【目的】以贯筋藤为原料,探究其提取物对小鼠迟发型超敏反应(delayed type hypersensitivity,DTH)的影响.【方法】通过乙醇辅助超声萃取的方法制备贯筋藤粗提物,采用泼尼松连续灌胃建立免疫抑制小鼠模型,以2,4-二硝基氟苯诱导DTH,检测...【目的】以贯筋藤为原料,探究其提取物对小鼠迟发型超敏反应(delayed type hypersensitivity,DTH)的影响.【方法】通过乙醇辅助超声萃取的方法制备贯筋藤粗提物,采用泼尼松连续灌胃建立免疫抑制小鼠模型,以2,4-二硝基氟苯诱导DTH,检测贯筋藤提取物对模型小鼠耳肿胀度的影响,计算胸腺和脾脏指数,酶联免疫吸附法检测小鼠血清干扰素-γ(IFN-γ)含量.【结果】明显减轻正常免疫小鼠耳廓肿胀度(P<0.01),对免疫抑制小鼠有减轻趋势,但无显著性差异(P>0.05);对小鼠胸腺和脾脏指数的影响无显著性差异(P>0.05);显著性降低正常免疫小鼠血清IFN-γ含量(P<0.05),对免疫抑制小鼠有降低趋势,但没有统计学差异(P>0.05).【结论】贯筋藤提取物对DTH小鼠具有明显的免疫抑制作用,其作用机制可能是通过抑制淋巴细胞分泌IFN-γ水平.展开更多
Ferrocene-based porous organic polymer(FcPOP) was constructed with ferrocene and porphyrin derivatives as building blocks via Schiff-base coupling. FcPOP was well characterized, and exhibited good thermal stability, h...Ferrocene-based porous organic polymer(FcPOP) was constructed with ferrocene and porphyrin derivatives as building blocks via Schiff-base coupling. FcPOP was well characterized, and exhibited good thermal stability, high porosity, microporous structure, and homogeneous pore size distribution. Ferrocene blocks with highly electron-rich characteristics endowed Fc POP with excellent adsorption capacity of CO2 and methyl violet. The kinetic study indicated adsorption of methyl violet onto FcPOP mainly complied with pesudo-second order model. The maximum adsorption capacity of FcPOP derived from Langmuir isotherm model reached up to 516 mg/g. More importantly, FcPOP could be easily regenerated and repeatedly employed for removal of methyl violet with high efficiency. Overall, FcPOP in the present study highlighted prospective applications in the field of gas capture and dyeing wastewater treatment.展开更多
文摘开展了取代5-(2-卤代苯基)-1 H四唑与炔烃为原料在镍(Ⅱ)盐催化串联反应生成四氮唑[5,1-α]异喹啉衍生物的微波辅助合成研究。结果表明:相同反应时间,在镍催化微波辅助条件下,生成四氮唑[5,1-α]异喹啉衍生物的效率较常规加热条件下增强。一系列四氮唑[5,1-α]异喹啉衍生物被高效地合成出来,最高产率达到88%。产物结构经1H NMR、13 C NMR及MS得到确证。该方法具有产率高、操作简单、环境友好等优点。
文摘【目的】以贯筋藤为原料,探究其提取物对小鼠迟发型超敏反应(delayed type hypersensitivity,DTH)的影响.【方法】通过乙醇辅助超声萃取的方法制备贯筋藤粗提物,采用泼尼松连续灌胃建立免疫抑制小鼠模型,以2,4-二硝基氟苯诱导DTH,检测贯筋藤提取物对模型小鼠耳肿胀度的影响,计算胸腺和脾脏指数,酶联免疫吸附法检测小鼠血清干扰素-γ(IFN-γ)含量.【结果】明显减轻正常免疫小鼠耳廓肿胀度(P<0.01),对免疫抑制小鼠有减轻趋势,但无显著性差异(P>0.05);对小鼠胸腺和脾脏指数的影响无显著性差异(P>0.05);显著性降低正常免疫小鼠血清IFN-γ含量(P<0.05),对免疫抑制小鼠有降低趋势,但没有统计学差异(P>0.05).【结论】贯筋藤提取物对DTH小鼠具有明显的免疫抑制作用,其作用机制可能是通过抑制淋巴细胞分泌IFN-γ水平.
基金Project(51778226)supported by the National Natural Science Foundation of ChinaProject(2018JJ3159)supported by the Hunan Provincial Natural Science Foundation for Youths,China。
文摘Ferrocene-based porous organic polymer(FcPOP) was constructed with ferrocene and porphyrin derivatives as building blocks via Schiff-base coupling. FcPOP was well characterized, and exhibited good thermal stability, high porosity, microporous structure, and homogeneous pore size distribution. Ferrocene blocks with highly electron-rich characteristics endowed Fc POP with excellent adsorption capacity of CO2 and methyl violet. The kinetic study indicated adsorption of methyl violet onto FcPOP mainly complied with pesudo-second order model. The maximum adsorption capacity of FcPOP derived from Langmuir isotherm model reached up to 516 mg/g. More importantly, FcPOP could be easily regenerated and repeatedly employed for removal of methyl violet with high efficiency. Overall, FcPOP in the present study highlighted prospective applications in the field of gas capture and dyeing wastewater treatment.
基金Project(51778226)supported by the National Natural Science Foundation of ChinaProject(2018JJ3159)supported by the Hunan Provincial Natural Science Foundation for Youths,China。
