Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocel...Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocellulose degradation by white-rot fimgi cultured on rapeseed straw. Fourier transform infrared spectroscopic analysis was used to show that the white-rot fungus Bjerkandera sp. strain increase the susceptibility of straw to enzymatic saccharification by modifying the lignin component, revealing the effect of these pretreatments on enzymatic saccharification. Reducing sugar production from straws pretreated by ammonia/mechanical chipping/fungi degradation was 29.80% higher than the samples treated with ammonia/autoclaving, indicating an effective degradation of phenolic compounds. After ammonia pretreatment 41% of the straw was converted to RS （reducing sugars） （glucose 50%）. After 5 weeks pretreatment with the white-rot fungus, 54.8% of rapeseed straw was further converted to RS, 74% of which was glucose; while only 12% of the control straw was converted （glucose 42%）. The white-rot fungus Bjerkandera sp. strain degraded rapeseed straw preferentially at the early stage （before 20 d）, and the degradation selectivity was 0.181 1 （cellulose）, 0.364 1 （hemicellulose）, and 0.454 8 （lignin）, suggesting that removal of the phenolic barriers enhanced reducing sugar yield, and the efficiency of fungal pretreatment was comparable with that after alkali treatment, resulting a higher proportion of glucose in the hydrolysates.展开更多
基金supported by the New & Renewable Energy of the Korea Institute of Energy Technology Evaluation and Planning(KETEP)Grant Funded by the Korea Government Ministry of Knowledge Economy(No.20103020090020)
文摘Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocellulose degradation by white-rot fimgi cultured on rapeseed straw. Fourier transform infrared spectroscopic analysis was used to show that the white-rot fungus Bjerkandera sp. strain increase the susceptibility of straw to enzymatic saccharification by modifying the lignin component, revealing the effect of these pretreatments on enzymatic saccharification. Reducing sugar production from straws pretreated by ammonia/mechanical chipping/fungi degradation was 29.80% higher than the samples treated with ammonia/autoclaving, indicating an effective degradation of phenolic compounds. After ammonia pretreatment 41% of the straw was converted to RS （reducing sugars） （glucose 50%）. After 5 weeks pretreatment with the white-rot fungus, 54.8% of rapeseed straw was further converted to RS, 74% of which was glucose; while only 12% of the control straw was converted （glucose 42%）. The white-rot fungus Bjerkandera sp. strain degraded rapeseed straw preferentially at the early stage （before 20 d）, and the degradation selectivity was 0.181 1 （cellulose）, 0.364 1 （hemicellulose）, and 0.454 8 （lignin）, suggesting that removal of the phenolic barriers enhanced reducing sugar yield, and the efficiency of fungal pretreatment was comparable with that after alkali treatment, resulting a higher proportion of glucose in the hydrolysates.
