In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40...In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40 Gy,and one flower color mutant was obtained.The morphological characteristics,physiological aspects,and DNA polymorphisms between wild-type and flower color mutants were analyzed.The colors of petal,peduncle,pistil,and stamen of the mutant displayed significant differences compared to those of the wild-type.Compared to the original plants,the total anthocyanin content in the petals of the mutant significantly decreased,resulting in a light pink petal phenotype.DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2%.The present study demonstrates that carbon ion beam irradiation is effective in inducing genomic variations,resulting in flower color geranium mutants within a relatively short period of time.Meanwhile,the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.展开更多
Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions ...Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions of the synergistic responses of NHX, AKT1, and SOS1 related to Na^+ accumulation were investigated in control(KFJT-CK) sorghum and KF1210-3 and KF1210-4 mutants. The results indicated that the NHX, AKT1, and SOS1 proteins in sweet sorghum are mainly involved in the transport, exclusion, and spatial distribution of Na^+,respectively. In addition to physiological parameters, we also measured the expression levels of NHX, AKT1, and SOS1 genes. The experimental results indicated that 150 m M Na Cl induced marked increases in the transcripts of NHX and SOS1 after 8 and 12 h in the KF1210-3,KF1210-4, and KFJT-CK cultivars. In contrast, however, a decrease in AKT1 was observed. On the basis of our results, we propose a model in which cooperation amongNHX, AKT1, and SOS1 facilitates Na^+ homeostasis in sweet sorghum in response to an increase in salt concentration. Accordingly, study of the regulatory mechanisms in sweet sorghum generated by carbon ion irradiation is essential for the selection of salt-tolerant cultivars.展开更多
Soil salinity can limit plant growth and productivity. The cultivation of tolerant varieties is convenient and cost-effective for making good use of the saline soils.The sweet sorghum plant has a high tolerance for sa...Soil salinity can limit plant growth and productivity. The cultivation of tolerant varieties is convenient and cost-effective for making good use of the saline soils.The sweet sorghum plant has a high tolerance for saline alkaline soils. The KF1210-3 and KF1210-4 early-maturity mutants of sweet sorghum were obtained via carbon ion irradiation. The study assesses the productivity of three sweet sorghum cultivars(KF1210-3, KF1210-4, and KFJTCK) which were grown in intermediate(4.6 d S m^(-1)) and high(11.9 d S m^(-1)) soil salinity. The sweet sorghum grown in the soil salinity of 4.6 d S m^(-1)produced 50.00–57.30 %greater fresh weight than that in the soil salinity of11.9 d S m^(-1), while the difference was not as obvious among the dry biomass of the three sweet sorghum cultivars. Moreover, the Brix degree of the sweet sorghum grown in the soil salinity of 11.9 d S m^(-1)was greater than that grown in the soil salinity of 4.6 d S m^(-1). The heavy ion irradiation experiment is of great significance in screening plant mutants, improving environmental conditions, and assessing the productivity. This process, in turn, aids in the understanding of the effects of the biochemical and physiological mechanisms of salt stress.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11205218,11275171,and 11405234)the Knowledge Innovation Project of the Chinese Academy of Sciences(CAS)(No.KJCX2-EW-N05)+1 种基金CAS‘‘Light of West China’’Program(No.29Y506020)the Youth Innovation Promotion Association of CAS(No.29Y506030)supported this study
文摘In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40 Gy,and one flower color mutant was obtained.The morphological characteristics,physiological aspects,and DNA polymorphisms between wild-type and flower color mutants were analyzed.The colors of petal,peduncle,pistil,and stamen of the mutant displayed significant differences compared to those of the wild-type.Compared to the original plants,the total anthocyanin content in the petals of the mutant significantly decreased,resulting in a light pink petal phenotype.DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2%.The present study demonstrates that carbon ion beam irradiation is effective in inducing genomic variations,resulting in flower color geranium mutants within a relatively short period of time.Meanwhile,the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.
基金supported by the Science and Technology Service Network Initiative(STS)program of the Chinese Academy of Sciences(CAS)(KFJ-EW-STS-086)the National Natural Science Foundation of China(No.11275171)+1 种基金the CAS‘‘Light of West China’’ Program(Nos.29Y506020 and 29Y406020)the Youth Innovation Promotion Association of CAS(No.2015337)
文摘Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions of the synergistic responses of NHX, AKT1, and SOS1 related to Na^+ accumulation were investigated in control(KFJT-CK) sorghum and KF1210-3 and KF1210-4 mutants. The results indicated that the NHX, AKT1, and SOS1 proteins in sweet sorghum are mainly involved in the transport, exclusion, and spatial distribution of Na^+,respectively. In addition to physiological parameters, we also measured the expression levels of NHX, AKT1, and SOS1 genes. The experimental results indicated that 150 m M Na Cl induced marked increases in the transcripts of NHX and SOS1 after 8 and 12 h in the KF1210-3,KF1210-4, and KFJT-CK cultivars. In contrast, however, a decrease in AKT1 was observed. On the basis of our results, we propose a model in which cooperation amongNHX, AKT1, and SOS1 facilitates Na^+ homeostasis in sweet sorghum in response to an increase in salt concentration. Accordingly, study of the regulatory mechanisms in sweet sorghum generated by carbon ion irradiation is essential for the selection of salt-tolerant cultivars.
基金supported by the STS Project(KFJ-EW-STS-086)the Western Light Co-scholar(29Y406020)Program of the Chinese Academy of Sciences
文摘Soil salinity can limit plant growth and productivity. The cultivation of tolerant varieties is convenient and cost-effective for making good use of the saline soils.The sweet sorghum plant has a high tolerance for saline alkaline soils. The KF1210-3 and KF1210-4 early-maturity mutants of sweet sorghum were obtained via carbon ion irradiation. The study assesses the productivity of three sweet sorghum cultivars(KF1210-3, KF1210-4, and KFJTCK) which were grown in intermediate(4.6 d S m^(-1)) and high(11.9 d S m^(-1)) soil salinity. The sweet sorghum grown in the soil salinity of 4.6 d S m^(-1)produced 50.00–57.30 %greater fresh weight than that in the soil salinity of11.9 d S m^(-1), while the difference was not as obvious among the dry biomass of the three sweet sorghum cultivars. Moreover, the Brix degree of the sweet sorghum grown in the soil salinity of 11.9 d S m^(-1)was greater than that grown in the soil salinity of 4.6 d S m^(-1). The heavy ion irradiation experiment is of great significance in screening plant mutants, improving environmental conditions, and assessing the productivity. This process, in turn, aids in the understanding of the effects of the biochemical and physiological mechanisms of salt stress.
