旨在探究影响猪达100 kg体重日龄(age at 100 kg,AGE)和达100 kg体重平均日增重(average daily gain at 100 kg,ADG)的候选基因。本研究采集了来自大白、长白和杜洛克3个品种的共计4593头健康成年猪的耳组织作为试验材料,其中公猪2563头...旨在探究影响猪达100 kg体重日龄(age at 100 kg,AGE)和达100 kg体重平均日增重(average daily gain at 100 kg,ADG)的候选基因。本研究采集了来自大白、长白和杜洛克3个品种的共计4593头健康成年猪的耳组织作为试验材料,其中公猪2563头,母猪2030头。通过记录猪只的日龄和体重,计算校正AGE和ADG。通过酚氯仿法提取样品DNA,利用“中芯一号”50K SNP芯片对样本进行基因分型,并对质控后的SNPs进行基因型填充,将SNPs位点数从4万填充至800万。随后,利用GEMMA混合线性模型对AGE和ADG性状进行填充前后的全基因组关联分析,使用BEDTools在显著位点上、下游1 Mb范围查找候选基因。同时,结合PigGTEx中的34个组织的表达量数量性状位点数据,使用R软件进行共定位分析,挖掘与GWAS信号共享同一因果变异的基因,通过GWAS和共定位分析,确定AGE和ADG性状的候选基因。GWAS分析结果显示,AGE与ADG性状的显著SNP为1号染色体上的1_270827213。在该位点上、下游1 Mb范围内,共鉴定到32个基因。共定位分析结果显示,对于AGE性状,有10个基因的eQTL信号与GWAS信号共定位。对于ADG性状,有11个基因的eQTL信号与GWAS信号共定位。最终,确定了CRAT、GPR 107和USP 20这3个基因作为AGE的候选基因,确定了CRAT、GPR107、USP20、FNBP1、PTGES和HMCN 2这6个基因作为ADG的候选基因。本研究为猪品种改良提供了分子标记,对猪生长相关性状功能基因挖掘奠定基础。展开更多
A pH predictive model based on acid–base equilibria and ion equilibria was proposed to calculate the pH value of the overhead condensing system of crude distillation unit. The pH model could be used to predict the ap...A pH predictive model based on acid–base equilibria and ion equilibria was proposed to calculate the pH value of the overhead condensing system of crude distillation unit. The pH model could be used to predict the appropriate amounts of neutralizers by measuring the Cl-ion concentration of the overhead knockout drum. The pH values of various neutralized streams were estimated by this model. The results showed that the predicted pH values were in good agreement with the experimental ones. The trend of the corrosion inhibition efficiency decreases in the following order: ethylenediamine > N,Ndimethylethanolamine> triethylamine > 3-methoxypropylamine > morpholine. The difficulty in the accurate control of corrosion was solved, and a good instruction was provided for mitigating corrosion in refinery.展开更多
基金supported by the PetroChina Company Limited (20151191)
文摘A pH predictive model based on acid–base equilibria and ion equilibria was proposed to calculate the pH value of the overhead condensing system of crude distillation unit. The pH model could be used to predict the appropriate amounts of neutralizers by measuring the Cl-ion concentration of the overhead knockout drum. The pH values of various neutralized streams were estimated by this model. The results showed that the predicted pH values were in good agreement with the experimental ones. The trend of the corrosion inhibition efficiency decreases in the following order: ethylenediamine > N,Ndimethylethanolamine> triethylamine > 3-methoxypropylamine > morpholine. The difficulty in the accurate control of corrosion was solved, and a good instruction was provided for mitigating corrosion in refinery.
