴定、糖发酵及碳同化等生理生化实验,研究了月季花...以新疆石河子地区月季花为试材,采用Illumina(Mi Seq)平台进行26S r DNA高通量测序分析、α多样性分析、可培养酵母菌的分离纯化技术(稀释涂布法和孢子弹射法)、26S r DNA D1/D区测序鉴定、糖发酵及碳同化等生理生化实验,研究了月季花上附生酵母菌(可培养和不可培养)菌群多样性,并对系统进化进行分析,以期为有效开发利用新疆酵母菌资源奠定基础,并为探索花蜜微生物与植物相互作用提供理论依据。结果表明:经26S r DNA高通量测序筛选后得到41 337个优化序列,划分入3 078个OTU,月季附生酵母菌群落结构丰富,鉴定为3个门、10个纲、13个目、22个科、37个属、90个种。担子菌门(Basidiomycota)为优势门、占18.9%,线黑粉菌属(Filobasidium)为优势属、占12.2%,酵母菌种群的丰度存在差异;可培养的酵母菌共鉴定出3个酵母菌属4个已知种群,分别为季也蒙毕赤酵母(Meyerozyma guilliermondii)、粘性红酵母(Rhodotorula mucilaginosa)、乌兹别克斯坦隐球酵母(Cryptococcus uzbekistanensis)、浅白隐球酵母(Cryptococcus albidus),优势酵母属为隐球酵母属(Cryptococcus)。展开更多
We analyse the porous structure of the packed beds in the heat transfer test facility built for high temperature gas cooled reactors from several aspects,such as oscillatory porosity,average porosity,thickness effect,...We analyse the porous structure of the packed beds in the heat transfer test facility built for high temperature gas cooled reactors from several aspects,such as oscillatory porosity,average porosity,thickness effect,coordination number and contact angle.An understanding and comparison of the porous structure of the facility bed and the real reactor core are developed to make recommendations for the design and analysis of the heat transfer test facility.The results show that there is very little difference between the porous characteristics of the two packed beds of spheres.展开更多
文摘以新疆石河子地区月季花为试材,采用Illumina(Mi Seq)平台进行26S r DNA高通量测序分析、α多样性分析、可培养酵母菌的分离纯化技术(稀释涂布法和孢子弹射法)、26S r DNA D1/D区测序鉴定、糖发酵及碳同化等生理生化实验,研究了月季花上附生酵母菌(可培养和不可培养)菌群多样性,并对系统进化进行分析,以期为有效开发利用新疆酵母菌资源奠定基础,并为探索花蜜微生物与植物相互作用提供理论依据。结果表明:经26S r DNA高通量测序筛选后得到41 337个优化序列,划分入3 078个OTU,月季附生酵母菌群落结构丰富,鉴定为3个门、10个纲、13个目、22个科、37个属、90个种。担子菌门(Basidiomycota)为优势门、占18.9%,线黑粉菌属(Filobasidium)为优势属、占12.2%,酵母菌种群的丰度存在差异;可培养的酵母菌共鉴定出3个酵母菌属4个已知种群,分别为季也蒙毕赤酵母(Meyerozyma guilliermondii)、粘性红酵母(Rhodotorula mucilaginosa)、乌兹别克斯坦隐球酵母(Cryptococcus uzbekistanensis)、浅白隐球酵母(Cryptococcus albidus),优势酵母属为隐球酵母属(Cryptococcus)。
基金Supported by the National Science and Technology Major Project under Grant No ZX06901the National Natural Science Foundation of China under Grant No 11072131.
文摘We analyse the porous structure of the packed beds in the heat transfer test facility built for high temperature gas cooled reactors from several aspects,such as oscillatory porosity,average porosity,thickness effect,coordination number and contact angle.An understanding and comparison of the porous structure of the facility bed and the real reactor core are developed to make recommendations for the design and analysis of the heat transfer test facility.The results show that there is very little difference between the porous characteristics of the two packed beds of spheres.
