Objectives This study examined the protective effect of salubrinal and the mechanism underlying this protection against tunicamycin （TM）- and hypoxia-induced apoptosis in rat cardiomyocytes. Methods Neonatal rat car...Objectives This study examined the protective effect of salubrinal and the mechanism underlying this protection against tunicamycin （TM）- and hypoxia-induced apoptosis in rat cardiomyocytes. Methods Neonatal rat cardiomyocytes were cultured from the ventricles of l-day-old Wistar rats. Cells were exposed to different concentrations of salubrinal （10, 20, and 40 gmol/L） for 30 min followed by TM treatment or hypoxia for 36 h. Apoptosis was measured by a multiparameter HCS （high content screening） apoptosis assay, TUNEL assay and flow cytometry. The phosphorylation of eukaryotic translation initiation factor 2 subunit alpha （eIF2c0 and the expression of cleaved caspase-12 were determined by Western blotting. C/EBP homologous protein （CHOP） was detected by immunocytochemistry. Results HCS, TUNEL assays and flow cytometry showed that salubrinal protected cardiomyocytes against apoptosis induced by TM or hypoxia. Western blotting showed that salubrinal protected cardiomyocytes against apoptosis by inducing eIF2ct phosphorylation and down-regulating the expression of the endoplasmic reticulum stress-mediated apoptotic proteins, CHOP and cleaved caspase-12. Conclusions Our study suggests that salubrinal protects rat cardiomyocytes against TM- or hypoxia-associated apoptosis via a mechanism involving the inhibition of ER stress-mediated apoptosis.展开更多
Lithium-sulfur(Li-S)batteries with high theoretical specific energy of 2600 Wh kg^(-1) are one of promising candidates for next-generation energy storage devices.However,the severe shuttle effect of intermediate polys...Lithium-sulfur(Li-S)batteries with high theoretical specific energy of 2600 Wh kg^(-1) are one of promising candidates for next-generation energy storage devices.However,the severe shuttle effect of intermediate polysulfides leads to rapid capacity decay during battery cycling,especially at high sulfur loading and high current density.Herein,the MnO nanoparticles covered carbon with endoplasmic-reticulum-like structure(MnO@ERC)as separator coating for Li-S batteries is proposed.The MnO@ERC coating can act as upper current collector to enhance electrical conductivity of cathode and decrease the interface impedance of the whole battery.More importantly,both the polar MnO nanoparticles and Mn_(3)O_(4) formed at the end of the charging process can catalyze the conversion of lithium polysulfides,which is convinced by the high adsorption energy and the elongate S–S bond.As a result,Li-S batteries based on MnO@ERC coating separator showed stable cycle for 350 cycles under 0.5C,high discharge specific capacity of 783.6m Ah g^(-1) after 100 cycles at 0.2 C,534.7 m Ah g^(-1) after 100 cycles under the sulfur loading of 5.26 mg cm;and low self-discharge rate of 9.1%after resting 48 h..展开更多
A coupled(2+1)-dimensional variable coefficient Ginzburg-Landau equation is studied.By virtue of the modified Hirota bilinear method,the bright one-soliton solution of the equation is derived.Some phenomena of soliton...A coupled(2+1)-dimensional variable coefficient Ginzburg-Landau equation is studied.By virtue of the modified Hirota bilinear method,the bright one-soliton solution of the equation is derived.Some phenomena of soliton propagation are analyzed by setting different dispersion terms.The influences of the corresponding parameters on the solitons are also discussed.The results can enrich the soliton theory,and may be helpful in the manufacture of optical devices.展开更多
Objective To explore the effect of α-galactosyleramide （α-GalCer） on immune reconstitution under acute graft-versus-host disease （aGVHD）. Methods BALB/c mice were transplanted with allogeneic C57BL/6 bone marro...Objective To explore the effect of α-galactosyleramide （α-GalCer） on immune reconstitution under acute graft-versus-host disease （aGVHD）. Methods BALB/c mice were transplanted with allogeneic C57BL/6 bone marrow cells and splenocytes （both 1 × 10^7） after receiving lethal total-body irradiation, α-GalCer （100 ug/kg） or vehicle （dimethyl- sulfoxide） was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitufion, proliferation of T cells and B cells, hematopoiesis, and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3^＋, CD4^＋, CD8^＋, B220^＋, CD40＋, and CD86＋cells compared with the vehicle group. The number of colony forming unit per 1000 CD34^＋ cells in the et-GalCer group was higher than in the vehicle group （P=0.0012）. In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3^＋, CD4^＋, CD8^＋, and B220^＋ cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3^＋, CD4^＋, CD8^＋, and B220^＋ cells were higher in the α-GalCer group than in the normal group, especially the number of B220^＋ cells （P=0.007）. Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3^＋, CD4^＋, and CD8^＋ cells. Conclusion Administration of tl-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.展开更多
We report a new minimally invasive technique utilizing interventional ultrasound for precise external intracerebral hemorrhage drain(EICHD) placement in pigs.
A passively Q-switched Nd：YVO4 laser at 1064 nm is demonstrated based on a gold nanotriangle saturable absorber（GNT SA）.Under a pump power of 3.82 W,the maximum average output power of 218mW is achieved,correspondi...A passively Q-switched Nd：YVO4 laser at 1064 nm is demonstrated based on a gold nanotriangle saturable absorber（GNT SA）.Under a pump power of 3.82 W,the maximum average output power of 218mW is achieved,corresponding to a slope efficiency of 12.9%.The minimum pulse width is 165ns and the maximum pulse repetition rate is 300kHz at the pump power of 3.48 W.Our results prove that the GNT SA is a promising saturable absorber for near-infrared lasers.展开更多
In the aging populations of China,there is an increasing prevalence of various types of bradycardias that require pacing therapy.In 1958,the first transvenous pacemaker(TVPM)was implanted at Karolinska Hospital in Swe...In the aging populations of China,there is an increasing prevalence of various types of bradycardias that require pacing therapy.In 1958,the first transvenous pacemaker(TVPM)was implanted at Karolinska Hospital in Sweden,which unveiled the era of cardiac pacing therapy.[1]A TVPM includes transvenous electrodes and a pulse gener-ator that is implanted subcutaneously.Despite its obvious benefits,the complication rate associated with TVPM could be as high as 10%.展开更多
基金This study was supported by the Ministry Science Foundation of the Chinese People's Liberation Army during the 12th Five-Year Plan Period
文摘Objectives This study examined the protective effect of salubrinal and the mechanism underlying this protection against tunicamycin （TM）- and hypoxia-induced apoptosis in rat cardiomyocytes. Methods Neonatal rat cardiomyocytes were cultured from the ventricles of l-day-old Wistar rats. Cells were exposed to different concentrations of salubrinal （10, 20, and 40 gmol/L） for 30 min followed by TM treatment or hypoxia for 36 h. Apoptosis was measured by a multiparameter HCS （high content screening） apoptosis assay, TUNEL assay and flow cytometry. The phosphorylation of eukaryotic translation initiation factor 2 subunit alpha （eIF2c0 and the expression of cleaved caspase-12 were determined by Western blotting. C/EBP homologous protein （CHOP） was detected by immunocytochemistry. Results HCS, TUNEL assays and flow cytometry showed that salubrinal protected cardiomyocytes against apoptosis induced by TM or hypoxia. Western blotting showed that salubrinal protected cardiomyocytes against apoptosis by inducing eIF2ct phosphorylation and down-regulating the expression of the endoplasmic reticulum stress-mediated apoptotic proteins, CHOP and cleaved caspase-12. Conclusions Our study suggests that salubrinal protects rat cardiomyocytes against TM- or hypoxia-associated apoptosis via a mechanism involving the inhibition of ER stress-mediated apoptosis.
基金supported by the National Natural Science Foundation of China,China(51772030,51972030)the Beijing Institute of Technology Research Fund Program for Young Scholars,Chinathe Beijing Outstanding Young Scientists Program,China(BJJWZYJH01201910007023)。
文摘Lithium-sulfur(Li-S)batteries with high theoretical specific energy of 2600 Wh kg^(-1) are one of promising candidates for next-generation energy storage devices.However,the severe shuttle effect of intermediate polysulfides leads to rapid capacity decay during battery cycling,especially at high sulfur loading and high current density.Herein,the MnO nanoparticles covered carbon with endoplasmic-reticulum-like structure(MnO@ERC)as separator coating for Li-S batteries is proposed.The MnO@ERC coating can act as upper current collector to enhance electrical conductivity of cathode and decrease the interface impedance of the whole battery.More importantly,both the polar MnO nanoparticles and Mn_(3)O_(4) formed at the end of the charging process can catalyze the conversion of lithium polysulfides,which is convinced by the high adsorption energy and the elongate S–S bond.As a result,Li-S batteries based on MnO@ERC coating separator showed stable cycle for 350 cycles under 0.5C,high discharge specific capacity of 783.6m Ah g^(-1) after 100 cycles at 0.2 C,534.7 m Ah g^(-1) after 100 cycles under the sulfur loading of 5.26 mg cm;and low self-discharge rate of 9.1%after resting 48 h..
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11674036 and 11875008)Beijing Youth Top Notch Talent Support Program,China(Grant No.2017000026833ZK08)+1 种基金Fund of State Key Laboratory of Information Photonics and Optical Communications(Beijing University of Posts and Telecommunications,Grant No.IPOC2019ZZ01)Fundamental Research Funds for the Central Universities,China(Grant No.500419305).
文摘A coupled(2+1)-dimensional variable coefficient Ginzburg-Landau equation is studied.By virtue of the modified Hirota bilinear method,the bright one-soliton solution of the equation is derived.Some phenomena of soliton propagation are analyzed by setting different dispersion terms.The influences of the corresponding parameters on the solitons are also discussed.The results can enrich the soliton theory,and may be helpful in the manufacture of optical devices.
基金Supported by National Natural Science Foundation of China (30670898, 30572108)National Basic Research Program of China (973 Program) (2005CB522400)Capital Research Fund for Medical Development (2007-2040)
文摘Objective To explore the effect of α-galactosyleramide （α-GalCer） on immune reconstitution under acute graft-versus-host disease （aGVHD）. Methods BALB/c mice were transplanted with allogeneic C57BL/6 bone marrow cells and splenocytes （both 1 × 10^7） after receiving lethal total-body irradiation, α-GalCer （100 ug/kg） or vehicle （dimethyl- sulfoxide） was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitufion, proliferation of T cells and B cells, hematopoiesis, and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3^＋, CD4^＋, CD8^＋, B220^＋, CD40＋, and CD86＋cells compared with the vehicle group. The number of colony forming unit per 1000 CD34^＋ cells in the et-GalCer group was higher than in the vehicle group （P=0.0012）. In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3^＋, CD4^＋, CD8^＋, and B220^＋ cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3^＋, CD4^＋, CD8^＋, and B220^＋ cells were higher in the α-GalCer group than in the normal group, especially the number of B220^＋ cells （P=0.007）. Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3^＋, CD4^＋, and CD8^＋ cells. Conclusion Administration of tl-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.
基金supported by grants from the Beijing Science and Technology New Star Project (XX2018019/Z181100006218028)the National Natural Science Fund (81701961)+3 种基金the PLA Logistic Major Science and Technology Project (16QNP135)the PLA General Hospital Science and Technology Project (2018XXFC-20, 16KMM56, 2017FC-WJFWZX-30)the Winter Olympics of Science and Technology (2019YFF0302300)the Construction Project of Key Disciplines in the 13th Five-Year Plan of the PLA (Traumatic Surgery in the Battlefield, 2019–126, 2019–513)。
文摘We report a new minimally invasive technique utilizing interventional ultrasound for precise external intracerebral hemorrhage drain(EICHD) placement in pigs.
基金Supported by the National Natural Science Foundation of China under Grant No 61475086the Natural Science Foundation of Shandong Province under Grant Nos ZR2015FM018 and ZR2014FM028
文摘A passively Q-switched Nd：YVO4 laser at 1064 nm is demonstrated based on a gold nanotriangle saturable absorber（GNT SA）.Under a pump power of 3.82 W,the maximum average output power of 218mW is achieved,corresponding to a slope efficiency of 12.9%.The minimum pulse width is 165ns and the maximum pulse repetition rate is 300kHz at the pump power of 3.48 W.Our results prove that the GNT SA is a promising saturable absorber for near-infrared lasers.
文摘In the aging populations of China,there is an increasing prevalence of various types of bradycardias that require pacing therapy.In 1958,the first transvenous pacemaker(TVPM)was implanted at Karolinska Hospital in Sweden,which unveiled the era of cardiac pacing therapy.[1]A TVPM includes transvenous electrodes and a pulse gener-ator that is implanted subcutaneously.Despite its obvious benefits,the complication rate associated with TVPM could be as high as 10%.
