在挤出机单螺杆计量段二维解析建模的基础上,采用交叉验证方法构建人工神经网络(artificial neural network,ANN)模型并对其进行了超参数优化,以有效地映射挤出机工作条件和结构参数与生产率和功耗之间的复杂非线性关系。提出利用ANN代...在挤出机单螺杆计量段二维解析建模的基础上,采用交叉验证方法构建人工神经网络(artificial neural network,ANN)模型并对其进行了超参数优化,以有效地映射挤出机工作条件和结构参数与生产率和功耗之间的复杂非线性关系。提出利用ANN代理模型,结合NSGA-Ⅱ(non-dominated sorting genetic algorithmⅡ)算法对螺杆计量段的结构参数进行多目标优化,并通过TOPSIS(technique for order preference by similarity to an ideal solution)法得到最优生产率和功耗组合的结构参数。相关工作对单螺杆计量段结构参数的智能化设计具有理论指导意义。展开更多
采用电弧离子镀技术在高速钢基底上沉积Cr Al N涂层.对Cr Al N涂层的表面形貌、微观组织、显微硬度、结合强度、摩擦学性能进行了分析,研究了负偏压对Cr Al N涂层组织和性能的影响.结果表明:在一定范围内随着负偏压的增加,涂层表面大颗...采用电弧离子镀技术在高速钢基底上沉积Cr Al N涂层.对Cr Al N涂层的表面形貌、微观组织、显微硬度、结合强度、摩擦学性能进行了分析,研究了负偏压对Cr Al N涂层组织和性能的影响.结果表明:在一定范围内随着负偏压的增加,涂层表面大颗粒数量逐渐减少,涂层变得更加致密;但过大的负偏压导致离子轰击作用过强,使涂层表面再次出现缺陷.当负偏压为-200 V时,涂层的晶粒尺寸最小,并具有良好的结晶度.涂层的显微硬度和结合强度均随负偏压的增加呈现出先增加后减小的趋势.当负偏压为-200 V时,显微硬度达到最大值,为28.6 GPa,同时具有最好的摩擦学性能.展开更多
OBJECTIVE o facilitate the basic acquaintance with the bioactive lycodine-type alkaloids biosynthetic pathways,we conducted the transcriptome analysis of L.casuarinoides by illumina sequencing.METHODS The plant of L.c...OBJECTIVE o facilitate the basic acquaintance with the bioactive lycodine-type alkaloids biosynthetic pathways,we conducted the transcriptome analysis of L.casuarinoides by illumina sequencing.METHODS The plant of L.casuarinoides was collected and was subjected to RNA isolation,cDNA library construction,and illumina sequencing before bioinformatics analysis.After sequencing,the clean reads were obtained for de novo assembly by using Trinity software,and then further processed with TGICL sequencing clustering software to generate unigenes,The unigenes are aligned by Blast X alignment to six public protein database.In addition,all unigenes are functionally annotated by GO,KEGG and characterized putative genes involved in lycopodium alkaloids biosynthesis.RESULTS In total,124,524 high-quality unigenes were obtained with an average sequence length of 601 bp.Among the L.casuarinoides transcripts,61,304 showed significant similarity(E-value<1 e-5) to the known proteins in the public database.Among the total 124 524 unigenes,47,538 open reading frame(ORFs) were predicted.Based on the bioinformatics analysis,all possible enzymes involved in the Lycodine-type alkaloids biosynthetic pathway of L.casuarinoides were identified,including primary amine oxidase(PAO),and Malonly-CoA decarboxylase.In addition,a total of 64 putative cytochrome P450(CYP450) and 827 transcription factors were selected as the candidates of Lycodine-type alka.loids modifiers.Furthermore,a total of 13 352 simple sequence repeats(SSRs) were identified from the 124,524 unigenes,of which dinucleotide motifs AG/CT(50.1%),were the most abundant.CONCLU.SION This transcriptome analysis of L.casuarinoides,provides many valuable candidate genes involv.ing in the biosynthesis of novel secondary metabolites but also lays the foundation for genetic diversity analysis via SSRs markers in L.casuarinoides.展开更多
文摘在挤出机单螺杆计量段二维解析建模的基础上,采用交叉验证方法构建人工神经网络(artificial neural network,ANN)模型并对其进行了超参数优化,以有效地映射挤出机工作条件和结构参数与生产率和功耗之间的复杂非线性关系。提出利用ANN代理模型,结合NSGA-Ⅱ(non-dominated sorting genetic algorithmⅡ)算法对螺杆计量段的结构参数进行多目标优化,并通过TOPSIS(technique for order preference by similarity to an ideal solution)法得到最优生产率和功耗组合的结构参数。相关工作对单螺杆计量段结构参数的智能化设计具有理论指导意义。
文摘采用电弧离子镀技术在高速钢基底上沉积Cr Al N涂层.对Cr Al N涂层的表面形貌、微观组织、显微硬度、结合强度、摩擦学性能进行了分析,研究了负偏压对Cr Al N涂层组织和性能的影响.结果表明:在一定范围内随着负偏压的增加,涂层表面大颗粒数量逐渐减少,涂层变得更加致密;但过大的负偏压导致离子轰击作用过强,使涂层表面再次出现缺陷.当负偏压为-200 V时,涂层的晶粒尺寸最小,并具有良好的结晶度.涂层的显微硬度和结合强度均随负偏压的增加呈现出先增加后减小的趋势.当负偏压为-200 V时,显微硬度达到最大值,为28.6 GPa,同时具有最好的摩擦学性能.
基金supported by Jiangxi′s Major International Science and Technology Cooperation Projects(20151BDH80020)
文摘OBJECTIVE o facilitate the basic acquaintance with the bioactive lycodine-type alkaloids biosynthetic pathways,we conducted the transcriptome analysis of L.casuarinoides by illumina sequencing.METHODS The plant of L.casuarinoides was collected and was subjected to RNA isolation,cDNA library construction,and illumina sequencing before bioinformatics analysis.After sequencing,the clean reads were obtained for de novo assembly by using Trinity software,and then further processed with TGICL sequencing clustering software to generate unigenes,The unigenes are aligned by Blast X alignment to six public protein database.In addition,all unigenes are functionally annotated by GO,KEGG and characterized putative genes involved in lycopodium alkaloids biosynthesis.RESULTS In total,124,524 high-quality unigenes were obtained with an average sequence length of 601 bp.Among the L.casuarinoides transcripts,61,304 showed significant similarity(E-value<1 e-5) to the known proteins in the public database.Among the total 124 524 unigenes,47,538 open reading frame(ORFs) were predicted.Based on the bioinformatics analysis,all possible enzymes involved in the Lycodine-type alkaloids biosynthetic pathway of L.casuarinoides were identified,including primary amine oxidase(PAO),and Malonly-CoA decarboxylase.In addition,a total of 64 putative cytochrome P450(CYP450) and 827 transcription factors were selected as the candidates of Lycodine-type alka.loids modifiers.Furthermore,a total of 13 352 simple sequence repeats(SSRs) were identified from the 124,524 unigenes,of which dinucleotide motifs AG/CT(50.1%),were the most abundant.CONCLU.SION This transcriptome analysis of L.casuarinoides,provides many valuable candidate genes involv.ing in the biosynthesis of novel secondary metabolites but also lays the foundation for genetic diversity analysis via SSRs markers in L.casuarinoides.
