以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方...以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方法提取的mtDNA浓度和纯度较高,无叶绿体和核基因组DNA的污染,可用于后续大豆线粒体基因组的相关研究。展开更多
As a kind of high-efficiency explosive with compound destructive capability, the energy output law of thermobaric explosives has been receiving great attention. In order to investigate the effects of main components o...As a kind of high-efficiency explosive with compound destructive capability, the energy output law of thermobaric explosives has been receiving great attention. In order to investigate the effects of main components on the explosive characteristics of thermobaric explosives, various high explosives and oxidants were selected to formulate five different types of thermobaric explosive. Then they were tested in both open space and closed space respectively. Pressure measurement system, high-speed camera,infrared thermal imager and multispectral temperature measurement system were used for pressure,temperature and fireball recording. The effects of different components on the explosive characteristics of thermobaric explosive were analyzed. The results showed that in open space, the overpressure is dominated by the high explosives content in the formulation. The addition of the oxidants will decrease the explosion overpressure but will increase the duration and overall brightness of the fireball. While in closed space, the quasi-static pressure formed after the explosion is positively correlated with the temperature and gas production. In addition, it was found that the differences in shell constraints can also alter the afterburning reaction of thermobaric explosives, thus affecting their energy output characteristics. PVC shell constraint obviously increases the overpressure and makes the fireball burn more violently.展开更多
文摘以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方法提取的mtDNA浓度和纯度较高,无叶绿体和核基因组DNA的污染,可用于后续大豆线粒体基因组的相关研究。
基金the support of the National Natural Science Foundation of China(Grant No.12302440)China Postdoctoral Science Foundation(Grant No.2023M741713)。
文摘As a kind of high-efficiency explosive with compound destructive capability, the energy output law of thermobaric explosives has been receiving great attention. In order to investigate the effects of main components on the explosive characteristics of thermobaric explosives, various high explosives and oxidants were selected to formulate five different types of thermobaric explosive. Then they were tested in both open space and closed space respectively. Pressure measurement system, high-speed camera,infrared thermal imager and multispectral temperature measurement system were used for pressure,temperature and fireball recording. The effects of different components on the explosive characteristics of thermobaric explosive were analyzed. The results showed that in open space, the overpressure is dominated by the high explosives content in the formulation. The addition of the oxidants will decrease the explosion overpressure but will increase the duration and overall brightness of the fireball. While in closed space, the quasi-static pressure formed after the explosion is positively correlated with the temperature and gas production. In addition, it was found that the differences in shell constraints can also alter the afterburning reaction of thermobaric explosives, thus affecting their energy output characteristics. PVC shell constraint obviously increases the overpressure and makes the fireball burn more violently.
