【目的】揭示刺豚鼠信号蛋白基因(ASIP)SNP位点突变与不同鸡种羽色差异形成机制的关联性,筛选出与鸡种羽色相关的辅助育种分子标记。【方法】参考NCBI已公布的红色原鸡ASIP基因序列(登录号NC_006107.5),运用Primer Premier 5.0设计特异...【目的】揭示刺豚鼠信号蛋白基因(ASIP)SNP位点突变与不同鸡种羽色差异形成机制的关联性,筛选出与鸡种羽色相关的辅助育种分子标记。【方法】参考NCBI已公布的红色原鸡ASIP基因序列(登录号NC_006107.5),运用Primer Premier 5.0设计特异性引物,以罗曼蛋鸡、泰和乌骨鸡、兴义矮脚鸡白羽系、赤水乌骨鸡、贵州黄鸡和瑶山鸡为研究对象,通过DNA混合池及PCR直接测序法筛选不同羽色鸡种ASIP基因SNP位点;估算各SNP位点等位基因频率后,使用RNAfold、NetPhos 2.0、NetOGlyc 4.0、SOPMA和SWISS-MODEL等在线软件进行生物信息学分析,探究不同羽色鸡种ASIP基因SNP位点突变对基因mRNA二级结构、ASIP蛋白翻译后修饰位点及其二、三级结构的影响。【结果】从6个鸡种ASIP基因中检测到7个SNPs位点,分别为Exon-2-G17C、Exon-2-T168C、Exon-2-C3032T、Exon-2-T3203G、Exon-2-G3287A、Exon-2-G3313T和Exon-2-C3350T。其中,Exon-2-T3203G只出现在罗曼蛋鸡上,Exon-2-G3287A只出现在瑶山鸡上。Exon-2-T168C只出现在贵州黄鸡和兴义矮脚鸡白羽系且位于编码区,该位点突变导致编码氨基酸改变[苏氨酸(Thr)→甲硫氨酸(Met)],且能导致ASIP基因mRNA二级结构改变,最小自由能增加,稳定性降低。Exon-2-T168C位点突变还导致ASIP蛋白激酶磷酸化位点数量和位置发生改变,但糖基化位点未发生改变;Exon-2-T168C位点突变后ASIP蛋白二级结构中α-螺旋、β-转角和延伸链的占比增加,而无规则卷曲占比降低,且蛋白三级结构预测结果与二级结构预测结果一致。【结论】在贵州黄鸡和兴义矮脚鸡白羽系ASIP基因上检测到Exon-2-T168C位点,且该位点突变能引起ASIP基因m RNA二级结构、蛋白激酶磷酸化位点数量和位置及ASIP蛋白二、三级结构均发生改变,但并不是影响贵州黄鸡和兴义矮脚鸡白羽系羽色差异的内在机制。展开更多
The gas-liquid two-phase swirl flow can increase the gas-liquid two-phase contact area and enhance the heat and mass transfer efficiency between gas and liquid.The swirl flow has important practical application value ...The gas-liquid two-phase swirl flow can increase the gas-liquid two-phase contact area and enhance the heat and mass transfer efficiency between gas and liquid.The swirl flow has important practical application value for promoting gas hydrate formation and ensuring the flow safe of natural gas hydrate slurry.The experimental section was made of plexiglass pipe and the experimental medium was air and water.The flow pattern of the gas-liquid two-phase swirl flow in the horizontal pipe was divided,according to a high-definition camera and the overall characteristics of the gas-liquid interface.The flow pattern map of the gas-liquid two-phase swirl flow in a horizontal pipe was studied.The influence of the flow velocity and vane parameters on pressure drop was investigated.Two types of gas-liquid two-phase swirl flow pressure drop models was established.The homogeneous-phase and split-phase pressure drop models have good prediction on swirl bubble flow,swirl dispersed flow,swirl annular flow and swirl stratified flow,and the predictive error band is not more than 20%.展开更多
基金Project(51574045)supported by the National Nature Science Foundation of China
文摘The gas-liquid two-phase swirl flow can increase the gas-liquid two-phase contact area and enhance the heat and mass transfer efficiency between gas and liquid.The swirl flow has important practical application value for promoting gas hydrate formation and ensuring the flow safe of natural gas hydrate slurry.The experimental section was made of plexiglass pipe and the experimental medium was air and water.The flow pattern of the gas-liquid two-phase swirl flow in the horizontal pipe was divided,according to a high-definition camera and the overall characteristics of the gas-liquid interface.The flow pattern map of the gas-liquid two-phase swirl flow in a horizontal pipe was studied.The influence of the flow velocity and vane parameters on pressure drop was investigated.Two types of gas-liquid two-phase swirl flow pressure drop models was established.The homogeneous-phase and split-phase pressure drop models have good prediction on swirl bubble flow,swirl dispersed flow,swirl annular flow and swirl stratified flow,and the predictive error band is not more than 20%.
