目的探讨敲低YTH结构域N^6甲基腺嘌呤(m^6A)RNA结合蛋白2(YTHDF2)对人宫颈癌细胞增殖、周期和凋亡的影响。方法利用人蛋白图谱(Human Protein Atlas)数据库分析YTHDF2在宫颈癌中表达及与生存时间的关系。收集宫颈癌和正常宫颈组织各31例...目的探讨敲低YTH结构域N^6甲基腺嘌呤(m^6A)RNA结合蛋白2(YTHDF2)对人宫颈癌细胞增殖、周期和凋亡的影响。方法利用人蛋白图谱(Human Protein Atlas)数据库分析YTHDF2在宫颈癌中表达及与生存时间的关系。收集宫颈癌和正常宫颈组织各31例,采用免疫组织化学法检测YTHDF2的蛋白表达差异;利用敲低YTHDF2的短发夹RNA(shRNA)及空载质粒包装慢病毒,并感染至宫颈癌HeLa细胞和SiHa细胞,实时荧光定量PCR及Western blot法检测YTHDF2的mRNA及蛋白水平,敲低YTHDF2后,采用CCK-8法检测细胞增殖活性,集落形成实验检测细胞集落形成能力;流式细胞术检测细胞周期和细胞凋亡情况。结果检测数据库发现YTHDF2在宫颈癌中表达越高生存时间越短,与正常宫颈组织相比,YTHDF2在宫颈癌组织高表达。敲低YTHDF2后,抑制宫颈癌细胞增殖,促进细胞凋亡,使细胞阻滞于S期。结论YTHDF2在宫颈癌组织中高表达,敲低后抑制宫颈癌细胞增殖并促进其凋亡。展开更多
A mixed method which combines the recent developed finite analytical method and the boundary fitting coordinate transformation method was used first to calculate the temperature field and thermal stress field of the c...A mixed method which combines the recent developed finite analytical method and the boundary fitting coordinate transformation method was used first to calculate the temperature field and thermal stress field of the cold roller in this paper. The following results are obtained by the calculation: thermal stresses σr=0, σθ=σz are maximum tension stress on the inner surface of the cold roller and σr=0, |σθ|=|σz| are maximum compression stress on the outer surface of the cold roller in the steady and unsteady case. Effectiveness and validity of the mixed method are checked with steady coil roller problems having theoretical solutions. The results show that good agreement is achived between the calculated value and theoretical solution,and the mixed method used in the paper is very workable. The mixed method is also useful in solving the steady and unsteady thermal stress field proplems of the hot -rolled for the reversing rolling mill and the continous rolling mill.展开更多
In this paper, a distortion dynamic strain energy density factor criterion for dynamic mixed-mode fracture initiation in rock is suggested.The results of the investigation on 14 three-point bending (TPB) and four-poin...In this paper, a distortion dynamic strain energy density factor criterion for dynamic mixed-mode fracture initiation in rock is suggested.The results of the investigation on 14 three-point bending (TPB) and four-point bending(FPB) rock specimens tested u展开更多
文摘目的探讨敲低YTH结构域N^6甲基腺嘌呤(m^6A)RNA结合蛋白2(YTHDF2)对人宫颈癌细胞增殖、周期和凋亡的影响。方法利用人蛋白图谱(Human Protein Atlas)数据库分析YTHDF2在宫颈癌中表达及与生存时间的关系。收集宫颈癌和正常宫颈组织各31例,采用免疫组织化学法检测YTHDF2的蛋白表达差异;利用敲低YTHDF2的短发夹RNA(shRNA)及空载质粒包装慢病毒,并感染至宫颈癌HeLa细胞和SiHa细胞,实时荧光定量PCR及Western blot法检测YTHDF2的mRNA及蛋白水平,敲低YTHDF2后,采用CCK-8法检测细胞增殖活性,集落形成实验检测细胞集落形成能力;流式细胞术检测细胞周期和细胞凋亡情况。结果检测数据库发现YTHDF2在宫颈癌中表达越高生存时间越短,与正常宫颈组织相比,YTHDF2在宫颈癌组织高表达。敲低YTHDF2后,抑制宫颈癌细胞增殖,促进细胞凋亡,使细胞阻滞于S期。结论YTHDF2在宫颈癌组织中高表达,敲低后抑制宫颈癌细胞增殖并促进其凋亡。
文摘A mixed method which combines the recent developed finite analytical method and the boundary fitting coordinate transformation method was used first to calculate the temperature field and thermal stress field of the cold roller in this paper. The following results are obtained by the calculation: thermal stresses σr=0, σθ=σz are maximum tension stress on the inner surface of the cold roller and σr=0, |σθ|=|σz| are maximum compression stress on the outer surface of the cold roller in the steady and unsteady case. Effectiveness and validity of the mixed method are checked with steady coil roller problems having theoretical solutions. The results show that good agreement is achived between the calculated value and theoretical solution,and the mixed method used in the paper is very workable. The mixed method is also useful in solving the steady and unsteady thermal stress field proplems of the hot -rolled for the reversing rolling mill and the continous rolling mill.
文摘In this paper, a distortion dynamic strain energy density factor criterion for dynamic mixed-mode fracture initiation in rock is suggested.The results of the investigation on 14 three-point bending (TPB) and four-point bending(FPB) rock specimens tested u
