Background Cell metabolism plays a pivotal role in tumor progression,and targeting cancer metabolism might effectively kill cancer cells.We aimed to investigate the role of hexokinases in prostate cancer(PCa)and ident...Background Cell metabolism plays a pivotal role in tumor progression,and targeting cancer metabolism might effectively kill cancer cells.We aimed to investigate the role of hexokinases in prostate cancer(PCa)and identify a crucial target for PCa treatment.Methods The Cancer Genome Atlas(TCGA)database,online tools and clinical samples were used to assess the expression and prognostic role of ADP-dependent glucokinase(ADPGK)in PCa.The effect of ADPGK expression on PCa cell malignant phenotypes was validated in vitro and in vivo.Quantitative proteomics,metabolomics,and extracellular acidification rate(ECAR)and oxygen consumption rate(OCR)tests were performed to evaluate the impact of ADPGK on PCa metabolism.The underlying mechanisms were explored through ADPGK overexpression and knockdown,co-immunoprecipitation(Co-IP),ECAR analysis and cell counting kit-8(CCK-8)assays.Results ADPGK was the only glucokinase that was both upregulated and predicted worse overall survival(OS)in prostate adenocarcinoma(PRAD).Clinical sample analysis demonstrated that ADPGK was markedly upregulated in PCa tissues vs.non-PCa tissues.High ADPGK expression indicates worse survival outcomes,and ADPGK serves as an independent factor of biochemical recurrence.In vitro and in vivo experiments showed that ADPGK overexpression promoted PCa cell proliferation and migration,and ADPGK inhibition suppressed malignant phenotypes.Metabolomics,proteomics,and ECAR and OCR tests revealed that ADPGK significantly accelerated glycolysis in PCa.Mechanistically,ADPGK binds aldolase C(ALDOC)to promote glycolysis via AMP-activated protein kinase(AMPK)phosphorylation.ALDOC was positively correlated with ADPGK,and high ALDOC expression was associated with worse survival outcomes in PCa.Conclusions In summary,ADPGK is a driving factor in PCa progression,and its high expression contributes to a poor prognosis in PCa patients.ADPGK accelerates PCa glycolysis and progression by activating ALDOC-AMPK signaling,suggesting that ADPGK might be an effective target and marker for PCa treatment and prognosis evaluation.展开更多
The technique of electrocatalytic hydrogen evolution reaction (HER) represents a development trend of clean energy generation and conversion,while the electrode catalysts are bound to be the core unit in the electroch...The technique of electrocatalytic hydrogen evolution reaction (HER) represents a development trend of clean energy generation and conversion,while the electrode catalysts are bound to be the core unit in the electrochemical HER system.Herein,we demonstrate a bottom-up approach to the construction of three-dimensional (3D) interconnected ternary nanoarchitecture originated from Ti_(3)C_(2)T_(x)MXene,graphitic carbon nitride nanosheets and graphene (MX/CN/RGO) through a convenient co-assembly process.By virtue of the 3D porous frameworks with ultrathin walls,large specific surface areas,optimized electronic structures,high electric conductivity,the resulting MX/CN/RGO nanoarchitecture expresses an exceptional HER performance with a low onset potential of only 38 m V,a small Tafel slop of 76 m V dec^(-1) as well as long lifespan,all of which are more competitive than those of the bare Ti_(3)C_(2)T_(x),g-C_(3)N_(4),graphene as well as binary MX/RGO and CN/RGO electrocatalysts.Theoretical simulations further verify that the ternary MX/CN/RGO nanoarchitecture with ameliorative band structure is able to facilitate the electron transport and meanwhile offer multistage catalytically active sites,thereby guaranteeing rapid HER kinetics during the electrocatalytic process.展开更多
A packing-flotation column was proposed to optimize the flotation environment A research system was es- tablished using a 100 mm diameter cyclonic micro-bubble flotation column to study fluid properties. Dry-plate and...A packing-flotation column was proposed to optimize the flotation environment A research system was es- tablished using a 100 mm diameter cyclonic micro-bubble flotation column to study fluid properties. Dry-plate and wet- plate pressure drops were studied and the corresponding pressure drop equations developed. The results show that the dry-plate pressure drop of the packing cyclonic micro-bubble flotation column is 10–15 times less than that of the chemical tower, which is principally shown in its relatively small resistance coefficient, ξ ≈0.0207. The wet-plate pressure drop is 2–3 times higher than that of the chemical tower, which is largely caused by the separation materials and characteristics of the equipment. With flotation, the greater the pressure drop, the better the flotation environment.展开更多
基金National Key R&D Plan(2023YFC3403200)National Natural Science Foundation of China(82070784,81702536,81974099 and 82170785)+4 种基金Science&Technology Department of Sichuan Province,China(2022JDRC0040,21GJHZ0246)Young Investigator Award of Sichuan University 2017(2017SCU04A17)Sichuan University-Panzhihua Science and Technology Cooperation Special Fund(2020CDPZH-4)China Postdoctoral Science Foundation(2021M692306)Post-Doctor Research Project of West China Hospital of Sichuan University(2021HXBH025).
文摘Background Cell metabolism plays a pivotal role in tumor progression,and targeting cancer metabolism might effectively kill cancer cells.We aimed to investigate the role of hexokinases in prostate cancer(PCa)and identify a crucial target for PCa treatment.Methods The Cancer Genome Atlas(TCGA)database,online tools and clinical samples were used to assess the expression and prognostic role of ADP-dependent glucokinase(ADPGK)in PCa.The effect of ADPGK expression on PCa cell malignant phenotypes was validated in vitro and in vivo.Quantitative proteomics,metabolomics,and extracellular acidification rate(ECAR)and oxygen consumption rate(OCR)tests were performed to evaluate the impact of ADPGK on PCa metabolism.The underlying mechanisms were explored through ADPGK overexpression and knockdown,co-immunoprecipitation(Co-IP),ECAR analysis and cell counting kit-8(CCK-8)assays.Results ADPGK was the only glucokinase that was both upregulated and predicted worse overall survival(OS)in prostate adenocarcinoma(PRAD).Clinical sample analysis demonstrated that ADPGK was markedly upregulated in PCa tissues vs.non-PCa tissues.High ADPGK expression indicates worse survival outcomes,and ADPGK serves as an independent factor of biochemical recurrence.In vitro and in vivo experiments showed that ADPGK overexpression promoted PCa cell proliferation and migration,and ADPGK inhibition suppressed malignant phenotypes.Metabolomics,proteomics,and ECAR and OCR tests revealed that ADPGK significantly accelerated glycolysis in PCa.Mechanistically,ADPGK binds aldolase C(ALDOC)to promote glycolysis via AMP-activated protein kinase(AMPK)phosphorylation.ALDOC was positively correlated with ADPGK,and high ALDOC expression was associated with worse survival outcomes in PCa.Conclusions In summary,ADPGK is a driving factor in PCa progression,and its high expression contributes to a poor prognosis in PCa patients.ADPGK accelerates PCa glycolysis and progression by activating ALDOC-AMPK signaling,suggesting that ADPGK might be an effective target and marker for PCa treatment and prognosis evaluation.
基金financially supported by the National Natural Science Foundation of China(51802077)the Fundamental Research Funds for the Central Universities(B200202129 and B210202093)the Nantong Science and Technology Bureau(JC2019086 and JC2019003)。
文摘The technique of electrocatalytic hydrogen evolution reaction (HER) represents a development trend of clean energy generation and conversion,while the electrode catalysts are bound to be the core unit in the electrochemical HER system.Herein,we demonstrate a bottom-up approach to the construction of three-dimensional (3D) interconnected ternary nanoarchitecture originated from Ti_(3)C_(2)T_(x)MXene,graphitic carbon nitride nanosheets and graphene (MX/CN/RGO) through a convenient co-assembly process.By virtue of the 3D porous frameworks with ultrathin walls,large specific surface areas,optimized electronic structures,high electric conductivity,the resulting MX/CN/RGO nanoarchitecture expresses an exceptional HER performance with a low onset potential of only 38 m V,a small Tafel slop of 76 m V dec^(-1) as well as long lifespan,all of which are more competitive than those of the bare Ti_(3)C_(2)T_(x),g-C_(3)N_(4),graphene as well as binary MX/RGO and CN/RGO electrocatalysts.Theoretical simulations further verify that the ternary MX/CN/RGO nanoarchitecture with ameliorative band structure is able to facilitate the electron transport and meanwhile offer multistage catalytically active sites,thereby guaranteeing rapid HER kinetics during the electrocatalytic process.
基金Project 50425414 supported by the National Outstanding Youth Science Foundation of China
文摘A packing-flotation column was proposed to optimize the flotation environment A research system was es- tablished using a 100 mm diameter cyclonic micro-bubble flotation column to study fluid properties. Dry-plate and wet- plate pressure drops were studied and the corresponding pressure drop equations developed. The results show that the dry-plate pressure drop of the packing cyclonic micro-bubble flotation column is 10–15 times less than that of the chemical tower, which is principally shown in its relatively small resistance coefficient, ξ ≈0.0207. The wet-plate pressure drop is 2–3 times higher than that of the chemical tower, which is largely caused by the separation materials and characteristics of the equipment. With flotation, the greater the pressure drop, the better the flotation environment.
